ES2028779T3 - Promotor de levadura y procedimiento para preparar proteina heterologa. - Google Patents

Promotor de levadura y procedimiento para preparar proteina heterologa.

Info

Publication number
ES2028779T3
ES2028779T3 ES198686113675T ES86113675T ES2028779T3 ES 2028779 T3 ES2028779 T3 ES 2028779T3 ES 198686113675 T ES198686113675 T ES 198686113675T ES 86113675 T ES86113675 T ES 86113675T ES 2028779 T3 ES2028779 T3 ES 2028779T3
Authority
ES
Spain
Prior art keywords
preparing
procedure
promoter
protein
gap
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686113675T
Other languages
English (en)
Inventor
Hiromichi Mukai
Hajime Horii
Muneo Tsujikawa
Haruhide Kawabe
Hirofumi Arimura
Tadakazu Suyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GREEN CROSS Corp
Original Assignee
GREEN CROSS Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP61021198A external-priority patent/JPH0655155B2/ja
Application filed by GREEN CROSS Corp filed Critical GREEN CROSS Corp
Application granted granted Critical
Publication of ES2028779T3 publication Critical patent/ES2028779T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

SE PRESENTA UNA SECUENCIA DE ADN PROMOTORA DE LA GLICERALDEHIDO -3FOSFATO DESHIDROGENASA (GAP-DH), Y UN PROCEDIMIENTO PARA PREPARAR UNA PROTEINA HETEROLOGA UTILIZANDO DICHA SECUENCIA DE ADN COMO PROMOTOR, COMPRENDIENDO DICHO PROMOTOR DE GAP-DH UNA REGION SUPERIOR DE UN CODON INICIADOR DE LA PROTEINA GTAP-DH HASTA -164 BP COMO UNIDAD MINIMA. COMO EL PROMOTOR DE GAP-DH ES DE PEQUEÑO TAMAÑO, UNA SUSTANCIA FISIOLOGICAMENTE ACTIVA PUEDE SER EXPRESADA EFICAZMENTE EN LEVADURAS Y LA RECOMBINACION DEL ADN PUEDE SIMPLIFICARSE.
ES198686113675T 1985-10-03 1986-10-03 Promotor de levadura y procedimiento para preparar proteina heterologa. Expired - Lifetime ES2028779T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP21919185 1985-10-03
JP61021198A JPH0655155B2 (ja) 1985-10-03 1986-02-04 蛋白質の製造方法

Publications (1)

Publication Number Publication Date
ES2028779T3 true ES2028779T3 (es) 1992-07-16

Family

ID=26358233

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686113675T Expired - Lifetime ES2028779T3 (es) 1985-10-03 1986-10-03 Promotor de levadura y procedimiento para preparar proteina heterologa.

Country Status (4)

Country Link
US (1) US5021339A (es)
EP (1) EP0218209B1 (es)
DE (1) DE3683821D1 (es)
ES (1) ES2028779T3 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03155791A (ja) * 1989-11-14 1991-07-03 Green Cross Corp:The 雑種プロモーター

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341302C (en) * 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
US4618578A (en) * 1984-07-17 1986-10-21 Chiron Corporation Expression of glycoprotein D of herpes simplex virus
EP0164556B1 (en) * 1984-05-11 1994-03-02 Chiron Corporation Enhanced yeast transcription employing hybrid promoter region constructs
US4722840A (en) * 1984-09-12 1988-02-02 Chiron Corporation Hybrid particle immunogens

Also Published As

Publication number Publication date
EP0218209A2 (en) 1987-04-15
US5021339A (en) 1991-06-04
EP0218209B1 (en) 1992-02-05
EP0218209A3 (en) 1988-08-31
DE3683821D1 (de) 1992-03-19

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