JP2003144097A - Water-soluble processed meat product - Google Patents

Water-soluble processed meat product

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Publication number
JP2003144097A
JP2003144097A JP2002253813A JP2002253813A JP2003144097A JP 2003144097 A JP2003144097 A JP 2003144097A JP 2002253813 A JP2002253813 A JP 2002253813A JP 2002253813 A JP2002253813 A JP 2002253813A JP 2003144097 A JP2003144097 A JP 2003144097A
Authority
JP
Japan
Prior art keywords
water
meat
treatment
soluble
meat preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2002253813A
Other languages
Japanese (ja)
Other versions
JP4130560B2 (en
Inventor
Shinji Toki
真治 土岐
Yukiko Ito
友季子 伊藤
Hiroshi Ide
弘 井手
Akihito Hattori
昭仁 服部
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Society for Techno Innovation of Agriculture Forestry and Fisheries
NH Foods Ltd
Original Assignee
Nippon Meat Packers Inc
Society for Techno Innovation of Agriculture Forestry and Fisheries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Meat Packers Inc, Society for Techno Innovation of Agriculture Forestry and Fisheries filed Critical Nippon Meat Packers Inc
Priority to JP2002253813A priority Critical patent/JP4130560B2/en
Publication of JP2003144097A publication Critical patent/JP2003144097A/en
Application granted granted Critical
Publication of JP4130560B2 publication Critical patent/JP4130560B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

PROBLEM TO BE SOLVED: To provide a processed meat produce easily dissolving again in water. SOLUTION: The processed meat product is obtained by carrying out a preservability imparting process which improves stability of the solution, comprises heat processing of a water-soluble meat protein solution, addition for a sugar to the solution after removing aggregate by centrifugal separation, subjecting to an ultrasonic treatment, or adjusting pH to acidity or alkalinity. The product has the preserbability together with high solubility to water and can be used in various foods.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【発明の属する技術分野】本発明は、水可溶性食肉調製
品に関する。
TECHNICAL FIELD The present invention relates to a water-soluble meat preparation.

【0002】[0002]

【従来の技術】タンパク質は人類の生存に欠かすことの
できない栄養素である。また、大豆、小麦、魚肉、食
肉、鶏卵や牛乳等のタンパク質食品または該タンパク質
食品由来の調製品は、栄養源のみならず、保水性、ゲル
化性、粘性賦与性、起泡性、呈味性等の多くの加工特性
も有するので、食品工業分野で広く利用されている。
BACKGROUND OF THE INVENTION Proteins are essential nutrients for human survival. In addition, protein foods such as soybeans, wheat, fish meat, meat, eggs and milk, or preparations derived from the protein foods are not only a nutritional source but also water retention, gelling property, viscosity imparting property, foaming property, taste. Since it also has many processing characteristics such as properties, it is widely used in the food industry.

【0003】タンパク質食品のうち、動物性タンパク質
食品は植物性タンパク質食品と比較してヒトが体内で合
成できない必須アミノ酸を多く含むので、ヒトの身体に
とって効率のよい栄養源となる。
Among protein foods, animal protein foods contain a large amount of essential amino acids that humans cannot synthesize in the body as compared with vegetable protein foods, and thus they can be an efficient nutrition source for the human body.

【0004】動物性タンパク質食品のうち鶏卵、牛乳は
水に溶解するが、魚肉や食肉(以下、魚肉を含めて食肉
という)は水に溶解しない。食肉(筋肉)が水に容易に
溶解しないのは、(1)筋肉を構成する筋原線維は、水
に溶解せず、高濃度の食塩でなければ溶けない性質であ
り、(2)筋原線維は、ミオシン、アクチン、コネクチ
ン、α-アクチニン等の多種類のタンパク質が、複雑な
構造と相互作用を有する強固な構成単位(サルコメア)
の集合体として成り立っており、(3)筋原線維の数本
を構成単位とする筋繊維の外周を結締組織が取り巻き、
さらに、その数束を結締組織が取り巻くなど、高次構造
を成していること等に因る。
Among animal protein foods, chicken eggs and milk are soluble in water, but fish meat and meat (hereinafter, meat including fish meat) are not soluble in water. Meat (muscle) is not easily dissolved in water because (1) the myofibrils that make up the muscle are insoluble in water and cannot be dissolved unless high-concentration salt is used. (2) Myofibrils Fiber is a strong structural unit (sarcomere) in which various types of proteins such as myosin, actin, connectin, and α-actinin have complex structures and interactions.
And (3) the tightening tissue surrounds the outer periphery of the muscle fiber which is composed of several myofibrils as a constituent unit.
In addition, it is due to the fact that it has a higher-order structure, such as a fastening structure surrounding the bundle.

【0005】このため、たとえ、食肉をミキサーにか
け、ペースト状にしたとしても、水に分散させるとすぐ
に沈殿する。一方、食肉を酸やプロテアーゼ等の酵素を
用いて極低分子のペプチドやアミノ酸に加水分解する
と、水に溶解させることができる。なお、本発明では所
望の濃度以下で水に溶解、懸濁または分散させる時、容
易に溶解、懸濁または分散し、その状態を所望の期間維
持し、沈殿物を生じない状態を溶解という。
Therefore, even if the meat is put into a paste by mixing it with the meat, it is immediately precipitated when dispersed in water. On the other hand, when meat is hydrolyzed into very low molecular weight peptides or amino acids using an enzyme such as acid or protease, it can be dissolved in water. In the present invention, when dissolved, suspended or dispersed in water at a desired concentration or less, it is easily dissolved, suspended or dispersed, the state is maintained for a desired period, and a state in which no precipitate is formed is referred to as dissolution.

【0006】食肉の加水分解物は呈味性のアミノ酸や極
低分子のペプチドを多く含むので、調味料(HAP)とし
て利用されている。しかし、HAPは高分子物質である食
肉タンパク質が有している味や物性機能を発現すること
はできない。一方、HAPはアミノ酸や極低分子のペプチ
ドに起因する調味料的な味を呈するので、一度に多量を
摂取することはできない。言換えれば、実用的な見地か
らは、HAPは窒素栄養源としての価値は少ないまたは無
いと言える。
[0006] Since the hydrolyzate of meat contains a lot of tasty amino acids and very low molecular weight peptides, it is used as a seasoning (HAP). However, HAP cannot express the taste and physical properties of the high molecular weight meat protein. On the other hand, since HAP has a taste like a seasoning attributed to amino acids and extremely low-molecular peptides, it is not possible to ingest a large amount at one time. In other words, from a practical standpoint, HAP has little or no value as a nitrogen nutrient source.

【0007】食肉などの筋肉タンパク質食品は、比較的
高濃度の食塩水[0.3M以上、約1.8%(W/V)以上]中で
ホモジナイズすれば、一定濃度までは溶解させることが
できる。しかし、このように調製された調製物は食塩含
量が高いので、食味上の制約から、その用途は限られた
ものであった。
Muscle protein foods such as meat can be dissolved up to a certain concentration by homogenizing in a relatively high concentration saline solution [0.3M or more, about 1.8% (W / V) or more]. it can. However, since the thus-prepared preparation has a high salt content, its use was limited due to taste restrictions.

【0008】食肉を水または低濃度の食塩水で可溶化す
ることができれば、食肉をより広く利用することが可能
になる。また、HAP的な味を呈さず、魚肉や食肉の本格
的な味を呈し、さらに魚肉や食肉の保水性、ゲル化性、
粘性賦与性、起泡性等の本来的な加工適性を発現するの
で、食品工業分野への用途が広がるものと思われる。
If the meat can be solubilized with water or a low-concentration saline solution, the meat can be used more widely. In addition, it does not have a HAP-like taste, but it has a full-fledged taste of fish meat and meat.
Since it exhibits inherent processing suitability such as viscosity-imparting property and foaming property, it is expected that its application to the food industry field will be expanded.

