ES2180263T3 - Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. - Google Patents

Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Info

Publication number
ES2180263T3
ES2180263T3 ES99660099T ES99660099T ES2180263T3 ES 2180263 T3 ES2180263 T3 ES 2180263T3 ES 99660099 T ES99660099 T ES 99660099T ES 99660099 T ES99660099 T ES 99660099T ES 2180263 T3 ES2180263 T3 ES 2180263T3
Authority
ES
Spain
Prior art keywords
energy
luminescent
energy transfer
homogeneum
luminiscent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99660099T
Other languages
English (en)
Inventor
Martti Latva
Ilkka Hemmila
Kaj Blomberg
Pertti Hurskainen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wallac Oy
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Application granted granted Critical
Publication of ES2180263T3 publication Critical patent/ES2180263T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La invención es un ensayo de bioafinidad a base de transferencia de energía luminiscente que comprende un primer grupo marcado con un compuesto de donación de energía (donante) y un segundo grupo de aceptación de energía (receptor) , donde el donante es un quelato de lantánido luminiscente que tiene una larga duración de vida en estado excitado y el receptor es o un compuesto luminiscente que tiene una corta duración de vida en estado excitado o un compuesto no luminiscente. Se mide el incremento o la reducción, respectivamente de la transferencia de energía del donante a receptor, resultante del acortamiento o del alargamiento respectivamente, de la distancia entre dichos grupos.
ES99660099T 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. Expired - Lifetime ES2180263T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/116,763 US5998146A (en) 1998-07-17 1998-07-17 Homogeneous luminescence assay method based on energy transfer

Publications (1)

Publication Number Publication Date
ES2180263T3 true ES2180263T3 (es) 2003-02-01

Family

ID=22369056

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99660099T Expired - Lifetime ES2180263T3 (es) 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Country Status (4)

