ES2034037T3 - Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero. - Google Patents

Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero.

Info

Publication number
ES2034037T3
ES2034037T3 ES198888109819T ES88109819T ES2034037T3 ES 2034037 T3 ES2034037 T3 ES 2034037T3 ES 198888109819 T ES198888109819 T ES 198888109819T ES 88109819 T ES88109819 T ES 88109819T ES 2034037 T3 ES2034037 T3 ES 2034037T3
Authority
ES
Spain
Prior art keywords
lipase
serum
determination
turbidimetric method
microns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198888109819T
Other languages
English (en)
Inventor
Jack B. Robinson Jr.
Robert Saul Levinson
Hillard W. Johnson
Donald L. Mowles
Herman Swopes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2034037T3 publication Critical patent/ES2034037T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G01N2333/92—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

REACTIVO PARA LA DETERMINACION DE LIPASA DEL SUERO QUE COMPRENDE PARTICULAS DE LIPIDOS QUE TIENEN UN DIAMETRO DE MENOS DE 0,5 MICRAS. EL REACTIVO TIENE ESTABILIDAD MEJORADA FRENTE A LA SEDIMENTACION Y LA COALESCENCIA. TAMBIEN TRATA LA INVENCION DE UN METODO TURBIDIMETRICO PARA LA DETERMINACION DE LIPASA DEL SUERO EN LA QUE UNA MUESTRA DE SUERO SE AÑADE A UNA SUSPENSION DE PARTICULAS LIPIDICAS QUE TIENEN UN DIAMETRO DE MENOS DE 0,5 MICRAS Y LA CANTIDAD DE LIPASA PRESENTE EN LA MUESTRA DE SUERO SE DETERMINA MEDIENTE MEDIDA DE UN CAMBIO EN LA TURBIDEZ DE LA SUSPENSION.
ES198888109819T 1987-07-01 1988-06-21 Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero. Expired - Lifetime ES2034037T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6897587A 1987-07-01 1987-07-01

Publications (1)

Publication Number Publication Date
ES2034037T3 true ES2034037T3 (es) 1993-04-01

Family

ID=22085894

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198888109819T Expired - Lifetime ES2034037T3 (es) 1987-07-01 1988-06-21 Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero.

Country Status (8)

Country Link
EP (1) EP0297387B1 (es)
JP (1) JPS6430600A (es)
KR (1) KR890002666A (es)
AT (1) ATE79137T1 (es)
AU (1) AU612661B2 (es)
CA (1) CA1320899C (es)
DE (1) DE3873437T2 (es)
ES (1) ES2034037T3 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248598A (en) * 1989-05-25 1993-09-28 Ivan E. Modrovich Lipase single reagent system
FR2696193B1 (fr) * 1992-09-25 1994-12-09 Interfacial Technology Concept Procédé de mesure de l'activité des phospholipases.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3436187A (en) * 1965-03-03 1969-04-01 Dade Reagents Inc Method of estimating thymol turbidity employing polystyrene latex suspensions as standards
DE2904305C2 (de) * 1979-02-05 1981-07-02 Boehringer Mannheim Gmbh, 6800 Mannheim Reagens zur Lipasebestimmung und Verfahren zu seiner Herstellung
EP0021572A1 (en) * 1979-06-04 1981-01-07 American Hospital Supply Corporation A substrate for use in a turbidimetric assay for lipase and a method for making this substrate
DE3365307D1 (en) * 1983-09-07 1986-09-18 American Hospital Supply Corp Reconstitutable dry reagent for diagnostic purposes and method of manufacture

Also Published As

Publication number Publication date
ATE79137T1 (de) 1992-08-15
EP0297387A3 (en) 1989-05-03
DE3873437T2 (de) 1992-12-03
AU1855388A (en) 1989-01-05
KR890002666A (ko) 1989-04-11
JPS6430600A (en) 1989-02-01
EP0297387B1 (en) 1992-08-05
EP0297387A2 (en) 1989-01-04
AU612661B2 (en) 1991-07-18
CA1320899C (en) 1993-08-03
DE3873437D1 (de) 1992-09-10

Similar Documents

Publication Publication Date Title
IL74303A (en) Compositions and test device for determining the presence of leukocytes,esterase and protease in biological samples
DE68923907D1 (de) Verfahren zur immunochromatographischen Analyse.
DE3671534D1 (de) Verfahren zum nachweis von agglutinierten erythrozyten.
DE3669206D1 (de) Verfahren zur quantifizierung von zellpopulationen bzw. subpopulationen sowie hierfuer geeignetes reagenz.
ES2101802T3 (es) Ensayo para determinar los niveles de igf-i, igf-ii y gh libres en liquidos corporales.
ES2033266T3 (es) Procedimiento y estuche de ensayo para la determinacion de sustancias activas libres en liquidos biologicos.
SE9403078D0 (sv) Milk assay
ES2034037T3 (es) Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero.
EP0309882A3 (de) Verfahren zur spezifischen Bestimmung des Serumfructosamingehalts sowie hierfür geeignetes Reagenzgemisch
DE69321520D1 (de) Verfahren und Reagenz zur Bestimmung von Fructosamin
ATE77487T1 (de) Immunologisches bestimmungsverfahren zur bestimmung von hapteneigenschaften aufweisenden freien substanzen.
ATE80665T1 (de) Verfahren und reagens zur bestimmung von ld-1isoenzym.
FR2424536A1 (fr) Procede de determination des acides biliaires dans les fluides biologiques
CA1268705A (en) Digoxin assay
ATE78342T1 (de) Verfahren zur feststellung von schwangerschaftsstoerungen.
KR940004326A (ko) 티록신 또는 트리요오도티로닌의 측정을 위한 방법 및 표준용액
DE3587845T2 (de) Verfahren zur Bestimmung eines Liganden.
Furukawa et al. Double-stranded DNA inhibits cardiolipin-binding activity in lepromatous leprosy patients' sera.
FR2645963B3 (fr) Procede et dispositif pour la determination potentiometrique de la concentration d'ions azoture ou d'acide azothydrique
ATE119670T1 (de) Nichtspezifische adsorptionsfreie methode zur bestimmung eines antikörpers und bestimmungsreagentien.
FR2689246B1 (fr) Procede immunochimique de detection de l'origine d'une substance produite par genie genetique et reactifs pour sa mise en óoeuvre.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 297387

Country of ref document: ES