ES2160062B1 - Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2. - Google Patents

Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.

Info

Publication number
ES2160062B1
ES2160062B1 ES009901557A ES9901557A ES2160062B1 ES 2160062 B1 ES2160062 B1 ES 2160062B1 ES 009901557 A ES009901557 A ES 009901557A ES 9901557 A ES9901557 A ES 9901557A ES 2160062 B1 ES2160062 B1 ES 2160062B1
Authority
ES
Spain
Prior art keywords
cox
promoter
cyclooxygenasa
witness
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES009901557A
Other languages
English (en)
Other versions
ES2160062A1 (es
Inventor
Escudero Manuel Fresno
Pena Miguel Angel Iniguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Esteve Pharmaceuticals SA
Original Assignee
Laboratorios del Dr Esteve SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES009901557A priority Critical patent/ES2160062B1/es
Application filed by Laboratorios del Dr Esteve SA filed Critical Laboratorios del Dr Esteve SA
Priority to AU58289/00A priority patent/AU778210B2/en
Priority to AT00944051T priority patent/ATE304060T1/de
Priority to JP2001509550A priority patent/JP2003504075A/ja
Priority to DK00944051T priority patent/DK1201766T3/da
Priority to PCT/ES2000/000245 priority patent/WO2001004350A1/es
Priority to CA002378921A priority patent/CA2378921A1/en
Priority to ES00944051T priority patent/ES2248088T3/es
Priority to DE60022519T priority patent/DE60022519T2/de
Priority to EP00944051A priority patent/EP1201766B1/en
Publication of ES2160062A1 publication Critical patent/ES2160062A1/es
Priority to ZA200200059A priority patent/ZA200200059B/en
Application granted granted Critical
Publication of ES2160062B1 publication Critical patent/ES2160062B1/es
Priority to US10/806,634 priority patent/US20040152126A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Magnetic Treatment Devices (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Línea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo, y su empleo en la búsqueda de inhibidores selectivos de la inducción transcripcional de cox- 2. La línea celular comprende una construcción de DNA que comprende la totalidad o parte de una secuencia promotora del gen de la ciclooxigenasa 2 (cox-2) y un gen testigo, unidos operativamente entre sí, de manera que dicha secuencia promotora del gen de la cox-2 dirige la expresión de dicho gen testigo en respuesta a un estímulo adecuado. El método de ensayo comprende poner en contacto dicha línea celular con el compuesto a ensayar y determinar la existencia de una señal indicativa de la expresión de actividad debida al gen testigo. Este método es adecuado para la búsqueda de inhibidores selectivos de la inducción a nivel transcripcional de la cox-2 por estímulos apropiados.
ES009901557A 1999-07-12 1999-07-12 Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2. Expired - Fee Related ES2160062B1 (es)

Priority Applications (12)

Application Number Priority Date Filing Date Title
ES009901557A ES2160062B1 (es) 1999-07-12 1999-07-12 Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.
DE60022519T DE60022519T2 (de) 1999-07-12 2000-07-11 Zellinie, enthaltend den promotor von cyclooxygenase-2 (cox-2) sowie ein zeugengen, und verwendung derselben in der suche von selektiven induktoren einer cox-2 transkriptionellen induktion
JP2001509550A JP2003504075A (ja) 1999-07-12 2000-07-11 シクロオキシゲナーゼ−２（ｃｏｘ−２）のプロモーターおよびリポーター遺伝子を含んでなる細胞系統、ならびにｃｏｘ−２の転写誘導の選択的阻害剤の探索におけるその使用
DK00944051T DK1201766T3 (da) 1999-07-12 2000-07-11 Cellelinie omfattende promotoren af cyclooxygenase-2 (cox-2) og et vidnegen samt anvendelse deraf til tilvejebringelse af selektive inhibitorer af cox-2-transskriptionsinduktion
PCT/ES2000/000245 WO2001004350A1 (es) 1999-07-12 2000-07-11 Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo, y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2
CA002378921A CA2378921A1 (en) 1999-07-12 2000-07-11 Cell line comprising the promoter of cyclooxygenase-2 (cox-2) and a witness gene, and use thereof in the search of cox-2 transcriptional induction selective inhibitors
AU58289/00A AU778210B2 (en) 1999-07-12 2000-07-11 Cell line comprising the promoter of cyclooxygenase-2 (COX-2) and a witness gene, and use thereof in the search of COX-2 transcriptional induction selective inhibitors
AT00944051T ATE304060T1 (de) 1999-07-12 2000-07-11 Zellinie, enthaltend den promotor von cyclooxygenase-2 (cox-2) sowie ein zeugengen, und verwendung derselben in der suche von selektiven induktoren einer cox-2 transkriptionellen induktion
EP00944051A EP1201766B1 (en) 1999-07-12 2000-07-11 Cell line comprising the promoter of cyclooxygenase-2 (cox-2) and a witness gene, and use thereof in the search of cox-2 transcriptional induction selective inhibitors
ES00944051T ES2248088T3 (es) 1999-07-12 2000-07-11 Linea celular que comprende el promotir de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.
ZA200200059A ZA200200059B (en) 1999-07-12 2002-01-03 Cell line comprising the promoter of cyclooxygenase-2-(cox-2) and a witness gene, and use thereof in the search of cox-2 transcriptional induction selective inhibitors.
US10/806,634 US20040152126A1 (en) 1999-07-12 2004-03-23 Methods of screening for cox-2 inhibitors

