ES2396426T3 - Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra - Google Patents

Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra Download PDF

Info

Publication number
ES2396426T3
ES2396426T3 ES05812037T ES05812037T ES2396426T3 ES 2396426 T3 ES2396426 T3 ES 2396426T3 ES 05812037 T ES05812037 T ES 05812037T ES 05812037 T ES05812037 T ES 05812037T ES 2396426 T3 ES2396426 T3 ES 2396426T3
Authority
ES
Spain
Prior art keywords
image guide
laser
sample
fiber
excitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES05812037T
Other languages
English (en)
Spanish (es)
Inventor
Bertrand Viellerobe
Francois Lacombe
Alexandre Loiseau
Frédéric LOURADOUR
Michaël LELEK
Alain Barthelemy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Limoges, Mauna Kea Technologies SA filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of ES2396426T3 publication Critical patent/ES2396426T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2476—Non-optical details, e.g. housings, mountings, supports
    • G02B23/2484—Arrangements in relation to a camera or imaging device
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • A61B5/0086—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters using infrared radiation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2407—Optical details
    • G02B23/2461—Illumination
    • G02B23/2469—Illumination using optical fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A61B5/0068—Confocal scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/105—Purely optical scan
    • G01N2201/1053—System of scan mirrors for composite motion of beam
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/04—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Surgery (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Astronomy & Astrophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Radiology & Medical Imaging (AREA)
  • Multimedia (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Endoscopes (AREA)
ES05812037T 2004-10-22 2005-10-21 Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra Expired - Lifetime ES2396426T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0411313A FR2877103B1 (fr) 2004-10-22 2004-10-22 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon
FR0411313 2004-10-22
PCT/FR2005/002630 WO2006045936A2 (fr) 2004-10-22 2005-10-21 Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon

Publications (1)

Publication Number Publication Date
ES2396426T3 true ES2396426T3 (es) 2013-02-21

Family

ID=34951919

Family Applications (1)

Application Number Title Priority Date Filing Date
ES05812037T Expired - Lifetime ES2396426T3 (es) 2004-10-22 2005-10-21 Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra

Country Status (8)

