Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/46—Hybrid immunoglobulins
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
  • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
  • C07K16/2809—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
  • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
  • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/52—Constant or Fc region; Isotype
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/55—Fab or Fab'
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
  • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components

Definitions

• the present invention relates to novel covalent multi-specific antibodies and uses thereof.
• a bispecific antibody is an artificial antibody capable of binding to at least two antigens. By simultaneous engagement of at least two targets of interest, the bi-specific antibody can deliver benefits superior to conventional monospecific antibodies via novel and unique mechanisms.
• Blinatumomab CD3 ⁇ CD19, Amgen
• CD3 ⁇ CD19, Amgen targets CD3 and CD19 can efficiently engage T cells in the killing of CD19-expressing tumor cells via its CD3-regconizing Fv and showed superior efficacy over conventional antibodies in treating ALL (acute lymphoid leukemia) etc.
• ALL acute lymphoid leukemia
• BiTE Bi-specific T-cell Engaging (Micromet, acquired by Amgen in 2012) , CrossMab (Roche) , DVD-Ig (Abbvie) , TandAb (Affimed) , and DART (Dual Antigen Re-Targeting, Macrogenics) .
• bi-specific or multi-specific antibodies also introduce challenges, for example, in manufacture. Mispairing can happen between heavy chains and/or between heavy chain and light chain. How to efficiently and effectively remove the mispaired by-products has been an insurmountable challenge for bispecific or multi-specific antibodies. Therefore, there exists great needs to develop novel constructs that can provide for both good binding affinity to targets of interest but are also convenient for manufacture and downstream purification.
• an antibody means one antibody or more than one antibody.
• the present disclosure provides, among others, novel fusion polypeptides, nucleotide sequences encoding such, and the uses thereof.
• the present disclosure provides a fusion polypeptide comprising from C terminus to N terminus: a) a first target-binding fragment A1; b) a polypeptide linker; c) a second target-binding fragment B2, wherein the polypeptide linker has a length that is sufficiently short to minimize potential intramolecular interaction between A1 and B2.
• the A1 is capable of pairing with a first pairing fragment B1, to form a first target binding domain;
• the B2 is capable of pairing with a second pairing fragment A2, to form a second target binding domain.
• the A1 is configured to exhibit less binding to the B2 relative to the B1, and the B2 is configured to exhibit less binding to the A1 relative to the A2.
• the present disclosure provides a polypeptide complex comprising: a) the fusion polypeptide provided herein; b) a second polypeptide comprising the first pairing fragment B1; c) a third polypeptide comprising the second target binding fragment B2; and d) a fourth polypeptide and the fifth polypeptide each comprising the second pairing fragment A2, wherein the A1 in the fusion polypeptide pairs with the B1 in the second polypeptide to form a first target binding domain; the B2 in the fusion polypeptide pairs with the A2 in the fourth polypeptide to form a second target binding domain; and the A2 in the fifth polypeptide pairs with the B2 in the third polypeptide to form another second target binding domain.
• At least one of the pair of B1 and A1 and the pair of B2 and A2 contains at least one configuration capable of discouraging mispairing between B1 and A2 and/or between B2 and A1.
• the A1 comprises a first antibody variable region VA1 selected from VH1 or VL1.
• the B1 comprises a first pairing antibody variable region VB1 capable of pairing with VA1 to form the first target-binding domain, wherein the VB1 is selected from VH1 or VL1.
• the B2 comprises a second antibody variable region VB2 selected from VH2 or VL2.
• the A2 comprises a second pairing antibody variable region VA2 capable of pairing with VB2 to form the second target-binding domain, wherein the VA2 is selected from VH2 or VL2.
• the VB1 comprises VH1
• the VA1 comprises VL1
• the VB2 comprises VH2
• the VA2 comprises VL2.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VL2, and the VA2 comprises VH2. In some other embodiments, the VB1 comprises VL1, the VA1 comprises VH1, the VB2 comprises VH2, and the VA2 comprises VL2.
• the A1 further comprises a first scaffold region SR a operably linked to the VA1
• the B2 further comprises a second scaffold region SR b operably linked to the VB2, wherein the SR a and the SR b are configured such that pairing between the SR a and the SR b is discouraged.
• the B1 further comprises a first pairing scaffold region PSR a that is operably linked to the VB1 and is capable of binding to the SR a
• the A2 further comprises a second pairing scaffold region PSR b that is operably linked to the VA2 and is capable of binding to the SR b .
• the pair of SR a /PSR a or the pair of SR b /PSR b is/are selected from the group consisting of: a) a pair of heavy chain constant region 1 (CH1) and light chain constant region (CL) ; b) a pair of T cell receptor (TCR) constant region alpha (C alpha ) and TCR constant region beta (C beta ) ; c) a pair of TCR constant region gamma (C gamma ) and TCR constant region delta (C delta ) ; d) a pair of ligand-binding domain of a receptor and the ligand; and e) a pair of PRD (proline rich domain) and SH3 domain; f) a pair of obscurin and titin.
• CH1 heavy chain constant region 1
• CL light chain constant region
• the pair of the SR a and the PSR a is distinct from the pair of the SR b and the PSR b .
• the pair of the SR a and the PSR a is a pair of CH1 and CL
• the pair of the SR b and the PSR b is a pair of i) C alpha and C beta , ii) C gamma and C delta , iii) ligand-binding domain of a receptor and the ligand, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of CH1 and CL
• the pair of the SR a and the PSR a is a pair of i) C alpha and C beta , ii) C gamma and C delta , iii) ligand-binding domain of a receptor and the ligand, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• both the pair of SR a /PSR a and the pair of SR b /PSR b are the same, and the pair of SR a /PSR a and/or the pair of SR b /PSR b are configured such that mispairing between SR a /PSR b or between SR b /PSR a is discouraged.
• the pair of SR a /PSR a comprises a CH1 domain CH1a and a CL domain CLa
• the pair of SR b /PSR b comprises a CH1 domain CH1b and a CL domain CLb.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VH2, and the VA2 comprises VL2.
• one pair of the CH1 domain and the CL domain is crossed.
• the PSR a is CL domain CLa
• the SR a is CH1 domain CH1a
• the SR b is CH1 domain CH1b
• the PSR b is CL domain CLb.
• the PSR a is CH1 domain CH1a
• the SR a is CL domain CLa
• the SR b is CL domain CLb
• the PSR b is CH1 domain CH1b.
• the PSR a is CH1 domain CH1a
• the SR a is CL domain CLa
• the SR b is CH1 domain CH1b
• the PSR b is CL domain CLb.
• one pair of the VH domain and the VL domain is crossed.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VL2, and the VA2 comprises VH2.
• the VB1 comprises VL1, the VA1 comprises VH1, the VB2 comprises VH2, and the VA2 comprises VL2.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VH2, and the VA2 comprises VL2, the PSR a is CH1 domain CH1a, the SR a is CL domain CLa, the SR b is CH1 domain CH1b, the PSR b is CL domain CLb, and wherein: a) the fusion polypeptide comprises an amino acid sequence of Formula (I) : VH2-CH1b-Linker-VL1-CLa; b) the second polypeptide comprises an amino acid sequence of Formula (II) : VH1-CH1a; c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1b; d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CLb; and wherein the pair of CH1b/CLb and/or the pair of CH1a/CLa are configured such that mispairing between CH1b and CLa and/or between
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa have one or more characteristics shown below: 1) having at least one non-natural disulfide bond that discourages mispairing between CH1b and CLa and/or between CH1a and CLb; 2) having at least one or more introduced charged amino acid residues that discourages mispairing between CH1b and CLa and/or between CH1a and CLb; or 3) having one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that discourages mispairing between CH1b and CLa or between CH1a and CLb. These characteristics are useful for discouraging mispairing between CH1b and CLa and/or between CH1a and CLb.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa has at least one non-natural disulfide bond that discourages mispairing between CH1b and CLa and/or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected from CH1b/CLb and CH1a/CLa, and wherein the first CH1/CL pair is associated by a first disulfide bond that is non-natural, and optionally lacks a natural disulfide bond or has a disrupted natural disulfide bond.
• the second CH1/CL pair is associated by a second disulfide bond which is formed at a position different from the first disulfide bond, and optionally the second disulfide bond is a natural disulfide bond.
• the first disulfide bond is formed by two cysteine residues introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 126 –light chain EU position 121, b) heavy chain EU position 173 –light chain EU position 160, and c) heavy chain EU position 128 –light chain EU position 118.
• the natural disulfide bond is between heavy chain EU position 220 and light chain EU position 214.
• the first CH1/CL pair comprises a CH1 comprising substitution at EU position 126 for a cysteine residue, and substitution at EU position 220 for a non-cysteine residue; and a CL comprising substitution at EU position 121 for a cysteine residue, and substitution at EU position 214 for a non-cysteine residue.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa has at least one or more introduced charged amino acid residues that discourages mispairing between CH1b and CLa and/or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected from CH1b/CLb and CH1a/CLa, and wherein the first CH1/CL pair contains at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the first CH1/CL pair contains a first pair of oppositely charged residues that favors pairing of the first CH1/CL pair.
• the second CH1/CL pair contains at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the second CH1/CL pair contains a second pair of oppositely charged residues that favors pairing of the second CH1/CL pair, and optionally the first pair of oppositely charged residues and the second pair of oppositely charged residues discourages pairing of CH1a with CLb or CH1b and CLa.
• the first pair of oppositely charged residues and/or the second pair of oppositely charged residues are configured such that CH1a and CLb have both positive or both negative charged residues, and/or CH1b and CLa have both positive or both negative charged residues.
• first pair of oppositely charged residues and/or the second pair of oppositely charged residues is introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 183: light chain EU position 176, b) heavy chain EU position 183: light chain EU position 133, c) heavy chain EU position 147: light chain EU position 176, d) heavy chain EU position 141: light chain EU position 116, e) heavy chain EU position 126: light chain EU position 121, and f) heavy chain EU position 218: light chain EU position 122.
• the pair of oppositely charged residues comprises a positive-charged amino acid residue and a negative-charged amino acid residue
• the positive-charged amino acid residue is selected from the group consisting of lysine (K) , histidine (H) and arginine (R)
• the negative-charged amino acid residue is selected from the group consisting of aspartic acid (D) and glutamic acid (E) .
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa has one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that discourages mispairing between CH1b and CLa or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected respectively from CH1b/CLb and CH1a/CLa, and wherein the first CH1/CL pair comprises one or more introduced amino acid mutations that form an orthogonal CH1-CL interface.
• the orthogonal CH1-CL interface introduced at a set of heavy chain-light chain EU positions including heavy chain EU positions H168A, F170G, and light chain EU positions L135Y, S176W.
• the first CH1/CL pair comprises one or more introduced amino acid mutations that form orthogonal Fab designs at a set of heavy chain-light chain EU positions selected from the group consisting of: a) substitutions at heavy chain EU positions A141I, F170S, S181M, S183A, and V185A, and substitutions at light chain EU positions F116A, A235V, S174A, S176F, and T178V.
• the pair of VH1/VL1 and/or the pair of VH2/VL2 are configured such that mispairing between VH1 and VL2 and/or between VH2 and VL1 is discouraged.
• a first VH/VL pair and a second VH/VL pair are selected from VH1/VL1 and VH2/VL2, and wherein the first VH/VL pair has at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the first VH/VL contains a third pair of oppositely charged residues that favors pairing of first VH/VL pair.
• the second VH/VL pair has at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the second VH/VL contains a fourth pair of oppositely charged residues that favors pairing of second VH/VL pair, and optionally the third pair of oppositely charged residues and the fourth pair of oppositely charged residues discourages pairing of VH1 with VL2 or VH2 with VL1.
• the third pair of oppositely charged residues and the fourth pair of oppositely charged residues are configured such that VH1 and VL2 have both positive or both negative charged residues, and/or VH2 and VL1 have both positive or both negative charged residues.
• the third pair of oppositely charged residues and/or the fourth pair of oppositely charged residues is introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 39: light chain EU position 38; b) heavy chain EU position 105: light chain EU position 43, and c) heavy chain EU position 62: light chain EU position 1, or any combination thereof.
• the pair of oppositely charged residues comprises a positive-charged amino acid residue and a negative-charged amino acid residue
• the positive-charged amino acid residue is selected from the group consisting of lysine (K) , histidine (H) and arginine (R)
• the negative-charged amino acid residue is selected from the group consisting of aspartic acid (D) and glutamic acid (E) .
• the second polypeptide and the third polypeptide further comprise an operably linked first dimerization domain and an operably linked second dimerization domain, respectively, that are associated to form a dimer; optionally, the first dimerization domain comprises a first Fc region, and/or the second dimerization domain comprises a second Fc region.
• the first Fc region and/or the second Fc region of the polypeptide complex is derived from IgG1, IgG2, IgG3 or IgG4.
• the first Fc region and the second Fc region of the polypeptide complex have different amino acid sequences and have at least one configuration that facilitates heterodimerization of the first Fc region and the second Fc region.
• the first Fc region of the polypeptide complex comprises a first Fc mutation
• the second Fc region of the polypeptide complex comprises a second Fc mutation
• the first Fc mutations comprises T366W or S354C
• the second Fc mutation comprises Y349C, T366S, L368A, or Y407V
• the first Fc mutation comprises D399K or E356K
• the second Fc mutation comprises K392D, or K409D
• the first Fc mutation comprises E356K, E357K, or D399K
• the second Fc mutation comprises K370E, K409D, or K439E
• the first Fc mutation comprises S364H, or F405A
• the second Fc mutation comprises Y349T, or T394F
• the first Fc mutation comprises S364H, or T394F
• the second Fc mutation comprises Y394T
• At least one of the first target binding domain and the second target binding domain is chimeric, humanized, or fully human.
• the first target binding domain and the second target binding domain bind to different targets. In some embodiments, at least one of the first target binding domain and the second target binding domain binds to a tumor-associated antigen, or an immune-related target. In some embodiments, one of the first target binding domain and the second target binding domain binds to a tumor-associated antigen, and the other binds to an immune-related target.
• the disease-associated antigen is a tumor-associated antigen, an antigen associated with an autoimmune diseases or an inflammatory disease, or an antigen associated with an eye disorder, an antigen associated with central nervous system disease, an antigen associated with an infectious disease or an antigen associated with a coagulation disease.
• nucleic acid comprising a nucleotide sequence encoding the fusion polypeptide or the polypeptide complex as described herein.
• the present disclosure provides a vector comprising the nucleic acid described herein.
• the present disclosure provides a host cell comprising the nucleic acid and the vector described herein.
• the present disclosure provides a pharmaceutical composition comprising the polypeptide complex described herein and a pharmaceutically acceptable carrier.
• the present disclosure provides a conjugate comprises the polypeptide complex described herein, and a payload conjugated thereto, wherein the payload is selected from the group consisting of a radioactive label, a fluorescent label, an enzyme-substrate label, an affinity purification tag, a tracer molecule, an anticancer drug, an immune-related molecule, and a cytotoxic molecule.
• the present disclosure provides a composition comprising the polypeptide complex or the conjugate described herein, and a pharmaceutically acceptable carrier.
• the present disclosure provides a method of treating or preventing from a disease, condition, or symptom.
• the method comprises administering to a subject in need thereof a therapeutically effective amount of the polypeptide complex described herein, the pharmaceutical composition described herein, the conjugate described herein, or the composition described herein.
• the disease is selected from the group consisting of a cancer, an inflammatory disease, an infectious or parasitic disease, a cardiovascular disease, eye disease, central nerves system (CNS) disease, an injury, metabolic disease, autoimmune disease, or a coagulation disorder.
• the CNS disease is neuropathy, a neuropsychiatric condition, neuroblastoma, glioblastoma, or Alzheimer Disease.
• the present disclosure provides a method of detecting presence or level of an antigen.
• the method comprises contacting a sample suspected of containing the antigen with the polypeptide complex described herein and determining the formation of a complex between the antigen and the polypeptide complex.
• Figure 1 depicts each polypeptide fragment of an exemplary polypeptide complex which is constructed by two pairs of monoclonal antibodies and a pair of Fc regions.
• Figure 2 depicts diagrammatically the structure of an exemplary polypeptide complex constructed by the method of the present invention (Method B) .
• Figure 3 further depicts the structure of an exemplary polypeptide complex constructed by the method of the present invention (Method B) with the Fab fragments from both monoclonal antibodies subdivided into VH/VL and CH1/CL pairs.
• Figures 4A and 4B further depict the structure of an exemplary polypeptide complex constructed by the method of the present invention (Method B) with defined VH/VL and CH1/CL pairs of Fab fragments from both monoclonal antibodies.
• Figures 4C-4E depict the antibody structures with B1-A2 mispairing (Figure 4C) , B2-A1 mispairing ( Figure 4D) , and B1-A2 and B2-A1 mispairing ( Figure 4E) , respectively.
• Figures 5A and 5B depict the diagram of the structure of an exemplary bispecific antibody constructed by the conventional approach (Method A) .
• Figures 5C-5E depict the antibody structures with B1-A2 mispairing (Figure 5C) , B2-A1 mispairing ( Figure 5D) , and B1-A2 and B2-A1 mispairing ( Figure 5E) , respectively.
• Figures 6A and 6B indicate the expression results of exemplary polypeptide complexes (FORMAT EX1-8 and FORMAT NEW1-8) analyzed by SDS-Page.
• Figures 7A-7P indicate the expression results of exemplary polypeptide complexes (FORMAT EX1-8 and FORMAT NEW1-8) analyzed by SEC-HPLC.
• Figure 8 depicts the purity of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) analyzed by SDS-Page.
• Figure 9 depicts the purity of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) analyzed by CE-SDS.
• Figure 10 depicts the purity of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) analyzed by SEC-HPLC.
• Figure 11 depicts the purification profile of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) conducted by Affinity Chromatography.
• Figure 12 shows the SEC-HPLC purity analysis of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) purified by Affinity Chromatography.
• Figure 13 shows the SDS-Page purity analysis of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) purified by Affinity Chromatography.
• Figure 14 depicts the purification profile of the expressed polypeptide complex anti-CD20 x CD3 (FORMAT NEW7) under linear gradient elution by CEX.
