ES2183166T3 - Ensayo de cribado por triple hibrido. - Google Patents

Ensayo de cribado por triple hibrido.

Info

Publication number
ES2183166T3
ES2183166T3 ES97921370T ES97921370T ES2183166T3 ES 2183166 T3 ES2183166 T3 ES 2183166T3 ES 97921370 T ES97921370 T ES 97921370T ES 97921370 T ES97921370 T ES 97921370T ES 2183166 T3 ES2183166 T3 ES 2183166T3
Authority
ES
Spain
Prior art keywords
molecula
binding
hybrid
expression
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97921370T
Other languages
English (en)
Inventor
Jun Liu
Edward J Licitra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Application granted granted Critical
Publication of ES2183166T3 publication Critical patent/ES2183166T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C07K2319/715—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PROPORCIONAN UN PROCEDIMIENTO Y KIT PARA IDENTIFICAR Y CARACTERIZAR UN COMPONENTE CELULAR O MOLECULA PEQUEÑA. EL PROCEDIMIENTO Y KIT INCLUYEN UNA MOLECULA HIBRIDA DE LIGANDO (7) FORMADA POR DOS LIGANDOS, DE MANERA QUE UNO DE ELLOS SEA LA MOLECULA PEQUEÑA (7B) CUYA ESPECIFICIDAD SE DESEA DETERMINAR, MIENTRAS QUE EL OTRO LIGANDO TIENE ESPECIFICIDAD PARA UN BLANCO PREDETERMINADO (7A). EL LIGANDO HIBRIDO SE INTRODUCE EN UN ENTORNO QUE CONTIENE: UN PRIMER VECTOR DE EXPRESION QUE CODIFICA UN BLANCO PARA UNO DE LOS LIGANDOS VINCULADOS A UNA SECUENCIA DE CODIFICACION PARA UN PRIMER MODULO TRANSCRIPCIONAL (8); Y UN SEGUNDO VECTOR DE EXPRESION QUE INCLUYE UN BLANCO PARA EL OTRO LIGANDO VINCULADO A UNA SECUENCIA DE CODIFICACION DE UN SEGUNDO MODULO TRANSCRIPCIONAL (9). EL ENTORNO CONTIENE ADEMAS UN TERCER VECTOR (6) QUE INCLUYE UN GEN INFORMADOR, EN EL QUE LA EXPRESION DEL GEN REPORTER (5) DEPENDE DE LA PROXIMIDAD DE LOS MODULOS TRANSCRIPCIONALES PRIMERO Y SEGUNDO, QUE QUEDA FACILITADA POR LA FIJACION DE AMBOS LIGANDOS DE LA MOLECULA HIBRIDA DE LIGANDOS A SUS BLANCOS.
ES97921370T 1996-04-26 1997-04-25 Ensayo de cribado por triple hibrido. Expired - Lifetime ES2183166T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1734196P 1996-04-26 1996-04-26

Publications (1)

Publication Number Publication Date
ES2183166T3 true ES2183166T3 (es) 2003-03-16

Family

ID=21782024

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97921370T Expired - Lifetime ES2183166T3 (es) 1996-04-26 1997-04-25 Ensayo de cribado por triple hibrido.

Country Status (8)

