ES2200696B1 - Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo. - Google Patents

Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo.

Info

Publication number
ES2200696B1
ES2200696B1 ES200201437A ES200201437A ES2200696B1 ES 2200696 B1 ES2200696 B1 ES 2200696B1 ES 200201437 A ES200201437 A ES 200201437A ES 200201437 A ES200201437 A ES 200201437A ES 2200696 B1 ES2200696 B1 ES 2200696B1
Authority
ES
Spain
Prior art keywords
cysdv
plants
resistance against
nucleotide sequence
virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200201437A
Other languages
English (en)
Other versions
ES2200696A1 (es
Inventor
Maribel Franco Redrejo
Juan Manuel Aguilar Aguilar
Cristina Frenandez Marco
Juan Diaz Pendon
Emilio Rodriguez Cerezo
Miguel Angel Aranda Regules
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200201437A priority Critical patent/ES2200696B1/es
Priority to AU2003242791A priority patent/AU2003242791A1/en
Priority to PCT/ES2003/000307 priority patent/WO2004001046A1/es
Publication of ES2200696A1 publication Critical patent/ES2200696A1/es
Application granted granted Critical
Publication of ES2200696B1 publication Critical patent/ES2200696B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Método para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitáceas (CYSDV) en plantas, construcciones genéticas usadas y plantas resistentes a CYSDV obtenidas mediante dicho método. Se describe una construcción de ácido nucleico que comprende una primera secuencia de nucleótidos capaz de regular la transcripción en una planta de una segunda secuencia de nucleótidos, comprendiendo dicha segunda secuencia de nucleótidos: (i) una secuencia de nucleótidos correspondiente a un fragmento del RNA1 del Virus del amarilleo y enanismo de las cucurbitáceas (CYSDV), y (ii) una repetición invertida de la totalidad o parte de dicha secuencia de nucleótidos (i). Dicha construcción puede utilizarse para generar resistencia frente a CYSDV en plantas susceptibles a la infección por dicho virus.
ES200201437A 2002-06-21 2002-06-21 Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo. Expired - Fee Related ES2200696B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES200201437A ES2200696B1 (es) 2002-06-21 2002-06-21 Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo.
AU2003242791A AU2003242791A1 (en) 2002-06-21 2003-06-20 Method of generating resistance against cucurbit yellow stunting disorder virus (cysdv) in plants, genetic constructions used and cysdv-resistant plants thus produced
PCT/ES2003/000307 WO2004001046A1 (es) 2002-06-21 2003-06-20 Método para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitáceas (cysdv) en plantas, construcciones genéticas usadas y plantas resistentes a cysdv obtenidas mediante dicho método

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200201437A ES2200696B1 (es) 2002-06-21 2002-06-21 Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo.

Publications (2)

Publication Number Publication Date
ES2200696A1 ES2200696A1 (es) 2004-03-01
ES2200696B1 true ES2200696B1 (es) 2005-06-16

Family

ID=29797396

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200201437A Expired - Fee Related ES2200696B1 (es) 2002-06-21 2002-06-21 Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo.

Country Status (3)

Country Link
AU (1) AU2003242791A1 (es)
ES (1) ES2200696B1 (es)
WO (1) WO2004001046A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9578824B2 (en) 2014-10-10 2017-02-28 Seminis Vegetable Seeds, Inc. Melon hybrid SV5845MP and parents thereof
EP3005862A1 (en) * 2014-10-10 2016-04-13 Seminis Vegetable Seeds, Inc. Melon plants with improved disease tolerance
US9609816B2 (en) 2014-10-10 2017-04-04 Seminis Vegetable Seeds, Inc. Melon hybrid SV5133MG and parents thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0804583A2 (en) * 1993-07-09 1997-11-05 Asgrow Seed Company Lettuce infectious yellows virus genes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CELIX, ANA et al. Characterizacion of cucurbit yellow stunting disorder virus, a Bemisia tabaci-transmitted closterovirus. Phytopathology, (1996), 86 (12), 1370-1376. (Citado en la solicitud) *
KLAASSEN V.A. et al. Genome structure and phylogenetic analysis of lettuce infectious yellowsvirus, a whitefly-transmitted, bipartite closterovirus. Virology, 1995, 208 (1): 99-110. *
WATERHOUSE et al. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl. Acad. Sci., 1998, Vol. 95, páginas 13959-13964. (Citado en la solicitud) *

Also Published As

Publication number Publication date
ES2200696A1 (es) 2004-03-01
AU2003242791A1 (en) 2004-01-06
WO2004001046A1 (es) 2003-12-31

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ES2200696B1 (es) Metodo para generar resistencia frente al virus del amarilleo y enanismo de las cucurbitaceas (cysdv) en plantas, construcciones geneticas usadas y plantas resistentes a cysdv obtenidas mediante dicho metodo.

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