• Figure 15 shows the SDS-Page purity analysis of the expressed polypeptide complex anti-CD20 x CD3 (fractions C01-C04) purified by the CEX.
• Figure 16 shows the SEC-HPLC purity analysis of the expressed polypeptide complex anti-CD20 x CD3 (fractions C01-C04) purified by the CEX.
• Figure 17 shows the sequences of exemplary scaffold region pairs used in the bispecific antibodies.
• a polypeptide complex means one polypeptide complex or more than one polypeptide complex.
• the terms “about, ” “approximately, ” “around” or alike refer to a quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by as much as 30, 25, 20, 25, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1%to a reference quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length.
• the terms “about” or “approximately” when preceding a numerical value indicates the value plus or minus a range of 15%, 10%, 5%, or 1%.
• the terms “comprise, ” “comprises, ” “comprising, ” “include, ” “includes, ” “including, ” “contain, ” “contains, ” “containing” , “have, ” “has, ” “having” and the like, are synonymous and are used inclusively, in an open-ended fashion, and do not exclude additional elements, features, steps, acts, operations, and so forth.
• the term “or” is used in its inclusive sense (and not in its exclusive sense) so that when used, for example, to connect a list of elements, the term “or” means one, some, or all of the elements in the list.
• the phrase “at least one” means one or more, i.e. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more.
• a phrase referring to “at least one of” a list of items is construed to refer to any combination of those items, including single members.
• “at least one of: A, B, or C” is intended to cover: A, B, C, A and B, A and C, B and C, and A, B, and C.
• Conjunctive language such as the phrase “at least one of X, Y and Z, ” unless specifically stated otherwise, is otherwise understood with the context as used in general to convey that an item, term, etc. may be at least one of X, Y or Z. Thus, such conjunctive language is not generally intended to imply that certain embodiment requires at least one of X, at least one of Y, and at least one of Z to each be present.
• references “one embodiment, ” “an embodiment, ” “a particular embodiment, ” “a related embodiment, ” “a certain embodiment, ” “an additional embodiment, ” “some embodiments, ” “certain embodiments, ” or “a further embodiment” or combinations thereof, are to be understood to mean that a particular feature, structure or characteristic described in connection with this particular embodiment is included in at least one embodiment of the present disclosure.
• the presences or appearances of the foregoing phrases in various places throughout this disclosure are not necessarily all referring to the same embodiment.
• the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
• polypeptide , “peptide” , and “protein” are used interchangeably herein to designate a linear series of amino acid residues connected one to the other by peptide bonds, which includes proteins, polypeptides, oligopeptides, peptides, and fragments thereof.
• the protein may be made up of naturally occurring amino acids and/or synthetic (e.g., modified or non-naturally occurring) amino acids.
• polypeptide includes fusion proteins, including, but not limited to, fusion proteins with a heterologous amino acid sequence, fusions with heterologous and homologous leader sequences, with or without N-terminal methionine residues; immunologically tagged proteins; fusion proteins with detectable fusion partners, e.g., fusion proteins including as a fusion partner a fluorescent protein, ⁇ -galactosidase, luciferase, etc.; and the like.
• amino acid refers to a building block of a protein, a peptide, a polypeptide or an amino acid polymer, and the term “amino acid” further refers to a naturally occurring or synthetic amino acid, as well as any amino acid analog and amino acid mimetics that functions in a manner similar to the naturally occurring amino acid.
• Naturally occurring amino acids are those encoded by the genetic codes, as well as those amino acids that are later modified, e.g., hydroxyproline, gamma-carboxyglutamate, and O-phosphoserine.
• naturally occurring amino acids include the group of naturally occurring carboxy alpha-amino acids comprising alanine (three letter code: Ala, one letter code: A) , arginine (Arg, R) , asparagine (Asn, N) , aspartic acid (Asp, D) , cysteine (Cys, C) , glutamine (Gln, Q) , glutamic acid (Glu, E) , glycine (Gly, G) , histidine (His, H) , isoleucine (Ile, I) , leucine (Leu, L) , lysine (Lys, K) , methionine (Met, M) , phenylalanine (Phe, F) , proline (Pro, P) , serine (Ser, S) , threonine (Thr, T) , tryptophan (Trp, W) , tyrosine (Ty
• domain refers to a globular structure formed by one or more regions of one or more polypeptide chains comprising peptide loops (e.g., comprising 3 to 4 peptide loops) that are stabilized, for example, by ⁇ -pleated sheet and/or intrachain disulfide bond (s) .
• peptide loops e.g., comprising 3 to 4 peptide loops
• Fab domain see below for more details
• nucleic acid As used herein, the term “nucleic acid, ” “nucleic acid molecule, ” “nucleotide, ” “polynucleotide” or alike, is construed to refer to a nucleotide polymer of any length, can include both DNA and RNA, can be single-stranded or double-stranded, and includes analogs of naturally occurring polynucleotides in which one or more nucleotides are modified over naturally occurring nucleotides.
• antibody encompasses any immunoglobulin, monoclonal antibody, polyclonal antibody, chimeric antibody, humanized antibody, multispecific antibody, bispecific antibody, bivalent antibody or multivalent antibody that binds to a specific antigen.
• antibody encompasses any immunoglobulin, monoclonal antibody, polyclonal antibody, chimeric antibody, humanized antibody, multispecific antibody, bispecific antibody, bivalent antibody or multivalent antibody that binds to a specific antigen.
• IgA In mammals such as human, depending on the different types of heavy chains present in the immunoglobulins, there are five different classes/isotypes (i.e. IgA, IgD, IgE, IgG, and IgM, corresponding to the five Ig heavy chain types ⁇ , ⁇ , ⁇ , ⁇ , and ⁇ , respectively) of antibodies, which typically have different molecular and biological properties, functional locations, physiological functionalities, and pathological implications in diseases. Certain antibody classes may further include subclasses. For example, in human, IgA may include IgA1 and IgA2 subclasses, and IgG may include four subclasses denoted as IgG1, IgG2, IgG3, and IgG4, respectively.
• a mammalian antibody may exist as a monomer (e.g. IgD, IgE, and IgG) , a dimer (IgA) , a tetramer (IgM) , or a pentamer (IgM) .
• a monomer e.g. IgD, IgE, and IgG
• a dimer IgA
• a tetramer IgM
• pentamer IgM
• two types of light chain exist, including kappa ( ⁇ ) chain and lambda ( ⁇ ) chain.
• a native or naturally occurring antibody such as IgG generally includes two identical heavy (H) chains and two identical light (L) chains.
• Each light chain is linked to a heavy chain by one covalent disulfide bond or linkage formed between a pair of cysteine residues present respectively in the each light chain and the heavy chain, and the two heavy chains are further linked to one another through several disulfide bonds formed between cysteine residues in each heavy chain.
• the tetramer thus formed substantially takes a Y-like shape for the antibody, with the end of each fork arm containing an identical antigen-binding site (i.e. paratope) that interacts specifically with a corresponding epitope of the antigen.
• each heavy chain in a N-terminal-to-C-terminal direction, includes a variable region (VH, or HCVR) , followed by three or four constant regions ( “CHs” , with IgA, IgD, IgG containing three CH regions CH1, CH2 and CH3; and IgE and IgM containing four CH regions CH1, CH2, CH3 and CH4) , and each light chain includes a variable region (VL, or LCVR) and a constant region (CL) .
• VH variable region
• CHs constant regions
• each light chain includes a variable region (VL, or LCVR) and a constant region (CL) .
• VL region variable region aligns or associates with the variable region of its pairing heavy chain (i.e. the VH region) to together form an antigen-binding site for the antibody.
• variable region or “VR” as used herein means the region in heavy chain or light chain of an antibody that are responsible for antigen binding.
• the heavy chain variable region (VH or HCVR) contains three highly variable loops called “complementarity determining regions” (CDRs) , i.e., heavy (H) chain CDRs including HCDR1, HCDR2, HCDR3, and the light chain variable region (VL or LCVR) contains three light (L) chain CDRs including LCDR1, LCDR2, and LCDR3.
• CDR boundaries for antibodies may be defined or identified by the conventions of Kabat, Chothia, or Al-Lazikani (Al-Lazikani, B., Chothia, C., Lesk, A.
• each VH and VL comprises four FRs, and the CDRs and FRs are arranged from amino terminus to carboxyl terminus in the order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
• variable region does not necessarily need to include all of the three CDRs or all of the four FRs, and should be construed to encompass any variant or derivative of a native variable region from a native antibody, as long as such variant or derivative retains antigen-binding activity.
• constant region or “constant moiety” as used herein means the region in heavy chain or light chain of an antibody that are not directly involved in antigen binding. It should be understood that the term “constant region” or “constant moiety” as used herein does not necessarily need to include the full length of a native constant region of a native antibody, and should be construed to encompass any variant or derivative of such a native constant region or constant moiety, as long as such variant or derivative retains the ability to, for example, support stability of the antigen-binding domain, or retains the intended biological function such as effector functions such as secretion, transplacental mobility, Fc receptor binding, complement binding, and the like.
• CL region refers to the constant region of an immunoglobulin light chain that is adjacent to the VL region. CL region may span from about EU position 108 to about EU position 216 in an immunoglobulin light chain. In a native antibody, the constant region of each light chain (i.e. the CL region) associates with the first constant region of a pairing heavy chain (i.e. the CH1 region) .
• CH1 region encompasses the first (most amino terminal) constant region of an immunoglobulin heavy chain that extends from, about EU position 118 to at least about EU position 220 (e.g. can be extended to EU position 221, and so on) .
• the CH1 region is adjacent to the VH region, and is amino terminal to the hinge region of an immunoglobulin heavy chain molecule.
• hinge region in terms of an antibody includes the portion of a heavy chain molecule that joins the CH1 region to the CH2 region.
• the length of hinge region may vary depending on the defined boundaries of the CH1 region and of the CH2 region.
• the hinge region is normally flexible, thus allowing the two N-terminus antigen binding regions to move independently.
• CH2 region refers to the portion of a heavy chain immunoglobulin molecule that extends, e.g., from about EU position 231 to EU position 340.
• CH3 region refers to the portion of a heavy chain immunoglobulin molecule that extends approximately 110 residues from N-terminus of the CH2 domain, e.g., from about EU position 341 to EU position 445, 446 or 447.
• the CH3 domain typically forms the C-terminal portion of the antibody like IgG, IgA, and IgD.
• additional domains may extend from CH3 domain to form the C-terminal portion of the molecule (e.g. the CH4 domain in the ⁇ chain of IgM and the ⁇ chain of IgE) .
• Fc refers to a portion derived from an antibody, for example, IgG, mainly composed of the second (CH2) and third (CH3) constant regions of a first heavy chain bound to the CH2 and CH3 of a second heavy chain via one or more covalent bonds which are non-peptide bonds, for example, via disulfide bonding.
• the Fc portion of the antibody is responsible for various effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) , etc., but does not function in antigen binding.
• ADCC antibody-dependent cellular cytotoxicity
• CDC complement-dependent cytotoxicity
• an “antigen” or “Ag” as used herein refers to a compound, composition, peptide, polypeptide, protein or substance (e.g., polypeptide, carbohydrate, nucleic acid, lipid, or other naturally occurring or synthetic compound) that can be specifically recognized and bound by a component of the immune system, e.g., an antibody.
• the term “antigen” encompasses antigenic epitopes, e.g., fragments of an antigen which are antigenic epitopes.
• the term “antigen” and “target” are used interchangeably in the present disclosure.
• Fv with regard to an antibody refers to the smallest fragment of the antibody to bear the complete antigen-binding site.
• An Fv fragment consists of the variable domain of a single light chain bound to the variable domain of a single heavy chain.
• a number of Fv designs have been provided, including dsFvs, in which the association between the two domains is enhanced by an introduced disulfide bond; and scFvs can be formed using a peptide linker to bind the two domains together as a single polypeptide.
• Fvs constructs containing a variable domain of a heavy or light immunoglobulin chain associated to the variable and constant domain of the corresponding immunoglobulin heavy or light chain have also been produced.
• Fvs have also been multimerized to form diabodies and triabodies (Maynard et al., Annu Rev Biomed Eng 2 339-376 (2000) ) .
• Fab refers to a single antigen-binding domain derived from an antibody, where the domain has a single heavy chain fragment associated with a single light chain fragment via one or more covalent bonds which are non-peptide bonds.
• the single heavy chain fragment in the Fab domain comprises an HCVR and a CH1 region.
• the single light chain fragment in the Fab domain comprises an LCVR and a CL domain.
• the CH1 region associates with the HCVR by a covalent bond such as a disulfide bond.
• the Fab domain corresponds substantially to one arm of the antibody, typically retains the ability to recognize and bind to its corresponding antigen.
• multispecific antibody refers to an artificial or engineered antibody that can simultaneously bind to at least two different epitopes.
• a bispecific antibody is substantially a type of a multispecific antibody.
• other multispecific antibodies may include trispecific antibodies, which have three different antigen-binding specificities, tetraspecific antibodies, which have four different antigen-binding specificities, and so on.
• bispecific antibody refers to an antibody that comprises two physically separable antigen-binding domains which differ from one another in their antigen specificity.
• a bispecific antibody is an artificial antibody which has fragments derived from two different monoclonal antibodies and is capable of binding to two different epitopes. The two epitopes may present on the same antigen, or they may present on two different antigens. It is in contrast to a naturally occurring antibody which has two physically separable antigen-binding moieties that are structurally identical and thus have the same antigen specificity.
• the numbers indicating the positions of amino acid residues in a constant region of an antibody are based on the EU numbering, as described in Edelman, G. M. et al., Proc. Natl. Acad. USA, 63, 78-85 (1969) .
• some positions use IMGT numbering or Kabat index, as described in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md.
• chimeric means an antibody or antigen-binding fragment, having a portion of heavy and/or light chain derived from one species, and the rest of the heavy and/or light chain derived from a different species.
• a chimeric antibody may comprise a constant region derived from human and a variable region from a non-human animal, such as from mouse.
• the non-human animal is a mammal, for example, a mouse, a rat, a rabbit, a goat, a sheep, a guinea pig, or a hamster.
• valent refers to the presence of a specified number of antigen binding sites in a given molecule.
• monovalent refers to an antibody or an antigen-binding fragment having only one single antigen-binding site; and the term “multivalent” refers to an antibody or an antigen-binding fragment having multiple antigen-binding sites.
• bivalent denote the presence of two binding sites, four binding sites, and six binding sites, respectively, in an antigen-binding molecule.
• the antibody or antigen-binding fragment thereof is bivalent.
• Antibody and fragments thereof according to the present disclosure encompass bispecific and multi-specific antibodies and fragments thereof.
• Bispecific or multi-specific antibodies may resemble single antibodies (or antibody fragments) but have two or more different antigen binding sites.
• Bispecific antibodies may have binding specificities for at least two different epitopes.
• Bispecific antibodies and fragments can also be in form of heteroantibodies.
• Heteroantibodies are two or more antibodies, or antibody binding fragments (e.g., Fab) linked together, each antibody or fragment having a different specificity.
• telomere binding of an antibody or “antigen-specific antibody” in the context of a characteristic of an antibody refers to the ability of an antibody to preferentially bind to a particular antigen that is present in a mixture of different antigens.
• a specific binding interaction will discriminate between desirable and undesirable antigens (or “target” and “non-target” antigens) in a sample, in some embodiments more than about 10 to 100-fold or more (e.g., more than about 1000-or 10,000-fold) .
• the affinity between an antibody and antigen when they are specifically bound in an antibody antigen complex is characterized by a K D (dissociation constant) of less than 10 -6 M, less than 10 -7 M, less than 10 -8 M, less than 10 -9 M, less than 10 -9 M, less than 10 -11 M, or less than about 10 -12 M or less.
• K D dissociation constant
• vector refers to a vehicle into which a genetic element may be operably inserted so as to bring about the expression of that genetic element, such as to produce the protein, RNA or DNA encoded by the genetic element, or to replicate the genetic element.
• a vector may be used to transform, transduce, or transfect a host cell so as to bring about expression of the genetic element it carries within the host cell.
• vectors examples include plasmids, phagemids, cosmids, artificial chromosomes such as yeast artificial chromosome (YAC) , bacterial artificial chromosome (BAC) , or P1-derived artificial chromosome (PAC) , bacteriophages such as lambda phage or M13 phage, and animal viruses.
• a vector may contain a variety of elements for controlling expression, including promoter sequences, transcription initiation sequences, enhancer sequences, selectable elements, and reporter genes.
• the vector may contain an origin of replication.
• a vector may also include materials to aid in its entry into the cell, including but not limited to a viral particle, a liposome, or a protein coating.
• a vector can be an expression vector or a cloning vector.
• the present disclosure provides vectors (e.g., expression vectors) containing the nucleic acid sequence provided herein encoding the antibody or an antigen-binding fragment thereof, at least one promoter (e.g., SV40, CMV, EF-1 ⁇ ) operably linked to the nucleic acid sequence, and at least one selection marker.
• promoter e.g., SV40, CMV, EF-1 ⁇
• host cell refers to a cell into which an exogenous polynucleotide and/or a vector can be or has been introduced.
• Antibody conjugates are also provided.
• conjugates generally refer to a chemical linkage, either covalent or non-covalent that proximally associates one molecule with second molecule.
• the conjugates include any antibody of the present disclosure and an agent.
• the agent may be selected from a therapeutic agent, an imaging agent, a labeling agent, or an agent useful for therapeutic and/or labeling purposes.
• biological sample refers to a biological composition that is obtained or derived from a subject of interest that contains a cellular and/or other molecular entity that is to be characterized and/or identified, for example based on physical, biochemical, chemical and/or physiological characteristics.
• a biological sample includes, but is not limited to, cells, tissues, organs and/or biological fluids of a subject, obtained by any method known by those of skill in the art.
• the biological sample is a fluid sample.
• the fluid sample is whole blood, plasma, blood serum, mucus (including nasal drainage and phlegm) , peritoneal fluid, pleural fluid, chest fluid, saliva, urine, synovial fluid, cerebrospinal fluid (CSF) , thoracentesis fluid, abdominal fluid, ascites or pericardial fluid.
• the biological sample is a tissue or cell obtained from heart, liver, spleen, lung, kidney, skin or blood vessels of the subject.
