ES2130073B1 - Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal. - Google Patents
Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal.Info
- Publication number
- ES2130073B1 ES2130073B1 ES9701156A ES9701156A ES2130073B1 ES 2130073 B1 ES2130073 B1 ES 2130073B1 ES 9701156 A ES9701156 A ES 9701156A ES 9701156 A ES9701156 A ES 9701156A ES 2130073 B1 ES2130073 B1 ES 2130073B1
- Authority
- ES
- Spain
- Prior art keywords
- beta
- procedure
- xyloses
- usable
- diagnostic evaluation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Mejoras en el procedimiento de obtención de BETA-D-galactopiranosil-xilosas utilizables para la evaluación diagnostica de la lactasa intestinal. Procedimiento enzimático de obtención de BETA-D-galactopiranosil-xilosas utilizables para la evaluación diagnostica de la lactasa intestinal, caracterizado porque dicho procedimiento comprende: a) reacción de xilosa y un sustrato BETA-D-galactopiranósido en presencia de una enzima BETA-glicosidasa, siendo la concentración de xilosa de 2 a 20 veces superior a la del BETA-D-galactopiranósido, en un medio acuoso tamponado a pH comprendido entre 5,0 y 9,0 y a una temperatura comprendida entre 4 y 37ºC. b) desactivación de la enzima BETA-glicosidasa cuando se alcanza el máximo rendimiento de formación de disacáridos detectado mediante cromatografía en capa fina, c) aislamiento de los disacáridos formados mediante filtración en columnas de relleno, utilizándose como eluyente agua o mezclas agua/alcohol.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701156A ES2130073B1 (es) | 1997-05-28 | 1997-05-28 | Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal. |
AU72173/98A AU7217398A (en) | 1997-05-28 | 1998-05-14 | Process for obtaining beta-d-galactopyranosyl-xyloses usable for the diagnostic evaluation of intestinal lactase |
PCT/ES1998/000138 WO1998054356A1 (es) | 1997-05-28 | 1998-05-14 | PROCEDIMIENTO DE OBTENCION DE β-D-GALACTOPIRANOSIL-XILOSAS UTILIZABLES PARA LA EVALUACION DIAGNOSTICA DE LA LACTASA INTESTINAL |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701156A ES2130073B1 (es) | 1997-05-28 | 1997-05-28 | Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2130073A1 ES2130073A1 (es) | 1999-06-16 |
ES2130073B1 true ES2130073B1 (es) | 2000-04-01 |
Family
ID=8299475
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9701156A Expired - Fee Related ES2130073B1 (es) | 1997-05-28 | 1997-05-28 | Mejoras en el procedimiento de obtencion de beta-d-galactopiranosil-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal. |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU7217398A (es) |
ES (1) | ES2130073B1 (es) |
WO (1) | WO1998054356A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2182703B1 (es) * | 2001-06-18 | 2004-06-01 | Consejo Superior De Investigaciones Cientificas | Un procedimiento enzimatico para obtener 4-0-b-d-galactopiranosil-d-xilosa, 4-o-b-d-galactopiranosil-d-xilosa obtenida de acuerdo con el pr cedimiento, composiciones que la contienen y su uso en la evaluacion de la lactasa intestinal. |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2023556A6 (es) * | 1990-06-18 | 1992-01-16 | Consejo Superior Investigacion | Procedimiento de obtencion de 4-o-beta-d-galactopiranosil-d-xilosa utilizable para la evaluacion diagnostica de la lactasa intestinal. |
ES2100131B1 (es) * | 1995-11-08 | 1998-02-16 | Consejo Superior Investigacion | Procedimiento enzimatico de obtencion de beta-d-galactopiranosil-d-xilosas utilizables para la evaluacion diagnostica de la lactasa intestinal. |
-
1997
- 1997-05-28 ES ES9701156A patent/ES2130073B1/es not_active Expired - Fee Related
-
1998
- 1998-05-14 AU AU72173/98A patent/AU7217398A/en not_active Abandoned
- 1998-05-14 WO PCT/ES1998/000138 patent/WO1998054356A1/es active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO1998054356A1 (es) | 1998-12-03 |
AU7217398A (en) | 1998-12-30 |
ES2130073A1 (es) | 1999-06-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19990616 Kind code of ref document: A1 Effective date: 19990616 |
|
FD2A | Announcement of lapse in spain |
Effective date: 20190612 |