EP4208164A1 - Composition comprising cannabinoids, and/or terpens, and methods of using same - Google Patents

Composition comprising cannabinoids, and/or terpens, and methods of using same

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Publication number
EP4208164A1
EP4208164A1 EP21863840.1A EP21863840A EP4208164A1 EP 4208164 A1 EP4208164 A1 EP 4208164A1 EP 21863840 A EP21863840 A EP 21863840A EP 4208164 A1 EP4208164 A1 EP 4208164A1
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EP
European Patent Office
Prior art keywords
administering
cbd
pharmaceutical composition
subject
cannabinoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21863840.1A
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German (de)
English (en)
French (fr)
Other versions
EP4208164A4 (en
Inventor
Sheila LANGIER
Neta RIMMERMAN
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Medicane Research & Development Ltd
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Medicane Research & Development Ltd
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Publication date
Application filed by Medicane Research & Development Ltd filed Critical Medicane Research & Development Ltd
Publication of EP4208164A1 publication Critical patent/EP4208164A1/en
Publication of EP4208164A4 publication Critical patent/EP4208164A4/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/05—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0043—Nose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/01—Hydrocarbons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/01—Hydrocarbons
    • A61K31/015—Hydrocarbons carbocyclic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/57—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/57—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
    • A61K31/573—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/658—Medicinal preparations containing organic active ingredients o-phenolic cannabinoids, e.g. cannabidiol, cannabigerolic acid, cannabichromene or tetrahydrocannabinol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/02—Nasal agents, e.g. decongestants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders

Definitions

  • the present invention relates to cannabinoid compounds, pharmaceutical compositions comprising same, and method of use thereof, such as for treating a chronic inflammatory disease.
  • Chronic rhinosinusitis with nasal polyps is a chronic inflammatory disease of the sinonasal tissues. It is characterized by inflammatory tissue growth in nasal and paranasal cavity, which leads to nasal obstruction, secretion, loss of the sense of smell, anosmia, headaches, facial pain and reduced general well-being.
  • Nasal polyps (NP) are believed to be a multifactorial disease usually associated with asthma and occasionally associated with aspirin sensitivity. It is usually a type 2 inflammatory disease with increased interleukin (IL) -5, -4 and -13, eosinophil infiltrate and local immunoglobulin (Ig) E production.
  • NP treatments usually include corticosteroids and/or surgery, in which recurrence is very common.
  • Cannabis plants (Cannabis sativa) has been in use for medical purposes for thousands of years. Medical Cannabis is nowadays prescribed for prevention of nausea and vomiting associated with cancer chemotherapy, and for the treatment of anorexia associated with AIDS and cancer.
  • Cannabis plants produce a group of natural chemicals called Cannabinoids, among them A9-tetrahydrocannabinol (THC), and Cannabidiol (CBD).
  • CBD A9-tetrahydrocannabinol
  • CBD Cannabidiol
  • the immune-modulatory and anti-inflammatory properties of cannabinoids, for example CBD have been shown in animal models of various inflammatory diseases including multiple sclerosis, diabetes mellitus, inflammatory bowel, and rheumatoid arthritis, inhibiting pro-inflammatory cytokine release including fNFy, TNFa, IL-ip, IL-6, IL- 17 and Th2 cytokine release including IL-4, IL-5, IL-10, IL-13.
  • CBD cannabinoid
  • compositions comprising cannabinoids, e.g., cannabidiol derivatives, which have analgesic, anti-anxiety, anti convulsive, neuroprotective, antipsychotic, and anticancer, have been described.
  • cannabinoids e.g., cannabidiol derivatives, which have analgesic, anti-anxiety, anti convulsive, neuroprotective, antipsychotic, and anticancer
  • cannabinoids e.g., CBD, THC, etc.
  • cannabinoids for treating various types of disease, e.g., inflammatory diseases
  • the state of the art does not describe or suggest the use of a specific cannabinoid, a terpene, or combinations or mixtures thereof, for prevention, reduction, or inhibition of the growth of a nasal polyp.
