ES2179134T3 - Analizador de particulas fluorescentes con alineamiento de sincronizacion para la sustraccion analogica de impulsos procedentes de impulsos fluorescentes de diferentes emplazamientos de excitacion. - Google Patents

Analizador de particulas fluorescentes con alineamiento de sincronizacion para la sustraccion analogica de impulsos procedentes de impulsos fluorescentes de diferentes emplazamientos de excitacion.

Info

Publication number
ES2179134T3
ES2179134T3 ES96105049T ES96105049T ES2179134T3 ES 2179134 T3 ES2179134 T3 ES 2179134T3 ES 96105049 T ES96105049 T ES 96105049T ES 96105049 T ES96105049 T ES 96105049T ES 2179134 T3 ES2179134 T3 ES 2179134T3
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ES
Spain
Prior art keywords
location
delay
impulse
red
apc
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES96105049T
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English (en)
Inventor
Michael A Hoffman
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of ES2179134T3 publication Critical patent/ES2179134T3/es
Anticipated expiration legal-status Critical
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1438—Using two lasers in succession

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

UN SISTEMA DE CITOMETRIA (10) QUE INCLUYE UN TUBO DE FLUJO (14) A TRAVES DEL CUAL PASAN PARTICULAS MARCADAS CON DISTINTOS FLUOROCROMOS DESDE UNA PRIMERA LOCALIZACION (LC1), ILUMINADA POR UN LASER ROJO (LZ1), A UNA SEGUNDA LOCALIZACION (LC2), ILUMINADA POR UN LASER AZUL (LZ2). UN FOTODETECTOR "ROJO" (PDR) ESTA OPTICAMENTE UNIDO PARA DETECTAR FLUORESCENCIA ROJA DE LAS DOS LOCALIZACIONES. FOTODETECTORES "AMARILLO" (PDY) Y "VERDE" (PDG) DETECTAN RESPECTIVAMENTE FLUORESCENCIA AMARILLA Y VERDE DE LA SEGUNDA LOCALIZACION, MIENTRAS UN DETECTOR DE "LUZ DIFUSA" (PDS) DETECTA LA LUZ DIFUSA DE LA SEGUNDA LOCALIZACION. DURANTE UNA PRUEBA, EL FOTODETECTOR ROJO PUEDE ENVIAR IMPULSOS QUE CORRESPONDEN PRINCIPALMENTE A FLUOROCROMOS APC EN LA PRIMERA LOCALIZACION Y A PERCP EN LA SEGUNDA LOCALIZACION RESPECTIVAMENTE. UN DISPOSITIVO DE RETARDO (30) RETARDA EL IMPULSO APC RELATIVO AL IMPULSO PERCP DE MANERA QUE LAS CIMAS PUEDEN SER COLOCADAS EN ESCALAS Y SUBSTRAIDAS EN EL DOMINIO ELECTRICO ANALOGO PARA ELIMINARLA INTERMODULACION APC/PERCP. EL RETARDO SE CALIBRA UTILIZANDO UN CONJUNTO DE CELULAS ETIQUETADAS APC. CADA UNA DE ESTAS CELULAS GENERA UN IMPULSO ELECTRICO DE FLUORESCENCIA ROJA EN LA PRIMERA LOCALIZACION Y UN IMPULSO DE LUZ DIFUSA EN LA SEGUNDA LOCALIZACION. EL DIFERENCIAL DE TIEMPO MEDIO ENTRE ESTOS DOS IMPULSOS PARA EL CONJUNTO DE CELULAS DE TIEMPO DETERMINA EL RETARDO OBJETIVO. ESTE SE COMPARA CON EL RETARDO REAL IMPUESTO POR EL DISPOSITIVO DE RETARDO. SI EL ERROR ES MAYOR A 0.5 {MI}S, EL RETARDO IMPUESTO PUEDE AJUSTARSE HASTA QUE EL RETARDO SEA INFERIOR A 0.5 {MI}S, O DENTRO DE LA TOLERANCIA REQUERIDA PARA IMPULSOS DE DIFERENCIA FIABLES A GENERAR POR EL SUBSTRACTOR DE IMPULSO.
ES96105049T 1995-04-06 1996-03-29 Analizador de particulas fluorescentes con alineamiento de sincronizacion para la sustraccion analogica de impulsos procedentes de impulsos fluorescentes de diferentes emplazamientos de excitacion. Expired - Lifetime ES2179134T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/417,744 US5682038A (en) 1995-04-06 1995-04-06 Fluorescent-particle analyzer with timing alignment for analog pulse subtraction of fluorescent pulses arising from different excitation locations

Publications (1)

Publication Number Publication Date
ES2179134T3 true ES2179134T3 (es) 2003-01-16

Family

ID=23655246

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96105049T Expired - Lifetime ES2179134T3 (es) 1995-04-06 1996-03-29 Analizador de particulas fluorescentes con alineamiento de sincronizacion para la sustraccion analogica de impulsos procedentes de impulsos fluorescentes de diferentes emplazamientos de excitacion.

Country Status (4)

Country Link
US (1) US5682038A (es)
EP (1) EP0737855B1 (es)
DE (1) DE69623523T2 (es)
ES (1) ES2179134T3 (es)

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Also Published As

Publication number Publication date
EP0737855A1 (en) 1996-10-16
EP0737855B1 (en) 2002-09-11
DE69623523D1 (de) 2002-10-17
US5682038A (en) 1997-10-28
DE69623523T2 (de) 2003-07-31

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