ES2113873T3 - Aparato de electroforesis en gel horizontal. - Google Patents
Aparato de electroforesis en gel horizontal.Info
- Publication number
- ES2113873T3 ES2113873T3 ES91307761T ES91307761T ES2113873T3 ES 2113873 T3 ES2113873 T3 ES 2113873T3 ES 91307761 T ES91307761 T ES 91307761T ES 91307761 T ES91307761 T ES 91307761T ES 2113873 T3 ES2113873 T3 ES 2113873T3
- Authority
- ES
- Spain
- Prior art keywords
- gel
- gel electrophoresis
- electrophoresis apparatus
- site
- sample introduction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
- Colloid Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Devices For Indicating Variable Information By Combining Individual Elements (AREA)
- Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
SE DESCRIBE UN CONJUNTO DE ELECTROFORESIS HORIZONTAL DISEÑADO PARA UTILIZAR GELES MUY FINOS DE POLIACRILAMIDA. EL CONJUNTO PERMITE LA REFRIGERACION POR AGUA DEL GEL DURANTE LA ELECTROFORESIS PARA QUE SE PUEDAN APLICAR ALTOS VOLTAJES AL GEL SIN DEGRADACION DE POLIMEROS. EL MOLDE DE GEL SE ELABORA A PARTIR DE UN JUEGO DE PLACAS DE VIDRIO SUJETADOS EN SU LUGAR DEFINIENDO UN MOLDE DE GEL ENTRE ELLAS. EN UNA EJEMPLIFICACION, SE SEPARA EL SITIO DE INTRODUCCION DE CAMPO DEL SITIO DE INTRODUCCION DE MUESTRA DE MANERA QUE EL CAMPO ELECTRICO SEA GENERALMENTE LINEAL EN LA ZONA DE INTRODUCCION DE LA MUESTRA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/630,675 US5137613A (en) | 1990-12-20 | 1990-12-20 | Horizontal gel electrophoresis apparatus |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2113873T3 true ES2113873T3 (es) | 1998-05-16 |
Family
ID=24528123
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91307761T Expired - Lifetime ES2113873T3 (es) | 1990-12-20 | 1991-08-22 | Aparato de electroforesis en gel horizontal. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5137613A (es) |
EP (1) | EP0492769B1 (es) |
JP (1) | JPH04335152A (es) |
AT (1) | ATE161632T1 (es) |
CA (1) | CA2049749A1 (es) |
DE (1) | DE69128522T2 (es) |
ES (1) | ES2113873T3 (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5242568A (en) * | 1992-01-14 | 1993-09-07 | Fotodyne Incorporated | Electrophoresis apparatus |
US5338426A (en) * | 1992-08-28 | 1994-08-16 | Applied Biosystems, Inc. | Horizontal polyacrylamide gel electrophoresis |
US5449446A (en) * | 1994-03-09 | 1995-09-12 | Verma; Sumeet | Multi-purpose electrophoresis apparatus |
US5507934A (en) * | 1994-11-01 | 1996-04-16 | Visible Genetics Inc. | Apparatus for preparing gels for use in electrophoretic separations and similar applications |
US6485625B1 (en) | 1995-05-09 | 2002-11-26 | Curagen Corporation | Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments |
US6017434A (en) * | 1995-05-09 | 2000-01-25 | Curagen Corporation | Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments |
US5549806A (en) * | 1996-02-21 | 1996-08-27 | Chen; Stephen L. | Device and method of direct water cooling for horizontal submarine gel electrophoresis |
US5759375A (en) * | 1996-05-17 | 1998-06-02 | Purdue Research Foundation | Miniaturized disposable gels for DNA analysis |
US6207354B1 (en) | 1999-04-07 | 2001-03-27 | International Business Machines Coporation | Method of making an organic chip carrier package |
US20010037940A1 (en) * | 2000-01-28 | 2001-11-08 | Lih-Bin Shih | Apparatus for horizontal gel electrophoresis and method of using same |
JP2002048765A (ja) * | 2000-08-03 | 2002-02-15 | Masabumi Youda | 水平面型ゲル電気泳動装置用試料導入装置、水平面型ゲル電気泳動システム、その試料導入方法およびその試料採取方法 |
CA2434469A1 (en) * | 2001-01-12 | 2002-07-18 | Syngenta Participations Ag | Thin film electrophoresis apparatus and method |
US20020127148A1 (en) * | 2001-03-09 | 2002-09-12 | Sanjay Kumar | Gel processing and transfer device |
AUPR800701A0 (en) * | 2001-09-28 | 2001-10-25 | Proteome Systems Ltd | Cassette for electrophoresis |
