ES2225992T3 - Nuevos complejos polimericos para la transfeccion de acidos nucleicos, con residuos que desestabilizan unas membranas celulares. - Google Patents
Nuevos complejos polimericos para la transfeccion de acidos nucleicos, con residuos que desestabilizan unas membranas celulares.Info
- Publication number
- ES2225992T3 ES2225992T3 ES97945903T ES97945903T ES2225992T3 ES 2225992 T3 ES2225992 T3 ES 2225992T3 ES 97945903 T ES97945903 T ES 97945903T ES 97945903 T ES97945903 T ES 97945903T ES 2225992 T3 ES2225992 T3 ES 2225992T3
- Authority
- ES
- Spain
- Prior art keywords
- residues
- cells
- polymer
- free
- polylysine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- AHLPHDHHMVZTML-BYPYZUCNSA-N ornithyl group Chemical group N[C@@H](CCCN)C(=O)O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- 201000008968 osteosarcoma Diseases 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 108010064470 polyaspartate Proteins 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229960005179 primaquine Drugs 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000005460 tetrahydrofolate Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- WHNFPRLDDSXQCL-UAZQEYIDSA-N α-msh Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)NC(=O)[C@H](CO)NC(C)=O)C1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UAZQEYIDSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9613990 | 1996-11-15 | ||
| FR9613990A FR2755976B1 (fr) | 1996-11-15 | 1996-11-15 | Nouveaux complexes d'acides nucleiques et de polymere substitue par des residus entrainant la destabilisation des membranes cellulaires |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2225992T3 true ES2225992T3 (es) | 2005-03-16 |
Family
ID=9497684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97945903T Expired - Lifetime ES2225992T3 (es) | 1996-11-15 | 1997-11-10 | Nuevos complejos polimericos para la transfeccion de acidos nucleicos, con residuos que desestabilizan unas membranas celulares. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6372499B1 (https=) |
| EP (1) | EP0946744B1 (https=) |
| JP (1) | JP2001504344A (https=) |
| AT (1) | ATE274066T1 (https=) |
| AU (1) | AU742818B2 (https=) |
| CA (1) | CA2267833A1 (https=) |
| DE (1) | DE69730348T2 (https=) |
| ES (1) | ES2225992T3 (https=) |
| FR (1) | FR2755976B1 (https=) |
| IL (1) | IL128912A (https=) |
| WO (1) | WO1998022610A1 (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6919076B1 (en) | 1998-01-20 | 2005-07-19 | Pericor Science, Inc. | Conjugates of agents and transglutaminase substrate linking molecules |
| AU770963B2 (en) * | 1998-12-02 | 2004-03-11 | I.D.M. Immuno-Designed Molecules | New oligomeric conjugates liable to transfer biological molecules into cells |
| AU7352200A (en) * | 1999-09-01 | 2001-03-26 | Regents Of The University Of California, The | Novel natriuretic peptide receptors, interacting compounds and methods of regulating proliferation and/or survival of neurons |
| US7070807B2 (en) | 1999-12-29 | 2006-07-04 | Mixson A James | Branched histidine copolymers and methods for using same |
| WO2001047496A1 (en) * | 1999-12-29 | 2001-07-05 | Mixson A James | Histidine copolymer and methods for using same |
