ES2167258A1 - Biosensor amperometrico composito para la determinacion de colesterol en alimentos. - Google Patents

Biosensor amperometrico composito para la determinacion de colesterol en alimentos.

Info

Publication number
ES2167258A1
ES2167258A1 ES200001918A ES200001918A ES2167258A1 ES 2167258 A1 ES2167258 A1 ES 2167258A1 ES 200001918 A ES200001918 A ES 200001918A ES 200001918 A ES200001918 A ES 200001918A ES 2167258 A1 ES2167258 A1 ES 2167258A1
Authority
ES
Spain
Prior art keywords
composite
cholesterol
foodstuffs
ethyl acetate
amperometric biosensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200001918A
Other languages
English (en)
Other versions
ES2167258B2 (es
Inventor
Carrazon Jose Manuel Pingarron
Garcia Angel Julio Reviejo
Garcia Nuria Pena
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Complutense de Madrid
Original Assignee
Universidad Complutense de Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Complutense de Madrid filed Critical Universidad Complutense de Madrid
Priority to ES200001918A priority Critical patent/ES2167258B2/es
Priority to PCT/ES2001/000304 priority patent/WO2002012550A1/es
Priority to AU2001282142A priority patent/AU2001282142A1/en
Publication of ES2167258A1 publication Critical patent/ES2167258A1/es
Application granted granted Critical
Publication of ES2167258B2 publication Critical patent/ES2167258B2/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

El Biosensor amperométrico compósito para la determinación de colesterol en alimentos consiste en una matriz electródica rígida y renovable por pulido fabricada con grafito y Teflón en polvo (70%) en la que se inmovilizan mediante simple inclusión física las enzimas colesterol oxidasa y peroxidasa junto con el mediador ferrocianuro potásico. El electrodo bienzimático compósito es compatible en medios predominantemente no acuosos como son las micelas inversas constituidas por un disolvente orgánico (acetato de etilo) como fase continua, una pequeña cantidad de una disolución acuosa de un electrólito apropiado (4% de una disolución reguladora de fosfato de pH 7,4), como fase dispersa y un tensoactivo (dioctilsulfosuccinato de sodio 0,1 M) como agente emulsificante. Esto permite la determinación directa del colesterol en estos medios una vez que se ha extraído del alimento utilizando como disolvente extractante el mismo disolvente con el que se forman las micelas inversas (acetato de etilo).
ES200001918A 2000-07-28 2000-07-28 Biosensor amperometrico composito para la determinacion de colesterol en alimentos. Expired - Fee Related ES2167258B2 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES200001918A ES2167258B2 (es) 2000-07-28 2000-07-28 Biosensor amperometrico composito para la determinacion de colesterol en alimentos.
PCT/ES2001/000304 WO2002012550A1 (es) 2000-07-28 2001-07-27 Biosensor amperometrico composito par la determinacion de colesterol en alimentos
AU2001282142A AU2001282142A1 (en) 2000-07-28 2001-07-27 Composite amperometric biosensor for determining cholesterol in foodstuffs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200001918A ES2167258B2 (es) 2000-07-28 2000-07-28 Biosensor amperometrico composito para la determinacion de colesterol en alimentos.

Publications (2)

Publication Number Publication Date
ES2167258A1 true ES2167258A1 (es) 2002-05-01
ES2167258B2 ES2167258B2 (es) 2003-03-01

Family

ID=8494495

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200001918A Expired - Fee Related ES2167258B2 (es) 2000-07-28 2000-07-28 Biosensor amperometrico composito para la determinacion de colesterol en alimentos.

Country Status (3)

Country Link
AU (1) AU2001282142A1 (es)
ES (1) ES2167258B2 (es)
WO (1) WO2002012550A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10211741B4 (de) * 2002-03-14 2005-12-01 November Ag Verfahren zum elektrochemischen Detektieren eines Analyten

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60247150A (ja) * 1984-05-23 1985-12-06 Sadaichi Murai ｐＨおよびイオン濃度測定に使用する比較電極用カ−ボン電極の製造方法
EP0251915A2 (en) * 1986-06-27 1988-01-07 Terumo Kabushiki Kaisha Enzyme sensor
EP0352925A2 (en) * 1988-07-28 1990-01-31 Cambridge Life Sciences Plc Enzyme electrodes and improvements in the manufacture thereof
EP0422623A2 (de) * 1989-10-13 1991-04-17 Gesellschaft für Biotechnologische Forschung mbH (GBF) Enzymelektrode und deren Verwendung
US5312590A (en) * 1989-04-24 1994-05-17 National University Of Singapore Amperometric sensor for single and multicomponent analysis
US5695947A (en) * 1995-06-06 1997-12-09 Biomedix, Inc. Amperometric cholesterol biosensor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60247150A (ja) * 1984-05-23 1985-12-06 Sadaichi Murai ｐＨおよびイオン濃度測定に使用する比較電極用カ−ボン電極の製造方法
EP0251915A2 (en) * 1986-06-27 1988-01-07 Terumo Kabushiki Kaisha Enzyme sensor
EP0352925A2 (en) * 1988-07-28 1990-01-31 Cambridge Life Sciences Plc Enzyme electrodes and improvements in the manufacture thereof
US5312590A (en) * 1989-04-24 1994-05-17 National University Of Singapore Amperometric sensor for single and multicomponent analysis
EP0422623A2 (de) * 1989-10-13 1991-04-17 Gesellschaft für Biotechnologische Forschung mbH (GBF) Enzymelektrode und deren Verwendung
US5695947A (en) * 1995-06-06 1997-12-09 Biomedix, Inc. Amperometric cholesterol biosensor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BASE DE DATOS WPI en QUESTEL, semana 198604, Londres: Derwent Publications Ltd., AN 1986-025019 & JP 60247150 A (MURA I.T.) *

Also Published As

Publication number Publication date
ES2167258B2 (es) 2003-03-01
WO2002012550A1 (es) 2002-02-14
AU2001282142A1 (en) 2002-02-18

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