ES2084839T3 - Metodo y medios para sintesis de oligonucleotidos. - Google Patents

Metodo y medios para sintesis de oligonucleotidos.

Info

Publication number
ES2084839T3
ES2084839T3 ES91920800T ES91920800T ES2084839T3 ES 2084839 T3 ES2084839 T3 ES 2084839T3 ES 91920800 T ES91920800 T ES 91920800T ES 91920800 T ES91920800 T ES 91920800T ES 2084839 T3 ES2084839 T3 ES 2084839T3
Authority
ES
Spain
Prior art keywords
pct
oligonucleotides
synthesis
aralkyloxy
cycloalkyloxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91920800T
Other languages
English (en)
Inventor
Lars Holmberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech AB filed Critical Pharmacia Biotech AB
Application granted granted Critical
Publication of ES2084839T3 publication Critical patent/ES2084839T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)

Abstract

SISTEMA DE SOPORTE PARA LA SINTESIS DE OLIGONUCLEOTIDOS EN EL QUE EL PRIMER NUCLEOSIDO ESTA ENLAZADO AL SOPORTE VIA UN ENLACE SILILETER, ESPECIALMENTE CON LA FORMULA (I), EN DONDE B ES UN NUCLEOSIDO O BASE DEOXINUCLEOSIDA. R1 ES UN GRUPO PROTECTOR. R2 ES -H, -OH O -OR5, EN DONDE R5 ES UN GRUPO PROTECTOR. R3, R4 Y X, CADA UNO, REPRESENTA ALQUILO, ARILO, CICLOALQUILO, ALQUENILO, ARALQUILO, CICLOALQUILALQUILO, ALQUILOXI, ARILOXI, CICLOALQUILOXI, ARALQUILOXI. S ES UN SOPORTE SOLIDO, DE ESTE MODO, NO MAS DE UN R3, R4 Y X REPRESENTA ALQUILOXI, ARILOXI, CICLOALQUILOXI, ALQUENILOXI, ARALQUILOXI O CICLOALQUILALQUILOXI.
ES91920800T 1990-11-26 1991-11-25 Metodo y medios para sintesis de oligonucleotidos. Expired - Lifetime ES2084839T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9003743A SE9003743D0 (sv) 1990-11-26 1990-11-26 Method and means for oligonucleotide synthesis

Publications (1)

Publication Number Publication Date
ES2084839T3 true ES2084839T3 (es) 1996-05-16

Family

ID=20380998

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91920800T Expired - Lifetime ES2084839T3 (es) 1990-11-26 1991-11-25 Metodo y medios para sintesis de oligonucleotidos.

Country Status (8)

Country Link
US (1) US5589586A (es)
EP (1) EP0514513B1 (es)
JP (1) JP3161730B2 (es)
AT (1) ATE133684T1 (es)
DE (1) DE69116864T2 (es)
ES (1) ES2084839T3 (es)
SE (1) SE9003743D0 (es)
WO (1) WO1992009615A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5342631A (en) * 1992-12-29 1994-08-30 Wm. Wrigley Jr. Company Wax-free chewing gum including special oligosaccharide binders
JPH06169754A (ja) * 1992-12-04 1994-06-21 Hideo Hirano 合成オリゴヌクレオタイドの精製装置
GB9307014D0 (en) * 1993-04-02 1993-05-26 Laporte Plc Protecting group for use in oligodeoxyribonucleotide synthesis
SE9603171D0 (sv) 1996-08-30 1996-08-30 Marek Kwiatkowski Solid phase synthesis
ES2114504B1 (es) * 1996-09-13 1999-02-01 Univ Madrid Autonoma Metodo para la sintesis enzimatica de desoxioligonucleotidos en soporte solido.
SE9701783D0 (sv) * 1997-05-14 1997-05-14 Marek Kwiatkowski In situ synthesis of oligonucleotides of inverse orientation
GB9815166D0 (en) * 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds for mass spectrometry
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
DE60042962D1 (de) * 1999-02-05 2009-10-29 Ge Healthcare Bio Sciences Verfahren zum entschützen von oligonukleotiden
GB9907245D0 (en) * 1999-03-29 1999-05-26 Goldsborough Andrew Cleavage of nucleic acids from solid supports
SE9904506D0 (sv) * 1999-12-09 1999-12-09 Karolinska Innovations Ab Method for immobilisation
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
CA2714353A1 (en) 2000-12-05 2002-06-13 Avecia Biotechnology Inc Process for the preparation of phosphorothioate oligonucleotides
US20040185459A1 (en) * 2001-04-18 2004-09-23 Masami Otsuka Hybridization probe
WO2002088160A1 (en) * 2001-04-30 2002-11-07 Avecia Biotechnology Inc. Immobilization of oligonucleotides onto solid supports
US6818762B2 (en) 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
WO2005040357A2 (en) * 2003-10-24 2005-05-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US7612759B2 (en) * 2004-05-12 2009-11-03 Shimano Inc. Cycle computer display apparatus
US7671218B2 (en) 2004-08-13 2010-03-02 Elitech Holdings B.V. Phosphonate fluorescent dyes and conjugates
US8129517B1 (en) 2006-05-23 2012-03-06 Integrated Dna Technologies, Inc. Labeled solid supports for organic synthesis
US8677232B2 (en) 2011-05-31 2014-03-18 Apple Inc. Devices, methods, and graphical user interfaces for document manipulation
CA3011583C (en) 2017-07-26 2021-01-26 Smith Industries, Inc. D/B/A Jay R. Smith Mfg. Co. Inlay tray threshold trench drain

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX158743A (es) * 1980-02-29 1989-03-10 University Patents Inc Procedimiento para la produccion de oligonucleotidos
FR2529892A1 (fr) * 1982-07-09 1984-01-13 Transgene Sa Nouveau support pour la synthese des polynucleotides, en particulier par la methode triester et procede pour sa preparation
FR2584090B1 (fr) * 1985-06-27 1987-08-28 Roussel Uclaf Nouveaux supports, leur preparation et les intermediaires obtenus, leur application a la synthese d'oligonucleotides et les nouveaux nucleosides et oligonucleotides relies aux supports ainsi obtenus

Also Published As

Publication number Publication date
ATE133684T1 (de) 1996-02-15
WO1992009615A1 (en) 1992-06-11
SE9003743D0 (sv) 1990-11-26
JP3161730B2 (ja) 2001-04-25
DE69116864D1 (de) 1996-03-14
EP0514513B1 (en) 1996-01-31
DE69116864T2 (de) 1996-09-12
EP0514513A1 (en) 1992-11-25
US5589586A (en) 1996-12-31
JPH05503715A (ja) 1993-06-17

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