ES2193215T3 - Procedimiento y reactivo para la determinacion especifica del mrna. - Google Patents

Procedimiento y reactivo para la determinacion especifica del mrna.

Info

Publication number
ES2193215T3
ES2193215T3 ES96111586T ES96111586T ES2193215T3 ES 2193215 T3 ES2193215 T3 ES 2193215T3 ES 96111586 T ES96111586 T ES 96111586T ES 96111586 T ES96111586 T ES 96111586T ES 2193215 T3 ES2193215 T3 ES 2193215T3
Authority
ES
Spain
Prior art keywords
opt
mixt
heat
buffer
vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96111586T
Other languages
English (en)
Inventor
Hermann Dr Leying
Matthias Dr Hinzpeter
Hans-Peter Dr Fritton
Heiko Wittor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of ES2193215T3 publication Critical patent/ES2193215T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION DE MARN, QUE SE CARACTERIZA ESENCIALMENTE PORQUE SE UNE UN HIBRIDO FORMADO EN UNA SOLUCION A UNA FASE SOLIDA QUE ES ESTABLE AL CALOR, Y A CONTINUACION SE LLEVA A CABO UNA REACCION DE TRANSCRIPTASA INVERSA Y UNA DE PCR EN EL MISMO RECIPIENTE. SE HA VISTO QUE ES ESPECIALMENTE VENTAJOSO, SI EL OLIGONUCLEOTIDO NECESARIO PARA LA REACCION SE MARCA CON BIOTINA, LA FASE SOLIDA SE RECUBRE CON ESTREPTAVIDINA O AVIDINA, Y LA REACCION SE LLEVA A CABO SIN RIBONUCLEASA. ADEMAS SE DESCRIBE EL CORRESPONDIENTE EQUIPO.
ES96111586T 1995-07-21 1996-07-18 Procedimiento y reactivo para la determinacion especifica del mrna. Expired - Lifetime ES2193215T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19526431A DE19526431A1 (de) 1995-07-21 1995-07-21 Verfahren und Reagenz zur spezifischen Bestimmung von mRNA

Publications (1)

Publication Number Publication Date
ES2193215T3 true ES2193215T3 (es) 2003-11-01

Family

ID=7767284

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96111586T Expired - Lifetime ES2193215T3 (es) 1995-07-21 1996-07-18 Procedimiento y reactivo para la determinacion especifica del mrna.

Country Status (7)

Country Link
US (1) US5955271A (es)
EP (1) EP0754764B1 (es)
JP (1) JPH0928398A (es)
AT (1) ATE236995T1 (es)
CA (1) CA2181417C (es)
DE (2) DE19526431A1 (es)
ES (1) ES2193215T3 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004755A (en) * 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
AT414209B (de) * 2000-03-17 2006-10-15 Greiner Bio One Gmbh Sammelgefäss für flüssigkeiten
US20030098271A1 (en) * 2001-11-26 2003-05-29 Ralph Somack Capsule and tray systems for combined sample collection, archiving, purification, and PCR
CN1316036C (zh) * 2003-01-06 2007-05-16 徐定邦 一种以聚合酶链式反应为基础的基因定量方法
EP1631668A1 (en) * 2003-06-06 2006-03-08 Applera Corporation Purification device for ribonucleic acid in large volumes, and method
US20110287951A1 (en) * 2009-01-30 2011-11-24 Emmert-Buck Michael R Methods and systems for purifying, transferring, and/or manipulating nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
IE64286B1 (en) * 1988-05-25 1995-07-26 Boehringer Mannheim Gmbh Process for the determination of an immunologically detectable substance and a suitable vessel therefor
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
JP3046837B2 (ja) * 1989-03-10 2000-05-29 バイシス・インコーポレーテツド 固定化されたオリゴヌクレオチドのプローブおよびそれらの使用
KR920700360A (ko) * 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
EP0672173B1 (en) * 1991-11-01 2002-08-28 Diatech Pty. Ltd. Solid phase amplification process
JPH08500722A (ja) * 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
US5424189A (en) * 1993-03-05 1995-06-13 Kansas State University Research Foundation Bovine respiratory syncytial virus detection and primers
JPH06261800A (ja) * 1993-03-10 1994-09-20 Hitachi Ltd Ｒｎａウィルスの検出方法

Also Published As

Publication number Publication date
JPH0928398A (ja) 1997-02-04
CA2181417C (en) 2009-09-22
DE19526431A1 (de) 1997-01-23
ATE236995T1 (de) 2003-04-15
CA2181417A1 (en) 1997-01-22
US5955271A (en) 1999-09-21
DE59610324D1 (de) 2003-05-15
EP0754764A3 (de) 1999-04-21
EP0754764B1 (de) 2003-04-09
EP0754764A2 (de) 1997-01-22

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