Country Link
US (1) US5928868A (es)
EP (1) EP0907750B1 (es)
JP (1) JP2000508923A (es)
AT (1) ATE224454T1 (es)
CA (1) CA2252886C (es)
DE (1) DE69715621T2 (es)
ES (1) ES2183166T3 (es)
WO (1) WO1997041255A1 (es)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972193B1 (en) * 1993-02-12 2005-12-06 Board Of Trustees Of Leland Stanford Junior University Regulated transcription of targeted genes and other biological events
US6891021B2 (en) * 1993-02-12 2005-05-10 Board Of Trustees Of The Leland Stanford Junior University Regulated apoptosis
US6841347B1 (en) * 1998-02-05 2005-01-11 The General Hospital Corporation In vivo construction of DNA libraries
US6187535B1 (en) 1998-02-18 2001-02-13 Institut Pasteur Fast and exhaustive method for selecting a prey polypeptide interacting with a bait polypeptide of interest: application to the construction of maps of interactors polypeptides
WO1999067639A1 (en) * 1998-06-25 1999-12-29 Caliper Technologies Corporation High throughput methods, systems and apparatus for performing cell based screening assays
CA2338247A1 (en) * 1998-07-28 2000-02-10 Vimal D. Mehta General screening method for ligand-protein interactions
EP1792989A1 (en) 1999-04-12 2007-06-06 Agensys, Inc. 13 Transmembrane protein expressed in prostate cancer
EP2365081A3 (en) 1999-04-12 2012-07-18 Agensys, Inc. 13 Transmembrane protein expressed in prostate cancer
US6602501B1 (en) 1999-08-12 2003-08-05 Agensys, Inc. C-type lectin transmembrane antigen expressed in human prostate cancer and uses thereof
ES2316387T3 (es) 1999-10-05 2009-04-16 Agensys, Inc. Receptor acoplado a proteina g sobrexpresado en cancer de prostata y la utilizacion del mismo.
US6893818B1 (en) * 1999-10-28 2005-05-17 Agensys, Inc. Gene upregulated in cancers of the prostate
WO2001049874A1 (en) 2000-01-06 2001-07-12 Caliper Technologies Corp. Methods and systems for monitoring intracellular binding reactions
US20020004202A1 (en) * 2000-01-24 2002-01-10 Cornish Virginia W. In vivo screen using chemical inducers of dimerization
US6410246B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
US6410271B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast
US6406863B1 (en) 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
JP2004511219A (ja) 2000-07-12 2004-04-15 アジェンシス，インコーポレイテッド 膀胱癌、卵巣癌、肺癌、および腎臓癌の診断および治療に有用な腫瘍抗原
AU2001286699A1 (en) 2000-08-22 2002-03-04 Agensys, Inc. Nucleic acid and corresponding protein named 158p1h4 useful in the treatment and detection of bladder and other cancers
PT1313850E (pt) 2000-08-28 2008-11-18 Agensys Inc Ácido nucleico e proteína correspondente denominados 85p1b3 úteis no tratamento e na detecção de cancro
HRP20010018A2 (en) 2001-01-09 2002-12-31 Pliva D D Novel anti-inflammatory compounds
US20020168737A1 (en) * 2001-01-24 2002-11-14 Cornish Virginia W. Binding and catalysis screen for high throughput determination of protein function using chemical inducers of dimerization
CA2439263C (en) * 2001-03-02 2012-10-23 Frank Becker Three hybrid assay system
US6924358B2 (en) 2001-03-05 2005-08-02 Agensys, Inc. 121P1F1: a tissue specific protein highly expressed in various cancers
US7271240B2 (en) 2001-03-14 2007-09-18 Agensys, Inc. 125P5C8: a tissue specific protein highly expressed in various cancers
US20030191073A1 (en) 2001-11-07 2003-10-09 Challita-Eid Pia M. Nucleic acid and corresponding protein entitled 161P2F10B useful in treatment and detection of cancer
EP2280030A3 (en) 2001-04-10 2011-06-15 Agensys, Inc. Nucleic acids and corresponding proteins useful in the detection and treatment of various cancers
AU2002256347A1 (en) * 2001-04-20 2002-11-05 President And Fellows Of Harvard College Compositions and methods for the identification of protein interactions in vertebrate cells
JP2005505271A (ja) 2001-09-06 2005-02-24 アジェンシス， インコーポレイテッド 癌の処置および検出において有用なｓｔｅａｐ−１と名称が与えられる核酸および対応するタンパク質
WO2003046198A2 (de) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Verfahren zur selektion und identifikation von peptid- oder proteinmolekülen mittels phage display
DE10220593A1 (de) * 2001-11-28 2003-06-12 Graffinity Pharmaceuticals Ag SPR-Sensorflächenträger
EP1451558A1 (de) * 2001-11-28 2004-09-01 Graffinity Pharmaceuticals Aktiengesellschaft Spr-sensorflächenträger
US20030138785A1 (en) * 2001-12-21 2003-07-24 Stephan Kopytek In vivo protein screen based on enzyme-assisted chemically induced dimerization ("CID")
US20060069047A1 (en) * 2002-02-15 2006-03-30 Michael Burnet Antibiotic conjugates
US20040186063A1 (en) * 2002-02-15 2004-09-23 Hans-Jurgen Gutke Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof
WO2003070174A2 (en) * 2002-02-15 2003-08-28 Sympore Gmbh Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof
US20040005641A1 (en) * 2002-02-15 2004-01-08 Michael Burnet Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof
US7083918B2 (en) * 2002-04-24 2006-08-01 The Trustees Of Columbia University In The City Of New York Bacterial small-molecule three-hybrid system
WO2004005313A2 (en) 2002-07-08 2004-01-15 Pliva - Istrazivacki Institut D.O.O. Hybrid molecules of macrolides with steroid/non-steroid anti-inflammatory, antineoplastic and antiviral active molecules
PL374731A1 (en) * 2002-07-08 2005-10-31 Pliva-Istrazivacki Institut D.O.O. New compounds, compositions and methods for treatment of inflammatory diseases and conditions
CA2489398A1 (en) 2002-07-08 2004-01-15 Pliva-Istrazivacki Institut D.O.O. Novel nonsteroidal anti-inflammatory substances, compositions and methods for their use
EP1539786A4 (en) * 2002-07-10 2006-09-06 Stratagene California HUMANIZED, GREEN FLUORESCENT RENILLA RENIFORMIS PROTEIN AS FRUIT
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2004016733A2 (en) 2002-08-16 2004-02-26 Agensys, Inc. Nucleic acid and corresponding protein entitled 251p5g2 useful in treatment and detection of cancer
CA2515699C (en) 2003-02-10 2015-01-27 Aya Jakobovits Nucleic acid and corresponding protein named 158p1d7 useful in the treatment and detection of bladder and other cancers
EP2319524B1 (en) 2003-05-30 2013-08-21 Agensys, Inc. Prostate stem cell antigen (PSCA) variants and subsequences thereof
WO2005118864A2 (en) 2004-05-28 2005-12-15 Agensys, Inc. Antibodies and related molecules that bind to psca proteins
US8034553B2 (en) 2004-06-24 2011-10-11 Kimberly-Clark Worldwide, Inc. Biomarkers for wound healing
EP1773336A1 (en) * 2004-07-16 2007-04-18 Novartis AG Use of a steroid for enhancement of skin permeability
US20090011951A1 (en) * 2004-10-29 2009-01-08 Eisai R&D Management Co., Ltd. Method of Gene Screening With Yeast Having Ergosterol Synthase Undergoing Inducible Expression
EP1858916B1 (en) 2005-03-15 2014-07-09 Fujirebio Europe N.V. Hepatitis-b viral variants with reduced susceptibility to nucleoside analogs and uses thereof
EP2444099A1 (en) 2005-03-31 2012-04-25 Agensys, Inc. Antibodies and related molecules that bind to 161P2F10B proteins
CA2615581A1 (en) * 2005-07-26 2007-02-01 Merckle Gmbh Macrolide conjugates of pyrrolizine and indolizine compounds as inhibitors of 5-lipooxygenase and cyclooxygenase
US20100009348A1 (en) * 2005-09-28 2010-01-14 Attagene, Inc. Methods and Constructs for Analyzing Biological Activities of Biological Specimens and Determining States of Organism
WO2007042771A1 (en) * 2005-10-10 2007-04-19 Astrazeneca Ab Three hybrid screening assay using reverse transfected mammalian cell arrays
CA3187687A1 (en) 2007-09-14 2009-03-19 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20090162858A1 (en) * 2007-09-18 2009-06-25 Cornish Virginia W Orthogonal chemical inducer of dimerization
CN101131363B (zh) * 2007-09-30 2010-12-01 南京大学 一种基于酵母三杂交的小分子化合物检测方法
KR102318383B1 (ko) 2010-07-16 2021-10-27 아디맵 엘엘씨 항체 라이브러리

