ES2132084T3 - Matriz polimerica de elevada viscosidad y metodos de preparacion. - Google Patents

Matriz polimerica de elevada viscosidad y metodos de preparacion.

Info

Publication number
ES2132084T3
ES2132084T3 ES91903988T ES91903988T ES2132084T3 ES 2132084 T3 ES2132084 T3 ES 2132084T3 ES 91903988 T ES91903988 T ES 91903988T ES 91903988 T ES91903988 T ES 91903988T ES 2132084 T3 ES2132084 T3 ES 2132084T3
Authority
ES
Spain
Prior art keywords
matrix
high viscosity
preparation methods
polymeric matrix
components
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91903988T
Other languages
English (en)
Inventor
Robert S Dubrow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ES2132084T3 publication Critical patent/ES2132084T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Abstract

SE DESCRIBE UNA MATRIZ BASICAMENTE UNIFORME PARA USO EN SEPARACION INDUCIDA DE CAPO ELECTRICO DE COMPONENTES MOLECULARES EN UNA MUESTRA. LA MATRIZ SE PREPARA FORMANDO UNA MATRIZ POLIMERA VISCOELASTICA Y BOMBEANDO LA MATRIZ EN UN TUBO CAPILAR O TUBO A ESCALA PREPARATIVA. EL TIPO POLIMERO, LA CONCENTRACION, Y EL PESO MOLECULAR SE SELECCIONAN PARA OPTIMIZAR LA SEPARACION DE COMPONENTES DE ACIDOS NUCLEICOS O PROTEINAS. LA MATRIZ PUEDE EXPELERSE DEL TUBO, TRAS EL FRACCIONAMIENTO, PARA EL ANALISIS Y/O RECUPERACION EN COMPONENTES FRACCIONADOS.
ES91903988T 1990-01-29 1991-01-25 Matriz polimerica de elevada viscosidad y metodos de preparacion. Expired - Lifetime ES2132084T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/472,045 US5164055A (en) 1990-01-29 1990-01-29 High-viscosity polymer matrix and methods

Publications (1)

Publication Number Publication Date
ES2132084T3 true ES2132084T3 (es) 1999-08-16

Family

ID=23873989

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91903988T Expired - Lifetime ES2132084T3 (es) 1990-01-29 1991-01-25 Matriz polimerica de elevada viscosidad y metodos de preparacion.

Country Status (9)

