ES2077098T3 - Proteinas modificadas con sacaridos, solubles en agua. - Google Patents

Proteinas modificadas con sacaridos, solubles en agua.

Info

Publication number
ES2077098T3
ES2077098T3 ES91105361T ES91105361T ES2077098T3 ES 2077098 T3 ES2077098 T3 ES 2077098T3 ES 91105361 T ES91105361 T ES 91105361T ES 91105361 T ES91105361 T ES 91105361T ES 2077098 T3 ES2077098 T3 ES 2077098T3
Authority
ES
Spain
Prior art keywords
saccharide
water
soluble
saccharides
modified proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91105361T
Other languages
English (en)
Inventor
Wilhelm Dr Tischer
Joachim Prof Dr Rer Nat Klein
Rolf-Joachim Dr Rer Nat Muller
Stephan Dr Rer Nat Engelke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Roche Diagnostics GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH, Boehringer Mannheim GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Application granted granted Critical
Publication of ES2077098T3 publication Critical patent/ES2077098T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

UNA PROTEINA CONJUGADA SOLUBLE EN AGUA, CON SACARIDOS MODIFICADOS, VA UNIDA COVALENTEMENTE A TRAVES DE UN GRUPO DE SACARIDO A UN ENLACE FINAL DE HIDROCARBURO. TALES PROTEINAS CONJUGADAS CON SACARIDOS MODIFICADOS PUEDEN OBTENERSE DE FORMA SENCILLA DEL MODO SIGUIENTE: SE POLIMERIZA UN VINILSACARIDO Y EL POLI(VINILSACARIDO) ASI OBTENIDO SE CONJUGA CON LA PROTEINA, QUE PREFERENTEMENTE ES UNA ENZIMA, DE UN MODO EN SI CONOCIDO. LAS PROTEINAS CONJUGADAS SEGUN LA INVENCION SON ESTABLES, EN CASO DE ELEVADA ACTIVIDAD ENZIMATICA, DURANTE UN LARGO ESPACIO DE TIEMPO, INCLUSO EN SOLUCION ACUOSA, POR LO QUE RESULTAN ADECUADAS ESPECIALMENTE PARA KITS DE ENSAYO.
ES91105361T 1990-04-05 1991-04-04 Proteinas modificadas con sacaridos, solubles en agua. Expired - Lifetime ES2077098T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4011084A DE4011084A1 (de) 1990-04-05 1990-04-05 Saccharid-modifizierte, wasserloesliche proteine

Publications (1)

Publication Number Publication Date
ES2077098T3 true ES2077098T3 (es) 1995-11-16

Family

ID=6403857

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91105361T Expired - Lifetime ES2077098T3 (es) 1990-04-05 1991-04-04 Proteinas modificadas con sacaridos, solubles en agua.

Country Status (7)

Country Link
US (1) US5480790A (es)
EP (1) EP0450628B1 (es)
JP (1) JPH0791313B2 (es)
AT (1) ATE126523T1 (es)
DE (2) DE4011084A1 (es)
DK (1) DK0450628T3 (es)
ES (1) ES2077098T3 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2073511A1 (en) * 1990-11-14 1992-05-29 Matthew R. Callstrom Conjugates of poly(vinylsaccharide) with proteins for the stabilization of proteins
DE4231266A1 (de) * 1992-09-18 1994-03-24 Boehringer Mannheim Gmbh Dextran modifizierte Glutamat-Dehydrogenase
US6090925A (en) 1993-03-09 2000-07-18 Epic Therapeutics, Inc. Macromolecular microparticles and methods of production and use
US5981719A (en) 1993-03-09 1999-11-09 Epic Therapeutics, Inc. Macromolecular microparticles and methods of production and use
US6326479B1 (en) 1998-01-27 2001-12-04 Boston Probes, Inc. Synthetic polymers and methods, kits or compositions for modulating the solubility of same
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
AU4056700A (en) 1999-03-31 2000-10-16 Cornell Research Foundation Inc. Phosphatases with improved phytase activity
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
US6410643B1 (en) * 2000-03-09 2002-06-25 Surmodics, Inc. Solid phase synthesis method and reagent
EP1373573B1 (en) * 2001-03-09 2013-10-02 TrovaGene, Inc. Conjugate probes and optical detection of analytes
CA2465202C (en) * 2001-10-31 2014-01-21 Phytex, Llc Phytase-containing animal food and method
DE10200327A1 (de) * 2002-01-07 2003-07-17 Inst Technologie Der Kohlenhyd Verwendung von Copolymeren auf Basis von N-Vinyl-N'-Saccharidharnstoffmonomeren und von Polyvinylamiden für die Kultivierung von Stammzellen
ATE492631T1 (de) * 2002-09-13 2011-01-15 Cornell Res Foundation Inc Verwendung von mutationen zur verbesserung von aspergillus-phytasen
US20070025895A1 (en) * 2005-07-27 2007-02-01 Zunshe Qin Protein crystallography dialysis chamber that enables off-site high throughput cocktail screen
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
JP6272029B2 (ja) * 2010-09-15 2018-01-31 スリーエム イノベイティブ プロパティズ カンパニー 置換糖化合物及び歯科用組成物
US9522463B2 (en) * 2012-07-25 2016-12-20 Worktools Inc. Compact electric spring energized desktop stapler
EP3246002A1 (en) * 2016-05-20 2017-11-22 DENTSPLY DETREY GmbH Dental composition
KR20220116282A (ko) * 2019-12-20 2022-08-22 지니 바이오테크 유케이 리미티드 락톤 유도체의 합성 및 단백질 변형에서의 그 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3126759A1 (de) * 1981-07-07 1983-01-27 Boehringer Mannheim Gmbh, 6800 Mannheim Loesliche leber-uricase, verfahren zu ihrer herstellung und verwendung
DE3515586A1 (de) * 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
DE3540076A1 (de) * 1985-11-12 1987-05-14 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von creatinkinase
US5043436A (en) * 1986-11-20 1991-08-27 Kurita Water Ind., Ltd. Substrate for measurement of alpha-amylase activity
JPS63219787A (ja) * 1987-03-10 1988-09-13 旭エンジニアリング株式会社 削孔装置
JPH02219571A (ja) * 1989-02-20 1990-09-03 Kanebo Ltd 修飾プロテアーゼ及びその製造法