  • the present invention provides a composition comprising: (a) at least one cannabinoid; (b) at least one terpene; or a combination of (a) and (b), and methods of using same, such as for reducing or inhibiting the growth of nasal polyps in a subject in need thereof, such as a subject afflicted with a chronic inflammatory disease, e.g., chronic rhinosinusitis.
  • a chronic inflammatory disease e.g., chronic rhinosinusitis.
  • a method for preventing or inhibiting the growth of a nasal polyp or reducing: the volume, the mass, the number of cells, or any combination thereof in a nasal polyp in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a pharmaceutical composition comprising at least one cannabinoid, thereby preventing or inhibiting the growth of the nasal polyp or reducing: the volume, the mass, the number of cells, or any combination thereof in a nasal polyp in the subject.
  • a pharmaceutical composition comprising at least one cannabinoid and a pharmaceutically acceptable carrier, for use in the prevention or inhibition of the growth of a nasal polyp or reduction of: the volume, the mass, the number of cells, or any combination thereof in a nasal polyp in a subject in need thereof.
  • kit comprising: (a) at least one cannabinoid selected from the group consisting of: CBD, CBG, THC, CBDV, and any combination thereof; and (b) at least one glucocorticoid.
  • the at least one cannabinoid is selected from the group consisting of: cannabidiol (CBD), cannabigerol (CBG), tetrahydrocannabinol (THC), and cannabidivarin (CBDV), and any combination thereof.
  • CBD cannabidiol
  • CBG cannabigerol
  • THC tetrahydrocannabinol
  • CBDV cannabidivarin
  • the method further comprises a step of co-administering to the subject a therapeutically effective amount of a glucocorticoid.
  • the glucocorticoid is dexamethasone or functional analog thereof.
  • the glucocorticoid and the pharmaceutical compositions are coadministered sequentially or concomitantly.
  • the subject is afflicted with chronic rhinosinusitis.
  • the chronic rhinosinusitis comprises chronic rhinosinusitis with nasal polyps.
  • the pharmaceutical composition further comprises least one terpene being selected from the group consisting of: Incensole acetate, Fenchol, Linalool, Humulene, Guaiol, Pinene, Carvacrol, Eucalyptol, 6-gingerol, Glycyrrhizin acid, Geraniol, Limonene, Lycopene, Terpineol, and any combination thereof.
  • terpene being selected from the group consisting of: Incensole acetate, Fenchol, Linalool, Humulene, Guaiol, Pinene, Carvacrol, Eucalyptol, 6-gingerol, Glycyrrhizin acid, Geraniol, Limonene, Lycopene, Terpineol, and any combination thereof.
  • the administering is orally administering, nasally administering, or both.
  • the administering is contacting the nasal polyp with the pharmaceutical composition.
  • the at least one cannabinoid is selected from the group consisting of: CBD, CBG, THC, CBDV, and any combination thereof.
  • the pharmaceutical composition comprises at least two cannabinoids being selected from the group consisting of: CBD, CBG, THC, and CBDV.
  • the at least two cannabinoids are present in the pharmaceutical composition in a weight per weight ratio (w/w) ranging from 50: 1 (w/w) to 1 :50 (w/w). [026] In some embodiments, the at least two cannabinoids are present in the pharmaceutical composition in a weight per weight ratio (w/w) ranging from 25: 1 (w/w) to 1 : 10 (w/w).
  • the at least one cannabinoid is present in the pharmaceutical composition in an amount ranging from 0.1 ng to 500 mg.
  • the kit further comprises instructions for administering the at least one cannabinoid and the at least one glucocorticoid in a mole per mole (m/m) ratio ranging from 15: 1 (m/m) to 1: 1 (m/m), to a subject in need thereof.
  • the administering is sequentially administering or concomitantly administering.
  • Figs. 1A-1D include micrographs showing that nasal polyp cells/tissue pieces are starting to detach and disaggregate from the main polyp (arrows) after 3 h of cannabidiol (CBD; 150 pM) incubation.
  • Fig. 2 includes a micrograph showing the effect of 24 h of incubation of nasal polyps with CBD, dexamethasone (DX), or vehicle (VEH).