• the present disclosure provides a novel multispecific (or bispecific) polypeptide complex that is convenient to manufacture and purify, therefore can be provided with high purity and high yields.
• Multispecific (or bispecific) antibodies are of great interest in the field of antibody engineering and have prospects of broad applications such as in anti-tumor immunotherapy.
• Different formats for bispecific antibodies have been developed, including IgG-like bispecific antibodies.
• IgG-like bispecific antibodies are bivalent, comprising two pairs of heavy chain (HC) and light chain (LC) derived from two different antibodies.
• An IgG-like bispecific antibody provides two monovalent Fabs, each binding to a different antigen. This, however, can lead to decrease in affinity to each antigen and even loss of certain functions, when compared with conventional IgG antibodies that provide bivalent binding to a single antigen.
• Another challenge relates to differentiating the binding affinity of an IgG-like bispecific antibody to the two target antigens.
• MOA mechanism of action
• an additional Fab domain targeting such a target can be fused to one of the heavy chains of the IgG-like bispecific antibody (see Figure 5A) .
• bispecific but trivalent antibody The structure of such a bispecific but trivalent antibody is referred to herein as 2: 1 structure, that is, the binding to one antigen of interest is bivalent, while the binding to the other antigen remains monovalent.
• bivalent binding restores binding affinity to that antigen target, and in the meantime can confer affinity difference between the two antigen targets.
• LC-HC binding instability and LC mispairing may occur during the assembling of such 2: 1 structure bispecific antibodies.
• the present disclosure provides a novel 2: 1 structure bispecific antibody platform that can not only meet the requirements for the affinity difference between two antigen targets but is also much easier to purify than conventional 2: 1 structure bispecific antibodies.
• This novel 2: 1 structure is advantageous because the potential mispaired products (if any) are sufficiently different from the correct-paired product, for example, in molecular weight as well as in physical and chemical characteristics, making it easier to remove the mispaired products. Therefore, the bispecific antibody constructed according to this novel 2: 1 structure platform can achieve higher purities and yields with relatively simple purification process.
• the novel 2: 1 structure provided herein is based on an IgG-like bispecific antibody, fused with an additional Fab domain binding to one of the two targets. Specifically, unlike conventional 2: 1 structure bispecific antibody in which the additional Fab domain is fused to a heavy chain of the Ig-like bispecific antibody (see, the second polypeptide chain in Figure 5A) , the novel 2: 1 structure provided herein has the additional Fab domain fused to a light chain of the IgG like bispecific antibody (see the first polypeptide chain in Figure 2) .
• the novel 2: 1 structure bispecific antibodies comprise a polypeptide complex composed of five polypeptide chains, including: 1) a first polypeptide chain comprising a fusion polypeptide comprising a first targeting binding fragment A1 and a second target binding fragment B2, 2) a second polypeptide comprising the first pairing fragment B1; 3) a third polypeptide comprising the second target binding fragment B2; 4) a fourth polypeptide comprising the second pairing fragment A2, and 5) a fifth polypeptide identical to the fourth polypeptide, i.e. also comprising the second target binding fragment A2.
• the uniqueness of the polypeptide complex provided herein at least lies in part in the fusion polypeptide (i.e. the first polypeptide chain) comprised therein, which is novel and not comprised in any of the conventional 2: 1 structure bispecific antibodies.
• This fusion polypeptide makes it possible that any mispaired products would have significantly different molecular weights as well as physical and chemical characteristics from the target bispecific product.
• the fusion polypeptide comprises from C terminus to N terminus: a first target-binding fragment A1; a polypeptide linker; a second target-binding fragment B2; wherein, the polypeptide linker has a length that is sufficiently short to minimize potential intramolecular interaction between A1 and B2, the A1 is capable of pairing with a first pairing fragment B1, to form a first target binding domain; the B2 is capable of pairing with a second pairing fragment A2, to form a second target binding domain; and the A1 is configured to exhibit less binding to the B2 relative to the B1, and the B2 is configured to exhibit less binding to the A1 relative to the A2.
• novel 2: 1 structure as shown in Figure 2 the two light chains are identical and therefore are useful to reduce unwanted mispairing.
• an illustrative novel 2: 1 structure provided herein (see Figure 4A) , a total of 3 different mispaired products (see Figures 4C-4E) are expected, which are estimated to have molecular weights of about 150 KDa, 250 KDa, and 200KDa, respectively (see Figure 4B) , and therefore most of the potential mispaired products have different molecular weights from the correct paired product.
• these potentially mispaired products also are shown to have different physical and chemical characteristics from the correct paired bispecific product, and therefore can be readily removed by conventional purification methods.
• the conventional 2: 1 structure bispecific antibody as shown in Figure 5A, has two different light chains that may potentially mispair with different heavy chains, rendering potentially a total of 5 different mispaired products (see Figures 5C-5E) , which are estimated to all have the same molecular weights (i.e. 200KDa) as the correct paired product (see Figure 5B) .
• These similar mispaired products are difficult to be separated from the target product, therefore compromising not only the purity but also the yield of the target product.
• the novel 2: 1 polypeptide complex provided herein binds to a first target and a second target. It has two antigen-binding domains that both bind to the second target, and a third antigen-binding domain that binds to the first target. In other words, it is bivalent for the second target and monovalent for the first target, and hence is referred to as “2: 1” .
• the two antigen binding domains for the second target are located on different arms of the polypeptide complex provided herein.
• the target-binding domains in the polypeptide complex provided herein are formed by pairing of two polypeptide fragments.
• the first target binding domain is formed by the pairing of the first target-binding fragment A1 and the first pairing fragment B1 (i.e. A1/B1 pair) .
• the second target binding domain is formed by the pairing of the second target-binding fragment A2 and the second pairing fragment B2 (i.e. A2/B2 pair) .
• the first target-binding fragment A1 and the first pairing fragment B1 can be paired by, for example, a disulfide bond, a hydrogen bond, electrostatic interaction, a salt bridge, or hydrophobic-hydrophilic interaction, a connecter, or the combination thereof, that is formed between A1 and B1, or more specifically, between at least two amino acid residues from A1 and B1.
• the second target-binding fragment B2 and the second pairing fragment A2 can be paired by, for example, a disulfide bond, a hydrogen bond, electrostatic interaction, a salt bridge, or hydrophobic- hydrophilic interaction, a connecter, or the combination thereof, that is formed between A2 and B2, or more specifically, between at least two amino acid residues from A2 and B2.
• a “disulfide bond” refers to a covalent bond with the structure R-S-S-R’.
• the amino acid cysteine comprises a thiol group that can form a disulfide bond with a second thiol group, for example from another cysteine residue.
• the disulfide bond can be formed between the thiol groups of two cysteine residues residing respectively on the two polypeptide chains, thereby forming an interchain disulfide bond.
• Disulfide bond can be formed between known scaffold polypeptide fragments, such as antibody CH1 domain and CL domain, or TCR constant regions (such as TCR alpha/TCR beta, TCR gamma/TCR delta) , to name just a few.
• Electrostatic interaction is non-covalent interaction and is important in protein folding, stability, flexibility and function, including ionic interactions, hydrogen bonding and halogen bonding. Electrostatic interactions can be formed in a polypeptide, for example, between Lys and Asp, between Lys and Glu, between Glu and Arg, or between Glu, Trp on the first polypeptide chain and Arg, Val or Thr on the second polypeptide chain.
• a salt bridge is close-range electrostatic interactions that mainly arises from the anionic carboxylate of either Asp or Glu and the cationic ammonium from Lys or the guanidinium of Arg, which are spatially proximal pairs of oppositely charged residues in native protein structures. Charged and polar residues in largely hydrophobic interfaces may act as hot spots for binding. Among others, residues with ionizable side chains such as His, Tyr, and Ser can also participate the formation of a salt bridge.
• a hydrophobic interaction can be formed between one or more Val, Tyr and Ala on one polypeptide chain and one or more Val, Leu, and Trp on the second chain, or His and Ala on the first chain and Thr and Phe on another polypeptide chain.
• a hydrogen bond is formed by electrostatic attraction between two polar groups when a hydrogen atom covalently bound to a highly electronegative atom such as nitrogen, oxygen, or fluorine.
• a hydrogen bond can be formed, for example, a nitrogen group in Asn and an oxygen group in His, or an oxygen group in Asn and a nitrogen group in Lys.
• the cognate pairing of A1 and B1 as shown in the novel 2: 1 structure of Figure 4A is facilitated by a native or an introduced non-native disulfide bond, whereas a mispaired product (such as mispaired A1 and B2, or mispaired A2 and B1) would lack such a disulfide bond.
• a mispaired product such as mispaired A1 and B2, or mispaired A2 and B1
• it is expected that such a disulfide bond would be particularly advantageous to permit easy identification and/or characterization of presence of mispaired products, for example, by using conventional methods such as sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) .
• the A1/B1 pair and/or the A2/B2 pair comprise an antibody Fv domain.
• the A1 comprises a first antibody variable region VA1 selected from VH1 or VL1
• the B1 comprises a first pairing antibody variable region VB1 capable of pairing with VA1 to form the first target-binding domain, wherein the VB1 is selected from VH1 or VL1.
• the first target-binding domain comprises a first Fv domain comprising VH1 and VL1 associated together that is capable of binding to the first target.
• the B2 comprises a second antibody variable region VB2 selected from VH2 or VL2
• the A2 comprises a second pairing antibody variable region VA2 capable of pairing with VB2 to form the second target-binding domain, wherein the VA2 is selected from VH2 or VL2.
• the second target-binding domain comprises a second Fv domain comprising VH2 and VL2 associated together that is capable of binding to the second target.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VH2, and the VA2 comprises VL2. In some embodiments, the VB1 comprises VL1, the VA1 comprises VH1, the VB2 comprises VL2, and the VA2 comprises VH2.
• the first Fv domain or the second Fv domain is crossed.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VL2, and the VA2 comprises VH2.
• the VB1 comprises VL1, the VA1 comprises VH1, the VB2 comprises VH2, and the VA2 comprises VL2.
• the Fv domains can be associated by any suitable means known in the art, for example without limitation, by a non-native disulfide bond introduced in the Fv domain, or by a non-native electrostatic interaction introduced in the Fv domain.
• the Fv domains can be associated by scaffold domains fused to the Fv domains.
• a scaffold region can be fused to one chain of the Fv domain, and a pairing scaffold region can be fused to the other chain of the Fv domain. Binding of the scaffold region to the pairing scaffold region can allow association of the VH region and the VL region, thereby forming the Fv domain that is capable of binding to the target antigen.
• the A1 further comprises a first scaffold region SR a operably linked to the VA1
• the B2 further comprises a second scaffold region SR b operably linked to the VB2, wherein the SR a and the SR b are configured such that pairing between the SR a and the SR b is discouraged.
• the B1 further comprises a first pairing scaffold region PSR a that is operably linked to the VB1 and is capable of binding to the SR a
• the A2 further comprises a second pairing scaffold region PSR b that is operably linked to the VA2 and is capable of binding to the SR b . Binding of SR a and PSR a forms the first scaffold domain, and binding of SR b and PSR b forms the second scaffold domain.
• An illustrative drawing is shown in Figure 3.
• the scaffold region can be selected from antibody CH1 domain and CL domain, pairing TCR constant regions (such as TCR alpha/TCR beta, TCR gamma/TCR delta) , or PRD (proline rich domain) and SH3 domain, or obscurin and titin.
• TCR constant regions such as TCR alpha/TCR beta, TCR gamma/TCR delta
• PRD proline rich domain
• SH3 domain or obscurin and titin.
• TCR belongs to the immunoglobulin superfamily, and is similar to a half antibody with a single heavy chain and a single light chain.
• Native TCR consists of two polypeptide chains, and has in general two types: one consists of alpha and beta chains (i.e. alpha/beta TCR) , and the other consists of gamma and delta chains (i.e. gamma/delta TCR) .
• the two TCR chains are linked by disulfide bonds formed between constant regions in the extracellular portion of the TCR chains. In other words, disulfide bonds are formed between TCR constant region alpha and TCR constant region beta, or between TCR constant region gamma and TCR constant region delta.
• the SH3 domain short for SRC Homology 3 Domain, is a small protein domain of about 60 amino acid residues, which was initially was described as a conserved sequence in the viral adaptor protein v-Crk.
• the classical SH3 domain is usually found in proteins that interact with other proteins and mediate assembly of specific protein complexes, typically via binding to proline-rich domains (i.e., PRD) in their respective binding partner (Liubov V Gushchina et al., J Biomol Struct Dyn. 2011 Dec; 29 (3) : 485-95. ) .
• Titin (also called connectin) is a protein that in humans is encoded by the TTN gene. Titin is a giant protein, greater than 1 ⁇ m in length, that functions as a molecular spring which is responsible for the passive elasticity of muscle. Obscurin ( ⁇ 800 kDa) , the newest member of the titin family, was initially identified as a ligand of a Zdisk portion of titin, although it primarily localizes to the sarcomeric M-band in mature muscle. The giant muscular proteins titin and obscurin bind to each other at the Zdisk during muscle development.
• a titin T-chain and an obscurin O-chain can be used to substitute the CH1 and CL regions of an antibody, wherein titin T-chain is a peptide containing titin Ig-like 152 domain with a length of 78-118 amino acids, or functional variants thereof, while obscurin O-chain is a peptide containing obscurin Ig-like 1 domain with a length of 87-117 amino acids, or functional variants thereof (see, e.g., WO2021/139757A, which is incorporated herein to its entirety) .
• Suitable pairs of scaffold regions useful in the present invention include: a) a pair of heavy chain constant region 1 (CH1) and light chain constant region (CL) ; b) a pair of T cell receptor (TCR) constant region alpha (Calpha) and TCR constant region beta (Cbeta) ; c) a pair of TCR constant region gamma (Cgamma) and TCR constant region delta (Cdelta) ; d) a pair of ligand-binding domain of a receptor and the ligand; e) a pair of PRD (proline rich domain) and SH3 domain; f) a pair of obscurin and titin, or the like.
• CH1 heavy chain constant region 1
• CL light chain constant region
• TCR T cell receptor
• Cbeta T cell receptor
• Cgamma TCR constant region beta
• Cdelta TCR constant region delta
• the expression “a pair of X and Y” or “the X/Y pair” does not intend to limit the sequential order.
• the pair of SR a /PSR a can be a pair of CH1 and CL”
• the SR a can be either CH1 or CL, and accordingly, when the SR a is CH1 then the PSR a can be CL, or when the SR a is CL then the PSR a can be CH1.
• the SR a and the PSR a are paired by a first disulfide bond. In such embodiments, mispairing of the SR a and the PSR b or the SR b and the PSR a would lack the first disulfide bond. In some embodiments, the SR b and the PSR b are paired by a second disulfide bond. In such embodiments, mispairing of the SR a and the PSR b or the SR b and the PSR a would lack the second disulfide bond. Any suitable pairs of scaffold regions capable of pairing by a disulfide bond can be used as the pair of SR a and the PSR a or the pair of SR b /PSR b .
• antibody CH1 domain and CL domain can be paired by a disulfide bond
• TCR constant regions such as Calpha and Cbeta, or Cgamma and Cdelta
• obscurin and titin can be paired by a disulfide bond.
• the pair of SR a /PSR a comprises a pair of a CH1 domain CH1a and a CL domain CLa
• the pair of SR b /PSR b comprises a pair of a CH1 domain CH1b and a CL domain CLb.
• An illustrative drawing is shown in Figure 4A.
• the first target binding domain (formed by A1/B1 pair) and the second target binding domain (formed by A2/B2 pair) comprise antibody Fab domains.
• the pairing between A1 and B1 can be formed between two amino acid residues in VA1 and VB1, in VH1 and VL1, in SR a and PSR a , or in CH1a and CLa.
• the pairing between A2 and B2 can be formed between two amino acid residues in VA2 and VB2, in VH2 and VL2, in SR b and PSR b , or in CH1b and CLb.
• a target-binding polypeptide fragment binds to its pairing polypeptide fragment to form a target-binding domain (i.e. cognate pairing)
• mispairing nevertheless may happen between polypeptide fragments that are not intended to pair.
• the first target-binding fragment A1 may mispair with the second target-binding fragment B2, or the first target-binding fragment A2 may mispair with the second target-binding fragment B1, thereby failing to form the intended target-binding domain.
• VH1 pairs with VL1 to form the first target-binding domain and VH2 pairs with VL2 to form the second target-binding domain, and such cognate pairing is intended to be facilitated.
• VH1 may mispair with VL2
• VH2 may mispair with VL1, and such mispairing is preferably discouraged or reduced.
• the polypeptide fragments can be designed or engineered to contain certain configurations that helps achieve the purpose.
• at least one of the pair of A1 and B1 (referred to as A1/B1) and the pair of A2 and B2 (referred to as A2/B2) contains at least one configuration capable of: a) facilitating cognate pairing between A1 and A2 and/or B1 and B2, and/or b) discouraging mispairing between B1 and A2 and/or between B2 and A1.
• any suitable methods known in the art can be used in the polypeptide complexes disclosed herein, to facilitate cognate pairing and/or reduce mispairing.
• Several strategies have been applied into designing orthogonal interfaces to facilitate cognate pairing. For example, Roche swapped the CH1 and CL domains and created the CrossMab platform (Schaefer et al. Proceedings of the National Academy of Sciences of the United States of America, 108 (27) , pp. 11187–11192 (2011) ) , MedImmune introduced non-native disulfide bond (Mazor et al. mAbs, 7 (2) , pp. 377–389 (2015) ) , Amgen further introduced electrostatic interactions in the CH1-CL region (Liu et al.
• both the pair of SR a /PSR a and the pair of SR b /PSR b are the same or of the same type, the pair of SR a /PSR a and/or the pair of SR b /PSR b are configured such that mispairing between SR a /PSR b or between SR b /PSR a is discouraged.
• the A1/B1 pair and the A2/B2 pair can be designed to incorporate crossed VA1/VB1 configuration (e.g. CrossMab) , or to incorporate distinct pairing scaffold regions, or to incorporate crossed pairing scaffold regions, or to introduce at least one mutation in the otherwise identical pairing scaffold regions. More details are discussed below.
• crossed VA1/VB1 configuration e.g. CrossMab
• the B1/A1 pair and the B2/A2 pair comprise antibody Fv domains and the Fv domains are crossed to discourage or reduce mispairing between B2 and A1, or between B1 and A2.
