ES2180263T3 - Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. - Google Patents

Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Info

Publication number
ES2180263T3
ES2180263T3 ES99660099T ES99660099T ES2180263T3 ES 2180263 T3 ES2180263 T3 ES 2180263T3 ES 99660099 T ES99660099 T ES 99660099T ES 99660099 T ES99660099 T ES 99660099T ES 2180263 T3 ES2180263 T3 ES 2180263T3
Authority
ES
Spain
Prior art keywords
energy
luminescent
energy transfer
homogeneum
luminiscent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99660099T
Other languages
English (en)
Inventor
Martti Latva
Ilkka Hemmila
Kaj Blomberg
Pertti Hurskainen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wallac Oy
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Application granted granted Critical
Publication of ES2180263T3 publication Critical patent/ES2180263T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención es un ensayo de bioafinidad a base de transferencia de energía luminiscente que comprende un primer grupo marcado con un compuesto de donación de energía (donante) y un segundo grupo de aceptación de energía (receptor) , donde el donante es un quelato de lantánido luminiscente que tiene una larga duración de vida en estado excitado y el receptor es o un compuesto luminiscente que tiene una corta duración de vida en estado excitado o un compuesto no luminiscente. Se mide el incremento o la reducción, respectivamente de la transferencia de energía del donante a receptor, resultante del acortamiento o del alargamiento respectivamente, de la distancia entre dichos grupos.
ES99660099T 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia. Expired - Lifetime ES2180263T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/116,763 US5998146A (en) 1998-07-17 1998-07-17 Homogeneous luminescence assay method based on energy transfer

Publications (1)

Publication Number Publication Date
ES2180263T3 true ES2180263T3 (es) 2003-02-01

Family

ID=22369056

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99660099T Expired - Lifetime ES2180263T3 (es) 1998-07-17 1999-06-03 Metodo de ensayo luminiscente homogeneo basado en transferencia de energia.

Country Status (4)

Country Link
US (1) US5998146A (es)
EP (1) EP0973036B1 (es)
DE (1) DE69902724T2 (es)
ES (1) ES2180263T3 (es)

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DE10159579A1 (de) * 2001-12-05 2003-06-18 Applera Deutschland Gmbh Heterogenes Lumineszenz-Nachweisverfahren in Kavitätenstrukturen
EP1456223B1 (en) * 2001-12-19 2009-08-12 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
WO2003062824A1 (en) * 2002-01-23 2003-07-31 Boditech Inc. Lateral flow quantitative assay method and strip and laser-induced fluoerescence detection device therefor
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DE10259677B4 (de) * 2002-12-18 2008-01-24 Büddefeld, Jürgen, Prof. Dr.-Ing. Sonden zum Nachweis von Nukleinsäuren, insbesondere zum Nachweis pathogener Keime in Lebensmitteln
DK1576157T3 (da) 2002-12-26 2010-11-01 Zakrytoe Aktsionernoe Obschest Fluorescerende proteiner fra Copepode-arter og fremgangsmåder til anvendelse af samme
FI20030460A0 (fi) * 2003-03-28 2003-03-28 Tero Soukka Homogeeninen määritysmenetelmä, joka perustuu luminesenssienergiasiirtoon
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EP1732944B1 (en) * 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
US20060147954A1 (en) * 2004-10-19 2006-07-06 Wallac Oy Novel probe and its use in bioaffinity assays
DE112005002636T5 (de) * 2004-10-22 2007-09-06 Wallac Oy Hochempfindliche homogene Analyse auf der Grundlage einer FRET-Messung mit Anti-Stokes-Verschiebung
US7417131B2 (en) * 2005-11-04 2008-08-26 Evrogen Joint Stock Company Modified green fluorescent proteins and methods for using same
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
DE602007009498D1 (de) * 2006-01-25 2010-11-11 Evrogen Ip Neue fluoreszenzproteine und verfahren zur verwendung davon
CA2659251C (en) 2006-07-10 2016-06-14 The Regents Of The University Of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
WO2008063721A2 (en) 2006-08-15 2008-05-29 The Regents Of The University Of California Luminescent macrocyclic lanthanide complexes
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WO2009059305A2 (en) * 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
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US20090269731A1 (en) * 2008-04-29 2009-10-29 Burnham Institute For Medical Research E3-independent ubiquitinylation assay
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CN104181310A (zh) * 2014-07-30 2014-12-03 美艾利尔（上海）诊断产品有限公司 一种检测β-hCG含量的时间分辨免疫荧光试剂盒及其制备方法和应用
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Also Published As

Publication number Publication date
DE69902724T2 (de) 2003-01-09
EP0973036A2 (en) 2000-01-19
DE69902724D1 (de) 2002-10-10
EP0973036B1 (en) 2002-09-04
EP0973036A3 (en) 2000-03-08
US5998146A (en) 1999-12-07

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