【0009】これまでに、魚肉を水可溶化させる方法が
報告されている(J.Agric.Food.Chem.42,2646-2664(199
4))。また、食肉を水溶化させる方法が報告されている
(平成10年度食肉に関する助成研究調査成果報告書Vol.
17、301-306、(1999)伊藤記念財団発行)。その概要は
次の通りである。
A method for solubilizing fish meat in water has been reported so far (J. Agric. Food. Chem. 42, 2646-2664 (199).
Four)). In addition, a method of solubilizing meat has been reported (Fiscal 1998 Grant Research Report on Meat Vol.
17, 301-306, (1999) Published by Ito Memorial Foundation). The outline is as follows.

【0010】食肉(豚、牛、鶏等)を細切りし、ヒスチ
ジンでpH7.5に調整した25mM NaCl溶液[約0.15%(W/
V)]を10倍量加え、ホモジナイズする。18000G、20分
間遠心分離して沈殿を回収し、さらに25mM NaCl溶液を5
倍量加えホモジナイズし、遠心分離し、沈殿を回収す
る。この操作を、再度繰り返す。回収した沈殿に、ヒス
チジンでpH7.5に調整した2.5mM NaCl溶液[約0.01%(W/
V)]を5倍量加えホモジナイズし、遠心分離して沈殿を
回収する。
Meat (pork, beef, chicken, etc.) is shredded and adjusted to pH 7.5 with histidine to a 25 mM NaCl solution [about 0.15% (W /
V)] is added in an amount of 10 times and homogenized. Centrifuge at 18000 G for 20 minutes to collect the precipitate, and add 25 mM NaCl solution to 5
Add twice the amount, homogenize, centrifuge and collect the precipitate. This operation is repeated again. To the collected precipitate, a 2.5 mM NaCl solution adjusted to pH 7.5 with histidine [about 0.01% (W /
V)] is added in an amount of 5 times, homogenized, and centrifuged to collect the precipitate.

【0011】回収した沈殿を適当量(肉の15から20倍
量)の水に懸濁し、この懸濁液50ml当り5分間の超音波
処理を施した後、37000G、20分間遠心分離し、その上清
を回収して、水に溶解する食肉を調製する。なお、この
ようにして調製された水に溶解する食肉は、SDS-PAGE像
から、筋原線維を構成するタンパク質のうち、コネクチ
ンやα-アクチニンが減少または欠失していると報告さ
れている。
The recovered precipitate was suspended in an appropriate amount of water (15 to 20 times the amount of meat), sonicated for 5 minutes per 50 ml of this suspension, and then centrifuged at 37,000 G for 20 minutes. The supernatant is collected to prepare meat that dissolves in water. The thus-prepared water-soluble meat is reported to have reduced or deleted connectin and α-actinin among the proteins constituting myofibrils from the SDS-PAGE image. .

【0012】この方法は、非常に複雑で強固な構造体で
ある筋肉(食肉)を薄い塩溶液で洗浄し、最終的に水の
ような極低イオン強度の溶液に分散し、膨潤させ脆く
し、その後に超音波処理のような物理的な力を加えるこ
とにより、該構造体を破壊し、本来、水に溶解しない食
肉を水に溶けるようにさせていると考えられる。
In this method, muscle (meat), which is a very complex and strong structure, is washed with a thin salt solution, and finally dispersed in an extremely low ionic strength solution such as water to swell and make it brittle. After that, it is considered that the structure is destroyed by applying a physical force such as ultrasonic treatment so that the meat, which is originally insoluble in water, can be dissolved in water.

【0013】なお、上記の調製品をそのまま静置してお
くと、沈殿を生じるが、該調製品を70℃、10分間加熱処
理しておくと、所定時間静置後も沈殿は生じない。
When the above prepared product is allowed to stand as it is, precipitation occurs, but when the prepared product is heat-treated at 70 ° C. for 10 minutes, no precipitation occurs even after standing for a predetermined time.

【0014】[0014]

【発明が解決しようとする課題】上記の方法に従って得
られた水に可溶化された食肉は、水またはイオン強度の
低い食塩水に溶解しているので、そのままの状態では保
存に適さない。そこで、冷蔵、冷凍、加熱殺菌(パスチ
ュライゼーション)、高温加圧殺菌(レトルト殺菌)、
超高圧殺菌、微生物の芽胞及び/又は栄養細胞のリシス
を生じる程度の超音波殺菌、または乾燥(含む、熱風乾
燥、噴霧乾燥、凍結乾燥など)などの慣用の保存性を賦
与するための処理(以下、保存性賦与処理という)が施
される。
The water-solubilized meat obtained by the above method is not suitable for storage as it is because it is dissolved in water or saline having a low ionic strength. Therefore, refrigeration, freezing, heat sterilization (pasturization), high temperature pressure sterilization (retort sterilization),
Ultra-high pressure sterilization, ultrasonic sterilization to such an extent as to cause lysis of microbial spores and / or vegetative cells, or treatment for imparting conventional preservability such as drying (including hot air drying, spray drying, freeze drying, etc.) ( Hereinafter, the preservability imparting process) is performed.

【0015】しかし、水に溶解させた食肉に保存性賦与
処理を施した後、または該処理後一定の期間保存した
後、再び水に溶解させようとする時、溶解性(以下、再
溶解性という)は著しく低下し、沈殿を生じる、または
溶解しない。なお、再び水に溶解させようとした後に、
37000Gで遠心分離処理し、遠心分離処理前後のタンパク
質含量を測定すれば、その比から再溶解率を求めること
ができる。
However, when the meat dissolved in water is subjected to a preservability-imparting treatment, or after being stored for a certain period of time, and then re-dissolved in water, the solubility (hereinafter referred to as re-dissolvability) Is significantly reduced and causes precipitation or does not dissolve. In addition, after trying to dissolve in water again,
By centrifuging at 37000G and measuring the protein content before and after centrifugation, the redissolution rate can be determined from the ratio.

【0016】本発明は、水に溶解させた食肉に保存性賦
与処理を施すと再溶解性が著しく悪くなるという従来の
問題点を解決することを目的とするものであり、再溶解
性を保持するように調製した食肉調製品(以下、水溶性
食肉調製品という)を産業的に利用可能とすることを課
題とする。
The present invention is intended to solve the conventional problem that the re-solubility is remarkably deteriorated when a preservative-imparting treatment is applied to meat dissolved in water, and the re-solubility is maintained. It is an object of the present invention to make a prepared meat product (hereinafter referred to as a water-soluble prepared meat product) industrially usable.

【0017】[0017]

【課題を解決するための手段】本発明は上記課題を解決
するために、水に可溶化させた食肉に保存性賦与処理し
て得られる水可溶性食肉調製品であって、保存性賦与処
理に先立ち糖が添加されていることにより水に対し高い
再溶解性を有することを特徴とする水可溶性食肉調製品
を提供する。
In order to solve the above-mentioned problems, the present invention is a water-soluble meat preparation obtained by preserving solubilized meat in water to give it a preservability treatment. Disclosed is a water-soluble meat preparation characterized by having high re-solubility in water due to the addition of sugar in advance.

【0018】本発明は上記課題を解決するために、水に
溶解させた食肉に保存性賦与処理して得られる水溶性食
肉調製品であって、保存性賦与処理に先立ち加熱処理及
び糖添加処理を行うことにより、水に対し高い再溶解性
を有することを特徴とする水可溶性食肉調製品を提供す
る。
In order to solve the above-mentioned problems, the present invention is a water-soluble meat preparation obtained by preserving storability of meat dissolved in water, which comprises heat treatment and sugar addition treatment prior to preserving treatment. By performing the above, a water-soluble meat preparation product characterized by having a high re-solubility in water is provided.

