ES2031065T3 - Procedimiento para aumentar el rendimiento cuantico en la oxidacion de luminol mediante peroxidos en presencia de peroxidasa. - Google Patents

Procedimiento para aumentar el rendimiento cuantico en la oxidacion de luminol mediante peroxidos en presencia de peroxidasa.

Info

Publication number
ES2031065T3
ES2031065T3 ES198686117749T ES86117749T ES2031065T3 ES 2031065 T3 ES2031065 T3 ES 2031065T3 ES 198686117749 T ES198686117749 T ES 198686117749T ES 86117749 T ES86117749 T ES 86117749T ES 2031065 T3 ES2031065 T3 ES 2031065T3
Authority
ES
Spain
Prior art keywords
quantum yield
peroxidase
fluorescein
luminol
peroxides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686117749T
Other languages
English (en)
Inventor
Karl Dr. Wulff
Marin Dr. Gerber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2031065T3 publication Critical patent/ES2031065T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)

Abstract

PARA AUMENTAR EL PRODUCTO CUANTICO MEDIANTE LA OXIDACION DE LUMINOL, O 7-DIALQUILAMINONAFTALIN-1-2 DICARBONACIDO-HIDRACIDO, DENTRO DEL GRUPO ALQUILO QUE CONTIENE DE 1 A 3 ATOMOS DE CARBONO, A UNIONES PEROXIDO EN PRESENCIA DE PEROXIDASA (POD) SE DESARROLLA LA TRANSFORMACION EN PRESENCIA DE FLUORESCINA DENTRO DE UN CORTO RANGO DE CONCENTRACION, EN EL CUAL A UN PRODUCTO DADO, SEA MAYOR QUE LA SUMA DE LOS PRODUCTOS CUANTICOS DE UN UNICO MATERIAL QUIMIOLUMINISCENTE. UN REACTIVO APROPIADO PARA ESTA FUNCION CONTIENE LUMINOL O 7-CARBONO POR GRUPO ALQUILO, FLUORESCEINA, UN PROVEEDOR DE HIDROGENO, UNA SUSTANCIA TAMPON (PH = 7,5 HASTA 9) Y EVENTUALMENTE UN MEDIO SECUESTRADOR.
ES198686117749T 1985-12-20 1986-12-19 Procedimiento para aumentar el rendimiento cuantico en la oxidacion de luminol mediante peroxidos en presencia de peroxidasa. Expired - Lifetime ES2031065T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853545398 DE3545398A1 (de) 1985-12-20 1985-12-20 Verfahren zur steigerung der quanten-ausbeute bei der oxidation von luminol durch peroxide in gegenwart von peroxidase

Publications (1)

Publication Number Publication Date
ES2031065T3 true ES2031065T3 (es) 1992-12-01

Family

ID=6289141

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686117749T Expired - Lifetime ES2031065T3 (es) 1985-12-20 1986-12-19 Procedimiento para aumentar el rendimiento cuantico en la oxidacion de luminol mediante peroxidos en presencia de peroxidasa.

Country Status (9)

Country Link
US (1) US4834918A (es)
EP (1) EP0228046B1 (es)
JP (1) JPH066074B2 (es)
AT (1) ATE74209T1 (es)
DE (2) DE3545398A1 (es)
DK (1) DK612186A (es)
ES (1) ES2031065T3 (es)
FI (1) FI84519C (es)
ZA (1) ZA869544B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552298A (en) * 1992-10-23 1996-09-03 Lumigen, Inc. Enzyme-catalyzed chemiluminescence from hydroxyaryl cyclic diacylhydrazide compounds
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
DE19623814C2 (de) * 1995-06-15 1999-02-11 Lab Molecular Biophotonics Verfahren und Vorrichtung zur analytischen Bestimmung von 5-Hydroxyindolen oder Catecholaminen
GB9514594D0 (en) * 1995-07-17 1995-09-13 Johnson & Johnson Clin Diag Chemiluminescent analytical method
US7799573B2 (en) 2005-08-31 2010-09-21 Normadics, Inc. Detection of explosives and other species
WO2008121124A1 (en) 2007-03-19 2008-10-09 Nomadics, Inc. Hydrogen peroxide detector and methods
EP2443462A1 (en) * 2009-06-16 2012-04-25 Martin Jan Peter Eversdijk Methods for detecting the presence or absence of blood
KR101099508B1 (ko) 2010-06-30 2011-12-27 중앙대학교 산학협력단 과붕산염 선택성을 갖는 플루오레세인계 화합물을 포함하는 센서 및 이를 이용한 과붕산염 검출방법
CN101936911B (zh) * 2010-08-06 2012-07-04 陕西师范大学 具有长波长化学发光及荧光和显色功能的血迹检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE7811630L (sv) * 1977-11-17 1979-05-18 Welsh Nat School Med Metod for uppteckt eller mengdbestemning av substanser med anvendning av merkningsteknik
US4277437A (en) * 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4709016A (en) * 1982-02-01 1987-11-24 Northeastern University Molecular analytical release tags and their use in chemical analysis
EP0087959B1 (en) * 1982-03-03 1985-09-25 National Research Development Corporation Enhanced luminescent and luminometric assay
JPS58158542A (ja) * 1982-03-17 1983-09-20 Hitachi Ltd 化学発光増感法
EP0116454B1 (en) * 1983-02-11 1987-04-29 National Research Development Corporation Enhanced luminescent or luminometric assay
AU582357B2 (en) * 1984-04-06 1989-03-23 Minnesota Mining And Manufacturing Company Chemiluminescent methods and kit
GB8420053D0 (en) * 1984-08-07 1984-09-12 Secr Social Service Brit Enhanced luminescent/luminometric assay

Also Published As

Publication number Publication date
FI84519B (fi) 1991-08-30
DE3684577D1 (de) 1992-04-30
JPS62156546A (ja) 1987-07-11
JPH066074B2 (ja) 1994-01-26
ZA869544B (en) 1987-08-26
FI865166A0 (fi) 1986-12-17
DK612186D0 (da) 1986-12-18
US4834918A (en) 1989-05-30
FI84519C (fi) 1991-12-10
EP0228046A3 (en) 1988-09-07
DE3545398A1 (de) 1987-06-25
EP0228046B1 (de) 1992-03-25
ATE74209T1 (de) 1992-04-15
EP0228046A2 (de) 1987-07-08
DK612186A (da) 1987-06-21
FI865166A (fi) 1987-06-21

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