ES2156127T3 - Linea celular de macrofagos para un ensayo de anticuerpos antinucleares; un metodo para detectar dichos anticuerpos empleando la linea celular de macrofagos y una preparacion para microscopio. - Google Patents

Linea celular de macrofagos para un ensayo de anticuerpos antinucleares; un metodo para detectar dichos anticuerpos empleando la linea celular de macrofagos y una preparacion para microscopio.

Info

Publication number
ES2156127T3
ES2156127T3 ES93916262T ES93916262T ES2156127T3 ES 2156127 T3 ES2156127 T3 ES 2156127T3 ES 93916262 T ES93916262 T ES 93916262T ES 93916262 T ES93916262 T ES 93916262T ES 2156127 T3 ES2156127 T3 ES 2156127T3
Authority
ES
Spain
Prior art keywords
cell line
cells
test
ana
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93916262T
Other languages
English (en)
Inventor
Think You Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1019920012928A external-priority patent/KR950011547B1/ko
Priority claimed from KR1019920012927A external-priority patent/KR960005733B1/ko
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2156127T3 publication Critical patent/ES2156127T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

ESTA INVENCION, RELACIONADA CON LA LINEA IT-1 (KCCN-10038) DE CELULAS MACROFAGAS, QUE SE DERIVA DE LA PARTE ESTRECHA DE HUESOS NORMALES, ES UN METODO PARA DETECTAR UN ANTICUERPO ANTINUCLEAR EMPLEANDO PARA ELLO LA CITADA LINEA DE CELULAS Y UNA PLETINA PARA LA PRUEBA ANA. EN LA PRUEBA ANA, LA LINEA DE CELULAS MACROFAGAS CONFORME A ESTA INVENCION MUESTRA UNA ADHERENCIA VITREA EXCELENTE SOBRE UNA PLETINA IF. Y POR ELLO, EL DEFECTO CONVENCIONAL DE LA LINEA DE CELULAS ESTANDO SUJETA A SER PELADA DESDE LA PLETINA SE PERFECCIONO, Y EL CONTENIDO DEL MEDIO DE CULTIVO EMPLEADO PUDO REDUCIRSE. TAMBIEN, LAS CELULAS IT-1 SE OBSERVO QUE FUERON POSITIVAS EN LA PRUEBA ANA, MIENTRAS QUE LAS CELULAS HEP-2 MOSTRARON RESULTADOS NEGATIVOS FALSOS, ESTO ES, LA POSIBILIDAD DE RESULTADOS NEGATIVOS FALSOS PUEDE SER SIGNIFICANTEMENTE DISMINUIDA CON CELULAS IT-1. Y DEBIDO A QUE EL TAMAÑO DE LAS CELULAS IT-1 ES MAYOR QUE EL DE LAS HEP-2, LOS RESULTADOS PUEDEN INTERPRETARSE FACILMENTE, EN ESPECIAL AL DETERMINAR SUBTIPOSDE ANTICUERPOS ANTINUCLEARES Y ANTICUERPOS ANTICITOPLASMICO. EL METODO PARA DETECTAR UN ANTICUERPO ANTINUCLEAR EMPLEANDO UNA LINEA DE CELULAS MACROFAGAS (KCCM-10038) CONFORME A ESTA INVENCION ES MUY UTIL AL DIAGNOSTICAR UNA ENFERMEDAD AUTO-INMUNE, TAL COMO ENFERMEDAD REUMATICA SISTEMATICA, ETC.
ES93916262T 1992-07-21 1993-07-21 Linea celular de macrofagos para un ensayo de anticuerpos antinucleares; un metodo para detectar dichos anticuerpos empleando la linea celular de macrofagos y una preparacion para microscopio. Expired - Lifetime ES2156127T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1019920012928A KR950011547B1 (ko) 1992-07-21 1992-07-21 항핵항체 진단용 슬라이드 및 그의 제작방법
KR1019920012927A KR960005733B1 (ko) 1992-07-21 1992-07-21 항핵항체 검출용 세포주 및 이를 이용한 항핵항체 검출방법

Publications (1)

Publication Number Publication Date
ES2156127T3 true ES2156127T3 (es) 2001-06-16

Family

ID=26629179

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93916262T Expired - Lifetime ES2156127T3 (es) 1992-07-21 1993-07-21 Linea celular de macrofagos para un ensayo de anticuerpos antinucleares; un metodo para detectar dichos anticuerpos empleando la linea celular de macrofagos y una preparacion para microscopio.

Country Status (7)

Country Link
US (1) US5583053A (es)
EP (1) EP0604635B1 (es)
JP (1) JP3721581B2 (es)
AU (1) AU4587193A (es)
DE (1) DE69330160T2 (es)
ES (1) ES2156127T3 (es)
WO (1) WO1994002594A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115409B1 (en) 1995-02-03 2006-10-03 Novozymes A/S α-amylase mutants
SE9900875D0 (sv) * 1999-03-11 1999-03-11 Eurodiagnostica Ab Antinuclear antibody assay utilizing stress-treated cells or cell extracts
AR064888A1 (es) * 2007-01-12 2009-04-29 Univ Illinois Celulas no simias para el crecimiento del virus del sindrome reproductor y respiratorio porcino (prrs)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL121073C (es) * 1962-01-30
US4487830A (en) * 1982-05-14 1984-12-11 American Hoechst Corporation Enzyme/immunofluorescent assay for autoantibodies
DE3683956D1 (de) * 1985-06-11 1992-04-02 Us Commerce In einem serumfreien medium in suspension kultivierte humane monozyten.
FR2657783B1 (fr) * 1990-02-07 1992-05-29 Fondation Nale Transfusion San Procede de preparation de derives sanguins et derives obtenus par ce procede.

Also Published As

Publication number Publication date
WO1994002594A1 (en) 1994-02-03
EP0604635B1 (en) 2001-04-18
EP0604635A1 (en) 1994-07-06
JPH06511390A (ja) 1994-12-22
US5583053A (en) 1996-12-10
AU4587193A (en) 1994-02-14
DE69330160D1 (de) 2001-05-23
DE69330160T2 (de) 2001-08-02
JP3721581B2 (ja) 2005-11-30

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