  • CBD and DX were applied at a concentration of 600 pM and 150 pM, respectively.
  • a significant detachment of nasal polyp cells/pieces was observed.
  • Fig. 3 includes a micrograph showing that 72 h post incubation of nasal polyps with CBD (150 pM), cells/tissue pieces are breaking off the main polyp into the medium. Vehicle - VEH.
  • Fig. 4 includes a vertical bar graph showing that CBD induces a significant nasal polyp weight reduction (similar to the effect of DX) after 3 h of incubation. CBD and DX were applied at a concentration of 150 pM. (Significant One-Way ANOVA with LSD post hoc, # , p ⁇ 0.002).
  • Fig. 5 includes a vertical bar graph showing that CBD induces significant dosedependent nasal polyp weight reduction after 24 h of incubation.
  • CBD was applied at concentrations of: 15 pM, 150 pM, and 1.5 mM.
  • DX was applied at a concentration of 150 pM, either alone or with 150 pM of CBD.
  • Fig. 6 includes a vertical graph showing that a mixture of CBD and reduced DX dose results in an additive effect on weight reduction of incubated nasal polyps.
  • a similar effect on nasal polyp weight reduction was observed for CBD applied alone at a concentration of 600 pM and for DX applied at a concentration of 150 pM.
  • Combining CBD (600 pM) with a low dose of DX (75 pM) resulted in an increased effect of nasal polyp weight reduction.
  • Fig. 7 includes a vertical bar graph showing that CBD significantly increased the percent of dead cells in enzymatically dissociated nasal polyps 24 h post incubation, in a dosedependent manner.
  • Human nasal polyp tissues were removed during surgery, placed in a growth medium, and incubated with: Vehicle, CBD, or DX. Twenty-four hours (24 h) post incubation a significant increase in cell death was observed in the presence of CBD (quantified by propidium iodide (Pl)-positive staining in flow cytometry).
  • CBD was applied at concentrations of: 15 pM, 150 pM, and 1.5 mM.
  • DX was applied at a concentration of 150 pM. (Significant One-Way ANOVA with LSD post hoc, # , p ⁇ 0.03).
  • Fig. 8 includes a vertical bar graph showing that CBD significantly reduces or inhibits level of granulocyte-macrophage colony-stimulating factor (GM-CSF).
  • GM-CSF granulocyte-macrophage colony-stimulating factor
  • SEB Staphylococcal Enterotoxin type B
  • CBD was applied at concentrations of: 2.5 pM, 5 pM, and 10 pM, 30 min before SEB.
  • DX was applied at a concentration of 1 pM 30 minutes before SEB. (Significant One-Way ANOVA with LSD post hoc #, p ⁇ 0.01).
  • Fig. 9 includes a vertical bar graph showing the effect of tetrahydrocannabinol (THC) on the viability of nasal polyp cells.
  • THC tetrahydrocannabinol
  • Fig. 10 includes a vertical graph showing that a mixture of CBD together with a reduced THC dose results in an additive effect on increased cell death in incubated nasal polyps, after 72 hours of incubation.
  • CBD applied alone at a concentration of 150 pM induced substantial cell death of incubated nasal polyp cells.
  • CBD (150 pM) and low dose THC (10 pM) had superior effect, resulting in an increased % of cell death (approx. 70%).
  • Fig. 11 includes a vertical bar graph showing the effect of various cannabinoids, or their combination, on T cell survival.
  • the effect on cell death in human Jurkat T cell line was examined in the presence of CBD (1 pM, 5 pM, 10 pM, and 20 pM), THC (1 pM, and 5 pM), cannabigerol (CBG; 5 pM, 10 pM, and 20 pM), cannabidivarin (CBDV; 5 pM, 10 pM, and 20 pM), and the following combinations: CBD 10 pM + CBG 5 pM; CBD 10 pM + CBG 10 pM; CBD 5 pM + CBDV 10 pM; CBD 5 pM + CBDV 20 pM; and CBD 10 pM + CBDV 10 pM. (Significant One-Way ANOVA with LSD post hoc, # ,p ⁇ 0.04).
