ES2079319A1 - Bacteriological culture medium and process for its preparation - Google Patents

Bacteriological culture medium and process for its preparation

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Publication number
ES2079319A1
ES2079319A1 ES09401056A ES9401056A ES2079319A1 ES 2079319 A1 ES2079319 A1 ES 2079319A1 ES 09401056 A ES09401056 A ES 09401056A ES 9401056 A ES9401056 A ES 9401056A ES 2079319 A1 ES2079319 A1 ES 2079319A1
Authority
ES
Spain
Prior art keywords
preparation
agar
deionized water
culture medium
novobiocin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09401056A
Other languages
Spanish (es)
Other versions
ES2079319B1 (en
Inventor
Aguayo Jose Maria Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to ES9401056A priority Critical patent/ES2079319B1/en
Publication of ES2079319A1 publication Critical patent/ES2079319A1/en
Application granted granted Critical
Publication of ES2079319B1 publication Critical patent/ES2079319B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Bacteriological culture medium and process for its preparation. This consists of the association of peptone-protease, extracts of meat and yeast, xylose, X-GAL, IPTG, bile salts, novobiocin, neutral red and agar agar in solution in deionized water, which is preserved in a refrigerator and used within 30 days following preparation. The preparation comprises the boiling of X-GAL in deionized water, the addition of peptone, xylose, bile salts and meat and yeast extracts, completing dissolution with the addition of further deionized water, neutral red to 0.1%, agar agar and finally sterile aqueous solutions of novobiocin and IPTG, placing the product in Petri dishes in which it is allowed to cool until it completely solidifies. Applicable to the isolation and identification of enteropathogenic bacteria, mainly Salmonella, Shigella and Aeromonas.
ES9401056A 1994-05-16 1994-05-16 BACTERIOLOGICAL CULTIVATION MEDIA AND PROCEDURE FOR ITS PREPARATION. Expired - Fee Related ES2079319B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9401056A ES2079319B1 (en) 1994-05-16 1994-05-16 BACTERIOLOGICAL CULTIVATION MEDIA AND PROCEDURE FOR ITS PREPARATION.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9401056A ES2079319B1 (en) 1994-05-16 1994-05-16 BACTERIOLOGICAL CULTIVATION MEDIA AND PROCEDURE FOR ITS PREPARATION.

Publications (2)

Publication Number Publication Date
ES2079319A1 true ES2079319A1 (en) 1996-01-01
ES2079319B1 ES2079319B1 (en) 1996-07-16

Family

ID=8286259

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9401056A Expired - Fee Related ES2079319B1 (en) 1994-05-16 1994-05-16 BACTERIOLOGICAL CULTIVATION MEDIA AND PROCEDURE FOR ITS PREPARATION.

Country Status (1)

Country Link
ES (1) ES2079319B1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081615A1 (en) * 2000-04-18 2001-11-01 Centro Nacional De Biopreparados Composition and method for detecting and early and differentiated counting of gram-negative microorganisms
WO2002000921A1 (en) * 2000-06-29 2002-01-03 Centro Nacional De Biopreparados Nutritional mixture and method for early identification and count of gram-negative organisms
WO2002020829A1 (en) * 2000-09-07 2002-03-14 Centro Nacional De Biopreparados Culture medium and method for identifying gram-negative microorganisms

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870601A (en) * 1973-05-04 1975-03-11 Schering Corp Novel diagnostic system for differentiation of enterobacteriaceae
US3893889A (en) * 1974-02-25 1975-07-08 Schering Corp Culture medium for differentiation of enterobacteriaceae

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870601A (en) * 1973-05-04 1975-03-11 Schering Corp Novel diagnostic system for differentiation of enterobacteriaceae
US3893889A (en) * 1974-02-25 1975-07-08 Schering Corp Culture medium for differentiation of enterobacteriaceae

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081615A1 (en) * 2000-04-18 2001-11-01 Centro Nacional De Biopreparados Composition and method for detecting and early and differentiated counting of gram-negative microorganisms
WO2002000921A1 (en) * 2000-06-29 2002-01-03 Centro Nacional De Biopreparados Nutritional mixture and method for early identification and count of gram-negative organisms
WO2002020829A1 (en) * 2000-09-07 2002-03-14 Centro Nacional De Biopreparados Culture medium and method for identifying gram-negative microorganisms

Also Published As

Publication number Publication date
ES2079319B1 (en) 1996-07-16

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