ES2185493A1 - Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. - Google Patents

Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.

Info

Publication number
ES2185493A1
ES2185493A1 ES200101545A ES200101545A ES2185493A1 ES 2185493 A1 ES2185493 A1 ES 2185493A1 ES 200101545 A ES200101545 A ES 200101545A ES 200101545 A ES200101545 A ES 200101545A ES 2185493 A1 ES2185493 A1 ES 2185493A1
Authority
ES
Spain
Prior art keywords
angiogenesis
relates
compounds
evaluating
developed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200101545A
Other languages
English (en)
Other versions
ES2185493B1 (es
Inventor
Vallina Luis Alvarez
Alcober Laura Sanz
Carrasco Mario Pascual
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Madrid Genetics S L
Original Assignee
Madrid Genetics S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Madrid Genetics S L filed Critical Madrid Genetics S L
Priority to ES200101545A priority Critical patent/ES2185493B1/es
Priority to PCT/ES2002/000327 priority patent/WO2003005301A1/es
Publication of ES2185493A1 publication Critical patent/ES2185493A1/es
Application granted granted Critical
Publication of ES2185493B1 publication Critical patent/ES2185493B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método para evaluar in vitro en condiciones fisiológicas o patológicas relevantes la actividad biológica de compuestos a gran escala. La formación de vasos sanguíneos (angiogénesis) es un proceso necesario para el crecimiento y diseminación (metástasis) de los tumores. El desarrollo de sistemas de examen "screening" para la identificación de compuestos inhibidores de este proceso es un campo de investigación exhaustiva, que en los últimos años camina hacia el uso de sistemas informáticos y robotizados. Se presenta un sistema de adquisición y procesamiento de imágenes, diseñado y desarrollado para ser el núcleo de una plataforma dirigida a escenarios de "screening" masivo en experimentos basados en modelos de angiogénesis "in vitro"'' miniaturizados. El sistema adquiere y procesa secuencialmente imágenes obtenidas mediante microscopio y cámara digital de los pocillos de microplacas estándar. Se propone un procedimiento de cuantificación del grado de angiogénesis basado en un análisis morfológico y topológico de la red de vasos generados, que proporciona un valor añadido al tradicional parámetro de densidad microvascular. En su cálculo se toman en consideración factores adicionales como conectividad y longitud de los vasos. El sistema cumple con los requerimientos básicos en estos experimentos: objetividad, reproducibilidad y rapidez; alcanzados por la capacidad automática de cuantificación de la actividad angiogénica desarrollada.
ES200101545A 2001-07-03 2001-07-03 Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala. Expired - Fee Related ES2185493B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200101545A ES2185493B1 (es) 2001-07-03 2001-07-03 Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.
PCT/ES2002/000327 WO2003005301A1 (es) 2001-07-03 2002-07-01 Método para evaluar in vitro en condiciones fisiológicas o patológicas relevantes la actividad biológica de compuestos a gran escala

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101545A ES2185493B1 (es) 2001-07-03 2001-07-03 Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.

Publications (2)

Publication Number Publication Date
ES2185493A1 true ES2185493A1 (es) 2003-04-16
ES2185493B1 ES2185493B1 (es) 2004-06-16

Family

ID=8498272

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101545A Expired - Fee Related ES2185493B1 (es) 2001-07-03 2001-07-03 Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.

Country Status (2)

Country Link
ES (1) ES2185493B1 (es)
WO (1) WO2003005301A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2488351C2 (ru) 2007-11-20 2013-07-27 Конинклейке Филипс Электроникс Н.В. Визуализация васкуляризации

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023968A1 (en) * 1994-03-04 1995-09-08 The Australian National University In vitro angiogenesis assay
WO1999017116A1 (en) * 1997-10-01 1999-04-08 Biocure Limited A multicellular in vitro assay of angiogenesis
WO2000072258A2 (en) * 1999-05-24 2000-11-30 Cellomics, Inc. Method and system for general purpose analysis of experimental data
WO2001042786A2 (en) * 1999-12-09 2001-06-14 Cellomics, Inc. System for cell based screening : cell spreading

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002020729A2 (en) * 2000-09-06 2002-03-14 UNIVERSITé LAVAL In vitro human angiogenesis model

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023968A1 (en) * 1994-03-04 1995-09-08 The Australian National University In vitro angiogenesis assay
WO1999017116A1 (en) * 1997-10-01 1999-04-08 Biocure Limited A multicellular in vitro assay of angiogenesis
WO2000072258A2 (en) * 1999-05-24 2000-11-30 Cellomics, Inc. Method and system for general purpose analysis of experimental data
WO2001042786A2 (en) * 1999-12-09 2001-06-14 Cellomics, Inc. System for cell based screening : cell spreading

Also Published As

Publication number Publication date
ES2185493B1 (es) 2004-06-16
WO2003005301A1 (es) 2003-01-16

Similar Documents

Publication Publication Date Title
JP7253014B2 (ja) 試料に対する光学測定の実施
JP5069245B2 (ja) 非染色核の微速度細胞周期解析
JP2022023070A (ja) 身体試料中の実体を検出する方法および装置
US20200098105A1 (en) Methods and Systems for Assessing Cell Morphology
WO2003019141A3 (en) Analysis of circulating tumor cells, fragments, and debris
WO2003106157A3 (en) AUTOMATIC BLADE COLORING APPARATUS
EP1862533A1 (en) Cell culture estimating system, method of cell culture estimation and cell culture estimating program
DE69627183D1 (de) Verfahren zur automatischen bildanalyse biologischer proben
Salvatore News and Views Methods, marks, and models for inferring hominid and carnivore behavior
WO2007110820A3 (en) Process and system for automatically recognising preneoplastic abnormalities in anatomical structures, and corresponding computer program
IL124814A (en) System and method for imaging and analysis of the movement of individual red blood corpuscles
Brown et al. Tube-forming assays
Meunier et al. An open-access computer image analysis (CIA) method to predict meat and fat content from an android smartphone-derived picture of the bovine 5th-6th rib
KR20200085752A (ko) 생세포 시각화 및 분석
ES2185493A1 (es) Metodo para evaluar in vitro en condiciones fisiologicas o patologicas relevantes la actividad biologica de compuestos a gran escala.
Deckers et al. High-throughput image-based monitoring of cell aggregation and microspheroid formation
Skarpeid et al. Detection of mechanically recovered meat and head meat from cattle in ground beef mixtures by multivariate analysis of isoelectric focusing protein profiles
ATE388460T1 (de) Verfahren und system zur erkennung eines körpers in einer zone in der nähe einer grenzfläche
EP3617916A1 (en) Integration system and integration method
EP3428262B1 (en) Image processing device
Abdullah et al. Automated haversian canal detection for histological sex determination
Schotte et al. Image analysis pipeline for segmentation of a biological porosity network, the lacuno-canalicular system in stingray tesserae
JP2004061403A (ja) 細胞画像解析装置および細胞画像解析方法
HK1049204A1 (en) Cytological imaging system and method
Dirvanauskas et al. Embryo Spatial Model Reconstruction

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20030416

Kind code of ref document: A1

FG2A Definitive protection

Ref document number: 2185493B1

Country of ref document: ES

FD2A Announcement of lapse in spain

Effective date: 20180807