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009901557A ES2160062B1 (es) 1999-07-12 1999-07-12 Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.

Publications (2)

Publication Number Publication Date
ES2160062A1 ES2160062A1 (es) 2001-10-16
ES2160062B1 true ES2160062B1 (es) 2002-05-16

Family

ID=8309188

Family Applications (2)

Application Number Title Priority Date Filing Date
ES009901557A Expired - Fee Related ES2160062B1 (es) 1999-07-12 1999-07-12 Linea celular que comprende el promotor de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.
ES00944051T Expired - Lifetime ES2248088T3 (es) 1999-07-12 2000-07-11 Linea celular que comprende el promotir de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES00944051T Expired - Lifetime ES2248088T3 (es) 1999-07-12 2000-07-11 Linea celular que comprende el promotir de la ciclooxigenasa-2 (cox-2) y un gen testigo y su empleo en la busqueda de inhibidores selectivos de la induccion transcripcional de cox-2.

Country Status (10)

Country Link
EP (1) EP1201766B1 (es)
JP (1) JP2003504075A (es)
AT (1) ATE304060T1 (es)
AU (1) AU778210B2 (es)
CA (1) CA2378921A1 (es)
DE (1) DE60022519T2 (es)
DK (1) DK1201766T3 (es)
ES (2) ES2160062B1 (es)
WO (1) WO2001004350A1 (es)
ZA (1) ZA200200059B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
CA2466391A1 (en) * 2001-11-07 2003-05-15 Dennis P. Phillion Polyamide modulators of cox2 transcription
US7638331B2 (en) 2004-01-02 2009-12-29 The Administration of the Tulane Rducation Fund Directed apoptosis in COX-2 overexpressing cancer cells through expression targeted gene delivery
WO2006127731A2 (en) * 2005-05-23 2006-11-30 Ordway Research Institute, Inc. Inhibitors of pghs-2transactivator activity

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2164641A1 (en) * 1993-06-07 1994-12-22 Dinah S. Singer Use of an mhc class i suppressor drug for the treatment of autoimmune diseases and transplantation rejection
US5445941A (en) * 1993-06-21 1995-08-29 Eli Lilly And Company Method for screening anti-osteoporosis agents
US5569588A (en) * 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
WO1998037235A1 (en) * 1997-02-24 1998-08-27 Cornell Research Foundation, Inc. Method of screening agents as candidates for drugs or sources of drugs

Also Published As

Publication number Publication date
EP1201766B1 (en) 2005-09-07
ZA200200059B (en) 2003-04-03
DK1201766T3 (da) 2005-11-21
JP2003504075A (ja) 2003-02-04
DE60022519T2 (de) 2006-06-14
AU5828900A (en) 2001-01-30
AU778210B2 (en) 2004-11-25
DE60022519D1 (de) 2005-10-13
ATE304060T1 (de) 2005-09-15
WO2001004350A1 (es) 2001-01-18
ES2160062A1 (es) 2001-10-16
ES2248088T3 (es) 2006-03-16
CA2378921A1 (en) 2001-01-18
EP1201766A1 (en) 2002-05-02

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