  • Fig. 12 includes a vertical bar graph showing the effects of CBD, CBG, DX, at 10 pM doses, or combinations thereof, on cell death of dispersed nasal polyp cells.
  • Cell were incubated with cannabinoids or DX for 2 h to evaluate the fast-acting effect of the different cannabinoids, or their combinations on nasal polyp cell death.
  • the cannabinoids CBD and CBG showed a significant increase in cell death after 2 h of incubation. This effect was not observed for DX. (Significant One-Way ANOVA with LSD post hoc, # , p ⁇ 0.01, * ⁇ 0.001).
  • Figs. 13A-13D include micrographs and graph showing the effect of CBG on nasal polyp cells/tissue pieces size and cell survival.
  • 13A A micrograph showing that after 24 h of CBG (150 pM) incubation, the size of nasal polyps is reduced.
  • 13B A vertical bar graph showing % weight reduction (13A).
  • 13C A micrograph showing the effect of CBG on the viability of nasal polyp cells. CBG at a concentration of 150 pM had induced substantial cell death of incubated nasal polyp cells.
  • 13D A vertical bar graph showing % cell of NP of (13C) DETAILED DESCRIPTION
  • a method for preventing or inhibiting the growth of a nasal polyp or reducing: the volume, the mass, the number of cells, or any combination thereof in a nasal polyp in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a pharmaceutical composition comprising at least one cannabinoid, thereby preventing or inhibiting the growth of the nasal polyp or reducing: the volume, the mass, the number of cells, or any combination thereof in a nasal polyp in the subject.
  • nasal polyps encompasses any noncancerous growth within the nasal cavity and/or sinus.
  • the method comprises decolorizing a nasal polyp contacted with the composition of the invention.
  • a nasal polyp contacted with the composition disclosed herein is decolorized.
  • a subject treated according to the method disclosed herein is characterized by or comprises at least one decolorized nasal polyp.
  • At least 5%, at least 15%, at least 30%, or at least 50% of the nasal polyps in a subject treated according to the method of the invention are decolorized, or any value and range therebetween.
  • Each possibility represents a separate embodiment of the invention.
  • decolorized is compared to a control.
  • a control comprises a nasal polyp uncontacted with the composition of the invention.
  • a control comprises a nasal polyp refractory to the method of the invention.
  • a subject is afflicted with nasal polyps, per se, e.g., meaning the subject is not afflicted with another disorder or a disease related to nasal polyps.
  • “related to” refers to the nasal polyps being the causing agent inducing the development of a disorder or a disease or being the result of a disorder or a disease.
  • the subject is afflicted only with nasal polyps.
  • the subject is afflicted with nasal polyps and a background disorder or a disease.
  • the subject is at risk of developing nasal polyps.
  • the subject is afflicted with chronic rhinosinusitis.
  • chronic rhinosinusitis comprises chronic rhinosinusitis with nasal polyps.
  • “at risk” refers to increased likelihood of the subject to develop nasal polyps compared to a control.
  • a control comprises a healthy subject.
  • a control comprises a subject not afflicted with an inflammatory disease. In some embodiments, a control comprises a subject not afflicted with asthma.
  • increased comprises at least: 5%, 15%, 25%, 50%, 75%, 100%, 150%, 250%, 500%, or 1,000% increase, or any value and range therebetween.
  • increased comprises 1-20%, 15-65%, 20-120%, 75-250%, 150-500%, 350-850%, or 100- 1,000%.
  • Each possibility represents a separate embodiment of the invention.
  • reducing or inhibiting the growth of nasal polyps comprises reducing: the volume of nasal polyps, the number of cells in nasal polyps, the volume of extracellular fluid accumulated in the nasal polyps, the rate of extracellular fluid accumulation in the nasal polyps, the rate of metaplastic alteration of epithelial cells covering the nasal polyps (e.g., from respiratory epithelium to squamous epithelium), the number of squamous epithelial cells covering the nasal polyps, the area of squamous epithelium covering the nasal polyps, the ratio between the area covered by squamous epithelium to the area covered by respiratory epithelium of the nasal polyps, or any combination thereof.
