ES2034037T3 - Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero. - Google Patents
Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero.Info
- Publication number
- ES2034037T3 ES2034037T3 ES198888109819T ES88109819T ES2034037T3 ES 2034037 T3 ES2034037 T3 ES 2034037T3 ES 198888109819 T ES198888109819 T ES 198888109819T ES 88109819 T ES88109819 T ES 88109819T ES 2034037 T3 ES2034037 T3 ES 2034037T3
- Authority
- ES
- Spain
- Prior art keywords
- lipase
- serum
- determination
- turbidimetric method
- microns
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/918—Carboxylic ester hydrolases (3.1.1)
- G01N2333/92—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
REACTIVO PARA LA DETERMINACION DE LIPASA DEL SUERO QUE COMPRENDE PARTICULAS DE LIPIDOS QUE TIENEN UN DIAMETRO DE MENOS DE 0,5 MICRAS. EL REACTIVO TIENE ESTABILIDAD MEJORADA FRENTE A LA SEDIMENTACION Y LA COALESCENCIA. TAMBIEN TRATA LA INVENCION DE UN METODO TURBIDIMETRICO PARA LA DETERMINACION DE LIPASA DEL SUERO EN LA QUE UNA MUESTRA DE SUERO SE AÑADE A UNA SUSPENSION DE PARTICULAS LIPIDICAS QUE TIENEN UN DIAMETRO DE MENOS DE 0,5 MICRAS Y LA CANTIDAD DE LIPASA PRESENTE EN LA MUESTRA DE SUERO SE DETERMINA MEDIENTE MEDIDA DE UN CAMBIO EN LA TURBIDEZ DE LA SUSPENSION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6897587A | 1987-07-01 | 1987-07-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2034037T3 true ES2034037T3 (es) | 1993-04-01 |
Family
ID=22085894
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES198888109819T Expired - Lifetime ES2034037T3 (es) | 1987-07-01 | 1988-06-21 | Un metodo turbidimetrico mejorado para la determinacion de lipasa en suero. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0297387B1 (es) |
JP (1) | JPS6430600A (es) |
KR (1) | KR890002666A (es) |
AT (1) | ATE79137T1 (es) |
AU (1) | AU612661B2 (es) |
CA (1) | CA1320899C (es) |
DE (1) | DE3873437T2 (es) |
ES (1) | ES2034037T3 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5248598A (en) * | 1989-05-25 | 1993-09-28 | Ivan E. Modrovich | Lipase single reagent system |
FR2696193B1 (fr) * | 1992-09-25 | 1994-12-09 | Interfacial Technology Concept | Procédé de mesure de l'activité des phospholipases. |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3436187A (en) * | 1965-03-03 | 1969-04-01 | Dade Reagents Inc | Method of estimating thymol turbidity employing polystyrene latex suspensions as standards |
DE2904305C2 (de) * | 1979-02-05 | 1981-07-02 | Boehringer Mannheim Gmbh, 6800 Mannheim | Reagens zur Lipasebestimmung und Verfahren zu seiner Herstellung |
EP0021572A1 (en) * | 1979-06-04 | 1981-01-07 | American Hospital Supply Corporation | A substrate for use in a turbidimetric assay for lipase and a method for making this substrate |
EP0134291B1 (de) * | 1983-09-07 | 1986-08-13 | American Hospital Supply Corporation | Rekonstituierbares Trockenreagenz für diagnostische Zwecke und Verfahren zu seiner Herstellung |
-
1988
- 1988-06-21 ES ES198888109819T patent/ES2034037T3/es not_active Expired - Lifetime
- 1988-06-21 AT AT88109819T patent/ATE79137T1/de not_active IP Right Cessation
- 1988-06-21 DE DE8888109819T patent/DE3873437T2/de not_active Expired - Fee Related
- 1988-06-21 EP EP88109819A patent/EP0297387B1/en not_active Expired - Lifetime
- 1988-06-30 KR KR1019880008142A patent/KR890002666A/ko not_active Application Discontinuation
- 1988-06-30 CA CA000570812A patent/CA1320899C/en not_active Expired - Fee Related
- 1988-06-30 AU AU18553/88A patent/AU612661B2/en not_active Ceased
- 1988-06-30 JP JP63164101A patent/JPS6430600A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP0297387A3 (en) | 1989-05-03 |
DE3873437D1 (de) | 1992-09-10 |
CA1320899C (en) | 1993-08-03 |
DE3873437T2 (de) | 1992-12-03 |
ATE79137T1 (de) | 1992-08-15 |
AU612661B2 (en) | 1991-07-18 |
EP0297387A2 (en) | 1989-01-04 |
KR890002666A (ko) | 1989-04-11 |
EP0297387B1 (en) | 1992-08-05 |
AU1855388A (en) | 1989-01-05 |
JPS6430600A (en) | 1989-02-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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