Also Published As

Publication number Publication date
US5480790A (en) 1996-01-02
DE59106243D1 (de) 1995-09-21
EP0450628A1 (de) 1991-10-09
EP0450628B1 (de) 1995-08-16
DE4011084A1 (de) 1991-10-10
ATE126523T1 (de) 1995-09-15
JPH0791313B2 (ja) 1995-10-04
JPH05137570A (ja) 1993-06-01
DK0450628T3 (da) 1996-01-02

Similar Documents

Publication Publication Date Title
ES2077098T3 (es) Proteinas modificadas con sacaridos, solubles en agua.
ATE68976T1 (de) Solubilisierung von proteinen fuer pharmazeutsche zusammensetzungen.
HUP0204544A2 (en) Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates
AU1316092A (en) Polyethylene protein conjugates
ATE303412T1 (de) Nicht antigen wirkende verzweigte polymerkonjugate
PT85777A (en) Process for producing recombinant pseudomonas exotoxin
TNSN93045A1 (fr) Nouveaux composes actifs
TR200100074T2 (tr) Nevrotrofik büyüme faktörü
EP1221052A4 (en) MARKER COMPLEXES OF CONJUGATED POLYMERS
ATE278418T1 (de) Kopplung unmodifizierter proteinen an haloacyl- oder dihaloacyl-derivatisierten polysacchariden zur herstellung von protein-polysaccharide impfstoffen
YU47886B (sh) Postupak za dobijanje novih sojeva s. avermitilis
AU2441188A (en) Heterobifunctional coupling agents
CA2055442A1 (en) Enzymes and their use in drug detection
EP0325270A3 (en) Anticancer conjugates
EP0378203A3 (de) Neue Cobalamin-Säurehydrazide und davon abgeleitete Cobalamin-Konjugate
Larsen et al. Macromolecular prodrugs I. Kinetics and mechanism of hydrolysis of O-benzoyl dextran conjugates in aqueous buffer and in human plasma
DK0752102T3 (da) Immunoenzymatisk konjugat, fremgangsmåde til fremstilling af konjugatet og anvendelse af dette.
Ito et al. Design and synthesis of a protein device that releases insulin in response to glucose concentration
Lloyd et al. Targeting and lysosomal handling of polymethacrylamide-oligopeptide conjugates
DK5288D0 (da) Vandoploeseligt polymerkonjugat, dets fremstilling og anvendelse
Abrams THE EFFECT OF IMMUNOENZYME COMPOSITION ON ENZYME-LINKED IMMUNOSORBENT ASSAY.
ATE122465T1 (de) Makromolekulare konjugate.
KR960031594A (ko) 재조합 인간 초-산화물 불균화효소(rhSOD)의 고분자 결합체 및 이를 제조하는 방법
KR890006810A (ko) 스트레팍스 및 이를 생성 하는 균주

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 450628

Country of ref document: ES