DE50302642D1 (de) * | 2002-04-12 | 2006-05-11 | Tecan Trading Ag | Kassette, System und 2D-Gelelektrophorese-Verfahren zum Trennen von Molekülen |
US7303715B1 (en) | 2002-12-04 | 2007-12-04 | Owl Separation Systems, Inc. | Horizontal gel casting device and method |
US20040171167A1 (en) * | 2002-12-13 | 2004-09-02 | Affymetrix, Inc. | Chip-in-a-well scanning |
US20080164149A1 (en) * | 2007-01-05 | 2008-07-10 | Artz Matthew R | Rapid gel electrophoresis system |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3888759A (en) * | 1973-05-25 | 1975-06-10 | Yeda Res & Dev | Flat plate electrophoresis |
JPS5416281A (en) * | 1978-04-07 | 1979-02-06 | Fuji Glass | Method of melting and closing branch tube of ampule |
DE3024288C2 (de) * | 1980-06-27 | 1982-03-25 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Verfahren und Vorrichtung zur Herstellung dünner Schichten |
DE3046729C2 (de) * | 1980-12-11 | 1985-05-15 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Thermostatische Platte für eine Elektrophoreseeinrichtung |
FR2496889A1 (fr) * | 1980-12-22 | 1982-06-25 | Hours Michel | Cuve de migration et de reaction utilisable pour des analyses par electrophorese |
US4588491A (en) * | 1984-02-27 | 1986-05-13 | International Biotechnologies, Inc. | Horizontal gel electrophoresis device |
US4693804A (en) * | 1984-12-19 | 1987-09-15 | Board Of Regents, The University Of Texas System | Apparatus for bidimensional electrophoretic separations |
US4612106A (en) * | 1984-12-24 | 1986-09-16 | Kromer Heiner M | Cooling system for a slab gel electrophoresis apparatus |
US4608146A (en) * | 1985-04-09 | 1986-08-26 | Bio-Rad Laboratories, Inc. | Horizontal electrophoresis cell for rapid assembly |
US4756809A (en) * | 1986-02-04 | 1988-07-12 | Oncor, Inc. | Process for conducting electrophoresis and transfer |
US4849078A (en) * | 1986-09-25 | 1989-07-18 | Oncor, Inc. | Process for conducting electrophoresis and transfer |
US4773984A (en) * | 1987-02-02 | 1988-09-27 | Life Technologies, Inc. | Vertical gel slab electrophoresis apparatus |
US4909977A (en) * | 1987-05-20 | 1990-03-20 | Bios Corporation | Method for molding thin gel slabs horizontally with integrally molded large volume sample wells |
US4795541A (en) * | 1987-05-20 | 1989-01-03 | Bios Corporation | Method and apparatus for molding thin gel slabs horizontally with integrally molded large volume sample wells |
JPS63317756A (ja) * | 1987-06-19 | 1988-12-26 | Fuji Photo Film Co Ltd | 電気泳動装置 |
US4865714A (en) * | 1987-07-17 | 1989-09-12 | Beckman Instruments, Inc. | Electrophoretic gel cooled cell |
US4913791A (en) * | 1987-08-28 | 1990-04-03 | Bios Corporation | Blot frame and method of handling |
US4830726A (en) * | 1988-02-03 | 1989-05-16 | The Wistar Institute | Separation of large DNA molecules in alternating asymmetric electric fields |
US4919784A (en) * | 1989-08-16 | 1990-04-24 | Eg&G, Inc. | Electrophorresis cassette spacer |
-
1990
- 1990-12-20 US US07/630,675 patent/US5137613A/en not_active Expired - Fee Related
-
1991
- 1991-08-22 AT AT91307761T patent/ATE161632T1/de not_active IP Right Cessation
- 1991-08-22 DE DE69128522T patent/DE69128522T2/de not_active Expired - Fee Related
- 1991-08-22 ES ES91307761T patent/ES2113873T3/es not_active Expired - Lifetime
- 1991-08-22 EP EP91307761A patent/EP0492769B1/en not_active Expired - Lifetime
- 1991-08-23 CA CA002049749A patent/CA2049749A1/en not_active Abandoned
- 1991-10-16 JP JP3267455A patent/JPH04335152A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
DE69128522T2 (de) | 1998-08-13 |
US5137613A (en) | 1992-08-11 |
CA2049749A1 (en) | 1992-06-21 |
EP0492769B1 (en) | 1997-12-29 |
ATE161632T1 (de) | 1998-01-15 |
JPH04335152A (ja) | 1992-11-24 |
DE69128522D1 (de) | 1998-02-05 |
EP0492769A2 (en) | 1992-07-01 |
EP0492769A3 (en) | 1993-11-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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