ES2331292B1 - PROCEDURE FOR THE PREPARATION OF A PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPOD. - Google Patents
PROCEDURE FOR THE PREPARATION OF A PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPOD.Info
- Publication number
- ES2331292B1 ES2331292B1 ES200850027A ES200850027A ES2331292B1 ES 2331292 B1 ES2331292 B1 ES 2331292B1 ES 200850027 A ES200850027 A ES 200850027A ES 200850027 A ES200850027 A ES 200850027A ES 2331292 B1 ES2331292 B1 ES 2331292B1
- Authority
- ES
- Spain
- Prior art keywords
- muscle
- cephalopod
- procedure
- protein
- myofibrillar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 27
- 210000003205 muscle Anatomy 0.000 title claims abstract description 18
- 241000238366 Cephalopoda Species 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000012141 concentrate Substances 0.000 title claims abstract description 10
- 230000007928 solubilization Effects 0.000 claims abstract description 6
- 238000005063 solubilization Methods 0.000 claims abstract description 6
- 230000002378 acidificating effect Effects 0.000 claims abstract description 5
- 238000001556 precipitation Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000001413 cellular effect Effects 0.000 claims description 4
- 239000002577 cryoprotective agent Substances 0.000 claims description 3
- 210000003195 fascia Anatomy 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000047 product Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000019832 sodium triphosphate Nutrition 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procedimiento de elaboración de un concentrado proteico a partir de músculo de cefalópodo que comprende una etapa de solubilización de las proteínas miofibrilares a pH ácidos.Procedure for making a concentrate protein from cephalopod muscle comprising a stage of solubilization of myofibrillar proteins at acidic pH.
Description
Procedimiento de elaboración de un concentrado proteico a partir de músculo de cefalópodo.Procedure for making a concentrate protein from cephalopod muscle.
La presente invención se refiere a un procedimiento de elaboración de un concentrado proteico a partir de músculo de cefalópodo.The present invention relates to a procedure for making a protein concentrate from cephalopod muscle
Son conocidos diversos procedimientos para la obtención de un concentrado proteico a partir del músculo de cefalópodo.Various procedures are known for obtaining a protein concentrate from the muscle of cephalopod
El músculo de cefalópodo es muy adecuado para la obtención de concentrados proteicos, ya que este músculo tiene poco color, poco sabor, ausencia de grasa y existen grandes recursos de él en la naturaleza.The cephalopod muscle is very suitable for obtaining protein concentrates, since this muscle has little color, little flavor, absence of fat and there are great resources of him in nature.
A partir de estos concentrados proteicos, la industria de la alimentación puede obtener un producto final que sea sucedáneo de pescado.From these protein concentrates, the food industry can get a final product that It is a substitute for fish.
En el documento con número de publicación ES2208105 se describe un procedimiento de elaboración de un concentrado proteico obtenido por la precipitación isoeléctrica de las proteínas miofibrilares a un pH alrededor de 5.In the document with publication number ES2208105 describes a procedure for preparing a protein concentrate obtained by isoelectric precipitation of Myofibrillar proteins at a pH around 5.
La presente invención describe un procedimiento de elaboración de un concentrado proteico a partir de músculo de cefalópodo.The present invention describes a process of making a protein concentrate from muscle cephalopod
El producto obtenido por este procedimiento es un producto intermedio de elaboración. A partir de él y tratado convenientemente es la base de elaboración de diferentes productos y sucedáneos.The product obtained by this procedure is an intermediate processing product. From him and tried conveniently it is the basis of elaboration of different products and substitutes.
El producto que es obtenido por este procedimiento está constituido por agua 70-80%, proteínas miofibrilares de cefalópodos (10-20%) y crioprotectores hasta completar el 100% en caso de conservarse en estado congelado.The product that is obtained by this procedure consists of 70-80% water, myofibrillar proteins of cephalopods (10-20%) and cryoprotectants to complete 100% if stored in been frozen
El producto obtenido tiene una alta capacidad de gelificación, esta característica es importante cuando en procesos posteriores se mezcla con cloruro sódico o potásico, y se añade determinados ingredientes y aditivos, y se somete a calentamiento o a altas presiones.The product obtained has a high capacity of gelation, this feature is important when in processes Subsequently mixed with sodium or potassium chloride, and added certain ingredients and additives, and undergoes heating or at high pressures
El procedimiento comprende al menos los siguientes pasos:The procedure comprises at least the Next steps:
\bullet Eliminación de la túnica externa e interna del cefalópodo.Removal of the external tunic e Cephalopod internal.
\bullet Picado del músculo del cefalópodo, con objeto de que la solubilización de las proteínas sea completa.Cephalopod muscle chopped, in order that the solubilization of the proteins is complete.
\bullet Homogeinización del músculo.Muscle homogenization.
