ES2006560A6 - Procedimiento y equipo para la rapida determinacion del calculo de espermatozoides yno espermatozoides vivos. - Google Patents

Procedimiento y equipo para la rapida determinacion del calculo de espermatozoides yno espermatozoides vivos.

Info

Publication number
ES2006560A6
ES2006560A6 ES888800385A ES880385A ES2006560A6 ES 2006560 A6 ES2006560 A6 ES 2006560A6 ES 888800385 A ES888800385 A ES 888800385A ES 880385 A ES880385 A ES 880385A ES 2006560 A6 ES2006560 A6 ES 2006560A6
Authority
ES
Spain
Prior art keywords
intensity
sperm
buffer
count
living
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES888800385A
Other languages
English (en)
Inventor
Istvan Resli
Tibor Takacs
Sandor Damjanovich
Rezso Gaspar
Lajos Tron
Janos Szollosi
Janos Matko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Innofinance Altalanos Innovacios Penzintezet
Original Assignee
Innofinance Altalanos Innovacios Penzintezet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innofinance Altalanos Innovacios Penzintezet filed Critical Innofinance Altalanos Innovacios Penzintezet
Publication of ES2006560A6 publication Critical patent/ES2006560A6/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO Y EQUIPO PARA LA RAPIDA DETERMINACION DEL CALCULO DE ESPERMATOZOIDES Y/O DE ESPERMATOZOIDES VIVOS. EL PROCEDIMIENTO COMPRENDE LA DISOLUCION DEL YODURO DE PROPIDIO DEL COLORANTE FLUORESCENTE EN UN NEUTRALIZADOR Y LA MEDICION DE LA INTENSIDAD (F0), LA ULTERIOR ADICION DE LA MUESTRA DE ESPERMA, Y TRAS LA MEDICION DE LA INTENSIDAD (F1) Y LA ADICION DE UN AGENTE PERMEABILIZANTE DE LAS MEMBRANAS DEL CITOPLASMA, LA DETERMINACION DE LA INTENSIDAD (F2), LA ADICION DE UN NEUTRALIZADOR Y DE UN AGENTE PERMEABILIZANTE AL NEUTRALIZADOR Y LA MEDICION DE LA INTENSIDAD (F3) Y SUBSIGUIENTEMENTE DE LA INTENSIDAD (F4) DEL NEUTRALIZADOR PURO Y FINALMENTE DE LA INTENSIDAD (F5) DE LA MEZCLA DE ESPERMA A/ADIDA AL NEUTRALIZADOR, Y EL CALCULO DE LA CONCENTRACION DE CELULAS MEDIANTE USO DE LA FORMULA: PREDILUCION.
ES888800385A 1988-01-28 1988-02-12 Procedimiento y equipo para la rapida determinacion del calculo de espermatozoides yno espermatozoides vivos. Expired ES2006560A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8801870A GB2214518A (en) 1988-01-28 1988-01-28 Process and equipment for the rapid determination of the spermium cell count and/or living spermium count

Publications (1)

Publication Number Publication Date
ES2006560A6 true ES2006560A6 (es) 1989-05-01

Family

ID=10630656

Family Applications (1)

Application Number Title Priority Date Filing Date
ES888800385A Expired ES2006560A6 (es) 1988-01-28 1988-02-12 Procedimiento y equipo para la rapida determinacion del calculo de espermatozoides yno espermatozoides vivos.

Country Status (10)

Country Link
US (1) US4880732A (es)
JP (1) JPH01210863A (es)
AU (1) AU596270B2 (es)
CH (1) CH675485A5 (es)
DE (1) DE3806556A1 (es)
ES (1) ES2006560A6 (es)
FR (1) FR2626979B1 (es)
GB (1) GB2214518A (es)
NL (1) NL8800239A (es)
SE (1) SE460121B (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2044778A1 (es) * 1992-04-02 1994-01-01 Esteve Carmen Blanco Metodo analitico para la determinacion de la capacidad fecundante de una muestra de semen humano.

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2561170B2 (ja) * 1990-07-13 1996-12-04 剛 野間 リンパ球の抗原特異的インターロイキンー２反応性の測定法
US5935800A (en) * 1994-10-31 1999-08-10 Beth Israel Deaconess Medical Center Assays and kits for determining male fertility
RU2130183C1 (ru) * 1997-02-06 1999-05-10 Габбасов Зуфар Ахнафович Измеритель количества сперматозоидов или эритроцитов, движущихся в заданном диапазоне скоростей
CN1219048C (zh) * 1998-02-19 2005-09-14 医疗电子系统有限公司 精子分析系统
WO2000054026A1 (en) * 1999-03-05 2000-09-14 Hatting-Ks Determination of sperm concentration and viability for artificial insemination
US6359683B1 (en) * 2000-04-27 2002-03-19 Becton, Dickinson And Company Method for determining the volume of particles suspended in liquids
KR100834588B1 (ko) * 2001-05-24 2008-06-02 엠.이.에스. 메디컬 일렉트로닉 시스템즈 엘.티.디. 정액 분석
TW201109654A (en) * 2009-09-14 2011-03-16 Univ Nat Taiwan Biochip system, method for determining sperm quality and method for isolating sperm

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4146604A (en) * 1973-05-31 1979-03-27 Block Engineering, Inc. Differential counting of leukocytes and other cells
US4559309A (en) * 1982-09-01 1985-12-17 Memorial Sloan Kettering Cancer Center Flow cytometry-fluorescence measurements for characterizing sperm
GB2145112B (en) * 1983-04-27 1987-02-18 Milk Marketing Board Sorting living spermatozoa
NZ207393A (en) * 1983-08-05 1987-03-31 Neal Lloyd First Staining dna in living cells
US4693972A (en) * 1984-01-16 1987-09-15 Becton, Dickinson And Company Composition and method for rapid detection of microorganisms in clinical samples
US4683213A (en) * 1985-04-22 1987-07-28 Wisconsin Alumni Research Foundation Method for predicting the fertility of male mammals

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2044778A1 (es) * 1992-04-02 1994-01-01 Esteve Carmen Blanco Metodo analitico para la determinacion de la capacidad fecundante de una muestra de semen humano.

Also Published As

Publication number Publication date
SE8800325D0 (sv) 1988-02-02
US4880732A (en) 1989-11-14
NL8800239A (nl) 1989-09-01
SE8800325L (sv) 1989-08-03
AU1118588A (en) 1989-08-03
CH675485A5 (es) 1990-09-28
DE3806556A1 (de) 1989-09-07
FR2626979A1 (fr) 1989-08-11
AU596270B2 (en) 1990-04-26
JPH01210863A (ja) 1989-08-24
GB2214518A (en) 1989-09-06
SE460121B (sv) 1989-09-11
GB8801870D0 (en) 1988-02-24
FR2626979B1 (fr) 1990-07-20

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19990405