【0019】本発明は上記課題を解決するために、水に
溶解させた食肉に保存性賦与処理して得られる水溶性食
肉調製品であって、保存性賦与処理に先立ち超音波処理
を行うことにより、水に対し高い再溶解性を有すること
を特徴とする水可溶性食肉調製品を提供する。
In order to solve the above-mentioned problems, the present invention is a water-soluble meat preparation obtained by preserving storability of meat dissolved in water, wherein ultrasonic treatment is performed prior to preserving treatment. Provides a water-soluble meat preparation characterized by having a high re-solubility in water.

【0020】本発明は上記課題を解決するために、水に
溶解させた食肉に保存性賦与処理して得られる水溶性食
肉調製品であって、保存性賦与処理に先立ち、調整品の
pHを非中性域、具体的には酸性域ではpH5以下、ア
ルカリ性域ではpH7.5以上にする処理(以下、「非
中性化処理」と呼ぶ。)を行うことにより、水に対し高
い再溶解性を有することを特徴とする水可溶性食肉調製
品を提供する。なお、高い再溶解性とは、20℃の水に対
する再溶解率が30%以上、より好ましくは50%以上、も
っとも好ましくは70%以上である状態をいう。
In order to solve the above-mentioned problems, the present invention is a water-soluble meat preparation obtained by preserving storability of meat dissolved in water, wherein the pH of the adjusted product is adjusted prior to preserving treatment. High redissolvability in water by performing treatment to adjust pH to 5 or less in non-neutral region, specifically acidic region, and pH 7.5 or more in alkaline region (hereinafter referred to as "non-neutralizing treatment") Disclosed is a water-soluble meat preparation characterized by having a property. The term “high redissolvability” means that the redissolution rate in water at 20 ° C. is 30% or more, more preferably 50% or more, and most preferably 70% or more.

【0021】本発明の水溶性食肉調製品は健常人、咀嚼
機能や嚥下機能の弱い乳幼児、高齢者又は咀嚼機能に障
害を持った人や病人用等の多種の食品及び食品の素材と
して利用することができる。流動食、ゲル状食品、栄養
補助食品、とろみ調整剤、錠剤、ビスケット、スープ
類、ドリンク類、スポーツ飲料、またはスポーツ食品な
ど及びそれら食品の素材として利用する事ができる。
The water-soluble meat preparation of the present invention is used as a variety of foods and food materials for healthy people, infants with weak chewing function and swallowing function, elderly people, people with impaired chewing function and sick people. be able to. It can be used as a liquid food, a gel food, a nutritional supplement, a thickening agent, tablets, biscuits, soups, drinks, sports drinks, sports foods and the like, and materials for these foods.

【0022】上記保存性賦与処理に先立つ上記超音波処
理は、上記食肉の筋束、筋繊維若しくは筋原線維から成
る構造体、又は該構造体を構成するタンパク質群の分子
構造を、弛緩した状態又は低分子化した状態にすること
のできる仕事量の超音波処理であることで特徴づけられ
る。
The ultrasonic treatment prior to the preservation-imparting treatment is carried out by relaxing the molecular structure of the muscle bundles of muscle, muscle fibers or myofibrils, or the protein group constituting the structure. Alternatively, it is characterized in that it is an ultrasonic treatment with a work amount capable of reducing the molecular weight.

【0023】上記保存性賦与処理に先立つ上記超音波処
理は、上記食肉の筋原線維を構成するタンパク質である
ミオシンの低分子化を生じさせることのできる仕事量の
超音波処理であることで特徴づけられる。
The above-mentioned ultrasonic treatment prior to the preservation-imparting treatment is characterized in that the ultrasonic treatment has a work amount capable of causing a reduction in the molecular weight of myosin which is a protein constituting the myofibril of the meat. Be attached.

【0024】[0024]

【発明の実施の形態】本発明に係る水可溶性食肉調製
品、その製造方法、及び該調製品を利用した食品の実施
の形態を、実施例とともに以下説明する。本発明の水可
溶性食肉調製品が、容易に再溶解する食肉調製品とする
ために、本発明者等は、鋭意研究を進めた。
BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of a water-soluble meat preparation according to the present invention, a method for producing the same, and a food using the preparation will be described below together with Examples. In order to make the water-soluble meat preparation of the present invention into a meat preparation that can be easily redissolved, the present inventors have conducted intensive research.

【0025】上述のとおり、水に溶解させた食肉に保存
性賦与処理を施した後、または該処理後一定の期間保存
した後、再び水に溶解させようとする時、再溶解性は著
しく低下し沈殿を生じる、または溶解しない。
As described above, when the meat dissolved in water is subjected to a preservative-imparting treatment, or after being stored for a certain period of time after it is re-dissolved in water, the re-solubility is remarkably reduced. Precipitates or does not dissolve.

【0026】本発明者等は、再溶解性を高く維持するた
めに、次の水可溶性食肉調製品を発明するに至った。 1.水可溶性食肉調製品の保存性賦与処理を行う前に糖
を添加する。各種の糖類がタンパク質の変性を防止する
ことは広く知られており、例えば、魚肉スリ身を凍結保
存する場合には、凍結変性を防止するためにソルビトー
ル等が添加されている。これと同様に糖類の添加が水可
溶性食肉調製品の再溶解性の改善をもたらすか否かを検
討した。
The present inventors have invented the following water-soluble meat preparation in order to maintain high re-solubility. 1. Sugar is added before the preservation-imparting treatment of the water-soluble meat preparation. It is widely known that various sugars prevent protein denaturation. For example, in the case of cryopreserving fish meat, sorbitol or the like is added to prevent freezing denaturation. Similarly, it was examined whether addition of sugars would improve re-solubility of water-soluble meat preparations.

【0027】具体的には、細切りした食肉を中性域の低
濃度の塩溶液で洗浄し、超音波処理を行うことにより水
に溶解する食肉を調製する。そして、(1)水に溶解す
る食肉に糖を添加する、または、(2)水に溶解する食
肉を加熱処理し、加熱によって生じる少量の凝集物を除
去した後に糖を添加する。更に、(1)または(2)の
後に、保存性賦与処理を行って水可溶性食肉調製品を得
る。このようにして得られた水可溶性食肉調製品は、後
述するように、水に対し高い再溶解性を持つことが確認
された。
Specifically, the minced meat is washed with a low-concentration salt solution in the neutral range and subjected to ultrasonic treatment to prepare meat that dissolves in water. Then, (1) the sugar is added to the meat that dissolves in water, or (2) the meat that dissolves in water is heat-treated to remove a small amount of aggregates generated by heating, and then the sugar is added. Further, after (1) or (2), a preservative imparting treatment is performed to obtain a water-soluble meat preparation. The water-soluble meat preparation thus obtained was confirmed to have high resolubility in water, as described later.

【0028】2.水可溶性食肉調製品の保存性賦与処理
を行う前に、筋束、筋繊維または筋原線維、あるいはこ
れら構造体を構成するタンパク質群の分子構造をより弛
緩(loose)した状態、または、低分子化した状態にす
ることが再溶解性の改善をもたらすか否かを検討した。
そのためには、前記報告書で報告された超音波処理より
大きな仕事量の超音波処理を必要とする。この超音波処
理の強さは、既報の超音波処理では筋原線維を構成する
主要なタンパク質であるミオシンの低分子化を生じさせ
なかったことに対して、後述するように、ミオシンの低
分子化を生じることで特徴付けられる。このようにして
得られた水溶性食肉調製品は、後述するように、高い再
溶解性を持つことが確認された。
2. Before the preservation-imparting treatment of the water-soluble meat preparation, the molecular structure of muscle bundles, muscle fibers or myofibrils, or the proteins that compose these structures is loosened or low molecular weight. It was examined whether or not the changed state brings about improvement in resolubility.
For that purpose, ultrasonic treatment with a larger work amount than the ultrasonic treatment reported in the above report is required. The strength of this sonication did not cause the degradation of myosin, which is a major protein that constitutes myofibrils, by the previously reported sonication. It is characterized by causing The water-soluble meat preparation thus obtained was confirmed to have high re-dissolvability as described later.

