ES2057061T3 - Procedimiento para la transferencia por conjugacion de vectores movilizables a partir de e.coli a bacterias gram-positivas, y vectores adecuados para ello. - Google Patents

Procedimiento para la transferencia por conjugacion de vectores movilizables a partir de e.coli a bacterias gram-positivas, y vectores adecuados para ello.

Info

Publication number
ES2057061T3
ES2057061T3 ES89120473T ES89120473T ES2057061T3 ES 2057061 T3 ES2057061 T3 ES 2057061T3 ES 89120473 T ES89120473 T ES 89120473T ES 89120473 T ES89120473 T ES 89120473T ES 2057061 T3 ES2057061 T3 ES 2057061T3
Authority
ES
Spain
Prior art keywords
vectors
coli
procedure
transfer
conjugation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89120473T
Other languages
English (en)
Inventor
Andreas Schafer
Jorn Kalinowski
Alfred Prof Puhler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Degussa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa GmbH filed Critical Degussa GmbH
Application granted granted Critical
Publication of ES2057061T3 publication Critical patent/ES2057061T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

EL INVENTO SE REFIERE A UN PROCEDIMIENTO PARA LA TRANSFERENCIA CONJUGATIVA DE VECTORES MOVILIZABLES PROCEDENTES DE MOVILIZADORES E COLI EN BACTERIAS GRAM POSITIVA Y VECTORES APROPIADOS. LOS VECTORES APROPIADOS QUE SE REPLICAN EN BASES E COLI SE MODIFICAN A TRAVES DE LA INSERCION EN EL LADO INVISIBLE DE UNOS PLASMIDOS EN UNA ACTIVA, MIENTRAS QUE EN LA BASE E COLI UTILIZABLE COMO MOVILIZADOR ESTA PRESENTE UN PLASMIDO CUYA FUNCION TRANSFERACTIVA SOBRE EL LADO DEL MOVILIZADOR MENCIONADO.
ES89120473T 1988-12-09 1989-11-06 Procedimiento para la transferencia por conjugacion de vectores movilizables a partir de e.coli a bacterias gram-positivas, y vectores adecuados para ello. Expired - Lifetime ES2057061T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3841453A DE3841453A1 (de) 1988-12-09 1988-12-09 Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren

Publications (1)

Publication Number Publication Date
ES2057061T3 true ES2057061T3 (es) 1994-10-16

Family

ID=6368766

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89120473T Expired - Lifetime ES2057061T3 (es) 1988-12-09 1989-11-06 Procedimiento para la transferencia por conjugacion de vectores movilizables a partir de e.coli a bacterias gram-positivas, y vectores adecuados para ello.

Country Status (7)

Country Link
US (1) US5346818A (es)
EP (1) EP0372230B1 (es)
JP (1) JPH0829094B2 (es)
AU (1) AU626953B2 (es)
DE (2) DE3841453A1 (es)
ES (1) ES2057061T3 (es)
NO (1) NO894345L (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0501921T3 (da) * 1991-03-01 2001-10-15 Syngenta Participations Ag Fremgangsmåde til genetisk manipulering af myxobakterier
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
DE4208785A1 (de) * 1992-03-19 1993-09-23 Degussa Verfahren zum auffinden von insertionselementen (is-elemente) oder transposonen
EP0817856A1 (en) * 1995-03-22 1998-01-14 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
JP2001508282A (ja) * 1996-01-19 2001-06-26 ノボ ノルディスク バイオテック，インコーポレイティド 接合において有用な細菌ドナー細胞
US5763187A (en) * 1997-01-17 1998-06-09 Novo Nordisk Biotech, Inc. Bacterial donor cell useful in conjugation
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10137815A1 (de) * 2001-08-06 2003-02-27 Basf Ag Verfahren zur Herstellung eines marker-freien mutierten Zielorganismus sowie dafür geeignete Plasmidvektoren
US6524147B1 (en) * 2001-09-28 2003-02-25 Mark X Steering Systems, Llc Power assist marine steering system
WO2005010144A2 (en) * 2003-04-21 2005-02-03 Wisconsin Alumni Research Foundation Displacing a plasmid in a bacterial population
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
FR3099769B1 (fr) 2019-08-05 2021-11-05 Bgene Genetics Mutagénèse bactérienne par conjugaison en milieu liquide
CN117280036A (zh) * 2021-05-25 2023-12-22 丝芭博株式会社 质粒的制备方法及质粒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL67510A (en) * 1981-12-17 1988-08-31 Kyowa Hakko Kogyo Kk Recombinant vector plasmids autonomously replicable in microorganisms belonging to the genus corynebacterium or brevibacterium and process for the production thereof
JPS60192586A (ja) * 1984-03-12 1985-10-01 Kyowa Hakko Kogyo Co Ltd 制限欠損変異株

