ES2062955B1 - Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen. - Google Patents

Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen.

Info

Publication number
ES2062955B1
ES2062955B1 ES09300914A ES9300914A ES2062955B1 ES 2062955 B1 ES2062955 B1 ES 2062955B1 ES 09300914 A ES09300914 A ES 09300914A ES 9300914 A ES9300914 A ES 9300914A ES 2062955 B1 ES2062955 B1 ES 2062955B1
Authority
ES
Spain
Prior art keywords
polyhydroxyalkanoates
extraction
procedure
contain
halophilic bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES09300914A
Other languages
English (en)
Other versions
ES2062955A1 (es
Inventor
Escalona Antonio Munoz
Varela Francisco Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Repsol Quimica SA
Original Assignee
Repsol Quimica SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Repsol Quimica SA filed Critical Repsol Quimica SA
Priority to ES09300914A priority Critical patent/ES2062955B1/es
Priority to US08/234,325 priority patent/US5536419A/en
Priority to AT94500077T priority patent/ATE204907T1/de
Priority to EP94500077A priority patent/EP0622462B1/en
Priority to DE69428072T priority patent/DE69428072T2/de
Publication of ES2062955A1 publication Critical patent/ES2062955A1/es
Application granted granted Critical
Publication of ES2062955B1 publication Critical patent/ES2062955B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

PROCEDIMIENTO PARA LA EXTRACCION DE POLIHIDROXIALCANOATOS DE BACTERIAS HALOFILAS QUE LO CONTIENEN, MEDIANTE LA LISIS O ROTURA DE CELULAS HALOFILAS (POR EJEMPLO DEL TIPO DE LAS HALOBACTERIAS), QUE SE DESARROLLAN EN MEDIOS CON ELEVADAS CONCENTRACIONES DE SALES, POR CONCENTRACION POR CENTRIFUGACION Y POSTERIOR DILUCION-RESUSPENSION EN UN MEDIO DE BAJA CONCENTRACION DE SALES, POR EJEMPLO AGUA DULCE O DESTILADA Y POSTERIOR CENTRIFUGACION, SEDIMENTACION O FILTRACION DE LA SUSPENSION OBTENIDA.
ES09300914A 1993-04-29 1993-04-29 Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen. Expired - Lifetime ES2062955B1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES09300914A ES2062955B1 (es) 1993-04-29 1993-04-29 Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen.
US08/234,325 US5536419A (en) 1993-04-29 1994-04-28 Procedure for extraction of polyhydroxyalkanoates from halophilic bacteria which contain them
AT94500077T ATE204907T1 (de) 1993-04-29 1994-04-29 Verfahren zur extraktion von polyhydroxy- alkanoaten aus diese enthaltenden halophilen bakterien
EP94500077A EP0622462B1 (en) 1993-04-29 1994-04-29 Procedure for the extraction of polyhydroxyalkanoates from halophilic bacteria which contain them
DE69428072T DE69428072T2 (de) 1993-04-29 1994-04-29 Verfahren zur Extraktion von Polyhydroxy-Alkanoaten aus diese enthaltenden halophilen Bakterien

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09300914A ES2062955B1 (es) 1993-04-29 1993-04-29 Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen.

Publications (2)

Publication Number Publication Date
ES2062955A1 ES2062955A1 (es) 1994-12-16
ES2062955B1 true ES2062955B1 (es) 1995-06-16

Family

ID=8281647

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09300914A Expired - Lifetime ES2062955B1 (es) 1993-04-29 1993-04-29 Procedimiento para la extraccion de polihidroxialcanoatos de bacterias halofilas que lo contienen.

Country Status (5)