  • the method comprises reducing the volume of nasal polyps. In some embodiments, the method comprises reducing the number of cells of nasal polyps. In some embodiments, the method comprises reducing or inhibiting inflammation of nasal polyps. In some embodiments, the method comprises reducing or inhibiting migration of an immune cell towards or to nasal polyps. In some embodiments, the method comprises reducing Th2 -biased eosinophilic inflammatory response. In some embodiments, the method comprises reducing Th2-biased accumulation of any immune cells involved in the inflammatory response. In some embodiments, the method comprises reducing or inhibiting sinonasal microbial colonization or virus infection.
  • the method comprises reducing or inhibiting accumulation of extracellular fluid by the nasal mucosa. In some embodiments, the method comprises reducing or inhibiting necrosis or cell death of cells of the nasal mucosa. In some embodiments, the method comprises reducing or inhibiting secretion of molecules from the nasal mucosa, wherein such molecules trigger or induce Th2 inflammatory response, immune cell recruitment, or both.
  • Th2 inflammatory response refers to a response of the immune system of an organism, which includes interleukins (IL), including but not limited to IL-4, -5, and -13, which relate to the promotion of immunoglobulin E (IgE) and eosinophilic responses in atopy, and an inflammatory response.
  • IL interleukins
  • IgE immunoglobulin E
  • Th2 inflammatory response and “type 2 inflammatory response” are interchangeable.
  • the at least one cannabinoid comprises or consists of a phytocannabinoid.
  • the at least one cannabinoid comprises or consists of an endocannabinoid, a metabolite thereof, an endocannabinoid-like molecule, or any combination thereof.
  • the term “endocannabinoid-like molecule” refers to any compound that is structurally related to a true endocannabinoid.
  • the endocannabinoid-like molecule is devoid of highly unsaturated fatty acids.
  • the endocannabinoid-like molecule does not bind to a cannabinoid receptor.
  • the endocannabinoid-like molecule binds to a cannabinoid receptor with lower binding affinity compared to an endocannabinoid or a metabolite thereof.
  • an endocannabinoid-like compound is selected from a group comprising N- acylethanolamines, or A-acylglycines. In one embodiment, an endocannabinoid-like compound is selected from a group comprising 2-monoacylglycerols.
  • Non-limiting examples of A-acylethanolamines include, but are not limited to: Anandamide, /'/-Palmitoylethanolamine, 7V-Oleoylethanolamine, A-Stearoylethanolamine, N- Docosahexaenoylethanolamine, A-Docosatetraenoylethanolamine, and A-homo-gamma- linol enoylethanolamine.
  • 2-monoacylglycerols encompasses any compound comprising a molecule of glycerol linked to a fatty acid by an ester bond, wherein the fatty acid is attached to a secondary alcohol of the glycerol molecule.
  • a “phytocannabinoid” is a cannabinoid that originates in nature from the Cannabis plant.
  • cannabinoids include, but are not limited to, cannabidiol (CBD), cannabidivarin (CBDV), (-)-A 9 -/ra//.s-tetrahydrocannabinol (A 9 -THC), (-)-A 9 -/ra//.s- tetrahydrocannabinolic acid (A 9 -THCA), (-)-A 9 -/ra//.s-tetrahydrocannabivarin (A 9 -THCV), (- )-A 9 -/ra/7.s-tetrahydrocannabivarinic acid (A 9 -THCVA), cannabinol (CBN), cannabivarin (CBNV), cannabicyclol (CBL), cannabigerol (CBG),
  • CBD cannabige
  • the present invention is directed to a composition derived from a plant extract.
  • a plant extract of the invention is derived from a plant comprising cannabinoids.
  • the plant extract of the invention is derived from a Cannabis plant.
  • the plant extract is derived from a specific species of the Cannabis genus.
  • the invention relates to a composition comprising at least one cannabinoid.
  • the at least one cannabinoid is selected from: Cannabidiol (CBD), Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabidivarin (CBDV), Cannabichromene (CBC), or any combination thereof.