【0029】具体的には、細切りした食肉を中性域の低
濃度の塩溶液で洗浄後、上記のような特徴を有するより
強い超音波処理を行うことにより水に溶解する食肉を調
製する。その後に保存性賦与処理を行う。このようにし
て得られた水可溶性食肉調製品は、水に対し高い再溶解
性を持つことが確認された。
Specifically, after the minced meat is washed with a low-concentration salt solution in the neutral range, it is subjected to stronger ultrasonic treatment having the above characteristics to prepare water-soluble meat. After that, the preservation property imparting process is performed. It was confirmed that the water-soluble meat preparation thus obtained has a high resolubility in water.

【0030】3.水可溶性食肉調製品の保存性賦与処理
を行う前に、筋束、筋繊維もしくは筋原線維、又はこれ
ら構造体を構成するタンパク質群の溶液のpHをこれら
のタンパク質群の等電点より離すことにより、再溶解性
の改善をもたらすか否かを検討した。ここで等電点と
は、タンパク質分子の総電荷が0となるpH値のこと
で、等電点においてタンパク質の溶解度が最も低下す
る。
3. Before performing the preservation-imparting treatment of water-soluble meat preparations, separate the pH of the solution of the muscle bundles, muscle fibers or myofibrils, or the proteins that compose these structures from the isoelectric point of these proteins. It was investigated whether or not to bring about improvement in resolubility. Here, the isoelectric point is a pH value at which the total charge of protein molecules is 0, and the solubility of the protein is the lowest at the isoelectric point.

【0031】筋原繊維を形成するアクチンとミオシンの
等電点は5付近、α−アクチニンの等電点は6付近であ
ることを踏まえ、筋原繊維全体としての最溶解性への影
響を見るために溶液のpHを酸性からアルカリ性までの
いくつかのpH値に変えて再可溶性を見たところ、酸性
ではpH5以下、アルカリ性ではpH7.5以上で高い
再可溶性が見出せた。このようにして非中性化処理で得
られた水溶性食肉調製品は、後述するように、高い再溶
解性を持つことが確認された。
Based on the fact that the isoelectric points of actin and myosin forming myofibrils are around 5, and the isoelectric point of α-actinin is around 6, the effect on the maximum solubility of myofibrils as a whole is examined. Therefore, when the pH of the solution was changed to several pH values from acidic to alkaline and re-solubilization was performed, high re-solubility was found at pH 5 or less when acidic and at pH 7.5 or more when alkaline. It was confirmed that the water-soluble meat preparation thus obtained by the non-neutralizing treatment has a high resolubility, as described later.

【0032】更に、このようなpH値を採用することに
より、細切りした食肉を中性域の低濃度の塩溶液で洗浄
後、超音波処理を行わずとも非中性化処理だけで、もし
くは超音波処理と非中性化処理を組み合わせて、水可溶
性食肉調製品を得られることを新たに見出した。
Further, by adopting such a pH value, after the minced meat is washed with a salt solution having a low concentration in the neutral range, it can be treated only with non-neutralizing treatment without ultrasonic treatment, or with super treatment. It was newly found that a water-soluble meat preparation can be obtained by combining sonication and non-neutralization.

【0033】具体的には、細切りした食肉を中性域の低
濃度の塩溶液で洗浄後、超音波処理を行わずに、上記の
ような非中性化処理を行うことにより、もしくは超音波
処理と非中性化処理を組み合わせて行うことにより、水
に溶解する食肉を調製する。その後に保存性賦与処理を
行う。このようにして得られた水可溶性食肉調製品は、
水に対し高い再溶解性を持つことが確認された。
Specifically, the minced meat is washed with a low-concentration salt solution in the neutral range and then subjected to the above-mentioned non-neutralizing treatment without ultrasonic treatment, or by ultrasonication. By combining the treatment and the non-neutralizing treatment, meat that dissolves in water is prepared. After that, the preservation property imparting process is performed. The water-soluble meat preparation thus obtained is
It was confirmed to have high resolubility in water.

【0034】以下、原料肉から水に溶解する食肉を製造
し、さらに本発明の水可溶性食肉調製品を製造する一連
の製造工程を説明する。
Hereinafter, a series of production steps for producing meat that dissolves in water from raw meat and further producing the water-soluble meat preparation of the present invention will be described.

【0035】(1)本発明に用いる原料は、食肉の種類
を問わないが、脂肪分の少ない部位が望ましい。食肉
(イワシ、マグロ、サケ、タラ等の魚類、タコ、イカ等
の軟体動物、ウシ、ブタ、ヒツジ等の哺乳類、ニワト
リ、ダチョウ等の鳥類、クロコダイル、ヘビ等の爬虫
類、カエル等の両生類の筋肉または内臓に由来する肉)
を細切りし、これにpH7.5に調整した低濃度の食塩水を
加え、撹拌、粉砕してホモジナイズする。ホモジナイズ
した食肉を遠心分離し、その沈殿を回収する。
(1) The raw material used in the present invention is not limited to any kind of meat, but is preferably a portion with a low fat content. Meat (fish such as sardines, tuna, salmon and cod, molluscs such as octopus and squid, mammals such as cows, pigs and sheep, birds such as chicken and ostrich, reptiles such as crocodile and snakes, and amphibian muscles such as frogs) Or meat derived from internal organs)
Slice into small pieces, add low-concentration saline adjusted to pH 7.5 to this, stir, pulverize, and homogenize. Centrifuge the homogenized meat and collect the precipitate.

【0036】(2)回収した沈殿に水を加え混合、撹拌
した後に超音波ホモジナイザーを用いて超音波処理もし
くは非中性化処理、又はこの両者を組み合わせた処理を
行った後、遠心分離して溶解していない不純物を取り除
き、水に溶解する食肉の溶液を得る。
(2) Water is added to the recovered precipitate, mixed and stirred, and then subjected to ultrasonic treatment or non-neutralization treatment using an ultrasonic homogenizer, or a treatment in which both are combined, followed by centrifugation. Undissolved impurities are removed to obtain a solution of meat that dissolves in water.

【0037】(3)この溶液を70℃にて加熱処理し、再
度遠心分離し、凝集物を除去して得られた溶液に糖(単
糖〜多糖類)を添加し、溶解させ、そのまま、もしく
は、逆浸透膜やゲルろ過膜等により濃縮後、冷蔵、冷
凍、加熱殺菌、高温加圧殺菌、超高圧殺菌、超音波殺菌
または乾燥等の保存性賦与処理を行う。これにより再溶
解性を有する水溶性食肉調製品を得る。
(3) This solution was heat-treated at 70 ° C., centrifuged again to remove aggregates, sugars (monosaccharides to polysaccharides) were added to the resulting solution, and the mixture was dissolved. Alternatively, after concentrating with a reverse osmosis membrane, a gel filtration membrane or the like, a preservation imparting treatment such as refrigeration, freezing, heat sterilization, high temperature pressure sterilization, ultra high pressure sterilization, ultrasonic sterilization or drying is performed. This gives a water-soluble meat preparation having resolubility.