Country Link
US (1) US5536419A (es)
EP (1) EP0622462B1 (es)
AT (1) ATE204907T1 (es)
DE (1) DE69428072T2 (es)
ES (1) ES2062955B1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995033065A1 (en) * 1994-06-01 1995-12-07 The Procter & Gamble Company Process for recovering polyhydroxyalkanoates using centrifugal fractionation
CN1079835C (zh) * 1994-06-01 2002-02-27 普罗克特和甘保尔公司 利用空气分级作用回收聚羟基链烷酸酯的方法
DE19633475C2 (de) * 1996-08-20 2002-03-14 Buna Sow Leuna Olefinverb Gmbh Verfahren zur Gewinnung von Polyhydroxyalkanoaten und deren Verwendung
EP0975788A1 (en) * 1997-04-15 2000-02-02 Monsanto Company Methods of pha extraction and recovery using non-halogenated solvents
US6087471A (en) * 1997-04-15 2000-07-11 Monsanto Company High temperature PHA extraction using PHA-poor solvents
KR20010009719A (ko) * 1999-07-13 2001-02-05 성재갑 미생물로부터 폴리하이드록시알카노에이트를 분리하는 방법
CA2494322C (en) * 2002-08-06 2012-05-22 Metabolix Inc. Polymer extraction methods
US8283435B2 (en) * 2003-02-21 2012-10-09 Metabolix, Inc. PHA adhesive compositions
ATE518932T1 (de) * 2003-02-21 2011-08-15 Metabolix Inc Pha-mischungen
CN1813059A (zh) * 2003-05-02 2006-08-02 西格玛-奥尔德利希公司 固相细胞裂解和捕获平台
EP1687436B1 (en) * 2003-11-28 2009-01-07 PHB Industrial S.A. Process for recovering polyhydroxyalkanoates ("phas") from cellular biomass
WO2005059153A1 (fr) * 2003-12-19 2005-06-30 Tianan Biologic Material Co., Ltd. Ningbo Procede de separation, d'extraction et de purification de poly-$g(b)-hydroxyalcanoates (pha) directement a partir d'un milieu de culture bacterien fermente
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
TWI399345B (zh) * 2010-07-09 2013-06-21 Univ Nat Chi Nan Method for extracting polyhydroxyalkyl acid esters from waste sludge (1)
TWI399346B (zh) * 2010-07-09 2013-06-21 Univ Nat Chi Nan Extraction of Polyhydroxyalkyl Esters from Waste Sludge (2)
US9475930B2 (en) 2012-08-17 2016-10-25 Metabolix, Inc. Biobased rubber modifiers for polymer blends
IL222128A (en) * 2012-09-24 2017-12-31 Dr Nona Int Ltd The composition containing the extract of the lobacteria reduce tumors
IL222127B (en) 2012-09-24 2018-06-28 Dr Nona Int Ltd Topical halobactria extract composiiton for treating radiation skin tissue damage
EP3004225A1 (en) 2013-05-30 2016-04-13 Metabolix, Inc. Recyclate blends
CN106459544B (zh) 2014-03-27 2021-10-01 Cj 第一制糖株式会社 高度填充的聚合物体系
US20210403960A1 (en) * 2020-06-29 2021-12-30 The Regents Of The University Of California Systems and methods for producing polyhydroxyalkanoates from organic waste
KR20240093595A (ko) * 2021-10-20 2024-06-24 라모트 앳 텔-아비브 유니버시티 리미티드 고세균의 발효에 의한 pha의 제조방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1484478A (es) * 1965-06-24 1967-09-15
CH544809A (fr) * 1972-05-03 1973-11-30 Nestle Sa Procédé d'obtention de substances intracellulaires d'origine microbienne
US3871957A (en) * 1973-05-09 1975-03-18 Exxon Research Engineering Co Use of microorganisms to disperse and degrade oil spills
EP0036699B2 (en) * 1979-02-21 1987-09-02 Imperial Chemical Industries Plc Extraction of poly-beta-hydroxybutyric acid
EP0145233B2 (en) * 1983-11-23 1991-11-06 Imperial Chemical Industries Plc Separation processfor a 3-hydroxybutyrate polymer
NL8603073A (nl) * 1986-12-02 1988-07-01 Rijksuniversiteit Werkwijze voor het bereiden van polyesters door fermentatie; werkwijze voor het bereiden van optisch actieve carbonzuren en esters; polyester omvattende voortbrengselen.
DE3717902A1 (de) * 1987-05-27 1988-12-08 Wintershall Ag Verfahren zur abtrennung fester partikel verschiedener groesse aus viskosen fluessigkeiten
EP0295859B1 (en) * 1987-06-15 1994-11-17 Southern Cross Biotech Pty.Ltd. Production of proteins in active forms
CA1334430C (en) * 1989-04-06 1995-02-14 Claude Chavarie Separation of poly-.beta.-hydroxyalkanoic acids from microbial biomass
JPH0465425A (ja) * 1990-07-06 1992-03-02 Showa Denko Kk 共重合体及びその製造法
US5422257A (en) * 1991-11-29 1995-06-06 Firmenich Sa Method for obtaining poly-β-hydroxyoctanoic acid via solvent extraction

Also Published As

Publication number Publication date
EP0622462B1 (en) 2001-08-29
EP0622462A1 (en) 1994-11-02
ATE204907T1 (de) 2001-09-15
ES2062955A1 (es) 1994-12-16
US5536419A (en) 1996-07-16
DE69428072D1 (de) 2001-10-04
DE69428072T2 (de) 2002-05-02

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