ES2375860B8 - Método para la obtención de células precursoras de oligodendrocitos. - Google Patents
Método para la obtención de células precursoras de oligodendrocitos. Download PDFInfo
- Publication number
- ES2375860B8 ES2375860B8 ES201030493A ES201030493A ES2375860B8 ES 2375860 B8 ES2375860 B8 ES 2375860B8 ES 201030493 A ES201030493 A ES 201030493A ES 201030493 A ES201030493 A ES 201030493A ES 2375860 B8 ES2375860 B8 ES 2375860B8
- Authority
- ES
- Spain
- Prior art keywords
- obtaining
- precursor cells
- pos
- oligodendrocit
- cerebral cortex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 4
- 239000002243 precursor Substances 0.000 title 1
- 210000003710 cerebral cortex Anatomy 0.000 abstract 2
- 210000000535 oligodendrocyte precursor cell Anatomy 0.000 abstract 2
- 241000124008 Mammalia Species 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 210000004556 brain Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000024245 cell differentiation Effects 0.000 abstract 1
- 230000005012 migration Effects 0.000 abstract 1
- 238000013508 migration Methods 0.000 abstract 1
- 230000037230 mobility Effects 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0623—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
- C12N2533/32—Polylysine, polyornithine
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Método para la obtención de células precursoras de oligodendrocitos.#Método para la purificación de células precursoras de oligodendrocitos (POs) de corteza cerebral a partir de la corteza cerebral de un mamífero de edad superior a una semana, que comprende: obtener una muestra aislada de cerebro; seleccionar las POs de la muestra biológica obtenida anteriormente y cultivar las POs en condiciones de proliferación, migración, movilidad o diferenciación celular. Además, la presente invención se refiere a los Pos obtenidos por el método de la invención y a las líneas celulares derivadas del mismo.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201030493A ES2375860B8 (es) | 2010-03-31 | 2010-03-31 | Método para la obtención de células precursoras de oligodendrocitos. |
| EP11721797A EP2554663A2 (en) | 2010-03-31 | 2011-03-22 | Method for obtaining oligodendrocyte precursor cells |
| PCT/ES2011/070191 WO2011121158A2 (es) | 2010-03-31 | 2011-03-22 | Método para la obtención de células precursoras de oligodendrocitos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201030493A ES2375860B8 (es) | 2010-03-31 | 2010-03-31 | Método para la obtención de células precursoras de oligodendrocitos. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2375860A1 ES2375860A1 (es) | 2012-03-07 |
| ES2375860B1 ES2375860B1 (es) | 2013-01-25 |
| ES2375860B8 true ES2375860B8 (es) | 2013-06-10 |
Family
ID=44484795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201030493A Expired - Fee Related ES2375860B8 (es) | 2010-03-31 | 2010-03-31 | Método para la obtención de células precursoras de oligodendrocitos. |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP2554663A2 (es) |
| ES (1) | ES2375860B8 (es) |
| WO (1) | WO2011121158A2 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115340982A (zh) * | 2022-08-23 | 2022-11-15 | 徐州医科大学 | 一种分离并纯化新生小鼠皮质区少突胶质前体细胞的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7152296A (en) | 1995-08-16 | 1997-03-12 | Board Of Trustees Of The Leland Stanford Junior University | Adult oligodendrocyte precursor cell compositions and methods |
| AR046076A1 (es) * | 2003-07-18 | 2005-11-23 | Otsuka Pharma Co Ltd | Procedimiento para obtener una poblacion homogenea de celulas precursoras de oligodendrocitos y poblacion obtenida |
| EP1684706A4 (en) * | 2003-11-10 | 2008-01-09 | Cornell Res Foundation Inc | DIFFERENT EXPRESSED GENES THROUGH ACUTE INSULATED PRESERVED CELLS OF DURA MATERIAL OF MAN |
| US20060183147A1 (en) * | 2005-02-11 | 2006-08-17 | Elan Pharmaceuticals, Inc. | Methods of identifying genes which modulate myelination |
-
2010
- 2010-03-31 ES ES201030493A patent/ES2375860B8/es not_active Expired - Fee Related
-
2011
- 2011-03-22 WO PCT/ES2011/070191 patent/WO2011121158A2/es active Application Filing
- 2011-03-22 EP EP11721797A patent/EP2554663A2/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2554663A2 (en) | 2013-02-06 |
| ES2375860B1 (es) | 2013-01-25 |
| WO2011121158A3 (es) | 2011-12-08 |
| WO2011121158A2 (es) | 2011-10-06 |
| ES2375860A1 (es) | 2012-03-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 2375860 Country of ref document: ES Kind code of ref document: B1 Effective date: 20130125 |
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| FD2A | Announcement of lapse in spain |
Effective date: 20210915 |