【0038】(4)上記製造工程(3)の代わりに、水
に溶解する食肉の溶液に更なる超音波処理を行い、その
まま、もしくは、逆浸透膜やゲルろ過膜等により濃縮
後、冷蔵、冷凍、加熱殺菌、高温加圧殺菌、超高圧殺
菌、超音波殺菌または乾燥等の保存性賦与処理を行な
う。これにより再溶解性を有する水溶性食肉調製品を得
る。
(4) In place of the above production step (3), a solution of meat that dissolves in water is further subjected to ultrasonic treatment, and as it is or after being concentrated by a reverse osmosis membrane or a gel filtration membrane, refrigerated, Preservation imparting treatment such as freezing, heat sterilization, high temperature pressure sterilization, ultra high pressure sterilization, ultrasonic sterilization or drying is performed. This gives a water-soluble meat preparation having resolubility.

【0039】(5)上記製造工程(3)の代わりに、水
に溶解する食肉の溶液にクエン酸、乳酸、酢酸等の食用
可な有機酸、又はリン酸三ナトリウム、ピロリン酸ナト
リウム、トリポリリン酸ナトリウム等の食用可なアルカ
リ性塩を用いて非中性化処理を行い、そのまま、又は逆
浸透膜やゲルろ過膜等により濃縮後、冷蔵、冷凍、加熱
殺菌、高温加圧殺菌、超高圧殺菌、超音波殺菌もしくは
乾燥等の保存性賦与処理を行なう。これにより再溶解を
有する水溶性食肉調製品を得る。
(5) In place of the above production step (3), an edible organic acid such as citric acid, lactic acid, acetic acid, or trisodium phosphate, sodium pyrophosphate, tripolyphosphate is added to a solution of meat that dissolves in water. Non-neutralizing treatment using edible alkaline salts such as sodium, as it is, or after concentration by reverse osmosis membrane or gel filtration membrane, refrigeration, freezing, heat sterilization, high temperature pressure sterilization, ultra high pressure sterilization, Preservation treatment such as ultrasonic sterilization or drying is performed. This gives a water-soluble meat preparation with re-dissolution.

【0040】但し、上記製造工程(2)において既に非
中性化処理を行っていれば、上記製造工程(2)の後
に、そのまま、又は逆浸透膜やゲルろ過膜等により濃縮
後、冷蔵、冷凍、加熱殺菌、高温加圧殺菌、超高圧殺
菌、超音波殺菌もしくは乾燥等の保存性賦与処理を行な
う。これにより、同様に、再溶解性を有する水溶性食肉
調製品を得る。
However, if the non-neutralizing treatment has already been carried out in the production step (2), after the production step (2), as it is or after concentration with a reverse osmosis membrane or gel filtration membrane, refrigeration, Preservation imparting treatment such as freezing, heat sterilization, high temperature pressure sterilization, ultra high pressure sterilization, ultrasonic sterilization or drying is performed. This likewise gives a water-soluble meat preparation with resolubility.

【0041】このような製造工程により得られた水溶性
食肉調製品は、水に容易に再溶解する性質を有する。こ
の性質により、これまで利用範囲の限られていた食肉の
新たな利用形態を考えることができる。
The water-soluble meat preparation obtained by such a manufacturing process has a property of being easily redissolved in water. Due to this property, it is possible to consider a new form of use of meat, which has been limited in use until now.

【0042】たとえば、咀嚼機能や嚥下機能の弱い乳幼
児、高齢者又は咀嚼機能に障害を持った人や病人にも摂
取可能な流動食やゲル状食品、食肉タンパク質を豊富に
含む栄養補助食品、とろみ調整剤、錠剤、ビスケット、
スープ類又はドリンク類、栄養補助食品、スポーツフー
ド、非常食、アウトドア用携帯食等、その他いろいろな
種類の食品に応用できる。
[0042] For example, liquid foods, gel foods, dietary supplements rich in meat protein, and thickened foods that can be taken by infants, elderly people who have weak chewing and swallowing functions, elderly people, and people with impaired chewing function and sick people. Regulators, tablets, biscuits,
It can be applied to various kinds of foods such as soups or drinks, dietary supplements, sports foods, emergency foods, portable foods for outdoor use and the like.

【0043】さらに、食肉由来のタンパク質なので、従
来の畜産加工品への添加剤として利用すれば、食品の風
味を損なうことなく固形分付与、歩留まり改善、食感の
改良などの効果を発揮する。
Further, since it is a protein derived from meat, if it is used as an additive to a conventional processed animal product, it exerts effects such as imparting solid content, improving yield and improving texture, without impairing the flavor of food.

【0044】なお、上記製造工程(2)において、超音
波処理によって得られた水に溶解する食肉の溶液そのも
のに保存性賦与処理を施した場合には沈殿を生じ再可溶
化させることができなかった。即ち、該溶液を凍結し、
-18℃一ヶ月間保存後、解凍した時、沈殿を生じた。ま
た、該溶液をレトルトパウチに封入し、F0=3の条件でレ
トルト殺菌した時、沈殿を生じた。また該溶液を凍結乾
燥もしくは噴霧乾燥もしくは熱風乾燥して得られた乾燥
粉末を水に溶解させたが、ほとんど溶解しなかった(再
可溶化率は、10〜20%程度)。
In the production step (2), when the solution of meat which is dissolved in water and obtained by ultrasonic treatment is subjected to a preservability-imparting treatment, precipitation is caused and resolubilization cannot be performed. It was That is, freezing the solution,
After storing at -18 ° C for 1 month, when it was thawed, a precipitate was formed. Further, when the solution was sealed in a retort pouch and sterilized by retort under the condition of F 0 = 3, a precipitate was generated. A dry powder obtained by freeze-drying, spray-drying, or hot-air drying the solution was dissolved in water, but it was hardly dissolved (resolubilization rate is about 10 to 20%).

【0045】又、上記製造工程(2)において、超音波
処理によって得られた水に溶解する食肉の溶液を、70
℃、10分間加熱処理し、生成した凝集物を遠心分離によ
り取り除いた溶液そのものに保存性賦与処理を施した場
合には沈殿を生じ再可溶化させることができなかった。
即ち、該溶液を凍結し、-18℃一ヶ月間保存後、解凍し
た時、沈殿を生じた。また、該溶液をレトルトパウチに
封入し、F0=3の条件でレトルト殺菌した時、沈殿を生じ
た。また該溶液を凍結乾燥もしくは噴霧乾燥もしくは熱
風乾燥して得られた乾燥粉末についても、非加熱の場合
と同様に、水にほとんど溶解しなかった。
In the production step (2), the solution of meat dissolved in water obtained by ultrasonic treatment is
When the solution itself, which had been subjected to heat treatment at 10 ° C. for 10 minutes and the aggregates formed were removed by centrifugation, was subjected to a preservation-imparting treatment, precipitation was caused and resolubilization was not possible.
That is, when the solution was frozen, stored at -18 ° C for one month and then thawed, a precipitate was formed. Further, when the solution was sealed in a retort pouch and sterilized by retort under the condition of F 0 = 3, a precipitate was generated. Also, the dry powder obtained by freeze-drying, spray-drying or hot-air drying the solution was hardly dissolved in water as in the case of non-heating.

【0046】(実施例)以下、実施例により、再可溶化
が可能な乾燥粉末の調製条件と、その性質を具体的に説
明する。但し、本発明は、下記の実施例に限定されるも
のではない。
(Examples) Hereinafter, the conditions for preparing re-solubilized dry powders and their properties will be specifically described with reference to Examples. However, the present invention is not limited to the following examples.

