ES2155288A1 - Gene for e.g. gene therapy of diabetes consists of the fusion product of the human insulin gene - Google Patents

Gene for e.g. gene therapy of diabetes consists of the fusion product of the human insulin gene

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Publication number
ES2155288A1
ES2155288A1 ES9201750A ES9201750A ES2155288A1 ES 2155288 A1 ES2155288 A1 ES 2155288A1 ES 9201750 A ES9201750 A ES 9201750A ES 9201750 A ES9201750 A ES 9201750A ES 2155288 A1 ES2155288 A1 ES 2155288A1
Authority
ES
Spain
Prior art keywords
gene
human insulin
promoter
diabetes
insulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9201750A
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Spanish (es)
Other versions
ES2155288B1 (en
Inventor
Tubert Fatima Bosch
Abril Alfons Valera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat Autonoma de Barcelona UAB
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Universitat Autonoma de Barcelona UAB
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Priority to ES9201750A priority Critical patent/ES2155288B1/en
Publication of ES2155288A1 publication Critical patent/ES2155288A1/en
Application granted granted Critical
Publication of ES2155288B1 publication Critical patent/ES2155288B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Chimeric gene using the insulin gene or cDNA, especially for gene therapy in diabetes.The chimeric gene is directed by a promoter or fusion of promoters, which are preferably regulated and activated by the diabetic process.Preferably it is obtained by fusion between the human insulin gene and the PEPCK promoter (P-enolpyruvate carboxykinase). Said promoter (fragment - 460 pb to + 73 pb) is fused with the 5' franking region of the human insulin gene (-170 pb to +1). The human insulin gene contains two exons encoding E1 and E2 and two introns, A and B. It also refers to a vector of expression which enables the expression of insulin in cells other than the [beta] cells of the pancreas and to a non-human transgenic animal which expresses the aforementioned chimeric gene. Notably for use in gene therapy for diabetes.< IMAGE >The gene for gene therapy of diabetes comprises the fusion product of the human insulin gene with PEPCK (p-enolpyruvate cosboxyquinase) promoter. A vector for expression of insulin in different cells from the pancreas is also disclosed.
ES9201750A 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES. Expired - Fee Related ES2155288B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9201750A ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9201750A ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Publications (2)

Publication Number Publication Date
ES2155288A1 true ES2155288A1 (en) 2001-05-01
ES2155288B1 ES2155288B1 (en) 2001-12-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES9201750A Expired - Fee Related ES2155288B1 (en) 1992-07-30 1992-07-30 CHEMICAL GENE THAT USES THE GENE OR CDNA OF INSULIN, ESPECIALLY FOR GENE THERAPY OF DIABETES.

Country Status (1)

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ES (1) ES2155288B1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010304A1 (en) * 1987-06-16 1988-12-29 Edison Animal Biotechnology Center Dietary and hormonal regulation of expression of exogenous genes in transgenic animals under control of the promoter of the gene for phosphoenolpyruvate carboxykinase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010304A1 (en) * 1987-06-16 1988-12-29 Edison Animal Biotechnology Center Dietary and hormonal regulation of expression of exogenous genes in transgenic animals under control of the promoter of the gene for phosphoenolpyruvate carboxykinase

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
EISENBERGER, C.L. et al. "Differential Regulation of the Rat Phosphoenolpyruvate Carboxykinase Gene Expression in Several Tissues of Transgenic Mice", MOLECULAR AND CELLULAR BIOLOGY, 1992, Vol. 12, No 3, paginas 1396-1403. Todo el documento. *
O'BRIEN, R.M. et al. "Identification of a Sequence in the PEPCK Gene that mediates a negative effect of Insulin on Transcription", SCIENCE, 1990, Vol. 249, paginas 533-537. Todo el documento. *
SHORT, M.K. et al. "Tissue-Specific, Developmental, Hormonal, and Dietary regulation of Rat Phosphoenolpyruvate Carboxykinase-Human Growth Hormone Fusion Genes in Transgenic Mice", MOLECULAR AND CELLULAR BIOLOGY, 1992, Vol. 12, No 3, paginas 1007-1020. Todo el documento. *
WYNSHAW-BORIS, A. et al. "Characterization of the Phosphoenolpyruvate Carboxykinase (GTP) Promoter-regulatory Region", THE JOURNAL OF BIOLOGICAL CHEMISTRY, 1986, Vol. 261, No 21, paginas 9714-9720. Todo el documento. *

Also Published As

Publication number Publication date
ES2155288B1 (en) 2001-12-01

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