  • THC comprises or is Delta 9-THC (A9-THC).
  • the at least one cannabinoid is CBD. In some embodiments, the at least one cannabinoid is CBG.
  • the composition comprises a single cannabinoid.
  • the single cannabinoid is CBD. [081] In some embodiments, the single cannabinoid is CBG.
  • the invention relates to a composition comprising a plurality of cannabinoids.
  • the composition comprises at least two cannabinoids being selected from: CBD, CBG, THC, and CBDV.
  • the composition comprises CBD and CBG. In some embodiments, the composition comprises CBD and THC. In some embodiments, the composition comprises CBD and CBDV. In some embodiments, the composition comprises CBD, CBG, and THC. In some embodiments, the composition comprises CBD, CBG, and CBDV. In some embodiments, the composition comprises CBD, THC, and CBDV. In some embodiments, the composition comprises CBD, CBG, THC, and CBDV.
  • the composition comprises CBG and THC. In some embodiments, the composition comprises CBG and CBDV. In some embodiments, the composition comprises CBG, THC, and CBDV.
  • the composition comprises CBD and CBG is a weight per weight (w/w) or mole per mole ratio (m/m) ranging from: 25:1 to 1:25, 20:1 to 1:20, 15:1 to 1:15, 10:1 to 1:10, 5:1 to 1:5, 3:1 to 1:3, or is 1:1.
  • w/w weight per weight
  • m/m mole per mole ratio
  • the composition comprises CBD and THC is a weight per weight (w/w) or mole per mole ratio (m/m) ranging from: 50:1 to 1:50, 40:1 to 10:1, 30:1 to 20:5, 25:1 to 5:1, 20:1 to 10:1, or 17:1 to 12:1.
  • w/w weight per weight
  • m/m mole per mole ratio
  • the composition comprises CBG and THC is a weight per weight (w/w) or mole per mole ratio (m/m) ranging from: 50:1 to 1:50, 40:1 to 10:1, 30:1 to 20:5, 25:1 to 5:1, 20:1 to 10:1, or 17:1 to 12:1.
  • w/w weight per weight
  • m/m mole per mole ratio
  • the composition comprises CBD and CBDV is a weight per weight (w/w) or mole per mole ratio (m/m) ranging from: 10:1 to 1:10, 9:1 to 1:9, 8:1 to 1:8, 7:1 to 1:7, 6:1 to 1:6, 5:1 to 1:5, 4:1 to 1:4, 3:1 to 1:3, 2:1 to 1:2, or is 1:1.
  • the composition comprises a combination of “high or medium CBD” and “low THC”.
  • the composition comprises a combination of “high or medium CBG” and “low THC”.
  • the composition comprises a combination of “high or medium CBG” and “low glucocorticoid”.
  • the composition comprises a combination of “high or medium CBD” and “low glucocorticoid” combination.
  • “high” , “medium”, and “low”, refer to a dose, concentration, weight, of a compound as described herein, being administered to a subject in need thereof, as disclosed herein.
  • the term “high” and “low” refers to any case wherein the w/w or m/m ratio ranges as disclosed herein.
  • “high” or “medium” is to be meant as being in the composition in a w/w or m/m ratio compared to “low” of at least: 100: 1, 90: 1, 80: 1, 70: 1, 60: 1, 50: 1, 40: 1, 30: 1, 20: 1, 10: 1, 8: 1, 6: 1, 4: 1, 3: 1, or 2: 1, or any value and range therebetween.
  • “high” or “medium” is to be meant to being in the composition in a weight or mole of at least 100-fold greater, at least 90-fold greater, at least 80-fold greater, at least 60-fold greater, at least 50-fold greater, at least 40- fold greater, at least 30-fold greater, at least 20-fold greater, at least 10-fold greater, at least - fold greater, at least 8-fold greater, at least 6-fold greater, at least 4-fold greater, or at least 2- fold greater, than the weight or mole of “low” (e.g., THC) in the composition.
