ES2050277T3 - Metodo de ensayo para un ligando en una muestra. - Google Patents

Metodo de ensayo para un ligando en una muestra.

Info

Publication number
ES2050277T3
ES2050277T3 ES89909006T ES89909006T ES2050277T3 ES 2050277 T3 ES2050277 T3 ES 2050277T3 ES 89909006 T ES89909006 T ES 89909006T ES 89909006 T ES89909006 T ES 89909006T ES 2050277 T3 ES2050277 T3 ES 2050277T3
Authority
ES
Spain
Prior art keywords
binding partner
specific binding
ligand
sample
optical structure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89909006T
Other languages
English (en)
Inventor
John Attridge
Ian Alexander Shanks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARS HOLDING 89 NV
Original Assignee
ARS HOLDING 89 NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ARS HOLDING 89 NV filed Critical ARS HOLDING 89 NV
Application granted granted Critical
Publication of ES2050277T3 publication Critical patent/ES2050277T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Examining Or Testing Airtightness (AREA)

Abstract

Un método de ensayo de un ligando en una muestra, el cual método comprende las etapas de (a) incubar la muestra en contacto con un compañero de unión específica para el ligando que se desea detectar, estando dicho compañero de unión específica soportado sobre una superficie de una estructura óptica y en presencia de otro reactivo que es o bienun análogo del ligando marcado fluorescentemente o fosforescentemente, específico para el mismo compañero de unión específica, o bienotro compañero de unión específica marcado uorescentemente o fosforescentemente, para el ligando que se desea detectar, comprendiendo dicha estructura óptica un sustrato, una capa de metal, e, interpuesta entre ellas, una capa de material dieléctrico que tiene un índice de refracción inferior al de dicho sustrato, estando dicho compañero de unión específica directa o indirectamente adsorbido sobre o unido a dicha capa de metal; (b) irradiar otra superficie de dicha estructura óptica a un ángulo o margen de ángulos respecto de la normal adecuado, de forma que tenga lugar resonancia de plasmón de superficie de largo alcance y de forma que se genere fluorescencia o fosforescencia; y (c) analizar la fluorescencia o fosforescencia generada para determinar si está alterada por la formación de complejo, y, si se desea, en qué cuantía y a qué velocidad tiene esto lugar.
ES89909006T 1988-07-25 1989-07-25 Metodo de ensayo para un ligando en una muestra. Expired - Lifetime ES2050277T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB888817710A GB8817710D0 (en) 1988-07-25 1988-07-25 Method of assay

Publications (1)

Publication Number Publication Date
ES2050277T3 true ES2050277T3 (es) 1994-05-16

Family

ID=10641075

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89909006T Expired - Lifetime ES2050277T3 (es) 1988-07-25 1989-07-25 Metodo de ensayo para un ligando en una muestra.

Country Status (11)

Country Link
EP (2) EP0353937A1 (es)
JP (1) JP2969177B2 (es)
AU (1) AU638938B2 (es)
CA (1) CA1339005C (es)
DE (1) DE68914134T2 (es)
ES (1) ES2050277T3 (es)
GB (1) GB8817710D0 (es)
IL (1) IL91105A0 (es)
NO (1) NO177407C (es)
WO (1) WO1990001166A1 (es)
ZA (1) ZA895648B (es)

