ES2094088A1 - Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina. - Google Patents

Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina.

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Publication number
ES2094088A1
ES2094088A1 ES09400931A ES9400931A ES2094088A1 ES 2094088 A1 ES2094088 A1 ES 2094088A1 ES 09400931 A ES09400931 A ES 09400931A ES 9400931 A ES9400931 A ES 9400931A ES 2094088 A1 ES2094088 A1 ES 2094088A1
Authority
ES
Spain
Prior art keywords
penicillin
chrysogenum
region
superproducers
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09400931A
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English (en)
Other versions
ES2094088B1 (es
Inventor
Fuente Jose Luis Barredo
Garcia Bruno Diez
De La Vieja Alfonso Collados
Alba Alejandro Vitaller
Maldonado Francisco Salto
Fierro Francisco Fierro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES9400931A priority Critical patent/ES2094088B1/es
Publication of ES2094088A1 publication Critical patent/ES2094088A1/es
Application granted granted Critical
Publication of ES2094088B1 publication Critical patent/ES2094088B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

IDENTIFICACION, CLONACION Y UTILIZACION DE UNA REGION DE DNA AMPLIFICADA EN CEPAS DE CVAP. CHRYSOGENUM SUPERPRODUCTORAS DE PENICILINA. DICHA REGION AMPLIFICADA POSEE UN TAMAÑO DE 57 KB EN CVAP. CHRYSOGENUM E-1 Y DE106 KB EN CVAP. CHRYSOGENUM AS-P-78 E INCLUYE LOS GENES BIOSINTETICOS DE PENICILINA PCBAB (DE-(L-AL-AMINOADIPIL)-L-CISTEINIL-D-VALINA SINTETASA), PCBC (ISOPENICILINA-N SINTASA) Y PENDE (ACIL-COA:6-APA ACILTRANSFERASA), LOS CUALES SE EXTIENDEN A LO LARGO DE 16,5 KB. TAMBIEN SE ESTABLECE UNA RELACION DIRECTA ENTRE NUMERO DE COPIAS DE DICHA REGION Y LA CAPACIDAD DE PRODUCCION DE PENICILINA. ASIMISMO SE PRESENTAN UNA SERIE DE APLICACIONES TENDENTES A INCREMENTAR EL NUMERO DE COPIAS DE DICHA REGION EN UNA DETERMINADA CEPA Y A MEJORAR POR CONSIGUIENTE SU PRODUCCION DE PENICILINA.
ES9400931A 1994-05-04 1994-05-04 Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina. Expired - Lifetime ES2094088B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9400931A ES2094088B1 (es) 1994-05-04 1994-05-04 Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9400931A ES2094088B1 (es) 1994-05-04 1994-05-04 Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina.

Publications (2)

Publication Number Publication Date
ES2094088A1 true ES2094088A1 (es) 1997-01-01
ES2094088B1 ES2094088B1 (es) 1997-09-01

Family

ID=8286088

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9400931A Expired - Lifetime ES2094088B1 (es) 1994-05-04 1994-05-04 Identificacion, clonacion y utilizacion de una region de dna amplificada en cepas de p. chrysogenum superproductoras de penicilina.

Country Status (1)

Country Link
ES (1) ES2094088B1 (es)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998046772A2 (en) 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
WO1999031244A1 (de) * 1997-12-12 1999-06-24 Biochemie Gesellschaft M.B.H. NEUER TRANSKRIPTIONSFAKTOR CPCR1 ZUR REGULATION DES ISOPENICILLIN N-SYNTHETASE-GENS (PCbC) UND DES AC-/ACV-SYNTHETASE-GENS (pcbAB)
WO2001042426A1 (es) * 1999-12-10 2001-06-14 Consejo Superior De Investigaciones Científicas Produccion de penicilina mediante la utilización de cepas transgenicas merodiploides

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARREDO, J.L. et al., Large amplification of a 35-kb DNA fragment carrying two penicillin biosynthetic genes in high penicillin producing strains of Penicillium chrysogenum, Curr. Genet., 1989, Vol. 16, pßgs. 453-459. *Ver todo el documento* *
FIERRO, F. et al., Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.8 Mb) in Penicillium notatum and chromosome I (10.4 Mb) in Penicillium chrysogenum, Mol. Gen. Genet., 1993, Vol. 241, pßgs. 573-578. *Ver todo el documento* *
KœNKEL, W. et al., Genetic instability of industrial strains of Penicillium chrysogenum, Appl. Microbiol. Biotechnol., 1992, Vol. 36, pßgs. 499-502. *Ver todo el documento* *
SMITH, D.J. et al., Amplification of the isopenicillin N synthetase gene in a strain of Penicillium chrysogenum producing high levels of penicillin, Mol. Gen. Genet., 1989, Vol. 216, pßgs. 492-497. *Ver todo el documento* *
WALZ, M. et al., Polymorphic karyotypes in related Acremonium strains, Curr. Genet., 1991, Vol. 19, pßgs. 73-76. *Ver todo el documento* *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998046772A2 (en) 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
EP2298913A1 (en) 1997-04-11 2011-03-23 DSM IP Assets B.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
WO1999031244A1 (de) * 1997-12-12 1999-06-24 Biochemie Gesellschaft M.B.H. NEUER TRANSKRIPTIONSFAKTOR CPCR1 ZUR REGULATION DES ISOPENICILLIN N-SYNTHETASE-GENS (PCbC) UND DES AC-/ACV-SYNTHETASE-GENS (pcbAB)
WO2001042426A1 (es) * 1999-12-10 2001-06-14 Consejo Superior De Investigaciones Científicas Produccion de penicilina mediante la utilización de cepas transgenicas merodiploides
ES2164574A1 (es) * 1999-12-10 2002-02-16 Consejo Superior Investigacion Mejora de la produccion de penicilina mediante la utilizacion de cepas transgenicas merodiploides para un gen regulador.

Also Published As

Publication number Publication date
ES2094088B1 (es) 1997-09-01

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