| JP5610659B2 (ja) * | 2000-07-21 | 2014-10-22 | ルバンス セラピュティックス インク．Ｒｅｖａｎｃｅ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 多成分生物学的輸送システム |
| FR2812658B1 (fr) * | 2000-08-01 | 2005-04-15 | De Bruno Vandiere | Procede d'analyse d'acides nucleiques |
| US7465708B2 (en) | 2002-11-25 | 2008-12-16 | Mixson A James | Branched cationic copolymers and methods for antimicrobial use |
| US9211248B2 (en) | 2004-03-03 | 2015-12-15 | Revance Therapeutics, Inc. | Compositions and methods for topical application and transdermal delivery of botulinum toxins |
| JP2007527431A (ja) | 2004-03-03 | 2007-09-27 | ルバンス セラピュティックス | 局所的診断及び治療用の輸送のための組成物及び方法 |
| US7740861B2 (en) * | 2004-06-16 | 2010-06-22 | University Of Massachusetts | Drug delivery product and methods |
| US20060040879A1 (en) * | 2004-08-21 | 2006-02-23 | Kosak Kenneth M | Chloroquine coupled nucleic acids and methods for their synthesis |
| AU2005284727A1 (en) * | 2004-09-17 | 2006-03-23 | University Of Massachusetts | Compositions and their uses for lysosomal enzyme deficiencies |
| US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| US20060211004A1 (en) | 2005-02-15 | 2006-09-21 | Ilsley Diane D | Methods and compositions for determining non-specific cytotoxicity of a transfection agent |
| ZA200707352B (en) | 2005-03-03 | 2009-04-29 | Revance Therapeutics Inc | Compositions and methods for topical application and transdermal delivery of botulinum toxins |
| ATE526991T1 (de) * | 2005-10-24 | 2011-10-15 | Univ Massachusetts | Zusammensetzungen und ihre verwendungen für die gentherapeutische behandlung von knochenerkrankungen |
| EP2222283A2 (en) * | 2007-10-29 | 2010-09-01 | University of Massachusetts | Yeast cell wall protein (ycwp) encapsulated multilayered nanoparticles for nucleic acid delivery (sirna) |
| FR2928373B1 (fr) * | 2008-03-05 | 2010-12-31 | Centre Nat Rech Scient | Polymere derive de la polyethylenimine lineaire pour le transfert de gene. |
| US8815818B2 (en) | 2008-07-18 | 2014-08-26 | Rxi Pharmaceuticals Corporation | Phagocytic cell delivery of RNAI |
| EP2342340A1 (en) | 2008-09-22 | 2011-07-13 | Rxi Pharmaceuticals Corporation | Rna interference in skin indications |
| WO2010078536A1 (en) | 2009-01-05 | 2010-07-08 | Rxi Pharmaceuticals Corporation | Inhibition of pcsk9 through rnai |
| US20120277155A1 (en) | 2011-02-25 | 2012-11-01 | Medtronic, Inc. | Therapy for kidney disease and/or heart failure |
| EP2750697A4 (en) | 2011-09-02 | 2015-03-25 | Medtronic Inc | CHIMERIC NATRIURETIC PEPTIDE COMPOSITIONS AND METHOD FOR THE PRODUCTION THEREOF |
| US9162191B2 (en) * | 2012-11-15 | 2015-10-20 | Board Of Trustees Of The University Of Alabama | Imidazole-containing polymer membranes and methods of use |
| CN102949729B (zh) * | 2012-11-20 | 2014-06-18 | 中国科学院宁波材料技术与工程研究所 | 主动靶向型小干扰核糖核酸输送载体及其制备方法 |
| US12544344B2 (en) | 2017-04-19 | 2026-02-10 | Phio Pharmaceuticals Corp. | Topical delivery of nucleic acid compounds |
| AR122446A1 (es) * | 2020-04-29 | 2022-09-14 | Medimmune Llc | Composiciones y métodos para suministrar agentes farmacéuticamente activos |
| US20240050582A1 (en) * | 2021-03-08 | 2024-02-15 | Universiteit Gent | Conjugates including multiple saccharidic chains on a linear protein and uses in mammals feed |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1316990A (en) * | 1970-09-17 | 1973-05-16 | Roche Products Ltd | Imidazolyl derivatives |