• Fv domains comprises a heavy chain variable domain VH and a light chain variable domain VL.
• the two Fv domains can be in crossed configuration such that when mispairing between B2 and A1 occurs and/or mispairing between B1 and A2 occurs, it would result in pairing of two VH domains or pairing of two VL domains, which are believed to be less stable than pairing between VH and VL, and hence less likely to form.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VL2, and the VA2 comprises VH2. In some embodiments, the VB1 comprises VL1, the VA1 comprises VH1, the VB2 comprises VH2, and the VA2 comprises VL2. In these cross VA/VB1 configurations, when B2 mispairs with A1, or when B1 mispairs with A2, it would result in VH1 pairing with VH2, or VL1 pairing with VL2, which are believed to be less likely to form stable products.
• the VB1 is operably linked to a first pairing scaffold region PSR a
• the VA1 is operably linked to the SR a
• the SR a binds to the PSR a to form the first pairing scaffold domain
• the VB2 is operably linked to a second pairing scaffold region SR b
• the VA2 is operably linked to the PSR b
• the SR b binds to the PSR b to form the second pairing scaffold domain.
• the PSR a is CH1 domain CH1a
• the SR a is CL domain CLa
• the SR b is CH1 domain CH1b
• the PSR b is CL domain CLb
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VL2, and the VA2 comprises VH2
• the VB1 comprises VL1
• the VA1 comprises VH1
• the VB2 comprises VH2
• the VA2 comprises VL2
• the B1/A1 pair comprises a first antibody Fv domain and a first pair of scaffold region (i.e. SR a /PSR a )
• the B2/A2 pair comprises a second antibody Fv domain and a second pair of scaffold region (i.e. SR b /PSR b )
• the first pair of scaffold region and the second scaffold regions are distinct, or are different types of scaffold regions. Accordingly, mispairing between A2 and B1, or mispairing between A1 and B2 would result in non-compatible scaffold regions that do not naturally bind to each other.
• the pair of the SR a and the PSR a is distinct from the pair of the SR b and the PSR b .
• the pair of SR a /PSR a or the pair of SR b /PSR b is selected from the group consisting of: a) a pair of heavy chain constant region 1 (CH1) and light chain constant region (CL) ; b) a pair of T cell receptor (TCR) constant region alpha (Calpha) and TCR constant region beta (Cbeta) ; c) a pair of TCR constant region gamma (Cgamma) and TCR constant region delta (Cdelta) ; d) a pair of ligand-binding domain of a receptor and the ligand; e) a pair of PRD (proline rich domain) and SH3 domain; and f) a pair of obscurin and titin.
• CH1 heavy chain constant region 1
• CL light chain constant region
• TCR T cell receptor
• the TCR constant region alpha (i.e., Calpha) comprises the amino acid sequence of SEQ ID NO: 97; and the TCR constant region beta (i.e., Cbeta) comprises the amino acid sequence of SEQ ID NO: 98.
• the pair of the SR a and the PSR a is a pair of CH1 and CL
• the pair of the SR b and the PSR b is a pair of: i) Calpha and Cbeta, ii) Cgamma and Cdelta, iii) ligand-binding domain of a receptor and the ligand; iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of CH1 and CL
• the pair of the SR a and the PSR a is a pair of: i) Calpha and Cbeta, ii) Cgamma and Cdelta, iii) ligand-binding domain of a receptor and the ligand, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR a and the PSR a is a pair of Calpha and Cbeta
• the pair of the SR b and the PSR b is a pair of: i) CH1 and CL, ii) Cgamma and Cdelta, iii) ligand-binding domain of a receptor and the ligand; iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of Calpha and Cbeta
• the pair of the SR a and the PSR a is a pair of: i) CH1 and CL, ii) Cgamma and Cdelta, iii) ligand-binding domain of a receptor and the ligand, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR a and the PSR a is a pair of Cgamma and Cdelta
• the pair of the SR b and the PSR b is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) ligand-binding domain of a receptor and the ligand; iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of Cgamma and Cdelta
• the pair of the SR a and the PSR a is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) ligand-binding domain of a receptor and the ligand, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR a and the PSR a is a pair of ligand-binding domain of a receptor and the ligand
• the pair of the SR b and the PSR b is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta; iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of ligand-binding domain of a receptor and the ligand
• the pair of the SR a and the PSR a is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta, iv) PRD (proline rich domain) and SH3 domain, or v) obscurin and titin.
• the pair of the SR a and the PSR a is a pair of PRD (proline rich domain) and SH3 domain
• the pair of the SR b and the PSR b is a pair of:i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta; iv) ligand-binding domain of a receptor and the ligand, or v) obscurin and titin.
• the pair of the SR b and the PSR b is a pair of PRD (proline rich domain) and SH3 domain
• the pair of the SR a and the PSR a is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta, iv) ligand-binding domain of a receptor and the ligand, or v) obscurin and titin.
• the pair of the SR a and the PSR a is a pair of obscurin and titin
• the pair of the SR b and the PSR b is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta; iv) ligand-binding domain of a receptor and the ligand, or v) PRD (proline rich domain) and SH3 domain.
• the pair of the SR b and the PSR b is a pair of obscurin and titin
• the pair of the SR a and the PSR a is a pair of: i) CH1 and CL, ii) Calpha and Cbeta, iii) Cgamma and Cdelta, iv) ligand-binding domain of a receptor and the ligand, or v) PRD (proline rich domain) and SH3 domain.
• the cognate pairing of the scaffold regions are facilitated by introduction of non-native disulfide bonds.
• the scaffold regions are CH1 and CL, Calpha and Cbeta or Cgamma and Cdelta.
• first pair of scaffold regions and the second pair of the scaffold regions are of the same type of scaffold domain, but are configured such that mispairings between the first pair of scaffold regions and the second pair of the scaffold regions are discouraged.
• both the pair of SR a /PSR a and the pair of SR b /PSR b are of the same type, for example, both are CH1/CL domain, or Calpha/Cbeta domain, or Cgamma and Cdelta domain, and the like, but the pair of SR a /PSR a and/or the pair of SR b /PSR b are configured such that mispairing between SR a /PSR b or between SR b /PSR a is discouraged.
• Any suitable means can be used to discourage mispairing between two pairs of scaffold regions, for example, the pair of SR a /PSR a and the pair of SR b /PSR b can be configured to be crossed.
• both the pair of SR a /PSR a and the pair of SR b /PSR b are antibody constant domain formed by CH1/CL pairing.
• the A1/B1 pair and the A2/B2 pair comprise antibody Fab domains, and one of the VH/VL pairs in the Fab domains is crossed to discourage or reduce mispairing between B2 and A1, or between B1 and A2.
• the pair of SR a /PSR a comprises a CH1 domain CH1a and a CL domain CLa
• the pair of SR b /PSR b comprises a CH1 domain CH1b and a CL domain CLb.
• the pair of CH1a/CLa and the pair of CH1b/CLb are configured to be crossed, such that when mispairing between B2 and A1 occurs and/or mispairing between B1 and A2 occurs, it would result in pairing of two non-pairing scaffold regions, such as CH1a and CH1b, or CLa and CLb, which are less stable than mispairing between CH1 and CL, and hence less likely to form.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VH2, and the VA2 comprises VL2, and wherein: a) the PSR a is CL domain CLa, the SR a is CH1 domain CH1a, the SR b is CH1 domain CH1b, the PSR b is CL domain CLb; or b) the PSR a is CH1 domain CH1a, the SR a is CL domain CLa, the SR b is CL domain CLb, the PSR b is CH1 domain CH1b.
• both the first pair of scaffold region and the second pair of the scaffold regions are the same type of scaffold domain, with one or more introduced mutations to reduce or discourage mispairing.
• the VB1 comprises VH1, the VA1 comprises VL1, the VB2 comprises VH2, and the VA2 comprises VL2, the PSR a is CH1 domain CH1a, the SR a is CL domain CLa, the SR b is CH1 domain CH1b, the PSR b is CL domain CLb, and wherein: a) the fusion polypeptide comprises an amino acid sequence of Formula (I) : VH2-CH1b-Linker-VL1-CLa, b) the second polypeptide comprises an amino acid sequence of Formula (II) : VH1-CH1a, c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1b, d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CLb; wherein the pair of CH1b/CLb and/or the pair of CH1a/CLa are configured such that mispairing between CH1b and CLa and/or between CH
• one or more mutations are introduced to the pair of CH1b/CLb and/or the pair of CH1a/CLa, discouraging the mispairing between CH1b and CLa and/or between CH1a and CLb.
• the one or more mutations introduces a non-native binding interaction, such as for example, a non-native covalent bond, a non-native electrostatic interaction, a non-native salt bridge, a non-native hydrophobic-hydrophilic interaction, a non-native connecter, or any combination thereof.
• the non-native binding interaction is introduced to either the pair of CH1b/CLb or the pair of CH1a/CLa, such that mispairing between the pair of CH1b/CLb and the pair of CH1a/CLa is discouraged.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa have one or more characteristics shown below: 1) having at least one non- natural disulfide bond that discourages mispairing between CH1b and CLa and/or between CH1a and CLb; 2) having one or more introduced amino acid mutations that form at least one or more introduced charged amino acid residues that discourages mispairing between CH1b and CLa and/or between CH1a and CLb; or 3) having one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that discourages mispairing between CH1b and CLa or between CH1a and CLb.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa have at least one non-natural disulfide bond that discourages mispairing between CH1b and CLa and/or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected from CH1b/CLb and CH1a/CLa.
• the first CH1/CL pair can be CH1 b /CL b and the second CH1/CL pair can be CH1 a /CL a ; or alternatively, the first CH1/CL pair can be CH1 a /CL a and the second CH1/CL pair can be CH1 b /CL b .
• the first CH1/CL pair is associated by a first disulfide bond that is non-natural.
• the first CH1/CL pair lacks a natural disulfide bond or has a disrupted natural disulfide bond, for example, by mutation of the cysteine residues that form the natural disulfide bond.
• the second CH1/CL pair is associated by a second disulfide bond which is formed at a position different from the first disulfide bond.
• the second disulfide bond formed in the second CH1/CL pair is a natural disulfide bond, or a non-natural disulfide bond.
• the first disulfide bond is formed by two cysteine residues introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain position 134 and light chain position 116, b) heavy chain position 141 and light chain position 116, c) heavy chain position 128 and light chain position 118, d) heavy chain position 126 and light chain position 121, e) heavy chain position 127 and light chain position 121, f) heavy chain position 126 and light chain position 124, g) heavy chain position 170 and light chain position 162, h) heavy chain position 171 and light chain position 162, and i) heavy chain position 173 and light chain position 162.
• the first disulfide bond is formed by two cysteine residues introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: j) heavy chain position 133 and light chain position 209, k) heavy chain position 131 and light chain position 119, l) heavy chain position 133 and light chain position 207, m) heavy chain position 170 and light chain position 176, n) heavy chain position 173 and light chain position 160, o) heavy chain position 133 and light chain position 117, and p) heavy chain position 129 and light chain position 121.
• the first disulfide bond is formed by two cysteine residues introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 126 and light chain EU position 121, b) heavy chain EU position 173 and light chain EU position 160, and c) heavy chain EU position 128 –light chain EU position 118.
• the natural disulfide bond is between heavy chain EU position selected from 131, 219 and 220, and light chain EU position 214. In some embodiments, the natural disulfide bond is between heavy chain EU position 220 and light chain EU position 214.
• the first CH1/CL pair comprises a CH1 comprising substitution at EU position 126 for a cysteine residue, and substitution at EU position 220 for a non-cysteine residue; and a CL comprising substitution at EU position 121 for a cysteine residue, and substitution at EU position 214 for a non-cysteine residue.
• the CH1b comprises substitution at EU position 126 for a cysteine residue, and substitution at EU position 220 for a non-cysteine residue; and the CLb comprises substitution at EU position 121 for a cysteine residue, and substitution at EU position 214 for a non-cysteine residue.
• the CH1b comprises the amino acid sequence of SEQ ID NO: 77, and/or the CLb comprises the amino acid sequence of SEQ ID NO: 79.
• the CH1a comprises substitution at EU position 126 for a cysteine residue, and substitution at EU position 220 for a non-cysteine residue; and the CLa comprises substitution at EU position 121 for a cysteine residue, and substitution at EU position 214 for a non-cysteine residue.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 77, and/or the CLa comprises the amino acid sequence of SEQ ID NO: 79.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa have at least one or more introduced charged amino acid residues that discourages mispairing between CH1b and CLa and/or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected from CH1b/CLb and CH1a/CLa.
• the first CH1/CL pair can be CH1 b /CL b and the second CH1/CL pair can be CH1 a /CL a ; or alternatively, the first CH1/CL pair can be CH1 a /CL a and the second CH1/CL pair can be CH1 b /CL b .
• the first CH1/CL pair contains at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the first CH1/CL pair contains a first pair of oppositely charged residues that favors pairing of first CH1/CL pair.
• the first CH1/CL pair can contain a combination of substitutions, which together provide a first a first pair of oppositely charged residues that favors pairing of first CH1/CL pair.
• a pair of oppositely charged residues can be introduced to the first CH1/CL pair, to facilitate cognate pairing between the CH1 domain and the CL domain in the first CH1/CL pair.
• a charged residue can be introduced to replace a non-charged residue at a position in the CH1 (or CL) , such that the introduced charged residue would form electrostatic interaction with another oppositely charged residue, which is already present or is to be introduced, in the CL (or CH1) , so as to favor pairing of the first CH1/CL pair.
• an existing charged residue at a position in the CH1 (or CL) can be replaced with an oppositely charged residue, such that the charged residue after substitution would form electrostatic interaction with another oppositely charged residue, which is already present or is to be introduced, in the CL (or CH1) , so as to favor pairing of the first CH1/CL pair.
• an existing charged residue in the CH1 or the CL may be replaced with a non-charged residue, to reduce potential interference with the electrostatic interaction between the first CH1/CL pair.
• the second CH1/CL pair contains at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the second CH1/CL pair contains a second pair of oppositely charged residues that favors pairing of second CH1/CL pair, and optionally the first pair of oppositely charged residues and the second pair of oppositely charged residues discourages pairing of CH1a with CLb or CH1b and CLa.
• the first pair of oppositely charged residues and the second pair of oppositely charged residues are configured such that CH1a and CLb have both positive or both negative charged residues at the corresponding positions of the first and the second pair of oppositely charged residues, and/or CH1b and CLa have both positive or both negative charged residues at the corresponding positions of the first and the second pair of oppositely charged residues.
• the first pair of oppositely charged residues and/or the second pair of oppositely charged residues is introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 183 and light chain EU position 176, b) heavy chain EU position 183 and light chain EU position 133, c) heavy chain EU position 147 and light chain EU position 176, d) heavy chain EU position 141 and light chain EU position 116, e) heavy chain EU position 126 and light chain EU position 121, and f) heavy chain EU position 218 and light chain EU position 122.
• the first pair of oppositely charged residues and/or the second pair of oppositely charged residues is introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: g) heavy chain EU position 147 and light chain EU position 131, h) heavy chain EU position 168 and light chain EU position 174, i) heavy chain EU positions 147 and 168, and light chain EU positions 131 and 174.
• the pair of oppositely charged residues comprises a positive-charged amino acid residue and a negative-charged amino acid residue
• the positive-charged amino acid residue is selected from the group consisting of lysine (K) , histidine (H) and arginine (R)
• the negative-charged amino acid residue is selected from the group consisting of aspartic acid (D) and glutamic acid (E) .
• the CH1b comprises substitution of lysine at EU position 147 for a negatively charged residue; the CLb comprises substitution of serine at EU position 176 for a positively charged residue; the CH1a comprises substitution of serine at EU position 183 for a positively charged residue; and the CLa comprises substitution of serine at EU position 176 for a negatively charged residue.
• the CH1b comprises substitution K147D; the CLb comprises substitution S176K; the CH1a comprises substitution S183K; and the CLa comprises substitution S176D.
• the CH1b comprises the amino acid sequence of SEQ ID NO: 88; the CLb comprises the amino acid sequence of SEQ ID NO:90; the CH1a comprises the amino acid sequence of SEQ ID NO: 87; and the CLa comprises the amino acid sequence of SEQ ID NO: 92.
• the CH1a comprises substitution of lysine at EU position 147 for a negatively charged residue; the CLa comprises substitution of serine at EU position 176 for a positively charged residue; the CH1b comprises substitution of serine at EU position 183 for a positively charged residue; and the CLb comprises substitution of serine at EU position 176 for a negatively charged residue.
• the CH1a comprises substitution K147D; the CLa comprises substitution S176K; the CH1b comprises substitution S183K; and the CLb comprises substitution S176D.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 88; the CLa comprises the amino acid sequence of SEQ ID NO: 90; the CH1b comprises the amino acid sequence of SEQ ID NO: 87; and the CLb comprises the amino acid sequence of SEQ ID NO: 92.
• the first CH1/CL pair comprises CH1b and CLb.
• the amino acid residue at EU position 173 is replaced with a cysteine residue
• the amino acid residue at EU position 183 is replaced with a positively charged residue
• the amino acid residue at EU position 220 is replaced with a non-cysteine residue
• the amino acid residue at EU position 160 is replaced with a cysteine residue
• the amino acid residue at EU position 176 is replaced with a negatively charged residue
• the amino acid residue at EU position 214 is replaced with a non-cysteine residue.
• the amino acid residue at EU position 173 is replaced with a cysteine residue
• the amino acid residue at EU position 183 is replaced with a negatively charged residue
• the amino acid residue at EU position 220 is replaced with a non-cysteine residue
• the amino acid residue at EU position 160 is replaced with a cysteine residue
• the amino acid residue at EU position 176 is replaced with a positively charged residue
• the amino acid residue at EU position 214 is replaced with a non-cysteine residue.
• the CH1b comprises substitutions of V173C, S183K, and C220S, and the CLb comprises substitutions of Q160C, S176D, and C214S.
• the CH1b comprises the amino acid sequence of SEQ ID NO: 103; and the CLb comprises the amino acid sequence of SEQ ID NO: 104.
