ES2115059T3 - Procedimiento para el diagnostico prenatal de anomalias geneticas. - Google Patents

Procedimiento para el diagnostico prenatal de anomalias geneticas.

Info

Publication number
ES2115059T3
ES2115059T3 ES93911748T ES93911748T ES2115059T3 ES 2115059 T3 ES2115059 T3 ES 2115059T3 ES 93911748 T ES93911748 T ES 93911748T ES 93911748 T ES93911748 T ES 93911748T ES 2115059 T3 ES2115059 T3 ES 2115059T3
Authority
ES
Spain
Prior art keywords
nucleated erythrocytes
pregnant women
various
blood
hybridisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93911748T
Other languages
English (en)
Inventor
Dorothee Ahlert
Wolfgang Holzgreve
Hendrikus Stephanus Garritsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2115059T3 publication Critical patent/ES2115059T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

TANTO EN MUESTRAS DE SANGRE DEL CORDON UMBILICAL COMO TAMBIEN DESPUES DE LA SEPARACION DE LA SANGRE DE LA EMBARAZADA HA SIDO POSIBLE DEMOSTRAR DE FORMA CLARA QUE LA COMBINACION DEL GRADIENTE TRIPLE Y MACS CON CELULAS MARCADAS ANTI-CD71 ES UN METODO EFECTIVO DE ENRIQUECIMIENTO DE ERITROCITOS NUCLEADOS. LA PROPORCION DE ERITROCITOS NUCLEADOS EN LA SANGRE DEL CORDON UMBILICAL ESTA ENTRE 72 Y 89% DESPUES DE AMBOS METODOS DE ENRIQUECIMIENTO. HA SIDO POSIBLE LA DETECCION DE ERITROCITOS NUCLEADOS EN LA FRACCION POSITIVA DE ACUERDO CON EL GRADIENTE TRIPLE Y MACS EN TODOS LOS CASOS EN MUJERES EMBARAZADAS CON DIFERENTES ETAPAS DE GESTACION. LA VARIACION EN EL NUMERO DE ERITROCITOS NUCLEADOS ENRIQUECIDOS EN VARIOS CASOS DE EMBARAZO REFLEJA MUY PROBABLEMENTE DIFERENCIAS INDIVIDUALES EN LA RELACION FETO-CELULA MATERNAL EN VARIAS ETAPAS DE EMBARAZO. EL PROCESO AQUI DESCRITO HACE POSIBLE EN SANGRE DE VARON NORMAL Y EN MUJERES NO EMBARAZADAS DEMOSTRAR LA NO PRESENCIA DE ERITROCITOS NUCLEADOS, EN CONTRASTECON LAS MUJERES EMBARAZADAS EN VARIAS ETAPAS DE GESTACION. EL METODO ES TAMBIEN ALTAMENTE REPRODUCIBLE Y ADECUADO PARA DIAGNOSIS CLINICA. MEDIANTE FLUORESCENCIA DE HIBRIDIZACION IN SITU HA SIDO POSIBLE DETECTAR UNA TRISOMIA FETAL EN LOS TRES CASOS INVESTIGADOS. EL ENRIQUECIMIENTO DE ERITROCITOS NUCLEADOS ES POR TANTO SUFICIENTEMENTE FUERTE PARA DIAGNOSTICAR ANEUPLOIDES INFANTILES UTILIZANDO FLUORESCENCIA DE HIBRIDIZACION IN SITU. ES POR TANTO OBVIO QUE ES POSIBLE CON ESTO SOLAMENTE DE FORMA DE INVASION LIGERA Y RELATIVAMENTE SIMPLE ASI COMO UN METODO ECONOMICO PARA LA REALIZACION DE EXAMINACIONES DE EXPLORACION DE TRES DE LAS MAS IMPORTANTES TRISOMIAS (13,18 Y 21) Y ENFERMEDADES MONOGENETICAS (CON LA AYUDA DEL PCR) SIN QUE EXISTA VIRTUALMENTE RIESGO PARA LA PACIENTE Y PARA EL NIÑO.
ES93911748T 1992-05-16 1993-05-11 Procedimiento para el diagnostico prenatal de anomalias geneticas. Expired - Lifetime ES2115059T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4216345 1992-05-16
DE4222573A DE4222573C1 (es) 1992-05-16 1992-07-09

Publications (1)

Publication Number Publication Date
ES2115059T3 true ES2115059T3 (es) 1998-06-16

Family

ID=25914908

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93911748T Expired - Lifetime ES2115059T3 (es) 1992-05-16 1993-05-11 Procedimiento para el diagnostico prenatal de anomalias geneticas.

Country Status (9)

Country Link
EP (1) EP0597068B1 (es)
JP (1) JPH06509178A (es)
AT (1) ATE163093T1 (es)
AU (1) AU667723B2 (es)
CA (1) CA2113194A1 (es)
DE (2) DE4222573C1 (es)
DK (1) DK0597068T3 (es)
ES (1) ES2115059T3 (es)
WO (1) WO1993023754A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489386A (en) * 1994-01-31 1996-02-06 Applied Imaging Density gradient medium for the separation of cells
US5432054A (en) * 1994-01-31 1995-07-11 Applied Imaging Method for separating rare cells from a population of cells
GB9603249D0 (en) * 1996-02-16 1996-04-17 Univ Nottingham Foetal cell analysis
CA2283416A1 (en) * 1997-03-08 1998-09-17 The University Of Dundee Prenatal diagnostic methods
AU5967100A (en) * 1999-07-21 2001-02-13 Britta Christensen A novel method for the improved isolation of a target cell population
JP6234542B1 (ja) 2016-12-27 2017-11-22 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来染色体ｄｎａの取得方法
SG11202002907RA (en) * 2017-10-19 2020-05-28 Tl Genomics Inc Cell classification chip
JP2020103299A (ja) * 2020-02-18 2020-07-09 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来の核酸の取得方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4434156A (en) * 1981-10-26 1984-02-28 The Salk Institute For Biological Studies Monoclonal antibodies specific for the human transferrin receptor glycoprotein
AU621694B2 (en) * 1988-12-06 1992-03-19 Flinders Technologies Pty Ltd Isolation of fetal cells from maternal blood to enable prenatal diagnosis
ATE161960T1 (de) * 1988-12-06 1998-01-15 Flinders Technologies Pty Ltd Isolierung von fetalen zellen aus mütterlichem blut zur ausführung von pränataler diagnostik
FR2657167B1 (fr) * 1990-01-16 1992-05-07 Clonatec Sa Procede de depistage prenatal in vitro de la trisomie 21 fóoetale dans un echantillon biologique susceptible de contenir de l'hcg et necessaire pour sa mise en óoeuvre.

Also Published As

Publication number Publication date
DE4222573C1 (es) 1993-08-12
EP0597068A1 (de) 1994-05-18
DK0597068T3 (da) 1998-09-23
JPH06509178A (ja) 1994-10-13
CA2113194A1 (en) 1993-11-25
AU667723B2 (en) 1996-04-04
DE59308101D1 (de) 1998-03-12
EP0597068B1 (de) 1998-02-04
WO1993023754A1 (de) 1993-11-25
ATE163093T1 (de) 1998-02-15
AU4259793A (en) 1993-12-13

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