Also Published As

Publication number Publication date
DE58904497D1 (de) 1993-07-01
NO894345D0 (no) 1989-11-01
JPH02273188A (ja) 1990-11-07
EP0372230A3 (de) 1991-09-04
EP0372230A2 (de) 1990-06-13
NO894345L (no) 1990-06-11
US5346818A (en) 1994-09-13
AU626953B2 (en) 1992-08-13
EP0372230B1 (de) 1993-05-26
DE3841453A1 (de) 1990-06-13
AU4608689A (en) 1990-06-14
JPH0829094B2 (ja) 1996-03-27

Similar Documents

Publication Publication Date Title
ES2057061T3 (es) Procedimiento para la transferencia por conjugacion de vectores movilizables a partir de e.coli a bacterias gram-positivas, y vectores adecuados para ello.
DE60313846D1 (de) Intrakapsulares, akkomodatives implantat
IT1314857B1 (it) Convogliatore radioelettrico asimmetrico terapeutico.
DK0510949T3 (da) Cytomoduleret konjugat indeholdende specifikke bindingspar.
CY1119833T1 (el) Ομαδες αδενοσχετιζομενου ιου (aav), αλληλουχιες, φορεις που τις περιεχουν και χρησεις αυτων
CL2003002786A1 (es) Composicion liofilizada que comprende i) una epotilona y ii) manitol o una ciclodextrina y composicion liofilizada que los comprende reconstituida con solvente, util como citotoxico en el tratamiento de tumores.
HK1097727A1 (en) Heating cushion and device comprising such a cushion
ES2067432T1 (es) Embolo de amortiguador de piezas desiguales, insertadas.
MX171493B (es) Fibras de poli(vinil pirrolidona) para-aramida y procedimiento para su fabricacion
ES2174138T3 (es) Dispositivo para el movimiento de un cuerpo en el espacio.
DE50210940D1 (de) Blasenkatheter
ES2194301T3 (es) Aparato para depilar con rodillo giratorio.
AR017368A1 (es) Un procedimiento para la insercion de adn foraneo en una celula hospedante, un vector recombinante utilizado en dicho procedimiento y el uso del mismo
ES2169575T3 (es) Soporte de datos con un estado fundamental reproducible de datos basicos y procedimiento para su realizacion.
ES2115424T3 (es) Derivados prolin-amidicos basicos de los antibioticos ge 2270 y similares a ge 2270.
BR0004547A (pt) Pistão de compressor do tipo prato oscilante cuja seção da extremidade é formada de um material diferente daquele da parte de acoplamento do prato oscilante
CO5070700A1 (es) Genes novedosos de (adp-ribosa) polimerasa
MXPA03003798A (es) Derivados triciclicos de indol con actividad antiangiogenica.
NO873919D0 (no) Kloning og anvendelse av transaminasegenet tyrb.
AR020141A1 (es) Toxinas y genes pesticidas de cepas de bacillus laterosporus
ES2057897T3 (es) Vector lanzadera para el desarrollo de cepas de bacillus thuringiensis recombinantes-i.
BR0016616A (pt) Microrganismos atenuados para o tratamento de infecção
MX9401052A (es) Compuestos novedosos de piperidina-triazina, adecuados para utilizarse como estabilizantes para materiales organicos.
ES2176561T3 (es) Soporte de clavija para una sujecion elastica de un amortiguador de golpes.
ES2070090B1 (es) Protector integral de la columna vertebral.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 372230

Country of ref document: ES