• first CH1/CL pair comprises a combination of at least one non-natural disulfide bond and non-natural electrostatic interaction.
• the first CH1/CL pair comprises CH1a and CLa.
• the amino acid residue at EU position 173 is replaced with a cysteine residue
• the amino acid residue at EU position 183 is replaced with a positively charged residue
• the amino acid residue at EU position 220 is replaced with a non-cysteine residue
• the amino acid residue at EU position 160 is replaced with a cysteine residue
• the amino acid residue at EU position 176 is replaced with a negatively charged residue
• the amino acid residue at EU position 214 is replaced with a non-cysteine residue.
• the CH1a comprises substitutions of V173C, S183K, and C220S
• the CLa comprises substitutions of Q160C, S176D, and C214S.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 103
• the CLa comprises the amino acid sequence of SEQ ID NO: 104.
• the amino acid residue at EU position 173 is replaced with a cysteine residue
• the amino acid residue at EU position 183 is replaced with a negatively charged residue
• the amino acid residue at EU position 220 is replaced with a non-cysteine residue
• the amino acid residue at EU position 160 is replaced with a cysteine residue
• the amino acid residue at EU position 176 is replaced with a positively charged residue
• the amino acid residue at EU position 214 is replaced with a non-cysteine residue.
• At least one of the pair of CH1b/CLb and the pair of CH1a/CLa have one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that discourages mispairing between CH1b and CLa or between CH1a and CLb.
• a first CH1/CL pair and a second CH1/CL pair are selected from CH1b/CLb and CH1a/CLa, and wherein the first CH1/CL pair comprises one or more introduced amino acid mutations that form an orthogonal CH1-CL interface.
• the at least one modification comprises one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that favors pairing of CH1b and CLb, and optionally discourages pairing of CH1b with CLa or CH1a and CLb. In some embodiments, the at least one modification comprises one or more introduced amino acid mutations that form an orthogonal CH1-CL interface that favors pairing of CH1a and CLa, and optionally discourages pairing of CH1a with CLb or CH1b and CLa.
• the orthogonal CH1-CL interface comprises mutations at heavy chain EU positions H168A, F170G, and light chain EU positions L135Y, S176W. In some embodiments, the orthogonal CH1-CL interface comprises mutations at heavy chain EU positions H168A and F170G and light chain EU positions L135Y and S176W, wherein the heavy chain CH1 domain comprises the amino acid sequence of SEQ ID NO: 83, and the light chain CL domain comprises the amino acid sequence of SEQ ID NO: 95.
• the CH1a comprises substitutions of S183E
• the CLa comprises substitutions of V133K
• the CH1b comprises substitutions of A141I, F170S, S181M, S183A, and V185A
• the CLb comprises substitutions of F116A, L235V, S174A, S176F, and T178V.
• the CH1b comprises the amino acid sequence of SEQ ID NO: 68
• the CLb comprises the amino acid sequence of SEQ ID NO: 71.
• the CH1b comprises substitutions of S183E
• the CLb comprises substitutions of V133K
• the CH1a comprises substitutions of A141I, F170S, S181M, S183A, and V185A
• the CLa comprises substitutions of F116A, L235V, S174A, S176F, and T178V.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 68
• the CLa comprises the amino acid sequence of SEQ ID NO: 71.
• the first CH1/CL pair comprises one or more introduced amino acid mutations that form orthogonal Fab designs at a set of heavy chain-light chain EU positions selected from the group consisting of: substitutions at heavy chain EU positions A141I, F170S, S181M, S183A, and V185A, and substitutions at light chain EU positions F116A, A235V, S174A, S176F, and T178V.
• the first CH1/CL pair comprises introduced amino acid mutations that form orthogonal Fab designs at a set of heavy chain-light chain EU positions selected from the group consisting of: substitutions at heavy chain EU positions A141I, F170S, S181M, S183A, and V185A, and substitutions at light chain EU positions F116A, A235V, S174A, S176F, and T178V; and the second CH1/CL pair comprises introduced amino acid mutations that form a pair of oppositely charged residues including S183E in CH1 and V133K in CL.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 68
• the CLa comprises the amino acid sequence of SEQ ID NO: 71
• the CH1b comprises the amino acid sequence of SEQ ID NO: 66
• the CLb comprises the amino acid sequence of SEQ ID NO: 73.
• mutations can also be introduced in variable regions in any one of the VA1/VB1 and VA2/VB2 to discourage the mispairing of B1/A2 or B2/A1.
• a first VH/VL pair and a second VH/VL pair are selected from VH1/VL1 and VH2/VL2, and wherein the first VH/VL pair has at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the first VH/VL contains a third pair of oppositely charged residues that favors pairing of first VH/VL pair.
• the second VH/VL pair has at least one substitution of a non-charged residue to a charged residue, and/or at least one substitution of a charged residue to an oppositely charged residue, such that the second VH/VL contains a fourth pair of oppositely charged residues that favors pairing of second VH/VL pair, and optionally the third pair of oppositely charged residues and the fourth pair of oppositely charged residues discourages pairing of VH1 with VL2 or VH2 and VL1.
• the third pair of oppositely charged residues and the fourth pair of oppositely charged residues are configured such that VH1 and VL2 have both positive or both negative charged residues, and/or VH2 and VL1 have both positive or both negative charged residues.
• the third pair of oppositely charged residues and/or the fourth pair of oppositely charged residues is introduced at a set of heavy chain-light chain EU positions selected from the group consisting of: a) heavy chain EU position 39: light chain EU position 38; b) heavy chain EU position 105: light chain EU position 43, c) heavy chain EU position 62: light chain EU position 1, or d) any combination thereof.
• the pair of oppositely charged residues comprises a positive-charged amino acid residue and a negative-charged amino acid residue
• the positive-charged amino acid residue is selected from the group consisting of lysine (K) , histidine (H) and arginine (R)
• the negative-charged amino acid residue is selected from the group consisting of aspartic acid (D) and glutamic acid (E) .
• the second polypeptide and the third polypeptide further comprise an operably linked first dimerization domain and an operably linked second dimerization domain, respectively, that are associated to form a dimer.
• first dimerization domain and the second dimerization domain comprise CH3 domain of IgG. In some embodiments, the first dimerization domain and the second dimerization domain further comprise a hinge region.
• the second polypeptide comprises an operably linked first dimerization domain, and/or the third polypeptide further comprises an operably linked second dimerization domain. In some embodiments, in the polypeptide complex disclosed herein, the second polypeptide comprises an operably linked second dimerization domain, and/or the third polypeptide further comprises an operably linked first dimerization domain.
• the first dimerization domain comprises an operably linked first Fc region
• the second dimerization domain comprises an operably linked second Fc region.
• the first Fc region and/or the second Fc region is derived from IgG1, IgG2, IgG3 or IgG4.
• the first Fc region and the second Fc region have different amino acid sequences and have at least one configuration that facilitates heterodimerization of the first Fc region and the second Fc region.
• the polypeptide complex disclosed herein comprise one or more amino acid substitution (s) in the interface of the Fc region to facilitate and/or promote heterodimerization.
• modifications comprise introduction of a protuberance into a first Fc region and a cavity into a second Fc region, wherein the protuberance can be positioned in the cavity so as to promote interaction of the first and second Fc polypeptides to form a heterodimer or a complex (also referred to as knob-into-hole structure) .
• Methods of generating antibodies with these modifications are known in the art, e.g. as described in U.S. Pat. No. 5,731,168.
• a “knob” is generated by replacing one or more small amino acid side chains from the interface of the first antibody molecule with larger side chains (e.g., tyrosine or tryptophan) .
• Compensatory “holes” of identical or similar size to the large side chain (s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones (e.g., alanine or threonine) .
• the first Fc region comprises a first Fc mutation
• the second Fc region comprises a second Fc mutation
• the first Fc mutations comprises T366W or S354C
• the second Fc mutation comprises Y349C, T366S, L368A, or Y407V
• the first Fc mutation comprises D399K or E356K
• the second Fc mutation comprises K392D, or K409D
• the first Fc mutation comprises E356K, E357K, or D399K
• the second Fc mutation comprises K370E, K409D, or K439E
• the first Fc mutation comprises S364H, or F405A
• the second Fc mutation comprises Y349T, or T394F
• the first Fc mutation comprises S364H, or T394F
• the second Fc mutation comprises Y394T, or F405A
• the first Fc mutation comprises S364H, or T394F
• the second Fc mutation comprises
• the first Fc region comprises a first Fc mutation
• the second Fc region comprises a second Fc mutation
• the first Fc mutations comprises T366S/L368A/Y407V
• the second Fc mutation comprises T366W
• the first Fc mutations comprises S354C/T366W
• the second Fc mutation comprises Y349C/T366S/L368A/Y407V
• the first Fc mutations comprises T366Y
• the second Fc mutation comprises Y407T
• d) the first Fc mutations comprises T366W
• the second Fc mutation comprises Y407A
• the first Fc mutations comprises T394W
• the second Fc mutation comprises F405A
• the first Fc mutations comprises T366Y/F405A
• the second Fc mutation comprises T394W/Y407T
• the first Fc mutations comprises T366T
• the polypeptide complex disclosed herein comprises a first CH3 region and a second CH3 region, wherein the first CH3 region or the second CH3 region comprises an amino acid sequence differing from wild-type IgG amino acid sequence such that one or more positive-charged amino acids (e.g., lysine, histidine and arginine) in the wild-type human IgG amino acid sequence are replaced with one or more negative-charged amino acids (e.g., aspartic acid and glutamic acid) at the corresponding position (s) in the CH3 region.
• positive-charged amino acids e.g., lysine, histidine and arginine
• negative-charged amino acids e.g., aspartic acid and glutamic acid
• the first CH3 region or the second CH3 region comprises van amino acid sequence differing from wild-type IgG amino acid sequence such that one or more negative-charged amino acids in the wild-type human IgG amino acid sequence are replaced with one or more positive-charged amino acids at the corresponding position (s) in the CH3 region.
• the first Fc region comprises a first Fc mutation
• the second Fc region comprises a second Fc mutation
• the first Fc mutations comprises K370E/D399K/K439D, and the second Fc mutation comprises D356K/E357K/K409D
• the first Fc mutations comprises K409D, and the second Fc mutation comprises D399K
• the first Fc mutations comprises K409E, and the second Fc mutation comprises D399K
• d) the first Fc mutations comprises K409E, and the second Fc mutation comprises D399R
• the first Fc mutations comprises K409D, and the second Fc mutation comprises D399R
• the first Fc mutations comprises D339K
• the second Fc mutation comprises E356K
• g) the first Fc mutations comprises E356K/D399K, and the second Fc mutation comprises K392D/K409D
• the first Fc mutations comprises E356K/D399K
• the “chimeric target binding domain” herein means a recombinant protein that has the variable domains including the complementarity determining regions (CDRs) of an antibody derived from one species, such as a rodent antibody or a murine antibody, while the constant domains of the antibody molecule are derived from those of an antibody of a different species such as human antibody.
• the constant domains of the chimeric antibody may be derived from that of other species, such as a subhuman primate, cat or dog.
• the “humanized target binding domain” herein means a recombinant protein in which the CDRs from an antibody from one species, e.g., a rodent antibody, are transferred into framework regions of a human heavy and light variable domains.
• the constant domains of the antibody molecule are derived from those of a human antibody.
• specific residues of the framework region of the humanized antibody, particularly those that are touching or close to the CDR sequences, may be modified, for example replaced with the corresponding residues from the original rodent, subhuman primate, or other antibody.
• At least one of the first target binding domain and the second target binding domain is fully human, which can be generated using any suitable methods known in the art, for example, from transgenic mice that have been genetically engineered to produce specific human antibodies in response to antigenic challenge. Methods for obtaining human antibodies from transgenic mice are described by Green et al, Nature Genet. 7: 13 (1994) , Lonberg et al, Nature 368: 856 (1994) , and Taylor et al, Int. Immun. 6: 579 (1994) .
• a fully human target binding domain also can be constructed by genetic or chromosomal transfection methods, as well as phage display technology, all of which are known in the art.
• the polypeptide complexes provided herein are based on novel 2: 1 structure, and are bivalent for the second target and monovalent for the first target.
• an additional Fab region is introduced on the basis of the IgG like bispecific antibody, so that one of the two target binding sites would be bivalent and regain its target binding affinity, which works effectively to adjust the affinity difference between two target binding sites without any adversely impact on the antibody specificity or any safety risks.
• affinity difference can be useful, because bivalent binding to the second target confers avidity and allows enhanced differentiation between cells with high and low expression of the second target.
• the comparatively low affinity binding to the first target can be useful to reduce or avoid undesirable effects of strong binding to the first target (for example, non-specific activation of the first target which could lead to unwanted biological effects) .
• the first target binding domain and the second target binding domain bind to different targets.
• at least one of the first target and the second target is a disease-associated antigen.
• a disease-associated antigen can be a tumor-associated antigen, or an antigen associated with an autoimmune diseases or an inflammatory disease, or an antigen associated with an eye disorder, an antigen associated with a central nervous system disease, an antigen associated with an infectious disease, or an antigen associated with a coagulation disorder.
• one of the first target and the second target is a tumor-associated antigen.
• the second target is tumor-associated antigen.
• a tumor-associated antigen is an antigen that is present in a tumor that is not present in normal organs, tissues, and/or cells.
• a tumor-associated antigen is an antigen that is more prevalent in a tumor than in normal organs, tissues, and /or cells.
• a tumor--associated antigen is an antigen that is more prevalent in malignant cancer cells than in normal cells.
• the second target binding domain binds to a tumor-associated antigen.
• a tumor-associated antigen include antigens presented on a tumor cell surface, located on or within tumor cells, presented only by tumor cells and not by normal, i.e. non-tumor cells, representing a protein harboring one or more tumor- specific mutations compared to non-tumor cells, overexpressed in tumor cells when compared to non-tumor cells; accessible for antibody binding in tumor cells due to the less compact structure of the tumor tissue compared to non-tumor tissue, and presented on the vasculature of a tumor, etc. is meant an antigenic substance produced in tumor cells, i.e., it triggers an immune response in the host.
• tumor-associated antigen examples include, but are not limited to: CD19, CD20, CD38, CD30, Her2/neu/ERBB2, CA125, MUC-1, prostate-specific membrane antigen (PSMA) , CD44 surface adhesion molecule, mesothelin, carcinoembryonic antigen (CEA) , epidermal growth factor receptor (EGFR) , EGFRvIII, vascular endothelial growth factor receptor-2 (VEGFR2) , high molecular weight-melanoma associated antigen (HMW-MAA) , MAGE-A1, IL-13R-a2, GD2, and the like.
• PSMA prostate-specific membrane antigen
• CEA carcinoembryonic antigen
• EGFR epidermal growth factor receptor
• EGFRvIII EGFRvIII
• VEGFR2 vascular endothelial growth factor receptor-2
• HMW-MAA high molecular weight-melanoma associated antigen
• MAGE-A1 IL-13R-a
• Cancer-associated antigens also include, e.g., 4-1BB, 5T4, adenocarcinoma antigen, alpha-fetoprotein, BAFF, B-lymphoma cell, C242 antigen, CA-125, carbonic anhydrase 9 (CA-IX) , C-MET, CCR4, CD152, CD19, CD20, CD200, CD22, CD221, CD23 (IgE receptor) , CD28, CD30 (TNFRSF8) , CD33, CD4, CD40, CD44 v6, CD51, CD52, CD56, CD74, CD80, CEA, CNTO888, CTLA-4, DRS, EGFR, EpCAM, CD3, FAP, fibronectin extra domain-B, folate receptor 1, GD2, GD3 ganglioside, glycoprotein 75, GPNMB, HER2/neu, HGF, human scatter factor receptor kinase, IGF-1 receptor, IGF-I, IgG1, L1-CAM, IL-13,
• one of the first target and the second target is a tumor-associated antigen, and the other is an immune related target.
• the first target binding domain binds to an immune-related target.
• the second target is tumor-associated antigen, and the first target binding domain binds to an immune-related target.
• the immune related target provided herein may be selected from the group consisting of CD2, CD3, CD7, CD16, CD27, CD30, CD70, CD83, CD28, CD80 (B7-1) , CD86 (B7-2) , CD40, CD40L (CD154) , CD47, CD122, CD137, CD137L, OX40 (CD134) , OX40L (CD252) , NKG2C, 4-1BB, LIGHT, PVRIG, SLAMF7, HVEM, BAFFR, ICAM-1, 2B4, LFA-1, GITR, ICOS (CD278) , ICOSLG (CD275) , LAG3 (CD223) , A2AR, B7-H3 (CD276) , B7-H4 (VTCN1) , BTLA (CD272) , BTLA, CD160, CTLA-4 (CD152) , IDO1, IDO2, TDO, KIR, LAIR-1, NOX2, PD-1, PD-L1,
• the second target relates to a receptor on the cytotoxic T lymphocytes (e.g. CD3)
• the first target relates to a cell surface tumor antigen such as CD19, CD20, CD33, CD123, HER1, HER2, CEA, disialoganglioside GD2, PSMA, gpA33, EpCAM, P-cadherin, or B7H3 (Sedykh SE, et al., Drug Des Devel Ther. 2018; 12: 195–208. )
• the bispecific polypeptide complexes provided herein can be used to form a link between T cells and tumor cells, which can cause the T cells to exert cytotoxic activity on tumor cells.
• At least one of the first target and the second target is an antigen associated with an autoimmune disease or an inflammatory disease, such as, for example, rheumatoid arthritis (RA) , psoriasis, osteoporosis, idiopathic pulmonary fibrosis, asthma, Sjogren syndrome, Type II diabetes.
• RA rheumatoid arthritis
• psoriasis psoriasis
• osteoporosis idiopathic pulmonary fibrosis
• asthma for example, Sjogren syndrome, Type II diabetes.