【0047】A.乾燥処理後の再溶解率に及ぼす加熱処
理と糖添加の影響、並びに乾燥前の凍結保存条件による
影響 表1は、乾燥後の再可溶化率に及ぼす加熱処理と糖添加
の影響を実験した結果を示し、そして表2は、その乾燥
物を調製する場合の乾燥処理前の凍結保存条件による影
響を実験した結果を示す。以下この影響を特に、(a)
加熱処理をせず、糖を添加した場合(非加熱、糖添加)
と、(b)加熱処理を施し、糖を添加した場合(加熱、
糖添加)と、について夫々説明する。
A. Effect of heat treatment and addition of sugar on re-dissolution rate after drying treatment, and effect of freeze storage condition before drying Table 1 shows the results of experiments on the effect of heat treatment and addition of sugar on re-solubilization rate after drying. And Table 2 shows the results of experiments on the influence of the cryopreservation conditions before the drying treatment when preparing the dried product. This effect will be described in the following (a)
When sugar is added without heat treatment (non-heated, sugar added)
And (b) when heat treatment is performed and sugar is added (heating,
Addition of sugar) and) are described respectively.

【0048】(a)加熱処理をせず、糖を添加した場合
(非加熱、糖添加) 超音波処理によって得られた溶液に約1%濃度となる量
のトレハロースを添加し、凍結乾燥もしくは噴霧乾燥も
しくは熱風乾燥して得られた乾燥粉末は、60〜70%の再
溶解率を示した(表1:非加熱、糖添加)。そして、こ
の乾燥物を調製する場合、凍結保存の期間が長いほど、
得られた乾燥物の再溶解率は悪くなる傾向が確認された
(表2:非加熱・糖添加)。
(A) When sugar was added without heat treatment (non-heated, sugar added) To the solution obtained by ultrasonic treatment was added trehalose in an amount of about 1% to freeze-dry or spray. The dry powder obtained by drying or hot air drying showed a redissolution rate of 60 to 70% (Table 1: unheated, sugar added). And when preparing this dried product, the longer the period of cryopreservation,
It was confirmed that the redissolution rate of the obtained dried product tended to be poor (Table 2: non-heating and sugar addition).

【0049】(b)加熱処理を施し、糖を添加した場合
(加熱、糖添加) 一方、超音波処理によって得られた溶液を70℃、10分間
加熱処理し、更にトレハロースを約1%の濃度となるよ
うに添加し、凍結乾燥もしくは噴霧乾燥もしくは熱風乾
燥を行うことにより得られた乾燥物は、蒸留水に溶解さ
せると、ほぼ透明な溶液が得られ、70〜90%の再溶解率
を示した(表1:加熱・糖添加)。さらに、加熱処理を
し、糖を添加した場合では、凍結保存期間にかかわらず
ほぼ80%以上の再溶解率を示した。(表2:加熱・糖添
加)。
(B) When heat-treated and sugar added (heated, sugar added) On the other hand, the solution obtained by ultrasonic treatment was heat-treated at 70 ° C. for 10 minutes, and trehalose was added at a concentration of about 1%. The dried product obtained by freeze-drying or spray-drying or hot-air drying is dissolved in distilled water to obtain an almost transparent solution, and a redissolution rate of 70 to 90% is obtained. The results are shown in Table 1 (heating and addition of sugar). Furthermore, when heat treatment was performed and sugar was added, the re-dissolution rate was almost 80% or more regardless of the frozen storage period. (Table 2: Heating and sugar addition).

【0050】加熱処理をせず糖も添加しない場合(非加
熱、糖添加無し)、加熱処理をしたが糖は添加しない場
合(加熱、糖添加無し)は何れも、再溶解率は10〜30%
と低いことが確認された。
The redissolution rate was 10 to 30 in both cases where heat treatment was not performed and sugar was not added (non-heated, no sugar added), and when heat treatment was performed but sugar was not added (heated and no sugar added). %
It was confirmed to be low.

【0051】[0051]

【表1】 [Table 1]

【0052】[0052]

【表2】 [Table 2]

【0053】B.加熱条件下における糖の種類による再
溶解性への影響 表3は、加熱条件下における糖の種類による再溶解性へ
の影響を示す。超音波処理後、70℃、10分間加熱処理を
した溶液に対してトレハロース、ラフィノース、グルコ
ース、マルトース、フルクトース、シュクロース、デキ
ストリンを、それぞれ1%濃度になる量を添加し、例え
ば凍結乾燥を行うことにより乾燥粉末物を得た。この粉
末を水に溶解させるとほぼ透明な溶液が得られ、再溶解
率がほぼ70〜90%であることが確認された。同様の乾燥
粉末は、噴霧乾燥もしくは熱風乾燥でも得られた。添加
する糖は、単糖から多糖類まで、様々な種類の糖が有効
であった。
B. Effect of sugar type on resolubility under heating conditions Table 3 shows the effect of sugar type on resolubility under heating conditions. After sonication, trehalose, raffinose, glucose, maltose, fructose, sucrose, dextrin are added to the solution that has been heat-treated at 70 ° C for 10 minutes in an amount of 1% concentration, for example, freeze-dried. As a result, a dry powder product was obtained. When this powder was dissolved in water, an almost transparent solution was obtained, and it was confirmed that the redissolution rate was about 70 to 90%. Similar dry powders were obtained by spray drying or hot air drying. Various types of sugars were effective as added sugars, from monosaccharides to polysaccharides.

【0054】[0054]

【表3】 [Table 3]

【0055】C.加熱条件下における糖の濃度による再
溶解性への影響 次の表4は、加熱条件下における糖の濃度による再溶解
性への影響を実験した結果を示す。糖濃度0.5%では再
溶解率60%、糖濃度1%以上では再溶解率80%以上であ
った。再溶解可能な乾燥粉末を調製するためには、少な
くとも、0.5%以上の糖の添加が必要であった。
C. Effect of sugar concentration on resolubility under heating conditions Table 4 below shows the results of experiments on the effect of sugar concentration on resolubility under heating conditions. When the sugar concentration was 0.5%, the redissolution rate was 60%, and when the sugar concentration was 1% or higher, the redissolution rate was 80% or higher. In order to prepare a redissolvable dry powder, it was necessary to add at least 0.5% or more of sugar.

【0056】しかし、糖の濃度を増加させると、乾燥粉
末におけるタンパク質含量が減少し、利用法が限定され
てしまう可能性がある。従って、糖の添加量は1%前後
が望ましいと考えられる。
However, when the sugar concentration is increased, the protein content in the dry powder may be decreased, and the usage may be limited. Therefore, it is considered desirable to add sugar at around 1%.

【0057】[0057]

【表4】 [Table 4]

【0058】D.糖添加時の加熱温度が再溶解性に及ぼ
す影響 表5は、糖添加時の加熱温度が再溶解性に及ぼす影響を
実験した結果を示す。加熱処理温度が50℃、60℃の時
は、再溶解率は60%程度であった。加熱処理温度が70℃
以上の場合には、再溶解率は80%以上であった。したが
って、再溶解率をより向上させるためには糖の添加と70
℃以上の加熱処理が必要であった(表5)。
D. Effect of heating temperature during addition of sugar on resolubility Table 5 shows the results of experiments on the effect of heating temperature during addition of sugar on resolubility. When the heat treatment temperatures were 50 ° C and 60 ° C, the redissolution rate was about 60%. Heat treatment temperature is 70 ℃
In the above cases, the redissolution rate was 80% or more. Therefore, in order to further improve the redissolution rate, addition of sugar and 70%
A heat treatment at a temperature of ℃ or higher was required (Table 5).