\bullet Filtración para eliminar cualquier resto de pieles y fascias que haya podido quedar en la solución músculo/agua.Filtration to remove any rest of skins and fascias that could have remained in the solution muscle / water
\bullet Solubilización de las proteínas miofibrilares a pH ácidosSolubilization of proteins myofibrillar acidic pH
\bullet Separación de las proteínas miofibrilares de componentes celulares y extracelulares por precipitación isoeléctrica.Protein separation myofibrillar of cellular and extracellular components by isoelectric precipitation
\bullet Recogida de del precipitado proteico.Collection of the precipitate protein
Los componentes celulares que quedan solubilizados en la esta etapa de precipitación isoeléctrica son sales, nucleótidos, fosfatos, aminoácidos, péptidos.The cellular components that remain solubilized in this stage of isoelectric precipitation are salts, nucleotides, phosphates, amino acids, peptides.
El procedimiento de la invención en este ejemplo de realización comprende las siguientes etapas:The process of the invention in this example of implementation includes the following stages:
Eliminación de la túnica externa e interna del cefalópodo.Removal of the external and internal tunic of the cephalopod
Picado del músculo.Chopped muscle.
Homogenización del músculo picado en agua en una proporción de por 1 parte de músculo por cada 1 a 10 partes de agua.Homogenization of the chopped muscle in water in a proportion of 1 part of muscle for every 1 to 10 parts of Water.
Eliminación de restos de fascias las pieles.Removal of remains of fascia skins.
Solubilización de las proteínas miofibrilares en medio ácido, para conseguir este pH se lleva a cabo la adición de agente ácido hasta alcanzar el pH requerido.Solubilization of myofibrillar proteins in acidic medium, to achieve this pH, the addition of acidic agent until the required pH is reached.
Separación de las proteínas miofibrilares de otros componentes celulares por precipitación isoeléctrica de las proteínas miofibrilares a un pH entre 4 y 7 mediante la adición de agente alcalino.Separation of myofibrillar proteins from other cellular components by isoelectric precipitation of the Myofibrillar proteins at a pH between 4 and 7 by adding alkaline agent
Recogida del precipitado proteico mediante centrifugación y/o filtración.Protein precipitate collection by centrifugation and / or filtration.
Ajuste de la proteína a pH neutro mediante la adición de bicarbonato sódico u otro agente alcalino.Adjustment of the protein to neutral pH by means of addition of sodium bicarbonate or other alkaline agent.
Se pueden añadir agentes crioprotectores por ejemplo 5% de sacarosa, 4% de sorbitol y 0,5% de tripolifosfato sódico para su conservación por congelación.Cryoprotectants can be added by example 5% sucrose, 4% sorbitol and 0.5% tripolyphosphate sodium for preservation by freezing.
No alteran la esencialidad de esta invención variaciones en materiales, forma, tamaño y disposición de los elementos componentes, descritos de manera no limitativa, bastando ésta para proceder a su reproducción por un experto.They do not alter the essentiality of this invention variations in materials, shape, size and arrangement of component elements, described in a non-limiting manner, sufficient this one to proceed to its reproduction by an expert.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200850027A ES2331292B1 (en) | 2006-08-30 | 2006-08-30 | PROCEDURE FOR THE PREPARATION OF A PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPOD. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200850027A ES2331292B1 (en) | 2006-08-30 | 2006-08-30 | PROCEDURE FOR THE PREPARATION OF A PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPOD. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2331292A1 ES2331292A1 (en) | 2009-12-28 |
| ES2331292B1 true ES2331292B1 (en) | 2010-10-18 |
Family
ID=41404680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200850027A Expired - Fee Related ES2331292B1 (en) | 2006-08-30 | 2006-08-30 | PROCEDURE FOR THE PREPARATION OF A PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPOD. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2331292B1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6451975B1 (en) * | 1996-12-21 | 2002-09-17 | Advanced Protein Technologies, Inc. | Protein composition and process for isolating a protein composition from a muscle source |
| US6005073A (en) * | 1996-12-21 | 1999-12-21 | Advanced Protein Technologies, Inc. | Process for isolating a protein composition from a muscle source and protein composition |
| ES2208105B1 (en) * | 2002-10-25 | 2005-10-01 | Consejo Sup. De Invest. Cientificas | PROCEDURE FOR THE PREPARATION OF A FUNCTIONAL PROTEIN CONCENTRATE FROM MUSCLE OF CEPHALOPODES AND PRODUCT SO OBTAINED. |
-
2006
- 2006-08-30 ES ES200850027A patent/ES2331292B1/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| KAHN, L. et al. Squid protein isolate: Effect of processing conditions on recovery yields. Journal of Food Science, 1974, vol. 39, páginas 592-595. * |
| KAHN, L.N. et al. Squid protein concentrates. I. Evaluation of processes and product characterisics. Lebensmittel- Wissenschaft und Technologie, 1975, vol. 8 (2), páginas 64-69. * |
| MAZA, S. y ROSALES, M. Procesamiento de surimi de "{}pota"{} (Dosidicus gigas) por solubilización ácida-alcalina y precipitación isoeléctrica. Bol. Investig. Ins. Tecn. Pesq. Perú. 2004, vol. 6, páginas 1-7. * |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2331292A1 (en) | 2009-12-28 |
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