• the antigen associated with an autoimmune disease or an inflammatory disease is, for example but not limited to, HSA, TNF, IL6R, IL17A/F, RANKL, IL-13, IL4-IL17, BAFF, ICOSL, IL-17A, NGF, CD32b, CD79b, FGFR1, KLB, AOC3 (VAP-1) , CAM-3001, CCL11 (eotaxin-1) , CD125, CD147 (basigin) , CD154 (CD40L) , CD2, CD20, CD23 (IgE receptor) , CD25 (achain of IL-2 receptor) , CD3, CD4, CD5, IFN- ⁇ , IFN- ⁇ , IgE, IgE Fc region, IL-1, IL-12, IL-23, IL-13, IL-17, IL-17A, IL-22, IL-4, IL-5, IL-5, IL-6, IL-6 receptor, integrin ⁇ 4, integrin
• At least one of the first target and the second target is an antigen associated with an eye disease, such as, for example, age-related macular degeneration (AMD) and diabetic macular edema.
• AMD age-related macular degeneration
• the antigen associated with an eye disease is, for example but not limited to, VEGF or ANG-2.
• At least one of the first target and the second target is an antigen associated with a central nerves system disease, such as, for example, neuroblastoma, glioblastoma, Alzheimer Disease.
• a central nerves system disease such as, for example, neuroblastoma, glioblastoma, Alzheimer Disease.
• the antigen associated with an eye disease is, for example but not limited to, GD2, EGFRvIII, A ⁇ 40, or A ⁇ 42.
• At least one of the first target and the second target is an antigen associated with an infectious disease, such as, for example, pneumonia, virus infection (such as COVID-19 infection) .
• an infectious disease such as, for example, pneumonia, virus infection (such as COVID-19 infection)
• the antigen associated with an autoimmune disease or an inflammatory disease is, for example but not limited to, Psl, Pcrv, or Spike protein of COVID-19 virus.
• At least one of the first target and the second target is an antigen associated with a coagulation disorder, such as, for example, hemophilia A.
• a coagulation disorder such as, for example, hemophilia A.
• the antigen associated with hemophilia A is, for example but not limited to, FIXa, or FX.
• antigen pairs that can be targeted by the bispecific polypeptide complexes provided herein to thereby have potential therapeutic effects can include, but are not limited to, PD-L1: TGF ⁇ , CD38: EGFR, HER2: VEGF, HER2: EGFR, PD-1: CTLA-4, PD-1: TIM3, OX40: PD-L1, FIXa: FX, CD32B: CD79B, Angiopoietin 2: VEGF, IL13: IL4, TNF: IL17A, DLL4: VEGF, IL1 ⁇ : IL1 ⁇ , FAP: DR5, CD30: gpA33, TNF: HSA, IL6R: HSA, IL17A/F: HSA, RANKL: HSA, A ⁇ 40: A ⁇ 42, IL13: IL17, FGFR1: KLB, PsI: PcrV, BAFF: B7RP1, NGF: TNF, and TNF
• the first target is CD3 and the second target is CD20.
• the first target binding domain is capable of binding to CD3, and comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 113, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 114, a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 115, a light chain CDR1 comprising the amino acid sequence of SEQ ID NO: 116, a light chain CDR2 comprising the amino acid sequence of SEQ ID NO: 117, and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO: 118.
• the second target binding domain is capable of binding to CD20, and comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO: 107, a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO: 108, a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO: 109, a light chain CDR1 comprising the amino acid sequence of SEQ ID NO: 110, a light chain CDR2 comprising the amino acid sequence of SEQ ID NO: 111, and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO: 112.
• polypeptide complex comprises: a) the fusion polypeptide comprises an amino acid sequence of Formula (I): VH2-CH1b-Linker-VL1-CLa, b) the second polypeptide comprises an amino acid sequence of Formula (II) : VH1-CH1a, c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1b, d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CLb; wherein the pair of CH1b/CLb and/or the pair of CH1a/CLa are configured such that mispairing between CH1b and CLa and/or between CH1a and CLb is discouraged.
• the CH1a has substitutions at heavy chain EU positions A141I, F170S, S181M, S183A, and V185A
• the CLa has substitutions at light chain EU positions F116A, A235V, S174A, S176F, and T178V
• the CH1b has substitution at heavy chain EU position S183E and the CLb has substitution at light chain EU position V133K
• the CH1 a comprises the amino acid sequence of SEQ ID NO: 68
• the CL a comprises the amino acid sequence of SEQ ID NO: 71
• the CH1 b comprises the amino acid sequence of SEQ ID NO: 66
• the CL b comprises the amino acid sequence of SEQ ID NO: 73.
• the VH1 has substitution at heavy chain EU position Q39E, and the VL1 has substitution at light chain EU position Q38K.
• the VH2 has substitution at heavy chain EU position Q39K, and the VL1 has substitution at light chain EU position Q38E.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 67
• the VL1 comprises the amino acid sequence of SEQ ID NO: 70
• the VH2 comprises the amino acid sequence of SEQ ID NO: 65
• the VL2 comprises the amino acid sequence of SEQ ID NO: 72.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NOs: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 6, 5, 7, and 8.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW1.
• the CH1a has substitution at EU position 126 for a cysteine residue, and substitution at EU position 220 for a non-cysteine residue; the CLa has substitution at EU position 121 for a cysteine residue, and substitution at EU position 214 for a non-cysteine residue.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 77
• the CLa comprises the amino acid sequence of SEQ ID NO: 79
• the CH1b comprises the amino acid sequence of SEQ ID NO: 75
• the CLb comprises the amino acid sequence of SEQ ID NO: 81.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 76
• the VL1 comprises the amino acid sequence of SEQ ID NO: 78
• the VH2 comprises the amino acid sequence of SEQ ID NO: 74
• the VL2 comprises the amino acid sequence of SEQ ID NO: 80.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NOs: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 14, 13, 15, and 16. This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW2.
• the CH1a has substitution at heavy chain EU position K147D, and the CLa has substitutions at light chain EU position S176K; and the CH1b has substitution at heavy chain EU position S183K and the CLb has substitution at light chain EU position S176D.
• the CH1 a comprises the amino acid sequence of SEQ ID NO: 88
• the CL a comprises the amino acid sequence of SEQ ID NO: 90
• the CH1 b comprises the amino acid sequence of SEQ ID NO: 87
• the CL b comprises the amino acid sequence of SEQ ID NO: 92.
• the VH1 has substitution at heavy chain EU positions Q39K and Q105K, and the VL1 has substitutions at light chain EU position Q38D and A43D.
• the VH2 has substitution at heavy chain EU positions Q39D and Q105D, and the VL2 has substitutions at light chain EU position Q38K and A43K.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 85
• the VL1 comprises the amino acid sequence of SEQ ID NO: 89
• the VH2 comprises the amino acid sequence of SEQ ID NO: 86
• the VL2 comprises the amino acid sequence of SEQ ID NO: 91.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NOs: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 22, 21, 23, and 24.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW3.
• the CH1a has substitution at heavy chain EU positions H168A and F170G, and the CLa has substitutions at light chain EU positions L135Y and S176W.
• the CH1 a comprises the amino acid sequence of SEQ ID NO: 83
• the CL a comprises the amino acid sequence of SEQ ID NO: 95
• the CH1 b comprises the amino acid sequence of SEQ ID NO: 75
• the CL b comprises the amino acid sequence of SEQ ID NO: 81.
• the VH1 has substitutions at heavy chain EU positions 62E and Q39K, and the VL1 has substitutions at light chain EU position D1R and Q38D. In certain embodiments, the VH2 has substitution at heavy chain EU position Q39Y, and the VL2 has substitutions at light chain EU position Q38R.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 93
• the VL1 comprises the amino acid sequence of SEQ ID NO: 94
• the VH2 comprises the amino acid sequence of SEQ ID NO: 82
• the VL2 comprises the amino acid sequence of SEQ ID NO: 96.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NOs: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 30, 29, 31, and 32.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW4.
• the CH1a has substitution at EU position 173 for a cysteine residue, substitution at EU position 220 for a non-cysteine residue, and substitution at EU position S183K; the CLa has substitution at EU position 160 for a cysteine residue, substitution at EU position 214 for a non-cysteine residue, and substitution at EU position S176D.
• the CH1a comprises the amino acid sequence of SEQ ID NO: 103
• the CLa comprises the amino acid sequence of SEQ ID NO: 104
• the CH1b comprises the amino acid sequence of SEQ ID NO: 75
• the CLb comprises the amino acid sequence of SEQ ID NO: 81.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 76
• the VL1 comprises the amino acid sequence of SEQ ID NO: 78
• the VH2 comprises the amino acid sequence of SEQ ID NO: 74
• the VL2 comprises the amino acid sequence of SEQ ID NO: 80.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NO: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 54, 53, 55, and 56.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW7.
• polypeptide complex comprises: a) the fusion polypeptide comprises an amino acid sequence of Formula (I) : VH2-CH1 b -Linker-VL1-TCR ⁇ , b) the second polypeptide comprises an amino acid sequence of Formula (II) : VH1-TCR ⁇ , c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1 b , d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CL b .
• the TCR ⁇ comprises the amino acid sequence of SEQ ID NO: 97
• the CH1b comprises the amino acid sequence of SEQ ID NO: 75
• the CLb comprises the amino acid sequence of SEQ ID NO: 81.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 76
• the VL1 comprises the amino acid sequence of SEQ ID NO: 78
• the VH2 comprises the amino acid sequence of SEQ ID NO: 74
• the VL2 comprises the amino acid sequence of SEQ ID NO: 80.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NOs: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 38, 37, 39, and 40.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW5.
• polypeptide complex comprises: a) the fusion polypeptide comprises an amino acid sequence of Formula (I) : VH2-CH1 b -Linker-VH1-CL a , b) the second polypeptide comprises an amino acid sequence of Formula (II) : VL1-CH1 a , c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1 b , d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CL b .
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 76
• the VL1 comprises the amino acid sequence of SEQ ID NO: 78
• the VH2 comprises the amino acid sequence of SEQ ID NO: 74
• the VL2 comprises the amino acid sequence of SEQ ID NO: 80.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NO: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 46, 45, 47, and 48.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW6.
• the polypeptide complex provided herein comprises: a) the fusion polypeptide comprises an amino acid sequence of Formula (I) : VH2-CH1b-Linker-VL1-CH1a, b) the second polypeptide comprises an amino acid sequence of Formula (II) : VH1-CLa, c) the third polypeptide comprises an amino acid sequence of Formula (III) : VH2-CH1b, d) the fourth and fifth polypeptides each comprising an amino acid sequence of Formula (IV) : VL2-CLb.
• the CH1 a comprises the amino acid sequence of SEQ ID NO: 105
• the CLa comprises the amino acid sequence of SEQ ID NO: 106
• the CH1 b comprises the amino acid sequence of SEQ ID NO: 75
• the CLb comprises the amino acid sequence of SEQ ID NO: 81.
• the VH1 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 113, SEQ ID NO: 114 and SEQ ID NO: 115, respectively, and/or the VL1 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 116, SEQ ID NO: 117, and SEQ ID NO: 118, respectively.
• the VH2 comprises a heavy chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 107, SEQ ID NO: 108 and SEQ ID NO: 109, respectively, and/or the VL2 comprises a light chain CDR1/CDR2/CDR3 comprising the amino acid sequences of SEQ ID NO: 110, SEQ ID NO: 111, and SEQ ID NO: 112, respectively.
• the VH1 comprises the amino acid sequence of SEQ ID NO: 76
• the VL1 comprises the amino acid sequence of SEQ ID NO: 78
• the VH2 comprises the amino acid sequence of SEQ ID NO: 74
• the VL2 comprises the amino acid sequence of SEQ ID NO: 80.
• the first Fc and the second Fc comprises the amino acid sequences of SEQ ID NO: 69 and 84.
• the fusion polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide comprise respectively the amino acid sequences of SEQ ID NOs: 62, 61, 63, and 64.
• This polypeptide complex is also referred to as Anti CD20 x CD3 FORMAT NEW8.
• nucleic acid comprising a nucleotide sequence that encodes the fusion polypeptide or the polypeptide complex provided herein.
• nucleic acid or “nucleotide sequence” as used herein refers to deoxyribonucleic acids (DNA) or ribonucleic acids (RNA) and polymers thereof in either single-or double-stranded form. Unless otherwise indicated, a particular polynucleotide sequence also implicitly encompasses conservatively modified variants thereof (e.g. degenerate codon substitutions) , alleles, orthologs, SNPs, and complementary sequences as well as the sequence explicitly indicated.
• degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (see Batzer et al., Nucleic Acid Res. 19: 5081 (1991) ; Ohtsuka et al., J. Biol. Chem. 260: 2605-2608 (1985) ; and Rossolini et al., Mol. Cell. Probes 8: 91-98 (1994) ) .
• the polynucleotides encoding the polypeptide complex disclosed herein may be generated using methods known in the art.
• the sequence of the polynucleotides may be obtained based on the amino acid sequences of the polypeptide complex, and nucleic acids can be generated using synthetic methods.
• the polynucleotides provided herein can also be obtained from another available nucleic acid that encodes a polypeptide with a sequence homologous to the polypeptides in the polypeptide complex disclosed herein. Then a DNA manipulation process can be applied to manipulate the sequence of the parent antibody-encoding nucleic acid, such as introducing mutations, insertion, deletion, etc., so as to obtain the nucleic acid encoding the polypeptide complex disclosed herein.
• the nucleotide sequence that encodes the fusion polypeptide or the polypeptide complex can be inserted into one or more vector (s) for further cloning (amplification of the DNA) or for expression, using recombinant techniques known in the art.
• vectors are available.
• the vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter (e.g., SV40, CMV, EF-1 ⁇ ) , a transcription termination sequence, and one or more other regulatory elements.
• the present disclosure provides vectors comprising the nucleic acid provided herein.
• the nucleic acid provided herein encodes the antibodies, with at least one promoter (e.g., SV40, CMV, EF-1 ⁇ ) operably linked to the nucleic acid sequence, and at least one selection marker.
• at least one promoter e.g., SV40, CMV, EF-1 ⁇
• vectors include, but are not limited to, retrovirus (including lentivirus) , adenovirus, adeno-associated virus, herpesvirus (e.g., herpes simplex virus) , poxvirus, baculovirus, papillomavirus, papovavirus (e.g., SV40) , lambda phage, and M13 phage, plasmid pcDNA3.3, pMD18-T, pOptivec, pCMV, pEGFP, pIRES, pQD-Hyg-GSeu, pALTER, pBAD, pcDNA, pCal, pL, pET, pGEMEX, pGEX, pCI, pEGFT, pSV2, pFUSE, pVITRO, pVIVO, pMAL, pMONO, pSELECT, pUNO, pDUO, Psg5L, pBA
• RTM. pCDM8, pCDNA1.1/amp, pcDNA3.1, pRc/RSV, PCR 2.1, pEF-1, pFB, pSG5, pXT1, pCDEF3, pSVSPORT, pEF-Bos etc.
• Vectors comprising the nucleotide sequence encoding the fusion polypeptide or the polypeptide complex can be introduced to a host cell for cloning or gene expression.
• Suitable host cells for cloning or expressing the DNA in the vectors herein are the prokaryote, yeast, or higher eukaryote cells described above.
• Suitable prokaryotes for this purpose include eubacteria, such as Gram-negative or Gram-positive organisms, for example, Enterobacteriaceae such as Escherichia, e.g., E.
• eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for the polypeptide complex-encoding vectors.
• Saccharomyces cerevisiae, or common baker’s yeast is the most commonly used among lower eukaryotic host microorganisms.
• Kluyveromyces hosts such as, e.g. K. lactis, K. fragilis (ATCC 12, 424) , K. bulgaricus (ATCC 16, 045) , K. wickeramii (ATCC 24, 178) , K.
• waltii ATCC 56, 500
• K. drosophilarum ATCC 36, 906
• K. thermotolerans K. marxianus
• yarrowia EP 402, 226)
• Pichia pastoris EP 183, 070
• Candida Trichoderma reesia
• Neurospora crassa Neurospora crassa
• Schwanniomyces such as Schwanniomyces occidentalis
• filamentous fungi such as, e.g. Neurospora, Penicillium, Tolypocladium, and Aspergillus hosts such as A. nidulans and A. niger.
• Suitable host cells for the expression of glycosylated polypeptide complex are derived from multicellular organisms.
• invertebrate cells include plant and insect cells.
• Numerous baculoviral strains and variants and corresponding permissive insect host cells from hosts such as Spodoptera frugiperda (caterpillar) , Aedes aegypti (mosquito) , Aedes albopictus (mosquito) , Drosophila melanogaster (fruiffly) , and Bombyx mori have been identified.
• a variety of viral strains for transfection are publicly available, e.g., the L-1 variant of Autographa californica NPV and the Bm-5 strain of Bombyx mori NPV, and such viruses may be used as the virus herein according to the present invention, particularly for transfection of Spodoptera frugiperda cells.
• Plant cell cultures of cotton, corn, potato, soybean, petunia, tomato, and tobacco can also be utilized as hosts.
• vertebrate cells have been greatest in vertebrate cells, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure.
• useful mammalian host cell lines are monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651) ; human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol. 36: 59 (1977) ) ; baby hamster kidney cells (BHK, ATCC CCL 10) ; Chinese hamster ovary cells/-DHFR (CHO, Urlaub et al., Proc. Natl. Acad. Sci.
• mice sertoli cells TM4, Mather, Biol. Reprod. 23: 243-251 (1980) ) ; monkey kidney cells (CV1 ATCC CCL 70) ; African green monkey kidney cells (VERO-76, ATCC CRL-1587) ; human cervical carcinoma cells (HELA, ATCC CCL 2) ; canine kidney cells (MDCK, ATCC CCL 34) ; buffalo rat liver cells (BRL 3A, ATCC CRL 1442) ; human lung cells (W138, ATCC CCL 75) ; human liver cells (Hep G2, HB 8065) ; mouse mammary tumor (MMT 060562, ATCC CCL51) ; TRI cells (Mather et al., Annals N.
• the host cell is a mammalian cultured cell line, such as CHO, BHK, NS0, 293 and their derivatives.
• Host cells are transformed with the above-described expression or cloning vectors for antibody production and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.
• the antibodies may be produced by homologous recombination known in the art.
• the host cell is capable of producing the fusion polypeptide or the polypeptide complex provided herein.
• the present disclosure also provides a method of expressing the fusion polypeptide or the polypeptide complex provided herein, comprising culturing the host cell provided herein under the condition at which the vector of the present disclosure is expressed.
• the host cells used to produce the antibodies provided herein may be cultured in a variety of media.