【0059】[0059]

【表5】 [Table 5]

【0060】E.糖添加と加熱処理の順序の違いによる
再溶解性への影響 表6は、糖添加と加熱処理の順序の違いによる再溶解性
への影響を実験した結果を示す。タンパク質と糖を加熱
するとメイラード反応を起こすと言われている。そこ
で、糖を添加した後、加熱処理をすることにより、メイ
ラード反応の有無と再溶解率への影響を検討したが、供
試条件では、褐変は認められず、糖の添加と加熱処理の
順序は再溶解率に影響しないことがわかった。
E. Effect on Resolubility Due to Difference in Order of Sugar Addition and Heat Treatment Table 6 shows results of experiments on effect on resolubility due to difference in order of sugar addition and heat treatment. It is said that heating protein and sugar causes the Maillard reaction. Therefore, we examined the presence or absence of the Maillard reaction and the effect on the redissolution rate by adding sugar and then performing heat treatment.However, under the test conditions, browning was not observed, and the order of sugar addition and heat treatment was examined. Was found not to affect the redissolution rate.

【0061】[0061]

【表6】 [Table 6]

【0062】F.強い超音波処理による再溶解性への影
響 表7は、アクチン含量に対するミオシン含量の比と再溶
解性について調べた結果を示す。すなわち、既報の方法
に従って調製した水に溶解する食肉の溶液(試料番号
1。但し、超音波処理時間;5分間)、該溶液に超音波
処理時間を更に10、20と30分間延長して水に溶解
する食肉の溶液を調製した(それぞれ、試料番号2、3
と4)。そしてこれらの試料についてSDS-PAGEを常法に
従って行った。
F. Effect of strong sonication on resolubility Table 7 shows the results of investigating the ratio of myosin content to actin content and resolubility. That is, a solution of meat dissolved in water prepared according to the method previously reported (Sample No. 1, but ultrasonic treatment time: 5 minutes), and the ultrasonic treatment time was further extended to this solution for 10, 20 and 30 minutes. A solution of meat to be dissolved in each was prepared (Sample Nos. 2 and 3 respectively)
And 4). Then, SDS-PAGE was performed on these samples according to a conventional method.

【0063】SDS-PAGE像から、各試料のアクチン含量に
は殆ど変化のないことが認められた。一方、ミオシン含
量には顕著な差があることが認められた。そこで、SDS-
PAGEゲルについてデンシトメトリーを行い、アクチン含
量に対するミオシン含量の比を求めた。その結果を表7
に示す。又、各試料について乾燥粉末を調製し、その後
に乾燥粉末の再溶解率を測定した。その結果を表7に示
す。
From the SDS-PAGE image, it was confirmed that the actin content of each sample was almost unchanged. On the other hand, it was confirmed that there was a significant difference in myosin content. So SDS-
Densitometry was performed on the PAGE gel to determine the ratio of myosin content to actin content. The results are shown in Table 7.
Shown in. In addition, a dry powder was prepared for each sample, and thereafter the redissolution rate of the dry powder was measured. The results are shown in Table 7.

【0064】これらの測定結果から、アクチン含量に対
するミオシン含量の比が、例えば、1.89の場合には(試
料番号1)再溶解率は30.0%であり、当該比がより増加
すると再溶解率はより低下した。逆に、当該比が1.89よ
り低下するに従って再溶解率は向上し、例えば当該比が
0.85の時には(試料番号4)、再溶解率は86.1%であ
り、当該比が0.85以下になると再溶解率は更に高くなっ
た。
From these measurement results, when the ratio of the myosin content to the actin content is, for example, 1.89 (Sample No. 1), the redissolution rate is 30.0%, and the redissolution rate increases as the ratio increases. Fell. Conversely, the redissolution rate increases as the ratio decreases from 1.89, and
When it was 0.85 (Sample No. 4), the redissolution rate was 86.1%, and when the ratio was 0.85 or less, the redissolution rate was further increased.

【0065】[0065]

【表7】 [Table 7]

【0066】G.非中性化処理による再溶解性への影響 表8は、非中性化処理と再溶解性について調べた結果を
示す。すなわち、既報の方法に従って調製した水に溶解
する食肉の溶液(但し、超音波処理は無し)、該溶液の
pHを酸性からアルカリ性に、変えて水に溶解する食肉
の溶液を調製した。そしてこれらの試料について溶解率
の測定を行った。
G. Effect of Non-Neutralization Treatment on Resolubilization Table 8 shows the results of investigation on non-neutralization treatment and re-solubility. That is, a solution of meat dissolved in water prepared according to the previously reported method (however, no ultrasonic treatment), a solution of meat dissolved in water was prepared by changing the pH of the solution from acidic to alkaline. Then, the dissolution rate of these samples was measured.

【0067】[0067]

【表8】 [Table 8]

【0068】H.非中性化処理に用いる酸の種類による
再溶解性への影響 表9は、非中性化処理に用いる酸の種類と再溶解性につ
いて調べた結果を示す。すなわち、既報の方法に従って
調製した水に溶解する食肉の溶液(但し、超音波処理は
無し)、該溶液のpHを塩酸またはクエン酸を用いて変
えて水に溶解する食肉の溶液を調製した。そしてこれら
の試料について溶解率の測定を行った。
H. Effect of type of acid used for non-neutralization treatment on resolubility Table 9 shows the results of examination on the type of acid used for non-neutralization treatment and resolubility. That is, a solution of meat dissolved in water prepared according to the previously reported method (however, no ultrasonic treatment), a solution of meat dissolved in water was prepared by changing the pH of the solution using hydrochloric acid or citric acid. Then, the dissolution rate of these samples was measured.

【0069】[0069]

【表9】 [Table 9]

【0070】以上、本発明に係る水可溶性食肉調製品の
形態を実施例に基づいて説明したが、本発明は特にこの
ような実施例に限定されることなく、特許請求の範囲記
載の技術的事項の範囲内でいろいろな実施例があること
はいうまでもない。
Although the form of the water-soluble meat preparation according to the present invention has been described above based on the embodiments, the present invention is not particularly limited to such embodiments, and the technical scope described in the claims is not limited thereto. It goes without saying that there are various examples within the scope of matters.

【0071】[0071]

【発明の効果】以上の構成から成る本発明に係る水可溶
性食肉調製品は、水に容易に溶解し、様々な食品への利
用が可能となり、食品産業に多大の寄与する。そして、
本発明に係る水可溶性食肉調製品は、食肉タンパク質の
溶液を加熱処理後、凝集物を遠心分離により除去するこ
とにより得られた溶液に、糖の添加や加熱処理を行い、
その後保存性賦与処理を行うことにより得られる、保存
性を有すると共に容易に水可溶化することができる。
The water-soluble meat preparation according to the present invention having the above-mentioned constitution is easily dissolved in water and can be used for various foods, which greatly contributes to the food industry. And
The water-soluble meat preparation according to the present invention, after the heat treatment of the solution of the meat protein, the solution obtained by removing the aggregate by centrifugation, the addition of sugar and heat treatment,
After that, it has a preservative property and can be easily solubilized in water, which is obtained by performing a preservative imparting treatment.

【0072】又、本発明に係る水可溶性食肉調製品は、
食肉タンパク質の溶液を加熱処理後、凝集物を遠心分離
により除去することにより得られた溶液に、より強い超
音波を加え、その後、保存性賦与処理を行うことにより
得られる、保存性を有すると共に容易に水可溶化するこ
とができる。
Further, the water-soluble meat preparation according to the present invention is
After the heat treatment of the solution of meat protein, the solution obtained by removing the aggregates by centrifugation is subjected to stronger ultrasonic waves, and then obtained by performing the preservability imparting property, which has a preserving property. It can be easily solubilized in water.

