ES2155288B1 - CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES. - Google Patents

CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

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Publication number
ES2155288B1
ES2155288B1 ES9201750A ES9201750A ES2155288B1 ES 2155288 B1 ES2155288 B1 ES 2155288B1 ES 9201750 A ES9201750 A ES 9201750A ES 9201750 A ES9201750 A ES 9201750A ES 2155288 B1 ES2155288 B1 ES 2155288B1
Authority
ES
Spain
Prior art keywords
gene
diabetes
insulin
cdna
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9201750A
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Spanish (es)
Other versions
ES2155288A1 (en
Inventor
Tubert Fatima Bosch
Abril Alfons Valera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat Autonoma de Barcelona UAB
Original Assignee
Universitat Autonoma de Barcelona UAB
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Filing date
Publication date
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Priority to ES9201750A priority Critical patent/ES2155288B1/en
Publication of ES2155288A1 publication Critical patent/ES2155288A1/en
Application granted granted Critical
Publication of ES2155288B1 publication Critical patent/ES2155288B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Gen quimérico que utiliza el gen o CDNA de la insulina, en especial para terapia génica de la diabetes. El gen quimérico está dirigido por un promotor o fusión de promotores, los cuales preferentemente son regulables y están activados por el proceso diabético. Preferentemente, se obtiene por fusión del gen de la insulina humana al promotor de la PEPCK (p-enolpiruvato carboxiquinasa). Dicho promotor (fragmento - 460 pb a + 73 pb) está fusionado a la zona 5'' franqueante del gen de la insulina humana (-170 pb a +1). El gen de la insulina humana contiene dos exones codificantes E1 y E2 y dos intrones A y B. También se refiere a un vector de expresión que permite expresar insulina en células diferentes de las cálulas {be} del páncreas y a un animal transgénico no humano que expresa el citado gen quimérico. Se utiliza especialmente para la terapia génica de la diabetes.Chimeric gene that uses the insulin gene or CDNA, especially for diabetes gene therapy. The chimeric gene is directed by a promoter or fusion of promoters, which are preferably adjustable and activated by the diabetic process. Preferably, it is obtained by fusion of the human insulin gene to the PEPCK promoter (p-enolpyruvate carboxykinase). Said promoter (fragment - 460 bp at + 73 bp) is fused to the 5 '' free zone of the human insulin gene (-170 bp to +1). The human insulin gene contains two exons encoding E1 and E2 and two introns A and B. It also refers to an expression vector that allows insulin to be expressed in cells other than the callae {be} of the pancreas and a non-human transgenic animal that expresses the said chimeric gene. It is especially used for gene therapy of diabetes.

ES9201750A 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES. Expired - Fee Related ES2155288B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9201750A ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9201750A ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Publications (2)

Publication Number Publication Date
ES2155288A1 ES2155288A1 (en) 2001-05-01
ES2155288B1 true ES2155288B1 (en) 2001-12-01

Family

ID=8278035

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9201750A Expired - Fee Related ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Country Status (1)

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ES (1) ES2155288B1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010304A1 (en) * 1987-06-16 1988-12-29 Edison Animal Biotechnology Center Dietary and hormonal regulation of expression of exogenous genes in transgenic animals under control of the promoter of the gene for phosphoenolpyruvate carboxykinase

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Publication number Publication date
ES2155288A1 (en) 2001-05-01

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