| US5166320A (en) * | 1987-04-22 | 1992-11-24 | University Of Connecticut | Carrier system and method for the introduction of genes into mammalian cells |
| JP2827287B2 (ja) * | 1988-07-05 | 1998-11-25 | 武田薬品工業株式会社 | 水溶性薬物含有徐放型マイクロカプセル |
| US5354844A (en) * | 1989-03-16 | 1994-10-11 | Boehringer Ingelheim International Gmbh | Protein-polycation conjugates |
| DE59010432D1 (de) * | 1989-03-16 | 1996-09-05 | Boehringer Ingelheim Int | Genetische Einheiten zur Inhibierung der Funktion von RNA |
| EP0563249B1 (en) * | 1990-12-19 | 1997-04-23 | Advanced Magnetics Incorporated | Targeting of therapeutic agents using polysaccharides |
| DE4104186A1 (de) * | 1991-02-12 | 1992-08-13 | Genentech Inc | Neue, ueber endozytose in hoehere eukaryotische zellen aufnehmbare, nukleinsaeure enthaltende komplexe |
| EP0610441A4 (en) * | 1991-10-29 | 1996-01-10 | Clover Cons Ltd | CROSSLINKABLE POLYSACCHARIDES, POLYCATIONS AND LIPIDS CAN BE USED TO ENCODE AND DISPENSE MEDICINAL PRODUCTS. |
| WO1993019768A1 (en) * | 1992-04-03 | 1993-10-14 | The Regents Of The University Of California | Self-assembling polynucleotide delivery system |
| US5733762A (en) * | 1994-04-28 | 1998-03-31 | I.D.M. Immuno-Designed Molecules | Complexes of nucleic acid and polymer, their process of preparation and their use for the transfection of cells |
| FR2719316B1 (fr) * | 1994-04-28 | 1996-05-31 | Idm | Nouveaux complexes d'acide nucléique et de polymère, leur procédé de préparation et leur utilisation pour la transfection de cellules. |
| US6030941A (en) * | 1996-05-01 | 2000-02-29 | Avi Biopharma, Inc. | Polymer composition for delivering substances in living organisms |
-
1996
- 1996-11-15 FR FR9613990A patent/FR2755976B1/fr not_active Expired - Fee Related
-
1997
- 1997-11-10 EP EP97945903A patent/EP0946744B1/fr not_active Expired - Lifetime
- 1997-11-10 ES ES97945903T patent/ES2225992T3/es not_active Expired - Lifetime
- 1997-11-10 IL IL128912A patent/IL128912A/en not_active IP Right Cessation
- 1997-11-10 JP JP52325798A patent/JP2001504344A/ja not_active Ceased
- 1997-11-10 WO PCT/FR1997/002022 patent/WO1998022610A1/fr not_active Ceased
- 1997-11-10 AT AT97945903T patent/ATE274066T1/de not_active IP Right Cessation
- 1997-11-10 AU AU51239/98A patent/AU742818B2/en not_active Ceased
- 1997-11-10 DE DE69730348T patent/DE69730348T2/de not_active Expired - Fee Related
- 1997-11-10 CA CA002267833A patent/CA2267833A1/fr not_active Abandoned
- 1997-11-10 US US09/297,519 patent/US6372499B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998022610A1 (fr) | 1998-05-28 |
| EP0946744A1 (fr) | 1999-10-06 |
| IL128912A0 (en) | 2000-02-17 |
| DE69730348D1 (de) | 2004-09-23 |
| IL128912A (en) | 2006-06-11 |
| CA2267833A1 (fr) | 1998-05-28 |
| EP0946744B1 (fr) | 2004-08-18 |
| US6372499B1 (en) | 2002-04-16 |
| AU742818B2 (en) | 2002-01-10 |
| ATE274066T1 (de) | 2004-09-15 |
| DE69730348T2 (de) | 2005-09-01 |
| AU5123998A (en) | 1998-06-10 |
| FR2755976A1 (fr) | 1998-05-22 |
| JP2001504344A (ja) | 2001-04-03 |
| FR2755976B1 (fr) | 1999-01-15 |
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