Country Link
US (1) US5998146A (es)
EP (1) EP0973036B1 (es)
DE (1) DE69902724T2 (es)
ES (1) ES2180263T3 (es)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
JP2003527833A (ja) 1999-10-14 2003-09-24 クロンテック・ラボラトリーズ・インコーポレーテッド 花虫類に由来する発色団／蛍光体、およびそれらの使用法
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
US6844154B2 (en) * 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
ATE300050T1 (de) * 2000-11-16 2005-08-15 Hoffmann La Roche Farbstoffpaare für fluoreszenz-resonanz-energie- transfer (fret) messungen
WO2003003063A2 (en) * 2001-06-28 2003-01-09 Ia, Inc. Fiber-optic sensor array
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US6760104B2 (en) 2001-07-20 2004-07-06 Grigoriy Gomelskiy Apparatus, method, and system for analyzing samples using triboluminescent technology
DE10137530A1 (de) * 2001-08-01 2003-02-13 Presens Prec Sensing Gmbh Anordnung und Verfahren zur Mehrfach-Fluoreszenzmessung
DE10153829A1 (de) 2001-11-05 2003-05-28 Bayer Ag Assay basierend auf dotierten Nanoteilchen
DE10159579A1 (de) * 2001-12-05 2003-06-18 Applera Deutschland Gmbh Heterogenes Lumineszenz-Nachweisverfahren in Kavitätenstrukturen
WO2003054158A2 (en) * 2001-12-19 2003-07-03 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
WO2003062824A1 (en) * 2002-01-23 2003-07-31 Boditech Inc. Lateral flow quantitative assay method and strip and laser-induced fluoerescence detection device therefor
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20030232394A1 (en) * 2002-06-12 2003-12-18 Aventis Pharma Deutschland Gmbh High throughput screening method and assay system for determining the interaction between C-reactive protein and components binding to it
RU2221236C1 (ru) * 2002-09-16 2004-01-10 Калачев Алексей Александрович Способ анализа физических и/или химических свойств поверхностного слоя твердого тела (варианты)
US7951923B2 (en) 2002-11-12 2011-05-31 Zakrytoe Aktsionernoe Obschestvo ‘Evrogen’ Fluorescent proteins and chromoproteins from non-Aequorea hydrozoa species and methods for using same
DE10259677B4 (de) * 2002-12-18 2008-01-24 Büddefeld, Jürgen, Prof. Dr.-Ing. Sonden zum Nachweis von Nukleinsäuren, insbesondere zum Nachweis pathogener Keime in Lebensmitteln
ATE474922T1 (de) 2002-12-26 2010-08-15 Zakrytoe Aktsionernoe Obschest Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
FI20030460A0 (fi) * 2003-03-28 2003-03-28 Tero Soukka Homogeeninen määritysmenetelmä, joka perustuu luminesenssienergiasiirtoon
GB0311948D0 (en) * 2003-05-23 2003-06-25 Stockport Innovation Ltd Light emitting probes
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2541075C (en) * 2003-10-01 2010-04-27 Wallac Oy Homogeneous time-resolved energy transfer assay
EP1732944B1 (en) * 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
US20060147954A1 (en) * 2004-10-19 2006-07-06 Wallac Oy Novel probe and its use in bioaffinity assays
DE112005002636T5 (de) * 2004-10-22 2007-09-06 Wallac Oy Hochempfindliche homogene Analyse auf der Grundlage einer FRET-Messung mit Anti-Stokes-Verschiebung
ES2399563T3 (es) * 2005-11-04 2013-04-02 Evrogen, Jsc Proteínas verdes fluorescentes modificadas y método para la utilización de las mismas
WO2007085923A2 (en) 2006-01-25 2007-08-02 Evrogen, Ip Novel fluorescent proteins and methods for using same
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
US8557601B2 (en) 2006-07-10 2013-10-15 The Regents Of The University Of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
US8173800B2 (en) 2006-08-15 2012-05-08 The Regents Of The University Of California Luminescent macrocyclic lanthanide complexes
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
WO2008064016A2 (en) * 2006-11-13 2008-05-29 Perkinelmer Las, Inc. Detecting molecular interactions
US8507199B2 (en) 2007-01-25 2013-08-13 Lumiphore, Inc. Multi-color time resolved fluorophores based on macrocyclic lanthanide complexes
JP5545814B2 (ja) * 2007-06-18 2014-07-09 中外製薬株式会社 抗体依存性細胞障害活性を模擬する抗体の結合活性測定法
WO2009059305A2 (en) * 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
FI20070926A0 (fi) * 2007-11-30 2007-11-30 Wallac Oy Transportterimääritys
US20090269731A1 (en) * 2008-04-29 2009-10-29 Burnham Institute For Medical Research E3-independent ubiquitinylation assay
DE202008009713U1 (de) * 2008-06-26 2009-08-06 InBio Prof. Jürgen Büddefeld Dr. Peter Klauth Prof. Manfred Rietz GbR (vertretungsberechtigter Gesellschafter: Dr. Peter Klauth, 41189 Mönchengladbach) Abstandsgesteuerte Energietransfer-Farbstoffkomplexe
WO2011025790A1 (en) 2009-08-24 2011-03-03 Lumiphore, Inc. Macrocyclic hopo chelators
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
JP2013515744A (ja) * 2009-12-24 2013-05-09 ルミフォア，インコーポレイテッド 放射性医薬品錯体
US8834846B2 (en) 2010-05-06 2014-09-16 Bruker Biospin Corporation Fluorescent NIRF activatable probes for disease detection
WO2012017194A1 (en) * 2010-07-31 2012-02-09 Advanced Biomedical Limited Method, reagent, and apparatus for detecting a chemical chelator
JP6043288B2 (ja) 2010-09-15 2016-12-14 エンダセア， インコーポレイテッド 時間分解蛍光ベースのアッセイによるリポ多糖の測定のための使用方法およびキット
US11453652B2 (en) 2013-03-15 2022-09-27 Lumiphore, Inc. Di-macrocycles
CN104181310A (zh) * 2014-07-30 2014-12-03 美艾利尔（上海）诊断产品有限公司 一种检测β-hCG含量的时间分辨免疫荧光试剂盒及其制备方法和应用
CN107810420B (zh) 2015-06-25 2020-08-14 豪夫迈·罗氏有限公司 用于测定抗体或配体结合和功能的基于细胞的测定
EP3559666A1 (en) 2016-12-21 2019-10-30 H. Hoffnabb-La Roche Ag Assay for determining antibody or ligand binding and function
FI130554B (en) 2020-01-20 2023-11-16 Oy Arctic Partners Ab Luminescence hybridisation assay method
CN112946272B (zh) * 2021-01-28 2021-12-24 无锡壹闪生物科技有限公司 一种核基质蛋白22的快速检测试剂盒及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4594179A (en) * 1984-08-01 1986-06-10 The United States Of America As Represented By The United States Department Of Energy Reduction of reabsorption effects in scintillators by employing solutes with large Stokes shifts
US4822733A (en) * 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US5279943A (en) * 1985-08-02 1994-01-18 Compagnie Oris Industrie Homogeneous process for the detection and/or determination by luminescence of an analyte in a medium in which it may be present
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
DE3789430T2 (de) * 1986-06-17 1994-10-27 Baxter Diagnostics Inc Homogenes fluortestverfahren mit abstoss des fluorzenten hintergrundes.
GB8820887D0 (en) * 1988-09-06 1988-10-05 Atomic Energy Authority Uk Detecting selected species
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
FR2664699B1 (fr) * 1990-07-13 1995-08-18 Cis Bio Int Procede d'amplification du signal d'emission d'un compose luminescent.
EP0552108B1 (en) * 1992-01-17 1999-11-10 Lakowicz, Joseph R. Energy transfer phase-modulation fluoro-immunoassay
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer
FI963989L (fi) * 1996-10-04 1998-04-05 Wallac Oy Homogeenisiä määritysmenetelmiä, jotka perustuvat luminesenssienergiasiirtoon

Also Published As

Publication number Publication date
EP0973036A2 (en) 2000-01-19
EP0973036A3 (en) 2000-03-08
EP0973036B1 (en) 2002-09-04
US5998146A (en) 1999-12-07
DE69902724D1 (de) 2002-10-10
DE69902724T2 (de) 2003-01-09

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