• Commercially available media such as Ham's F10 (Sigma) , Minimal Essential Medium (MEM) , (Sigma) , RPMI-1640 (Sigma) , and Dulbecco's Modified Eagle's Medium (DMEM) , Sigma) are suitable for culturing the host cells.
• any of these media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor) , salts (such as sodium chloride, calcium, magnesium, and phosphate) , buffers (such as HEPES) , nucleotides (such as adenosine and thymidine) , antibiotics (such as GENTAMYCINTM drug) , trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range) , and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to a person skilled in the art.
• the culture conditions such as temperature, pH, and the like, are those previously used with the host cell selected for expression, and will be apparent to a person skilled in the art.
• the fusion polypeptide or the polypeptide complex can be produced intracellularly, in the periplasmic space, or directly secreted into the medium. If the fusion polypeptide or the polypeptide complex is produced intracellularly, as a first step, the particulate debris, either host cells or lysed fragments, is removed, for example, by centrifugation or ultrafiltration. Carter et al., Bio/Technology 10: 163-167 (1992) describe a procedure for isolating antibodies which are secreted to the periplasmic space of E. coli.
• cell paste is thawed in the presence of sodium acetate (pH 3.5) , EDTA, and phenylmethylsulfonylfluoride (PMSF) over about 30 min.
• PMSF phenylmethylsulfonylfluoride
• Cell debris can be removed by centrifugation.
• supernatants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit.
• a protease inhibitor such as PMSF may be included in any of the foregoing steps to inhibit proteolysis and antibiotics may be included to prevent the growth of adventitious contaminants.
• the fusion polypeptide or the polypeptide complex prepared from the cells can be purified using, for example, hydroxylapatite chromatography, gel electrophoresis, dialysis, DEAE-cellulose ion exchange chromatography, ammonium sulfate precipitation, salting out, and affinity chromatography, with affinity chromatography being the preferred purification technique.
• Protein A immobilized on a solid phase is used for immunoaffinity purification of the fusion polypeptide or the polypeptide complex.
• the suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain that is present in the polypeptide complex.
• Protein A can be used to purify antibodies that are based on human gamma1, gamma2, or gamma4 heavy chains (Lindmark et al., J. Immunol. Meth. 62: 1-13 (1983) ) .
• Protein G is recommended for all mouse isotypes and for human gamma3 (Guss et al., EMBO J. 5: 1567 1575 (1986) ) .
• the matrix to which the affinity ligand is attached is most often agarose, but other matrices are available.
• Mechanically stable matrices such as controlled pore glass or poly (styrenedivinyl) benzene allow for faster flow rates and shorter processing times than can be achieved with agarose.
• the polypeptide complex comprises a CH3 domain
• the Bakerbond ABXTM resin J. T. Baker, Phillipsburg, N. J. ) is useful for purification.
• the mixture comprising the fusion polypeptide or the polypeptide complex of interest and contaminants may be subjected to low pH hydrophobic interaction chromatography using an elution buffer at a pH between about 2.5-4.5, preferably performed at low salt concentrations (e.g., from about 0-0.25M salt) .
• the present invention further provides a pharmaceutical composition comprising the polypeptide complex described herein and a pharmaceutically acceptable carrier.
• the term “pharmaceutically acceptable” indicates that the designated carrier, vehicle, diluent, excipient (s) , salt and/or medium is generally chemically and/or physiologically compatible with other ingredients, such as the active ingredient (i.e. the polypeptide complex or the heterodimeric antibody or antigen-binding fragment thereof) comprising the formulation, and is physiologically compatible with a subject receiving the pharmaceutical composition.
• the active ingredient i.e. the polypeptide complex or the heterodimeric antibody or antigen-binding fragment thereof
• a “pharmaceutically acceptable carrier” refers to an ingredient in a pharmaceutical formulation, other than an active ingredient, which is bioactivity acceptable and nontoxic to a subject.
• a pharmaceutical acceptable carrier for use in the pharmaceutical composition disclosed herein may include, for example, pharmaceutically acceptable liquid, gel or solid carriers, aqueous vehicles, nonaqueous vehicles, antimicrobial agents, isotonic agents, buffers, antioxidants, anesthetics, suspending/dispending agents, sequestering or chelating agents, diluents, adjuvants, excipients, or non-toxic auxiliary substances, other components known in the art, or various combinations thereof.
• suitable “components” may include, for example, antioxidants, fillers, binders, disintegrants, buffers, preservatives, lubricants, flavorings, thickeners, coloring agents, emulsifiers or stabilizers such as sugars and cyclodextrins.
• Suitable “antioxidants” may include, for example, methionine, ascorbic acid, EDTA, sodium thiosulfate, platinum, catalase, citric acid, cysteine, thioglycerol, thioglycolic acid, thiosorbitol, butylated hydroxanisol, butylated hydroxytoluene, and/or propyl gallate.
• a pharmaceutical composition that decreases oxidation of the polypeptide complex or heterodimeric antibody or antigen-binding fragment thereof. This reduction in oxidation prevents or reduces loss of binding affinity, thereby improving protein stability and maximizing shelf-life. Therefore, in certain embodiments, a pharmaceutical composition is provided that comprise, in addition to the active ingredient (i.e. the polypeptide complex or the heterodimeric antibody or antigen-binding fragment thereof disclosed herein) , one or more antioxidants such as methionine.
• the pharmaceutical acceptable carriers may include, for example, aqueous vehicles such as sodium chloride injection, Ringer’s injection, isotonic dextrose injection, sterile water injection, or dextrose and lactated Ringer’s injection, nonaqueous vehicles such as fixed oils of vegetable origin, cottonseed oil, corn oil, sesame oil, or peanut oil, antimicrobial agents at bacteriostatic or fungistatic concentrations, isotonic agents such as sodium chloride or dextrose, buffers such as phosphate or citrate buffers, antioxidants such as sodium bisulfate, local anesthetics such as procaine hydrochloride, suspending and dispersing agents such as sodium carboxymethylcelluose, hydroxypropyl methylcellulose, or polyvinylpyrrolidone, emulsifying agents such as Polysorbate 80 (TWEEN-80) , sequestering or chelating agents such as EDTA (ethylenediaminetetraacetic acid) or EGTA (ethylene glyco
• Antimicrobial agents utilized as carriers may be added to pharmaceutical compositions in multiple-dose containers that include phenols or cresols, mercurials, benzyl alcohol, chlorobutanol, methyl and propyl p-hydroxybenzoic acid esters, thimerosal, benzalkonium chloride and benzethonium chloride.
• Suitable excipients may include, for example, water, saline, dextrose, glycerol, or ethanol.
• Suitable non-toxic auxiliary substances may include, for example, wetting or emulsifying agents, pH buffering agents, stabilizers, solubility enhancers, or agents such as sodium acetate, sorbitan monolaurate, triethanolamine oleate, or cyclodextrin.
• Pharmaceutically acceptable “diluents” may include saline and aqueous buffer solutions.
• compositions may include preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of presence of microorganisms may be ensured both by sterilization procedures, supra, and by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol, sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents which delay absorption such as aluminum monostearate and gelatin.
• compositions can be a liquid solution, suspension, emulsion, pill, capsule, tablet, sustained release formulation, or powder.
• Oral formulations can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, polyvinyl pyrollidone, sodium saccharine, cellulose, magnesium carbonate, etc.
• the pharmaceutical compositions are formulated into an injectable composition.
• the injectable pharmaceutical compositions may be prepared in any conventional form, such as for example liquid solution, suspension, emulsion, or solid forms suitable for generating liquid solution, suspension, or emulsion.
• Preparations for injection may include sterile and/or non-pyretic solutions ready for injection, sterile dry soluble products, such as lyophilized powders, ready to be combined with a solvent just prior to use, including hypodermic tablets, sterile suspensions ready for injection, sterile dry insoluble products ready to be combined with a vehicle just prior to use, and sterile and/or non-pyretic emulsions.
• the solutions may be either aqueous or nonaqueous.
• unit-dose parenteral preparations are packaged in an ampoule, a vial or a syringe with a needle. All preparations for parenteral administration should be sterile and not pyretic, as is known and practiced in the art.
• a sterile, lyophilized powder is prepared by dissolving the polypeptide complex as disclosed herein in a suitable solvent.
• the solvent may contain an excipient which improves the stability or other pharmacological components of the powder or reconstituted solution, prepared from the powder. Excipients that may be used include, but are not limited to, water, dextrose, sorbital, fructose, corn syrup, xylitol, glycerin, glucose, sucrose or other suitable agents.
• the solvent may contain a buffer, such as citrate, sodium or potassium phosphate or other such buffer known to those of skill in the art at, in one embodiment, about neutral pH.
• the resulting solution will be apportioned into vials for lyophilization.
• Each vial can contain a single dosage or multiple dosages of the polypeptide complex, the polypeptide complex. Overfilling vials with a small amount above that needed for a dose or set of doses (e.g., about 10%) is acceptable so as to facilitate accurate sample withdrawal and accurate dosing.
• the lyophilized powder can be stored under appropriate conditions, such as at about 4 °C to room temperature.
• Reconstitution of a lyophilized powder with water for injection provides a formulation for use in parenteral administration.
• the sterile and/or non-pyretic water or other liquid suitable carrier is added to lyophilized powder. The precise amount depends upon the selected therapy being given, and can be empirically determined.
• a composition comprising a pharmaceutically acceptable carrier, diluent or adjuvant, and an active ingredient.
• the active ingredient can be the polypeptide complex disclosed herein, or a conjugate of the polypeptide complex disclosed herein.
• polypeptide complex as provided herein can be used in a non-conjugated form or in a conjugated form.
• the polypeptide complexes are conjugated to one or more desired conjugate moieties, i.e. heterologous moieties, to realize certain functionalities, e.g. to facilitate target detection or for imaging or therapy.
• desired conjugate moieties i.e. heterologous moieties
• the present disclosure provides a conjugate, which comprises the polypeptide complex provided herein, and a conjugate moiety (e.g. payload) that is conjugated thereto.
• the payload can be any one of the group consisting of a radioactive label, a fluorescent label, an enzyme-substrate label, an affinity purification tag, a tracer molecule, an anticancer drug, and a cytotoxic molecule.
• conjugates can be linked to the engineered antibody provided herein by covalent binding, affinity binding, intercalation, coordinate binding, complexation, association, blending, or addition, among others.
• Conjugate Vaccines Contributions to Microbiology and Immunology, J. M. Cruse and R. E. Lewis, Jr. (eds. ) , Carger Press, New York, (1989) .
• the polypeptide complex provided herein may be engineered to contain specific sites outside the epitope binding portion that may be specifically utilized for binding to one or more conjugates.
• a site may include one or more reactive amino acid residues, such as for example cysteine or histidine residues, to facilitate covalent linkage to a conjugate.
• the N-terminus and/or C-terminus of the polypeptide complex provided herein can also serve to provide reactive groups for conjugation.
• the N-terminus can be conjugated to one moiety (e.g. polyethylene glycol (PEG) , etc. ) and the C-terminus is conjugated to another moiety (e.g. biotin, etc. ) .
• PEG polyethylene glycol
• polypeptide complex provided herein may be linked to a conjugate directly, or indirectly for example through another conjugate or through a linker.
• the polypeptide complex provided herein having a reactive residue such as cysteine may be linked to a thiol-reactive agent in which the reactive group is, for example, a maleimide, an iodoacetamide, a pyridyl disulfide, or other thiol-reactive conjugation partner (Haugland, 2003, Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Inc.; Brinkley, 1992, Bioconjugate Chem. 3: 2; Garman, 1997, Non-Radioactive Labelling: A Practical Approach, Academic Press, London; Means (1990) Bioconjugate Chem. 1: 2; Hermanson, G. in Bioconjugate Techniques (1996) Academic Press, San Diego, pp. 40-55, 643-671) .
• the reactive group is, for example, a maleimide, an iodoacetamide, a pyridyl disulfide, or other thiol-reactive conjugation partner
• polypeptide complex provided herein may be conjugated to biotin, then indirectly conjugated to a second conjugate that is conjugated to avidin.
• polypeptide complex may be linked to a linker which further links to the conjugate.
• linkers include bifunctional coupling agents such as N-succinimidyl-3- (2-pyridyldithio) propionate (SPDP) , succinimidyl-4- (N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) , iminothiolane (IT) , bifunctional derivatives of imidoesters (such as dimethyl adipimidate HCl) , active esters (such as disuccinimidyl suherate) , aldehydes (such as glutaraldehyde) , bis-azido compounds (such as bis (p-azidobenzoyl) hexanediamine) , bis-diazonium derivatives (such as bis- (p-diazoniumbenzoyl) -ethylenediamine) , diisocyanates (such as toluene 2, 6-diisocyanate) , and his-active fluorine compounds (such as
• Particularly preferred coupling agents include N-succinimidyl-3- (2-pyridyldithio) propionate (SPDP) (Carlsson et al., Biochem. J. 173: 723-737 (1978) ) and N-succinimidyl-4- (2-pyridylthio) pentanoate (SPP) to provide for a disulfide linkage.
• SPDP N-succinimidyl-3- (2-pyridyldithio) propionate
• SPP N-succinimidyl-4- (2-pyridylthio) pentanoate
• the conjugate moiety comprises an agent for detection or isolation, such as a clearance-modifying agent, a chemotherapeutic agent, a toxin, a radioactive isotope, a lanthanide, a luminescent label, a fluorescent label, an enzyme-substrate label, a DNA-alkylator, a topoisomerase inhibitor, a tubulin-binder, or other anticancer drugs.
• an agent for detection or isolation such as a clearance-modifying agent, a chemotherapeutic agent, a toxin, a radioactive isotope, a lanthanide, a luminescent label, a fluorescent label, an enzyme-substrate label, a DNA-alkylator, a topoisomerase inhibitor, a tubulin-binder, or other anticancer drugs.
• the conjugate moiety can be a detectable label, a pharmacokinetic modifying moiety, a purification moiety, a cytotoxic moiety or a therapeutic agent.
• detectable label may include a fluorescent labels (e.g. fluorescein, rhodamine, dansyl, phycoerythrin, or Texas Red) , enzyme-substrate labels (e.g. horseradish peroxidase, alkaline phosphatase, luceriferases, glucoamylase, lysozyme, saccharide oxidases or ⁇ -D-galactosidase) , radioisotopes (e.g.
• the conjugate moiety can be a pharmacokinetic modifying moiety such as PEG which helps increase half-life of the antibody.
• suitable polymers include, such as, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinyl pyrrolidone, copolymers of ethylene glycol/propylene glycol, and the like.
• the polymer may be of any molecular weight and may be branched or unbranched.
• the number of polymers attached to the antibody may vary, and if more than one polymer is attached, they can be the same or different molecules.
• the conjugate can be a purification moiety such as a magnetic bead.
• the conjugate moiety can be a cytotoxic moiety.
• a “cytotoxic moiety” can be any agent that is detrimental to cells or that can damage or kill cells. Examples of cytotoxic moiety include, without limitation, taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, puromycin and analogs thereof, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil decarbazine) , antimetabol
• the conjugate moiety comprises an enzymatically active toxin or a fragment thereof, including but not limited to diphtheria A chain, non-binding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa) , ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins, Momordica charantia inhibitor, curcin, crotin, Sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes.
• an enzymatically active toxin or a fragment thereof including but not limited to diphtheria A chain, non-binding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginos
• polypeptide complex provided herein are used as a base for a conjugate.
• the present invention provides a composition comprising the polypeptide complex or the conjugate described herein and a pharmaceutically acceptable carrier.
• the present invention provides a method for treating a disease condition in a subject that is in need of such treatment, comprising: administering to the subject a therapeutically effective amount of the polypeptide complex of the present invention, the pharmaceutical composition described herein, the conjugate described herein, or the composition described herein.
• the term “subject” or “individual” or “animal” or “patient” refers to human or non-human animal, including a mammal or a primate, in need of diagnosis, prognosis, amelioration, prevention and/or treatment of a disease or disorder.
• Mammalian subjects include humans, domestic animals, farm animals, and zoo, sports, or pet animals such as dogs, cats, guinea pigs, rabbits, rats, mice, horses, swine, cows, bears, and so on.
• the subject is human.
• treatment may include, alleviating a condition, slowing the onset or rate of development of a condition, delaying the development of symptoms associated with a condition, reducing or ending symptoms associated with a condition, generating a complete or partial regression of a condition, or some combinations thereof.
• disorder refers to a condition that affects a subject who would nonetheless benefits from treatment with the polypeptide complex.
• the term “therapeutically effective amount” of a therapeutic agent refers to an amount of the therapeutic agent that, when taken by a subject in an appropriate manner, can generate sufficient therapeutic effects to the subject. It is to be understood that just like other therapeutic drugs, the therapeutically effective amount of the polypeptide complex as provided above will be influenced by various factors known in the art, such as for example body weight, age, past medical history, present medications, state of health of the subject and potential for cross-reaction, allergies, sensitivities and adverse side-effects, as well as the administration route and extent of disease development. Dosages may be proportionally reduced or increased by one of ordinary skill in the art (e.g., physician or veterinarian) as indicated by these and other circumstances or requirements.
• the polypeptide complex as provided herein may be administered at a therapeutically effective amount of about 0.01 mg/kg to about 100 mg/kg. Dosage regimens may be adjusted to provide the optimum desired response (e.g., a therapeutic response) . For example, a single dose may be administered, or several divided doses may be administered over time.
• the polypeptide complex described above and the method disclosed herein can be applied to treat a wide variety of diseases.
• the diseases that are contemplated to be treatable by the polypeptide complex described above and the method disclosed herein can include the following:
• cancers and other hyperproliferative disorders including both benign or malignant tumors, leukemia and lymphoid malignancies.
• examples include neuronal, glial, astrocytal, hypothalamic, glandular, macrophagal, epithelial, endothelial, and stromal malignancies.