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991004321A1 (en) * 1989-09-25 1991-04-04 President And Fellows Of Harvard College Receptor for fk-506
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5115098A (en) * 1990-02-28 1992-05-19 President And Fellows Of Harvard College End-blocked peptides inhibiting binding capacity of gp120
US5362629A (en) * 1991-08-05 1994-11-08 President And Fellows Of Harvard College Detection of immunosuppressants
US5498597A (en) * 1992-01-17 1996-03-12 Dana-Farber Cancer Institute, Inc. FKBP-13, an FK506-binding immunophilin
ATE453709T1 (de) * 1993-02-12 2010-01-15 Univ Leland Stanford Junior Regulierte transkription von zielgerichteten genen und andere biologische ereignisse
JPH08510115A (ja) * 1993-03-31 1996-10-29 カダス ファーマシューティカルズ、インコーポレーテッド フェロモン系タンパク質代用物を産生するように操作された酵母細胞、ならびにその利用法
HUT73100A (en) * 1993-07-16 1996-06-28 Harvard College Regulated apoptosis
AU2159895A (en) * 1994-03-11 1995-09-25 Ariad Pharmaceuticals, Inc. Methods and materials for identifying inhibitors of molecular interactions mediated by sh3 domains
WO1995030012A1 (en) * 1994-04-26 1995-11-09 Cadus Pharmaceutical Corporation Functional expression of mammalian adenylyl cyclase in yeast
US5989808A (en) * 1994-06-14 1999-11-23 American Cyanamid Company Identification of compounds affecting specific interaction of peptide binding pairs
ATE254136T1 (de) * 1994-07-20 2003-11-15 Gen Hospital Corp Interaktions-fullensysteme zum nachweis von protein-interaktionen
ES2245781T3 (es) * 1994-08-18 2006-01-16 Ariad Gene Therapeutics, Inc. Nuevos agentes de multimerizacion.
EP0776335A4 (en) * 1994-08-18 1999-09-15 Ariad Gene Therapeutics Inc REGULATABLE ELIMINATION OF GENE EXPRESSION, GENE PRODUCT FUNCTION AND GENE TECHNOLOGICALLY MANUFACTURED CELLS
ATE307899T1 (de) * 1994-11-01 2005-11-15 Ariad Gene Therapeutics Inc Neue methode zum auffindenund bewerten biologisch aktiver moleküle
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions

Also Published As

Publication number Publication date
CA2252886C (en) 2008-02-12
WO1997041255A1 (en) 1997-11-06
US5928868A (en) 1999-07-27
ATE224454T1 (de) 2002-10-15
JP2000508923A (ja) 2000-07-18
EP0907750B1 (en) 2002-09-18
DE69715621D1 (de) 2002-10-24
DE69715621T2 (de) 2003-05-22
CA2252886A1 (en) 1997-11-06
EP0907750A1 (en) 1999-04-14

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