ES2170006B1 - Sintesis enzimatica del antioxidante hidroxitirosol. - Google Patents

Sintesis enzimatica del antioxidante hidroxitirosol.

Info

Publication number
ES2170006B1
ES2170006B1 ES200002073A ES200002073A ES2170006B1 ES 2170006 B1 ES2170006 B1 ES 2170006B1 ES 200002073 A ES200002073 A ES 200002073A ES 200002073 A ES200002073 A ES 200002073A ES 2170006 B1 ES2170006 B1 ES 2170006B1
Authority
ES
Spain
Prior art keywords
tyrosol
antioxidant
reaction
vitamin
hydroxytyrosol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200002073A
Other languages
English (en)
Other versions
ES2170006A1 (es
Inventor
De Gea Juan Carlos Espin
Rivas Cristina Soler
Harry J Wichers
Barberan Francisco A Tomas
Viguera Cristina Garcia
De Arce Federico Ferreres
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200002073A priority Critical patent/ES2170006B1/es
Priority to PCT/ES2001/000314 priority patent/WO2002016628A1/es
Priority to AU2001282147A priority patent/AU2001282147A1/en
Priority to EP01960746A priority patent/EP1310562A1/en
Publication of ES2170006A1 publication Critical patent/ES2170006A1/es
Priority to US10/364,915 priority patent/US20030180833A1/en
Application granted granted Critical
Publication of ES2170006B1 publication Critical patent/ES2170006B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B5/00—Preserving by using additives, e.g. anti-oxidants
    • C11B5/0021—Preserving by using additives, e.g. anti-oxidants containing oxygen
    • C11B5/0035—Phenols; Their halogenated and aminated derivates, their salts, their esters with carboxylic acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D7/00—Edible oil or fat compositions containing an aqueous phase, e.g. margarines
    • A23D7/005—Edible oil or fat compositions containing an aqueous phase, e.g. margarines characterised by ingredients other than fatty acid triglycerides
    • A23D7/0056—Spread compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Emergency Medicine (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Anti-Oxidant Or Stabilizer Compositions (AREA)

Abstract

Síntesis enzimática del antioxidante hidroxitirosol. La reacción transcurre en un medio tamponado con fosfato en medio acuoso, a pH neutro, y a temperatura ambiente. El medio de reacción, se compone por tanto de: tirosol, como precursor; tirosinasa de champiñón (comercial) para catalizar el proceso y vitamina C en exceso. Tras una agitación inicial, la reacción comienza. Cuando la concentración de tirosol inicial se agote la reacción se para. Para que continúe basta con añadir más tirosol (siempre cuidando que la relación vitamina C/tirosol sea mayor que 1). Cuando se obtenga la concentración de hidroxitirosol deseada, se filtra por una membrana. La enzima, de peso molecular más del doble del tamaño de poro, queda retenida en el filtro, y puede ser de nuevo reutilizada. Este primer extracto muy enriquecido en antioxidante hidroxitirosol, con una alta capacidad antioxidante, (en este caso, en combinación con vitamina C), ya es susceptible de utilizarse como aditivo alimentario.
ES200002073A 2000-08-11 2000-08-11 Sintesis enzimatica del antioxidante hidroxitirosol. Expired - Fee Related ES2170006B1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES200002073A ES2170006B1 (es) 2000-08-11 2000-08-11 Sintesis enzimatica del antioxidante hidroxitirosol.
PCT/ES2001/000314 WO2002016628A1 (es) 2000-08-11 2001-08-02 Síntesis enzimática del antioxidante hidroxitirosol
AU2001282147A AU2001282147A1 (en) 2000-08-11 2001-08-02 Enzymatic synthesis of antioxidant hydroxytyrosol
EP01960746A EP1310562A1 (en) 2000-08-11 2001-08-02 Enzymatic synthesis of antioxidant hydroxytyrosol
US10/364,915 US20030180833A1 (en) 2000-08-11 2003-02-11 Enzymatic synthesis of antioxidant hydroxytyrosol

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200002073A ES2170006B1 (es) 2000-08-11 2000-08-11 Sintesis enzimatica del antioxidante hidroxitirosol.

Publications (2)

Publication Number Publication Date
ES2170006A1 ES2170006A1 (es) 2002-07-16
ES2170006B1 true ES2170006B1 (es) 2003-11-01

Family

ID=8494709

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200002073A Expired - Fee Related ES2170006B1 (es) 2000-08-11 2000-08-11 Sintesis enzimatica del antioxidante hidroxitirosol.