• examples include: cancers of the head, neck, eye, mouth, throat, esophagus, chest, skin, bone, lung, colon, rectum, colorectal, stomach, spleen, kidney, skeletal muscle, subcutaneous tissue, metastatic melanoma, endometrial, prostate, breast, ovaries, testicles, thyroid, blood, lymph nodes, kidney, liver, pancreas, brain, or central nervous system;
• autoimmune and/or inflammatory disorders including alopecia areata, ankylosing spondylitis, antiphospholipid syndrome, autoimmune Addison's disease, autoimmune diseases of the adrenal gland, autoimmune hemolytic anemia, autoimmune hepatitis, autoimmune oophoritis and orchitis, Sjogren’s syndrome, psoriasis, atherosclerosis, diabetic and other retinopathies, retrolental fibroplasia, age-related macular degeneration, neovascular glaucoma, hemangiomas, thyroid hyperplasias (including Grave’s disease) , corneal and other tissue transplantation, and chronic inflammation, sepsis, rheumatoid arthritis, peritonitis, Crohn’s disease, reperfusion injury, septicemia, endotoxic shock, cystic fibrosis, endocarditis, psoriasis, arthritis (e.g., psoriatic arthritis) , anaphylactic
• autoimmune thrombocytopenia Behcet’s disease, bullous pemphigoid, cardiomyopathy, celiac sprue-dermatitis, chronic fatigue immune dysfunction syndrome (CFIDS) , chronic inflammatory demyelinating polyneuropathy, Churg-Strauss syndrome, cicatrical pemphigoid, CREST syndrome, cold agglutinin disease, discoid lupus, essential mixed cryoglobulinemia, fibromyalgia-fibromyositis, glomerulonephritis, Guillain-Barre, Hashimoto’s thyroiditis, idiopathic pulmonary fibrosis, idiopathic thrombocytopenia purpura (ITP) , IgA neuropathy, juvenile arthritis, lichen planus, lupus erythematosus, Meniere’s disease, mixed connective tissue disease, multiple sclerosis, type 1 or immune-mediated diabetes mellitus, myasthenia gravis
• Inflammatory disorders can further include, but are not limited to, asthma, encephilitis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD) , allergic disorders, septic shock, pulmonary fibrosis, undifferentitated spondyloarthropathy, undifferentiated arthropathy, arthritis, inflammatory osteolysis, and chronic inflammation resulting from chronic viral or bacterial infections;
• COPD chronic obstructive pulmonary disease
• infectious and parasitic diseases such as those caused by viruses (e.g. HBV, HCV, HIV, RSV, hMPV, PIV, coronaviruses, or influenza viruses, etc. ) , fungi (e.g. Naegleria, Aspergillus, Blastomyces, Histoplasma, Candida or Tinea genera, etc. ) , eukaryotic microbes (e.g. Giardia, Toxoplasma, Plasmodium, Trypanosoma, and Entamoeba genera, etc. ) , and bacteria (Staphylococcus, Streptococcus, Pseudomonas, Clostridium, Borrelia, Vibro and Neiserria genera, etc. ) ;
• viruses e.g. HBV, HCV, HIV, RSV, hMPV, PIV, coronaviruses, or influenza viruses, etc.
• fungi e.g. Naegleria, As
• the disease is selected from the group consisting of a cancer, an inflammatory disease, an infectious or parasitic disease, a cardiovascular disease, eye disease, central nerves system (CNS) disease, an injury, metabolic disease, autoimmune disease, or a coagulation disorder.
• the CNS disease is neuropathy, a neuropsychiatric condition, neuroblastoma, glioblastoma, or Alzheimer Disease.
• polypeptide complex disclosed herein may be administered by any route known in the art, such as for example parenteral (e.g., subcutaneous, intraperitoneal, intravenous, including intravenous infusion, intramuscular, or intradermal injection) or non-parenteral (e.g., oral, intranasal, intraocular, sublingual, rectal, or topical) routes.
• parenteral e.g., subcutaneous, intraperitoneal, intravenous, including intravenous infusion, intramuscular, or intradermal injection
• non-parenteral e.g., oral, intranasal, intraocular, sublingual, rectal, or topical routes.
• the polypeptide complex disclosed herein may be administered alone or in combination with one or more additional therapeutic means or agents.
• the polypeptide complex disclosed herein may be administered in combination with another therapeutic agent, for example, a chemotherapeutic agent or an anti-cancer drug.
• the polypeptide complex as disclosed herein that is administered in combination with one or more additional therapeutic agents may be administered simultaneously with the one or more additional therapeutic agents, and in certain of these embodiments the polypeptide complex and the additional therapeutic agent (s) may be administered as part of the same pharmaceutical composition.
• the polypeptide complex administered “in combination” with another therapeutic agent does not have to be administered simultaneously with or in the same composition as the agent.
• the polypeptide complex administered prior to or after another agent is considered to be administered “in combination” with that agent as the phrase is used herein, even if the polypeptide complex and second agent are administered via different routes.
• additional therapeutic agents administered in combination with the polypeptide complex disclosed herein are administered according to the schedule listed in the product information sheet of the additional therapeutic agent, or according to the Physicians' Desk Reference 2003 (Physicians' Desk Reference, 57th Ed; Medical Economics Company; ISBN: 1563634457; 57th edition (November 2002) ) or protocols well known in the art.
• the present disclosure provides a method of detecting presence or amount of an antigen in a sample.
• the method comprises contacting a sample suspected of containing the antigen with the polypeptide complex described herein and determining the formation of a complex between the antigen and the polypeptide complex.
• the antigen is a disease-associated antigen, for example, a tumor-associated antigen.
• the polypeptide complex disclosed herein is used in a method of diagnosing a subject suffering from a disease (e.g. cancer) , the method comprising: determining the presence or amount of the disease-related (e.g. tumor-related) antigen in a sample obtained from the subject by contacting the sample with a polypeptide complex of the disclosure and detecting the presence of the antigen bound polypeptide complex.
• Any sample suspected of containing the tumor-associated antigen can be used, such as a biological fluid such as a blood sample, a plasma sample, a urine sample, and the like, as well as biopsy of a disease tissue (e.g. tumor tissue) .
• a biological fluid such as a blood sample, a plasma sample, a urine sample, and the like
• biopsy of a disease tissue e.g. tumor tissue
• the presence or level of the disease-associated (e.g. tumor-associated) antigen in a sample can be determined based on the detection of the presence or level of the complex of the disease-associated (e.g. tumor-associated) antigen bound by the polypeptide complex or the antigen binding fragment thereof disclosed herein. Any suitable methods can be used for such detection, for example, by immunoassays such as immunohistochemistry (IHC) , immunofluorescence (IF) , immunoblotting (e.g., Western blotting) , flow cytometry (e.g., 15 FACS TM ) , Enzyme-linked Immunosorbent Assay (ELISA) , enzyme immunoassay (EIA) , and radioimmunoassay (RIA) .
• immunohistochemistry IHC
• IF immunofluorescence
• IF immunoblotting
• flow cytometry e.g., 15 FACS TM
• ELISA Enzyme-linked Immunosorbent Assay
• EIA enzyme
• the polypeptide complex disclosed herein are detectably labeled by a conjugated payload, or are not labeled but can react with a second molecule which is detectably labeled (e.g. a detectably labeled secondary antibody) .
• the polypeptide complex disclosed herein may be immobilized on a solid substrate.
• the immobilization can be via covalent linking or non-covalent attachment (e.g. coating) .
• solid substrate include porous and non-porous materials, latex particles, magnetic particles, microparticles, strips, beads, membranes, microtiter wells and plastic tubes. The choice of solid phase material and method of detectably labeling can be determined based upon desired assay format performance characteristics.
• the level of an antigen can be determined, for example, by normalizing to a control value or to a standard curve.
• the control value can be predetermined or determined concurrently.
• the assays and methods provided herein for the measurement of the level of an antigen can be adapted or optimized for use in automated and semi-automated systems or point of care assay systems.
• Bispecific antibodies were constructed using both the new 2: 1 format disclosed in the present invention (i.e. designated as FORMAT NEW below, and shown in Figure 5A) , and the existing 2: 1 format known in the art (i.e. designated as FORMAT EX below, and shown in Figure 4A) .
• FORMAT NEW the new 2: 1 format disclosed in the present invention
• FORMAT EX the existing 2: 1 format known in the art
• 8 pairs of bispecific antibodies were constructed and expressed, which are otherwise identical except for using the FORMAT EX or using the FORMAT NEW, so as to allow head-to-head comparison of the two formats.
• Both the FORMAT NEW and FORMAT EX are IgG like bispecific antibodies, which are composed mainly of three Fab domains derived from two monoclonal antibodies, in which one Fab domain is derived from the first antibody, and the other two Fab domains are derived from the second antibody ( Figure 4A and Figure 5A) .
• the first antibody heavy chain was named B1 (including VH1 and CH1a) and the first antibody light chain was named A1 (including VL1 and CLa) , which is monovalent in the bispecific antibodies.
• the second antibody heavy chain was named B2 (including VH2 and CH1b) and the second antibody light chain was named A2 (including VL2+CLb) , which are bivalent in the bispecific antibodies.
• the linker (GGGGS) 2 was used in the bispecific antibodies to link different functional domains.
• the anti-CD20 antibody were made to be bivalent (i.e. corresponding to A2 and B2 in the Figures 4A and Figure 5A)
• the anti-CD3 was monovalent (i.e. corresponding to A1 and B1 in the Figures 4A and Figure 5A)
• the anti-CD20 antibody used in this example is 2F2, and has been previously reported in US20040167319.
• the anti-CD3 antibody used in this example is SP34, and has been previously reported in The EMBO Journal vol. 4 no. 2 pp. 337-344, 1985
• CH3 regions were also introduced in CH3 regions to promote heterodimerization of the first Fc region and the second Fc region. Specifically, one of the CH3 regions introduced T366S, L368A, and Y407V, while the other one was introduced T366W mutation.
• the mutations of CH3 regions in the following examples provided for the knob-into-hole design in the bispecific antibodies.
• bispecific antibody structure indicated above 8 pairs of bispecific antibodies were constructed and expressed by using the novel 2: 1 format of the present invention: FORMAT NEW (as shown in Figure 4A) , and by using the conventional 2: 1 format: FORMAT EX (as shown in Figure 5A) .
• These 8 pairs of bispecific antibodies i.e. a total of 16 bispecific antibodies
• FORMAT NEW1 to FORMAT NEW8 8 pairs of bispecific antibodies
• FORMAT EX1 to FORMAT EX8 8 pairs of bispecific antibodies
• Plasmids were constructed to encode each of the polypeptide chains of each of the above bispecific antibodies.
• the plasmids encoding for each bispecific antibody were transfected into ExpiCHO-Scells to allow expression of the polypeptide chains followed by assembly into the respective bispecific antibody.
• the expressed bispecific antibodies were harvested and purified with Protein-A. The specific experimental procedures are described below.
• the gene sequences encoding the polypeptide chains were codon optimized using OptimumGene before proceeding to synthesis.
• the gene sequences were constructed in pcDNA3.4 vector, and the confirmed recombinant vector DNA was prepared for transfection by PureLink TM Hipure.
• the cells were cultured in a CO 2 shaker using ExpiCHO Expression Medium, and when the ExpiCHO-Scell density reached 7 ⁇ 10 6 -10 ⁇ 10 6 viable cells/ml and the viability rate was >95%, 25 ml of the cell suspension were transfected with ExpiFectamine TM CHO/DNA complexes containing approximately 25ul of DNA heavy chains and light chains.
• the purified antibodies were analyzed and identified by SEC-HPLC (see Figures 7A to 7P) and SDS-PAGE (see Figures 6A and 6B) , respectively.
• SDS-PAGE The sample was mixed with 4X sample buffer (15 ⁇ l + 5 ⁇ l) in an Eppendorf tube and heated at 100°C for 5-10min, centrifuged, with the supernatant collected. Then, 5 ⁇ g of the treated sample was taken with a pipette and carefully added to each well of the gel sequentially, and marker was added to one of the wells.
• the electrode chamber was placed on the electrophoresis tank, with the power on, the positive and negative poles aligned, and the voltage set to 150 V. After running for 5 min, the voltage was adjusted to 200 V. Electrophoresis was run again for about 35 min and then turn off the power to stop.
• a Bio-rad gel imager was used to scan and take pictures of the non-stained SDS-PAGE gels, and the pictures were analyzed using Image Lab5 . 2 . 1 to calculate protein purity.
• CE-SDS Capillary electrophoresis sodium dodecyl sulfate
• Mobile phase B ultrapure water
• the anti-CD20 x CD3 bispecific molecule FORMAT New7 was constructed and purified using conventional methods, demonstrating the convenience of the present invention for purification.
• Chain 1 (AL) : B2-linker-A1 (MW: about 50kDa)
• Chain 4 (BL) : A2 (MW: about 25kDa)
• Anti-CD20 x CD3 FORMAT New7 is a bispecific antibody, consisting of 2 pairs of different light and heavy chains. Two stable transfer vectors were used to co-transfect host cells with CHO-K1 cells. After transfection, cells were plated at a density of 2x 10e4 cells/well, and cultured in the presence of selection pressure in a CO 2 incubator. After about 3 weeks of culture, supernatants were taken from the cell culture to test for binding to CD20 and CD3, respectively. The supernatants showing high binding activity to CD20 and CD3 were identified, and the corresponding cell clones were expanded into 24-well cell culture plates. After 4 to 6 days, supernatants were tested for binding activity to CD20 and CD3.
• Cell clones with higher binding activity to CD20 and CD3 were expanded, and finally the stable-transfected cell lines were selected and expanded.
• Stable cell lines were passaged and cultured in shake flasks for 3 days, tested for binding activity to CD20 and CD3, and Fed-batch evaluation and cryopreservation were performed based on cell growth and expression.
• the expression and quality of the Feb-batch samples were tested (SEC, non-reduced CE-SDS, CEX, SDS-PAGE) to evaluate the growth, metabolism, productivity and product quality of the stable cell pool, and the stable cell pool was finally selected for subsequent monoclonal cell line screening.
• the cell line mentioned above was plated into 96-well plates by single cell sorter with 1 cell per well, cultured in CO 2 incubator and photographed by sweeping the plates with Solentim Cell Metric. Monoclonal cells were selected for Fed-batch evaluation and cell cryopreservation. The expression and quality analysis (SEC, nrCE-SDS, iCIEF, N-Glycan, molecular weight) were performed to evaluate the growth, metabolism, productivity and product quality of the stable cell pool, and 6 monoclonal cell lines were selected for RCB banking.
• the selected monoclonal cell lines were revived, amplified, inoculated and cultured in shake flasks for 14 days. The supernatants were harvested for purification and analysis.
• SDS-PAGE The supernatant sample was mixed with 4X sample buffer (15 ⁇ l + 5 ⁇ l) in an Eppendorf tube and heated at 100°C for 5-10min, centrifuged, with the supernatant collected. Then, 5 ⁇ g of the treated sample was taken with a pipette and carefully added to each well of the gel sequentially, and marker was added to one of the wells.
• the electrode chamber was placed on the electrophoresis tank, with the power on, the positive and negative poles aligned, and the voltage set to 150 V. After running for 5 min, the voltage was adjusted to 200 V. Electrophoresis was run again for about 35 min and then turn off the power to stop.
• a Bio-rad gel imager was used to scan and take pictures of the non-stained SDS-PAGE gels, and the pictures were analyzed using Image Lab5 . 2 . 1 to calculate protein purity.
• CE-SDS The sample was diluted to 2.5 mg/ml with ultrapure water and added to 55 ⁇ L Sample Buffer, followed by 2 ⁇ L 10 kDa IS and 5 ⁇ L 250 mM IAM, and mixed well. The samples were next heated at 70 ⁇ 2 °C for 10 ⁇ 2 min, then cooled to room temperature and transferred to the accompanying sample tube, labeled and ready for sampling.
• Mobile phase B ultrapure water
• the expression product purity of the anti-CD20 x CD3 FORMAT New7 bispecific antibody was about 84.1%using SDS-PAGE analysis ( Figure 8) .
• the expression product purity of the anti-CD20 x CD3 FORMAT New7 bispecific antibody was about 82.91%using CE-SDS analysis (see, peak #10 in Figure 9) .
• the purity of the expressed anti-CD20 x CD3 FORMAT New7 bispecific antibody was about 90.23%using SEC-HPLC analysis (see, peak No 4 in Figure 10) .
• LMW Low Molecular Weight
• HMW High Molecular Weight
• Affinity chromatography The supernatant samples containing the anti-CD20 x CD3 FORMAT New7 bispecific antibody obtained from the cell culture were eluted by affinity chromatography at pH 4.0, and the elution peaks were subsequently analyzed.
• CEX Cation exchange chromatography
• EQ/wash/Elution Buffer A 50mM NaAc-HAc, pH 6.
• Elution Buffer B 50mM NaAc-HAc+1M NaCl, pH6.
• the purification profile is shown in Figure 11. As shown in Figure 11, the target product was successfully eluted from the affinity chromatography column by the eluent buffer, and a single high peak was detected using UV signal (see the arrow in Figure 11) , indicating that the purified product has high purity.
• the elute was analyzed by SEC-HPLC for purity, and the result shows the purity was 92.55% (see, peak #3 of Figure 12) , which indicates that AC reduced the LMW content.
• the elute was also analyzed by SDS-PAGE for purity, and the result shows the purity was 85.7% (see Figure 13) , which indicates that there were still LMW after purification in this step ( Figure 13) .
• the elute from the AC process was collected and further purified using CEX.
• the elution profile after CEX purification is shown in Figure 14. A major single peak was detected by UV signal (see the peak spanning from the 100ml to the 120ml elute) , which indicates that target product was successfully eluted by the eluent buffer by linear gradient elution.
• the novel 2: 1 bispecific antibody structure of this invention (FORMAT NEW) can be readily purified using conventional purification methods such as AC and CEX.
• a purification process as simple as two steps can provide a high purity sample with a purity of over 95%, which indicated that the significant advantage of FORMAT NEW antibodies over the FORMAT EX antibodies in antibody purification, and also suggests the ease of use of the FORMAT NEW antibody structure of the present invention.
• the examples used anti-CD20 x CD3 bispecific antibodies to demonstrate the convenience of purification and high purity
• the inventors have also tested other bispecific antibodies constructed with the new bispecific antibody format provided in the present disclosure, and obtained similar results in convenience of purification and high purity. Therefore, it should be understood that the new format provided in the present disclosure is applicable on various kinds of targets and can provide for convenience of purification and high purity for any bispecific antibodies.

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