ES2176117B1 - MEDIUM RESISTANT PLANT WITH HEAVY METALS. - Google Patents

MEDIUM RESISTANT PLANT WITH HEAVY METALS.

Info

Publication number
ES2176117B1
ES2176117B1 ES200100590A ES200100590A ES2176117B1 ES 2176117 B1 ES2176117 B1 ES 2176117B1 ES 200100590 A ES200100590 A ES 200100590A ES 200100590 A ES200100590 A ES 200100590A ES 2176117 B1 ES2176117 B1 ES 2176117B1
Authority
ES
Spain
Prior art keywords
heavy metals
media
contaminated
plant
resistant plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200100590A
Other languages
Spanish (es)
Other versions
ES2176117A1 (en
Inventor
Solis Jose Ramon Dominguez
Martinez Cecilia Gotor
Gonzalez Luis Carlos Romero
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Sevilla
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Sevilla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Universidad de Sevilla filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200100590A priority Critical patent/ES2176117B1/en
Priority to PCT/ES2002/000123 priority patent/WO2002072834A1/en
Publication of ES2176117A1 publication Critical patent/ES2176117A1/en
Application granted granted Critical
Publication of ES2176117B1 publication Critical patent/ES2176117B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8259—Phytoremediation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Soil Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Manufacture And Refinement Of Metals (AREA)
  • Processing Of Solid Wastes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Planta resistente a medios con metales pesados. La presente invención se refiere a una planta con una elevada capacidad de acumular metales pesados en su parte aérea y su empleo en la recuperación de medios contaminados con metales pesados. Las plantas han sido modificadas genéticamente para ser resistentes a medios contaminados por metales pesados y para en esos medios contaminados tener la capacidad de absorber los metales pesados, acumulándolos en sus hojas, para recuperar el medio contaminado. La modificación genética se ha basado en la identificación de genes de plantas implicados en los procesos de detoxificación de metales pesados y la sobreexpresión de los mismos dando lugar a plantas transformadas susceptibles de ser utilizadas en procesos de fitorremediación.Plant resistant to heavy metal media. The present invention relates to a plant with a high capacity to accumulate heavy metals in its aerial part and its use in the recovery of media contaminated with heavy metals. The plants have been genetically modified to be resistant to media contaminated by heavy metals and in those contaminated media to have the ability to absorb heavy metals, accumulating them in their leaves, to recover the contaminated medium. The genetic modification has been based on the identification of plant genes involved in the heavy metal detoxification processes and their overexpression giving rise to transformed plants that can be used in phytoremediation processes.

ES200100590A 2001-03-14 2001-03-14 MEDIUM RESISTANT PLANT WITH HEAVY METALS. Expired - Fee Related ES2176117B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200100590A ES2176117B1 (en) 2001-03-14 2001-03-14 MEDIUM RESISTANT PLANT WITH HEAVY METALS.
PCT/ES2002/000123 WO2002072834A1 (en) 2001-03-14 2002-03-14 Plant that is resistant to heavy metal-contaminated media

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200100590A ES2176117B1 (en) 2001-03-14 2001-03-14 MEDIUM RESISTANT PLANT WITH HEAVY METALS.

Publications (2)

Publication Number Publication Date
ES2176117A1 ES2176117A1 (en) 2002-11-16
ES2176117B1 true ES2176117B1 (en) 2004-02-16

Family

ID=8497073

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200100590A Expired - Fee Related ES2176117B1 (en) 2001-03-14 2001-03-14 MEDIUM RESISTANT PLANT WITH HEAVY METALS.

Country Status (2)

Country Link
ES (1) ES2176117B1 (en)
WO (1) WO2002072834A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010146046A1 (en) * 2009-06-15 2010-12-23 Basf Plant Science Company Gmbh Hydroponic systems for generating transgenic plants
CN104138894A (en) * 2013-05-08 2014-11-12 中国科学院沈阳应用生态研究所 Cadmium-contaminated soil enhanced repairing agent and application
CN111518564A (en) * 2020-06-24 2020-08-11 代彦霞 Material for reducing migration-state lead ions in soil and preparation method thereof
CN113736798B (en) * 2021-07-30 2023-11-28 上海市农业科学院 Zinc-cadmium-resistant gene TmZRC1S from truffle and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6489537B1 (en) * 1998-08-07 2002-12-03 The Trustees Of The University Of Pennsylvania Phytochelatin synthases and uses therefor

Also Published As

Publication number Publication date
ES2176117A1 (en) 2002-11-16
WO2002072834A1 (en) 2002-09-19

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