Country Link
US (1) US8237131B2 (enExample)
EP (1) EP1817620B8 (enExample)
JP (1) JP5102622B2 (enExample)
AU (1) AU2005298494B2 (enExample)
CA (1) CA2584748C (enExample)
ES (1) ES2396426T3 (enExample)
FR (1) FR2877103B1 (enExample)
WO (1) WO2006045936A2 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7715673B2 (en) 2005-06-16 2010-05-11 Swinburne University Of Technology Imaging system
US8554087B2 (en) 2006-09-18 2013-10-08 The Trustees Of Dartmouth College System and method for imaging objects through turbid media
DE102006046925A1 (de) * 2006-09-28 2008-04-03 Jenlab Gmbh Verfahren und Anordnung zur Laser-Endoskopie für die Mikrobearbeitung
JP5307439B2 (ja) * 2007-04-23 2013-10-02 オリンパス株式会社 レーザ顕微鏡
US8059274B2 (en) * 2007-12-07 2011-11-15 The Spectranetics Corporation Low-loss polarized light diversion
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
US8507877B2 (en) * 2009-11-25 2013-08-13 Ofs Fitel, Llc Non-linear optical system and techniques
US9304308B2 (en) * 2009-12-09 2016-04-05 Advanced Micro Devices, Inc. Laser scanning module including an optical isolator
US20110147615A1 (en) * 2009-12-23 2011-06-23 Kintz Gregory J Method and apparatus for microscopic imaging system with wide field of view and high collection efficiency
RS61066B1 (sr) * 2010-03-05 2020-12-31 Massachusetts Gen Hospital Sistemi koji obezbeđuju mikroskopske slike najmanje jedne anatomske strukture na određenoj rezoluciji
WO2012167201A1 (en) * 2011-06-01 2012-12-06 Digital Light Innovations System and method for hyperspectral illumination
WO2012170963A1 (en) 2011-06-08 2012-12-13 Digital Light Innovations System and method for hyperspectral imaging
US9356413B2 (en) * 2011-09-30 2016-05-31 Olympus Corporation Laser source apparatus and laser microscope
EP2785250A4 (en) * 2011-11-28 2015-07-29 Univ Leland Stanford Junior SYSTEM AND METHOD FOR SARCOME REPRESENTATION THROUGH LENS-BASED MICROSCOPY
WO2015108991A2 (en) 2014-01-17 2015-07-23 Imra America, Inc. Laser-based modification of transparent materials
JP2017513211A (ja) * 2014-02-28 2017-05-25 イムラ アメリカ インコーポレイテッド 顕微鏡に適用される多波長超短パルスの生成及び放出
DE112015001154T5 (de) * 2014-04-24 2016-12-08 Olympus Corporation Mikroskop und Mikroskopie-Verfahren
JP2015227778A (ja) * 2014-05-30 2015-12-17 ソニー株式会社 画像取得装置及び画像取得方法
FR3030956B1 (fr) * 2014-12-18 2018-03-23 Centre National De La Recherche Scientifique - Cnrs Dispositif de transport et de controle d'impulsions lumineuses pour l'imagerie endo-microscopique sans lentille
JP6594437B2 (ja) 2015-09-15 2019-10-23 オリンパス株式会社 顕微鏡および顕微鏡観察方法
JP7245051B2 (ja) 2016-03-08 2023-03-23 エンスペクトラ・ヘルス・インコーポレイテッド 皮膚の疾患の非侵襲的な検出
CN109310296A (zh) 2016-04-06 2019-02-05 爱丁堡大学董事会 内窥镜成像装置及方法
US9645291B1 (en) * 2016-04-18 2017-05-09 Ii-Vi Incorporated Voltage-tunable optical filters for instrumentation applications
WO2018201082A1 (en) 2017-04-28 2018-11-01 Zebra Medical Technologies, Inc. Systems and methods for imaging and measurement of sarcomeres
GB201707239D0 (en) 2017-05-05 2017-06-21 Univ Edinburgh Optical system and method
WO2020010330A1 (en) * 2018-07-06 2020-01-09 The Trustees Of Columbia University In The City Of New York Collagen tissue modification
WO2020102442A1 (en) 2018-11-13 2020-05-22 Enspectra Health, Inc. Methods and systems for generating depth profiles
CN109656014B (zh) * 2019-01-31 2024-03-19 北京超维景生物科技有限公司 多路荧光收集装置及三维非线性激光扫描腔体内窥镜
CN113349708B (zh) * 2021-06-08 2023-05-26 之江实验室 一种基于大色散镜头的双模态高分辨大深度显微内窥成像系统
KR102567260B1 (ko) * 2021-09-01 2023-08-16 연세대학교 산학협력단 망막 진단 시스템 및 이를 이용한 망막 진단방법
CN114755210A (zh) * 2022-04-22 2022-07-15 波粒光子(苏州)精密光电科技有限公司 高性能光纤荧光探测器及其生产方法
CN119523389B (zh) * 2024-11-20 2025-09-23 卓外（上海）医疗电子科技有限公司 一种基于谐波成像和多光子荧光成像的内窥镜系统