Country Link
US (1) US5164055A (es)
EP (1) EP0513164B1 (es)
JP (1) JP2701091B2 (es)
AT (1) ATE178990T1 (es)
DE (1) DE69131124T2 (es)
DK (1) DK0513164T3 (es)
ES (1) ES2132084T3 (es)
GR (1) GR3030209T3 (es)
WO (1) WO1991011709A1 (es)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
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US5126021A (en) * 1991-07-17 1992-06-30 Applied Biosystems Inc. Low-viscosity polymer solution for capillary electrophoresis
WO1993015394A1 (en) * 1992-01-31 1993-08-05 Beckman Instruments, Inc. Capillary column containing removable separation gel composition and method of use
US5213669A (en) * 1992-01-31 1993-05-25 Beckman Instruments, Inc. Capillary column containing a dynamically cross-linked composition and method of use
US5290418A (en) * 1992-09-24 1994-03-01 Applied Biosystems, Inc. Viscous electrophoresis polymer medium and method
US5759369A (en) * 1992-09-24 1998-06-02 The Perkin-Elmer Corporation Viscous electrophoresis polymer medium and method
WO1994010561A1 (en) * 1992-11-05 1994-05-11 Soane Technologies, Inc. Separation media for electrophoresis
US5569364A (en) * 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
US5891313A (en) 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US6355709B1 (en) * 1993-12-17 2002-03-12 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
WO1995016910A1 (en) * 1993-12-17 1995-06-22 Perkin-Elmer Corporation Uncharged polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
JP3727031B2 (ja) * 1994-02-07 2005-12-14 アプレラ コーポレイション ポリヌクレオチド分析のための螢光に基づく電気泳動システム
US6485625B1 (en) 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US7244622B2 (en) * 1996-04-03 2007-07-17 Applera Corporation Device and method for multiple analyte detection
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5792331A (en) * 1996-12-19 1998-08-11 Dionex Corporation Preformed polymer coating process and product
WO1999017117A1 (en) * 1997-09-30 1999-04-08 Amira Medical Analytical device with capillary reagent carrier
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6117326A (en) * 1998-06-26 2000-09-12 Rohm And Haas Company Capillary electrochromatography separation media
JP2003523499A (ja) * 1999-01-12 2003-08-05 スペクトラメディックス コーポレイション キャピラリーゲル電気泳動のためのコポリマー
US6372106B1 (en) * 1999-07-26 2002-04-16 Applera Corporation Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
US6770698B1 (en) * 2000-06-05 2004-08-03 The Research Foundation At State University Of New York Polymer solution for separation of charged macromolecules by electrophoresis
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
AUPR161400A0 (en) * 2000-11-22 2000-12-14 Life Therapeutics Limited Apparatus and method for isoelectric focusing separations
US7347922B2 (en) * 2001-03-28 2008-03-25 Akins Jr Robert E Multi-dimensional proteomic analysis method
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
WO2003062810A1 (en) * 2002-01-22 2003-07-31 Board Of Regents, The University Of Texas System Electrophoretic ratchets and cyclic electrophoresis
JP2006505832A (ja) * 2002-02-26 2006-02-16 フアルマシア・コーポレーシヨン 配列決定システム計算機
US7001853B1 (en) 2002-08-30 2006-02-21 Caliper Life Sciences, Inc. Flow control of photo-polymerizable resin
US7083711B2 (en) * 2003-02-21 2006-08-01 Combisep, Inc. Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
US20040167480A1 (en) * 2003-02-21 2004-08-26 Advanced Medical Optics, Inc. Administration of multiple viscoelastic solutions with a multi-compartment syringe
US20050233390A1 (en) * 2003-04-09 2005-10-20 Allen John W Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
JP2009522554A (ja) 2005-12-29 2009-06-11 ライフ テクノロジーズ コーポレーション ポリアクリルアミドゲル上で分離される生体分子の分解能を改善するための組成物および方法
US8500980B1 (en) * 2006-10-24 2013-08-06 Qiagen Sciences, Llc Method and apparatus for high speed genotyping
JP5557733B2 (ja) 2007-04-04 2014-07-23 ネットバイオ・インコーポレーテッド プラスチック製マイクロ流体分離および検出プラットフォーム
EP2112196A1 (en) * 2008-04-25 2009-10-28 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO A liquid composition comprising polymer chains and particles of an inorganic material in a liquid
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
CA2825149C (en) 2011-01-31 2020-06-16 Primeradx, Inc. Reduced artifact denaturing capillary electrophoresis of nucleic acids
AU2013256229A1 (en) * 2012-05-03 2014-10-30 Medimmune, Llc Method for analyzing sample components
JP6992385B2 (ja) * 2017-10-02 2022-01-13 株式会社島津製作所 電気泳動用分離媒体、電気泳動用試薬キット、及び電気泳動方法

Also Published As

Publication number Publication date
DE69131124D1 (de) 1999-05-20
JPH06504612A (ja) 1994-05-26
EP0513164B1 (en) 1999-04-14
US5164055A (en) 1992-11-17
EP0513164A1 (en) 1992-11-19
JP2701091B2 (ja) 1998-01-21
GR3030209T3 (en) 1999-08-31
EP0513164A4 (es) 1994-04-20
ATE178990T1 (de) 1999-04-15
WO1991011709A1 (en) 1991-08-08
DE69131124T2 (de) 1999-09-23
DK0513164T3 (da) 1999-10-25

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