Country Status (5)

Country Link
US (1) US20030180833A1 (es)
EP (1) EP1310562A1 (es)
AU (1) AU2001282147A1 (es)
ES (1) ES2170006B1 (es)
WO (1) WO2002016628A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002229784B2 (en) * 2001-02-15 2006-06-22 Consejo Superior De Investigaciones Cientificas Method for obtaining purified hydroxytyrosol from products and by-products derived from the olive tree
EP1474130A2 (en) 2002-02-08 2004-11-10 Lavipharm S.A. Use of compounds and compositions derived from olives for protecting cells against dna damage
ES2233208B1 (es) * 2003-11-27 2006-10-01 Puleva Biotech, S.A. Metodo para la preparacion enzimatica de compuestos antioxidantes.
ITMI20041627A1 (it) * 2004-08-06 2004-11-06 Lachifarma S R L Lab Chimi Co Processo per il recupero di tirosolo idrossitirosolo e altri componenti fenolici da acque di vegetazione e metodo di ossidazione catalitica di tirosolo a idrossitirosolo
CN101060794B (zh) * 2004-11-16 2012-03-07 帝斯曼知识产权资产管理有限公司 抗氧化剂化合物用于肌肉复原的用途
US20090076142A1 (en) * 2005-05-09 2009-03-19 The Trustees Of The University Of Pennsylvania Use of the irritating principal oleocanthal in olive oil, as well as structurally and functionally similar compounds
EP1922933A1 (de) * 2006-11-14 2008-05-21 DSMIP Assets B.V. Nahrungsmittel enthaltend einen Olivenextrakt
WO2008064835A1 (en) * 2006-11-27 2008-06-05 Dsm Ip Assets B.V. Fermentative production of hydroxytyrosol
ITMI20070519A1 (it) * 2007-03-15 2008-09-16 Maurizio Barontini Procedimento per la preparazione di derivati dell'idrossitirosolo e di idrossitirosolo
WO2010012871A1 (fr) * 2008-07-30 2010-02-04 Institut De Recherche Pour Le Developpement Utilisation d'une hydroxyphenylacetate hydroxylase (hpah) pour produire de l'hydroxytyrosol
ES2395317B1 (es) 2011-07-08 2014-04-16 Consejo Superior De Investigaciones Cientificas (Csic) Procedimiento para la obtención de extracto de hidroxitirosol, extracto mezcla de hidroxitirosol y 3,4-dihidroxifenilglicol, y extracto de acetato de hidroxitirosilo, a partir de subproductos del olivo y su purificación.
WO2016087428A1 (en) * 2014-12-04 2016-06-09 Dsm Ip Assets B.V. Fortification of edible oils with hyrdoxytyrosol
JP2017538432A (ja) 2014-12-17 2017-12-28 ユニバーシティ・カレッジ・ダブリン，ナショナル・ユニバーシティ・オブ・アイルランド，ダブリン フェノール基質の対応するカテコール生成物への酵素的変換のための方法
CN104628528B (zh) * 2015-01-05 2016-06-15 康美（北京）药物研究院有限公司 一种酪醇的制备方法
EP4301864A1 (de) * 2021-03-03 2024-01-10 Wacker Chemie AG Verfahren zur herstellung von hydroxytyrosol

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6746706B1 (en) * 1998-12-22 2004-06-08 Lipton, Division Of Conopco, Inc. Food compositions fortified with anti-oxidants

Also Published As

Publication number Publication date
EP1310562A1 (en) 2003-05-14
ES2170006A1 (es) 2002-07-16
US20030180833A1 (en) 2003-09-25
WO2002016628A1 (es) 2002-02-28
AU2001282147A1 (en) 2002-03-04

Similar Documents

Publication Publication Date Title
ES2170006B1 (es) Sintesis enzimatica del antioxidante hidroxitirosol.
Lane et al. The origin of membrane bioenergetics
BR112012024902A2 (pt) agente de cloro combinado e produção e uso do mesmo
ATE546190T1 (de) Vorrichtungen für die behandlung von aortenklappenstenose
EP1176210A4 (en) PREPARATION CONTAINING A CELL EXTRACT FOR PRODUCING A CELL-FREE PROTEIN AND DEVICE FOR PRODUCING A CELL-FREE PROTEIN
ATE401916T1 (de) Beutelanordnung mit mehreren fächern mit dialyseflüssigkeit
TW200709845A (en) Process for producing a porous track membrane
ATE294597T1 (de) Lösung für die peritonealdialyse
EP2338511A3 (en) Prolongation of survival of an allograft by inhibiting complement activity
Hoehler et al. Anaerobic methane oxidation by a methanogen-sulfate reducer consortium: geochemical evidence and biochemical considerations
CU23348A3 (es) Procedimiento para la filtración y estabilización de líquidos acuosos
ATE354626T1 (de) Vergasung von bioschlamm
CO5210885A1 (es) Barra de jabon novedosa y metodo para su preparacion
ATE459336T1 (de) Formulierung zur dermalen anwendung
RS51896B (en) The use of glucose isomerase in the treatment of fructose intolerance
PE20060543A1 (es) Proceso de bio-lixiviacion en estanques
PE20061000A1 (es) Dispositivo para desinfeccion y purificacion de agua y metodo
CN108341488A (zh) 一种用于治理黑臭水体的生物悬浮填料
Bishnoi Effects of thermal hydrolysis pre-treatment on anaerobic digestion of sludge
CN211367140U (zh) 一种基于mabr的新型氧化沟
ES2176166T3 (es) Procedimiento para la separacion enzimatica de la lactosa, especialmente mediante reactores de difusion por membrana.
Ragone Medical treatment of cystinuria with vitamin C
Glater Early history of reverse osmosis membrane development
ITMI20040070A1 (it) Modulo con supporto indeformabile per setti filtranti ed elementi a membrana
RU2008112524A (ru) Препарат для мезотерапии

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 2170006B1

Country of ref document: ES

GD2A Contractual licences

Effective date: 20070820

FD2A Announcement of lapse in spain

Effective date: 20180807