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4655547A (en) * 1985-04-09 1987-04-07 Bell Communications Research, Inc. Shaping optical pulses by amplitude and phase masking
JP3269713B2 (ja) * 1993-09-03 2002-04-02 株式会社日立製作所 光伝送システム
DE19622359B4 (de) 1996-06-04 2007-11-22 Carl Zeiss Jena Gmbh Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
US5862287A (en) 1996-12-13 1999-01-19 Imra America, Inc. Apparatus and method for delivery of dispersion compensated ultrashort optical pulses with high peak power
US6249630B1 (en) * 1996-12-13 2001-06-19 Imra America, Inc. Apparatus and method for delivery of dispersion-compensated ultrashort optical pulses with high peak power
US5995281A (en) * 1997-04-09 1999-11-30 Carl Zeiss Jena Gmbh Device for coupling the radiation of short-pulse lasers in an optical beam path of a microscope
GB2338568B (en) 1998-06-19 2000-12-20 Optiscan Pty Ltd Two photon endoscope or microscope method and apparatus
US6449039B1 (en) * 1999-07-28 2002-09-10 Thermo Noran Inc. Laser scanning fluorescence microscopy with compensation for spatial dispersion of fast laser pulses
US6369928B1 (en) 2000-11-01 2002-04-09 Optical Biopsy Technologies, Inc. Fiber-coupled, angled-dual-illumination-axis confocal scanning microscopes for performing reflective and two-photon fluorescence imaging
JP2003344777A (ja) * 2002-05-24 2003-12-03 Japan Science & Technology Corp 光ファイバ顕微鏡および内視鏡
FR2842407B1 (fr) * 2002-07-18 2005-05-06 Mauna Kea Technologies "procede et appareillage d'imagerie de fluorescence confocale fibree"
FR2842628B1 (fr) 2002-07-18 2004-09-24 Mauna Kea Technologies "procede de traitement d'une image acquise au moyen d'un guide compose d'une pluralite de fibres optiques"

Also Published As

Publication number Publication date
EP1817620B8 (fr) 2012-12-12
US8237131B2 (en) 2012-08-07
AU2005298494B2 (en) 2011-08-11
AU2005298494A1 (en) 2006-05-04
WO2006045936A2 (fr) 2006-05-04
JP5102622B2 (ja) 2012-12-19
WO2006045936A3 (fr) 2006-06-22
FR2877103B1 (fr) 2012-02-10
EP1817620B1 (fr) 2012-09-26
FR2877103A1 (fr) 2006-04-28
EP1817620A2 (fr) 2007-08-15
CA2584748A1 (fr) 2006-05-04
CA2584748C (fr) 2015-10-13
US20070290145A1 (en) 2007-12-20
JP2008517291A (ja) 2008-05-22

Similar Documents

Publication Publication Date Title
ES2396426T3 (es) Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra
ES2347871T3 (es) Procedimiento y equipo de procesamiento de imágenes de fluorescencia de alta resolución por fibra óptica y particularmente de procesamiento de imágenes confocal.
ES2247427T3 (es) Aparato de formacion de imagenes confocal en particular para endoscopio.
US6879394B2 (en) Multi-photon imaging installation
ES2564529T3 (es) Dispositivo y procedimiento para la microscopía de fluorescencia multifotónica para la obtención de informaciones de tejidos biológicos
ES2402433T3 (es) Procedimiento y sistema de diagnóstico por imagen microscópica de fluorescencia de fibras de multimarcado
CN106037831B (zh) 实现增强信噪比的多光子光学成像的光纤光学装置
US20070213618A1 (en) Scanning fiber-optic nonlinear optical imaging and spectroscopy endoscope
US20110122488A1 (en) Multiple-photon excitation light sheet illumination microscope
US11237370B2 (en) Multiple inclined beam line-scanning imaging apparatus, methods, and applications
Wokosin et al. Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities
ES2647468T3 (es) Equipo de espectroscopia de autofluorescencia subsuperficial
US9155474B2 (en) System for multispectral imaging of fluorescence
US20040143190A1 (en) Mapping neural and muscular electrical activity
JP2003344777A (ja) 光ファイバ顕微鏡および内視鏡
JP2015006437A (ja) メチレンブルーに基づいた、ファイバーによる蛍光顕微鏡法
Ducourthial et al. Label free multiphoton imaging of human pulmonary tissues through two-meter-long microstructured fiber and multicore image-guide
VEILLEUX Towards All Optical Functional Imaging of Neuronal Cells
Weinigel et al. Clinical multiphoton endoscopy with FLIM capability
Veilleux Towards all optical functional imaging of neuronal cells: development of a video rate multimodal laser scanning microscope
Martín Civiac Confocal microendoscopy
Wang et al. MICROSCOPY/ONCOLOGY/IN-VIVO IMAGING: Miniature microscopes for guiding brain tumor resection Accurate, real-time delineation of brain tumor margins promises improved resection without debilitating effects. Optical sectioning technologies are now being developed for this purpose; other approaches show promise, too.