ES2070196T3 - Determinacion de la vitamina b12. - Google Patents

Determinacion de la vitamina b12.

Info

Publication number
ES2070196T3
ES2070196T3 ES90100449T ES90100449T ES2070196T3 ES 2070196 T3 ES2070196 T3 ES 2070196T3 ES 90100449 T ES90100449 T ES 90100449T ES 90100449 T ES90100449 T ES 90100449T ES 2070196 T3 ES2070196 T3 ES 2070196T3
Authority
ES
Spain
Prior art keywords
vitamin
receptors
phases
receptor
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90100449T
Other languages
English (en)
Inventor
Nicholas R Dr Hoyle
Gunter Dr Pappert
Michael Dr Grol
Christa Dr Hubner-Parajsz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2070196T3 publication Critical patent/ES2070196T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

PARA DETERMINAR VITAMINA B12, SE INCUBA UNA SOLUCION DE PRUEBA CON DOS O MAS RECEPTORES R1 Y R2, DE LOS QUE R1 FACILITA EL LIGAZON A LA FASE SOLIDA Y R2 LLEVA UNA MARCA. COMO UNO DE AMBOS RECEPTORES, SE UTILIZA UN RECEPTOR QUE DISPONE DE UN ANTICUERPO MONOCLONAL CON PODER AGLUTINANTE ESPECIFICO SOBRE B12 Y CON UNA CONSTANTE DE AFINIDAD PARA B12 DE AL MENOS 5X10 (POR) 9 (POR) L/MOL. EL OTRO RECEPTOR CONTIENE B12 O UNA SUSTANCIA ANALOGA, AISLA LAS FASES Y MIDE LA MARCA EN UNA DE AMBAS FASES.
ES90100449T 1989-01-11 1990-01-10 Determinacion de la vitamina b12. Expired - Lifetime ES2070196T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3900650A DE3900650A1 (de) 1989-01-11 1989-01-11 Vitamin-b12-bestimmung

Publications (1)

Publication Number Publication Date
ES2070196T3 true ES2070196T3 (es) 1995-06-01

Family

ID=6371888

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90100449T Expired - Lifetime ES2070196T3 (es) 1989-01-11 1990-01-10 Determinacion de la vitamina b12.

Country Status (8)

Country Link
US (2) US5451508A (es)
EP (1) EP0378197B1 (es)
JP (1) JP2750423B2 (es)
AT (1) ATE120552T1 (es)
AU (1) AU628460B2 (es)
CA (1) CA2007338A1 (es)
DE (2) DE3900650A1 (es)
ES (1) ES2070196T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187107A (en) * 1991-06-27 1993-02-16 Bio-Rad Laboratories, Inc. B12 enzyme imunoassay and sample pretreatment
DE4239815A1 (de) * 1992-11-26 1994-06-01 Boehringer Mannheim Gmbh Verbesserte B¶1¶¶2¶-Konjugate
GB9326238D0 (en) * 1993-12-23 1994-02-23 Sinvent As Method of assay
DE19739218A1 (de) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Aufreinigung von Substanzen aus einer biologischen Probe
US20010051346A1 (en) * 1998-09-18 2001-12-13 Erling Sundrehagen Assay
GB9820473D0 (en) * 1998-09-18 1998-11-11 Axis Biochemicals Asa Assay
DK1226153T3 (da) * 1999-10-26 2010-06-07 Univ Utah Res Found Phosphorescerende cobalaminer og anvendelser deraf
US20050158812A1 (en) * 2004-01-16 2005-07-21 Gomez Elizabeth A. Kits and methods for autoantibody detection
US8140141B2 (en) * 2006-05-17 2012-03-20 University Of Utah Research Foundation Devices and methods for fluorescent inspection and/or removal of material in a sample
CN102798726B (zh) * 2012-07-26 2015-09-23 博奥赛斯（天津）生物科技有限公司 维生素b12化学发光免疫定量检测试剂盒及其制备方法
CN103575914B (zh) * 2012-08-02 2016-08-03 北京勤邦生物技术有限公司 检测维生素b12的酶联免疫试剂盒及其应用
WO2021030166A1 (en) * 2019-08-09 2021-02-18 Siemens Healthcare Diagnostics Inc. Anti-hog tcn1 monoclonal antibodies and methods of production and use thereof
CN111808158B (zh) * 2020-07-23 2022-12-06 宁夏金维制药股份有限公司 一种维生素b12粗品的制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE326845B (es) * 1966-10-21 1970-08-03 Pharmacia Ab
US3981863A (en) 1975-02-25 1976-09-21 Micromedic Diagonistics, Inc. Cyanocobalamin derivatives
US4423154A (en) * 1978-06-26 1983-12-27 Becton Dickinson And Company Simultaneous radioassay of folate and vitamin B12
US4950612A (en) * 1987-12-16 1990-08-21 Microgenics Corporation Peroxy acid pretreatment in vitamin B12 assay
JPH03500003U (es) * 1988-06-15 1991-06-06
US5104815A (en) * 1988-10-11 1992-04-14 Abbott Laboratories Assay for cobalamins
DE3900648A1 (de) * 1989-01-11 1990-07-12 Boehringer Mannheim Gmbh Neue cobalamin-saeurehydrazide und davon abgeleitete cobalamin-konjugate
DE3900649A1 (de) * 1989-01-11 1990-07-12 Boehringer Mannheim Gmbh Verfahren zur abloesung eines analyten von seinem bindeprotein

Also Published As

Publication number Publication date
EP0378197A3 (de) 1991-10-23
EP0378197B1 (de) 1995-03-29
AU4768390A (en) 1990-07-19
EP0378197A2 (de) 1990-07-18
CA2007338A1 (en) 1990-07-11
ATE120552T1 (de) 1995-04-15
DE59008777D1 (de) 1995-05-04
JPH02259565A (ja) 1990-10-22
US5451508A (en) 1995-09-19
AU628460B2 (en) 1992-09-17
US5614394A (en) 1997-03-25
JP2750423B2 (ja) 1998-05-13
DE3900650A1 (de) 1990-07-12

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