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NL9001052A (nl) * 1990-05-02 1991-12-02 Tno Werkwijze voor het bepalen van een specifiek ligand in een vloeibaar monster met behulp van een evanescent veld, alsook een daarvoor geschikt onderdeel van de benodigde meetinrichting.
DE4024476C1 (es) * 1990-08-02 1992-02-27 Boehringer Mannheim Gmbh, 6800 Mannheim, De
GB9102646D0 (en) * 1991-02-07 1991-03-27 Fisons Plc Analytical device
DE69110032T2 (de) * 1991-06-08 1995-12-21 Hewlett Packard Gmbh Verfahren und Gerät zur Feststellung und/oder Konzentrationsbestimmung von Biomolekülen.
EP0575132A1 (en) * 1992-06-17 1993-12-22 Hewlett-Packard Company Optical measuring device
SE9301270D0 (sv) 1993-04-19 1993-04-17 Biosensor
US5919712A (en) 1993-05-18 1999-07-06 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
US5677196A (en) * 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
US5395587A (en) * 1993-07-06 1995-03-07 Smithkline Beecham Corporation Surface plasmon resonance detector having collector for eluted ligate
US5814565A (en) * 1995-02-23 1998-09-29 University Of Utah Research Foundation Integrated optic waveguide immunosensor
US6611634B2 (en) 1996-03-19 2003-08-26 University Of Utah Research Foundation Lens and associatable flow cell
US5776785A (en) * 1996-12-30 1998-07-07 Diagnostic Products Corporation Method and apparatus for immunoassay using fluorescent induced surface plasma emission
DE19715483A1 (de) 1997-04-14 1998-10-15 Boehringer Mannheim Gmbh Methode zur gleichzeitigen Bestimmung von biomolekularen Wechselwirkungen mittels Plasmonenresonanz undFluoreszenzdetektion
US6222619B1 (en) 1997-09-18 2001-04-24 University Of Utah Research Foundation Diagnostic device and method
DE102004027957A1 (de) * 2004-06-08 2005-12-29 Carl Zeiss Jena Gmbh Verfahren und Anordnung zur Untersuchung der Wechselwirkung von Biomolekülen
US20070177150A1 (en) * 2006-01-27 2007-08-02 Gruhlke Russell W Surface plasmon resonance biosensor using coupled surface plasmons to decrease width of reflectivity dip
EP1818668A1 (en) * 2006-02-09 2007-08-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Long range surface plasmon fluorescence spectroscopy for bio-affinity studies
JP2008102117A (ja) * 2006-09-21 2008-05-01 Fujifilm Corp 表面プラズモン増強蛍光センサおよび蛍光検出方法
CN109073642A (zh) 2015-09-17 2018-12-21 格哈德·马勒 用于生物感测和其它应用的传感器设备

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3277030D1 (en) * 1981-09-18 1987-09-24 Battelle Memorial Institute Method and apparatus for the determination of species in solution with an optical wave-guide
WO1986000135A1 (en) * 1984-06-13 1986-01-03 Unilever Plc Photometric instruments, their use in methods of optical analyses, and ancillary devices therefor
GB8415018D0 (en) * 1984-06-13 1984-07-18 Unilever Plc Chemical test procedures
GB8509491D0 (en) * 1985-04-12 1985-05-15 Plessey Co Plc Optic waveguide biosensors
US4649280A (en) 1985-05-10 1987-03-10 The University Of Rochester Method and system for the enhancement of fluorescence
GB2185308B (en) 1986-01-10 1989-10-25 Stc Plc Optical sensor device
GB8705650D0 (en) * 1987-03-10 1987-04-15 Pa Consulting Services Assay technique
NL8700851A (nl) * 1987-04-10 1988-11-01 Tno Werkwijze en inrichting voor het detecteren van zeer lage concentraties van een in een meetmedium aanwezige chemische component onder toepassing van oppervlakte-plasmonresonantie en elektrochemisch gestimuleerde adsorptie.
CA1321488C (en) * 1987-08-22 1993-08-24 Martin Francis Finlan Biological sensors
NL8701998A (nl) * 1987-08-26 1989-03-16 Univ Leiden Werkwijze voor het door hydrogenolyse ontleden van chemische afvalstoffen, in het bijzonder organische halogeenverbindingen.
GB8813307D0 (en) * 1988-06-06 1988-07-13 Amersham Int Plc Biological sensors

Also Published As

Publication number Publication date
AU4043389A (en) 1990-02-19
GB8817710D0 (en) 1988-09-01
NO177407B (no) 1995-05-29
JPH03502604A (ja) 1991-06-13
DE68914134D1 (de) 1994-04-28
EP0382832A1 (en) 1990-08-22
EP0353937A1 (en) 1990-02-07
NO901349D0 (no) 1990-03-23
EP0382832B1 (en) 1994-03-23
CA1339005C (en) 1997-03-25
NO177407C (no) 1995-09-06
IL91105A0 (en) 1990-03-19
NO901349L (no) 1990-05-23
JP2969177B2 (ja) 1999-11-02
AU638938B2 (en) 1993-07-15
WO1990001166A1 (en) 1990-02-08
ZA895648B (en) 1990-06-27
DE68914134T2 (de) 1994-08-11

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