【0073】更に、本発明に係る水可溶性食肉調製品
は、食肉タンパク質の溶液を加熱処理後、凝集物を遠心
分離により除去することにより得られた溶液に、酸性も
しくはアルカリ性へのpH調整を行い、その後、保存性
賦与処理を行うことにより得られる、保存性を有すると
共に容易に水可溶化することができる。
Furthermore, the water-soluble meat preparation according to the present invention is prepared by subjecting a solution of meat protein to a heat treatment and then removing aggregates by centrifugation to adjust the pH of the solution to acidic or alkaline. After that, it has a preservability and can be easily solubilized in water, which is obtained by carrying out a preservability imparting treatment.

───────────────────────────────────────────────────── フロントページの続き (72)発明者 伊藤 友季子 茨城県つくば市緑ヶ原3丁目3番地 日本 ハム株式会社中央研究所内 (72)発明者 井手 弘 茨城県つくば市緑ヶ原3丁目3番地 日本 ハム株式会社中央研究所内 (72)発明者 服部 昭仁 北海道札幌市南区石山3条6丁目1−13 Fターム(参考) 4B042 AD39 AH01 AK07 AP02 AP17 AP18 AP20 AP30    ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Yukiko Ito             3-3 Midorigahara, Tsukuba, Ibaraki Japan             Central Research Laboratory, Ham Co., Ltd. (72) Inventor Hiroshi Ide             3-3 Midorigahara, Tsukuba, Ibaraki Japan             Central Research Laboratory, Ham Co., Ltd. (72) Inventor Akito Hattori             3-6-6, Ishiyama, Minami-ku, Sapporo-shi, Hokkaido F term (reference) 4B042 AD39 AH01 AK07 AP02 AP17                       AP18 AP20 AP30

Claims (8)

【特許請求の範囲】[Claims] 【請求項1】 水に溶解させた食肉に保存性賦与処理
を行って得られる水可溶性食肉調製品であって、 上記保存性賦与処理に先立ち糖が添加されていることに
より水に対し高い再溶解性を有することを特徴とする水
可溶性食肉調製品。
1. A water-soluble meat preparation obtained by subjecting meat dissolved in water to a preservative-imparting treatment, wherein sugar is added prior to the preserving-imparting treatment, so that it is highly reproducible to water. A water-soluble meat preparation characterized by having solubility.
【請求項2】 水に溶解させた食肉に保存性賦与処理
を行って得られる水可溶性食肉調製品であって、 上記保存性賦与処理に先立ち、加熱され、且つ糖が添加
されていることにより水に対し高い再溶解性を有するこ
とを特徴とする水可溶性食肉調製品。
2. A water-soluble meat preparation obtained by subjecting meat dissolved in water to a preservative imparting treatment, which comprises heating and adding sugar prior to the preservative imparting treatment. A water-soluble meat preparation characterized by having high resolubility in water.
【請求項3】 水に溶解させた食肉に保存性賦与処理
を行って得られる水可溶性食肉調製品であって、 上記保存性賦与処理に先立ち、超音波処理されているこ
とにより水に対し高い再溶解性を有することを特徴とす
る水可溶性食肉調製品。
3. A water-soluble meat preparation obtained by subjecting meat dissolved in water to a preservative-imparting treatment, which is high in water due to ultrasonic treatment prior to the preserving-imparting treatment. A water-soluble meat preparation characterized by having resolubility.
【請求項4】 上記保存性賦与処理に先立つ上記超音
波処理は、上記食肉の筋束、筋繊維もしくは筋原線維か
ら成る構造体、又は該構造体を構成するタンパク質群の
分子構造を、弛緩した状態もしくは低分子化した状態に
することのできる仕事量の超音波処理であることを特徴
とする請求項3記載の水可溶性食肉調製品。
4. The ultrasonic treatment prior to the preservation-imparting treatment relaxes the molecular structure of muscle bundles of muscle, muscle fibers or myofibrils, or the molecular structure of proteins constituting the structure. 4. The water-soluble meat preparation according to claim 3, wherein the water-soluble meat preparation is sonicated with a work amount capable of being brought into a conditioned state or a low molecular weight state.
【請求項5】 上記保存性賦与処理に先立つ上記超音
波処理は、上記食肉の筋原線維を構成するタンパク質で
あるミオシンの低分子化を生じさせることのできる仕事
量の超音波処理であることを特徴とする請求項3記載の
水可溶性食肉調製品。
5. The ultrasonic treatment prior to the preservation-imparting treatment is an ultrasonic treatment with a work amount capable of causing a reduction in molecular weight of myosin, which is a protein constituting the myofibril of the meat. The water-soluble meat preparation according to claim 3.
【請求項6】 水に溶解させた食肉に保存性賦与処理
を行って得られる水可溶性食肉調製品であって、 上記保存性賦与処理に先立ち、非中性化処理されている
ことにより水に対し高い再溶解性を有することを特徴と
する水可溶性食肉調製品。
6. A water-soluble meat preparation obtained by subjecting meat dissolved in water to a preservative-imparting treatment, wherein the preservative-imparting treatment prior to the preservative-imparting treatment results in a non-neutralizing treatment. A water-soluble meat preparation characterized by having high re-solubility.
【請求項7】 上記非中性化処理が、pH5以下にす
ること、又はpH7.5以上にする処理であることを特
徴とする請求項6に記載の水可溶性食肉調製品。
7. The water-soluble meat preparation according to claim 6, wherein the non-neutralizing treatment is a treatment of adjusting the pH to 5 or lower, or to adjusting the pH to 7.5 or higher.
【請求項8】 上記保存性賦与処理は、冷蔵、冷凍、
加熱殺菌、高温加熱殺菌、超高圧殺菌、超音波殺菌又は
乾燥処理であることを特徴とする請求項1〜7のいずれ
かに記載の水溶性食肉調製品。
8. The preservative imparting treatment is refrigerated, frozen,
Heat-sterilization, high-temperature heat sterilization, ultra-high pressure sterilization, ultrasonic sterilization, or a drying treatment, The water-soluble meat preparation according to claim 1.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007513640A (en) * 2003-12-16 2007-05-31 プロテウス・インダストリーズ・インコーポレーテッド Food product and method for reducing fat and fat content in cooked ingredients
KR100965479B1 (en) * 2007-10-29 2010-06-24 한국식품연구원 Method of making myoprotein water-soluble by Using Sonication
KR101242536B1 (en) * 2011-01-31 2013-03-18 한국식품연구원 A methohd of solubilizing myofibrillar protein and a method of producing protein for food
KR101284217B1 (en) * 2011-03-15 2013-07-09 한국식품연구원 A methohd of solubilizing muscle protein and a method of producing protein for food
KR101322307B1 (en) 2011-10-31 2013-10-29 대한민국 Diet compositions containing myofibrill and manufacturing method thereof
CN112552370A (en) * 2020-12-11 2021-03-26 中国药科大学 Method for extracting water-soluble protein from shrimp meat

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007513640A (en) * 2003-12-16 2007-05-31 プロテウス・インダストリーズ・インコーポレーテッド Food product and method for reducing fat and fat content in cooked ingredients
JP4705588B2 (en) * 2003-12-16 2011-06-22 プロテウス・インダストリーズ・インコーポレーテッド Food product and method for reducing fat and fat content in cooked ingredients
KR100965479B1 (en) * 2007-10-29 2010-06-24 한국식품연구원 Method of making myoprotein water-soluble by Using Sonication
KR101242536B1 (en) * 2011-01-31 2013-03-18 한국식품연구원 A methohd of solubilizing myofibrillar protein and a method of producing protein for food
KR101284217B1 (en) * 2011-03-15 2013-07-09 한국식품연구원 A methohd of solubilizing muscle protein and a method of producing protein for food
KR101322307B1 (en) 2011-10-31 2013-10-29 대한민국 Diet compositions containing myofibrill and manufacturing method thereof
CN112552370A (en) * 2020-12-11 2021-03-26 中国药科大学 Method for extracting water-soluble protein from shrimp meat

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