ES2195728A1 - Metodo para la deteccion de antigenos de fasciola hepatica en heces basado en un test elisa-sandwich - Google Patents
Metodo para la deteccion de antigenos de fasciola hepatica en heces basado en un test elisa-sandwichInfo
- Publication number
- ES2195728A1 ES2195728A1 ES200101575A ES200101575A ES2195728A1 ES 2195728 A1 ES2195728 A1 ES 2195728A1 ES 200101575 A ES200101575 A ES 200101575A ES 200101575 A ES200101575 A ES 200101575A ES 2195728 A1 ES2195728 A1 ES 2195728A1
- Authority
- ES
- Spain
- Prior art keywords
- antibodies
- detection
- contacting
- sandwich test
- antigens via
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 title abstract 3
- 102000036639 antigens Human genes 0.000 title abstract 3
- 108091007433 antigens Proteins 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 210000003608 fece Anatomy 0.000 title abstract 2
- 241000242711 Fasciola hepatica Species 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Método para la detección de antígenos de Fasciola hepática en heces basado en un test elisa-sandwich. El método comprende (a) poner en contacto una muestra procedente de las heces del animal a ensayar con anticuerpos fijados a un soporte sólido que reconocen antígenos de F. hepática bajo condiciones que permiten la formación de un complejo antígeno-anticuerpo, (b) añadir anticuerpos marcados que reconocen antígenos de F. hepática, y (c) revelar la reacción mediante la adición de un sustrato específico para el marcador. De aplicación en el diagnóstico de F. hepática.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101575A ES2195728B1 (es) | 2001-07-05 | 2001-07-05 | Metodo para la deteccion de antigenos de fasciola hepatica en heces basado en un test elisa-sandwich. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101575A ES2195728B1 (es) | 2001-07-05 | 2001-07-05 | Metodo para la deteccion de antigenos de fasciola hepatica en heces basado en un test elisa-sandwich. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2195728A1 true ES2195728A1 (es) | 2003-12-01 |
ES2195728B1 ES2195728B1 (es) | 2004-10-16 |
Family
ID=29762944
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200101575A Expired - Fee Related ES2195728B1 (es) | 2001-07-05 | 2001-07-05 | Metodo para la deteccion de antigenos de fasciola hepatica en heces basado en un test elisa-sandwich. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2195728B1 (es) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5338660A (en) * | 1991-07-12 | 1994-08-16 | Board Of Supervisors Of Louisiana State University And Agricutural And Mechanical College | Diagnosis of Fasciola infections by detection of antigens in feces or intestinal content |
WO2000017654A1 (en) * | 1998-09-21 | 2000-03-30 | Cummings Richard D | Assays for helminth infections |
-
2001
- 2001-07-05 ES ES200101575A patent/ES2195728B1/es not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5338660A (en) * | 1991-07-12 | 1994-08-16 | Board Of Supervisors Of Louisiana State University And Agricutural And Mechanical College | Diagnosis of Fasciola infections by detection of antigens in feces or intestinal content |
WO2000017654A1 (en) * | 1998-09-21 | 2000-03-30 | Cummings Richard D | Assays for helminth infections |
US6261788B1 (en) * | 1998-09-21 | 2001-07-17 | The Board Of Regents Of The University Of Oklahoma | Diagnostic assays for infectious parasitic helminths |
Non-Patent Citations (1)
Title |
---|
B.E. DUM+NIGO; M. MEZO. Monoclonal Antibody sandwich inmunoassay detection of copoantigen to evaluate the efficacy of treatment in natural ovine fasciolisis. Research in Veterinary Science, 1999, 66, pßginas 1965-1967. * |
Also Published As
Publication number | Publication date |
---|---|
ES2195728B1 (es) | 2004-10-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 2195728B1 Country of ref document: ES |
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FD2A | Announcement of lapse in spain |
Effective date: 20180807 |