  • “low” e.g., THC
  • the method comprises administering a first pharmaceutical composition comprising “high or medium CBD” and administering a second composition comprising a “low” glucocorticoid, e.g., sequentially administering, as disclosed herein.
  • the method comprises administering a first pharmaceutical composition comprising “high or medium CBG” and administering a second composition comprising a “low” glucocorticoid, sequentially administering, as disclosed herein.
  • the method comprises administering a first pharmaceutical composition comprising a “low” glucocorticoid and administering a second composition comprising, “high or medium CBD” e.g., sequentially administering, as disclosed herein.
  • the method comprises administering a first pharmaceutical composition comprising a “low” glucocorticoid and administering a second composition comprising “high or medium CBG”, e.g., sequentially administering, as disclosed herein.
  • the composition further comprises at least one terpene being selected from: Incensole acetate, Fenchol, Linalool, Humulene, Guaiol, Pinene, Carvacrol, Eucalyptol, 6-gingerol, Glycyrrhizin acid, Geraniol, Limonene, Lycopene, Terpineol, or any combination thereof.
  • terpene being selected from: Incensole acetate, Fenchol, Linalool, Humulene, Guaiol, Pinene, Carvacrol, Eucalyptol, 6-gingerol, Glycyrrhizin acid, Geraniol, Limonene, Lycopene, Terpineol, or any combination thereof.
  • the at least one cannabinoid, the at least one terpene, or both are present in the pharmaceutical composition and administered at a dose of at least: 0.1 ng/kg/day, 1 ng/kg/day, 10 ng/kg/day, 100 ng/kg/day, 1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, or 20 mg/kg/day, or any value and range therebetween.
  • 0.1 ng/kg/day 1 ng/kg/day
  • 10 ng/kg/day 100 ng/kg/day
  • 1 mg/kg/day 1 mg/kg/day
  • 5 mg/kg/day 10 mg/kg/day
  • 20 mg/kg/day or any value and range therebetween.
  • the at least one cannabinoid, the at least one terpene, or both are present in the pharmaceutical composition and administered at a dose ranging from: 0.1-100 ng/kg/day, 1-1,000 ng/kg/day, 100-1,000 ng/kg/day, 100 ng/kg/day, 10 mg/kg/day, or 1-20 mg/kg/day.
  • a dose ranging from: 0.1-100 ng/kg/day, 1-1,000 ng/kg/day, 100-1,000 ng/kg/day, 100 ng/kg/day, 10 mg/kg/day, or 1-20 mg/kg/day.
  • administering refers to any method which, in sound medical practice, delivers a composition containing an active agent to a subject in such a manner as to provide a therapeutic effect.
  • One aspect of the present subject matter provides for dermal or transdermal administration of a therapeutically effective amount of a composition of the present subject matter to a patient in need thereof.
  • Other suitable routes of administration can include oral, nasal, sublingual, buccal, or dermal.
  • administering is orally administering.
  • administering is nasally administering.
  • administering is orally administering and nasally administering.
  • administering is sublingual administering.
  • administering is sublingual administering and nasally administering. In some embodiments, administering is to the site of inflammation. In some embodiments, administering is to the site of an enlarged polyp. In some embodiments, administering is to the site of a polyp targeted for growth reduction and/or inhibition. [0105] In some embodiments, administering comprises contacting a nasal polyp with the herein disclosed composition.
  • Administering the composition to a specific site in the subject may be performed with any method known in the art. This may include an inhalator.
  • the method further comprising a step of co-administering to the subject a therapeutically effective amount of a glucocorticoid.
  • glucocorticoids suitable for human therapy are common and would be apparent to one of ordinary skill in the art.
  • the glucocorticoid is or comprises dexamethasone or functional analog thereof.
  • the term “functional analog” refers to any derivative of dexamethasone as long as it maintains at least 70%, 80%, 90%, 95%, 99%, or 100% the activity of dexamethasone in reducing: the volume of nasal polyps, the number of cells in nasal polyps, the volume of extracellular fluid accumulated in the nasal polyps, the rate of extracellular fluid accumulation in the nasal polyps, the rate of metaplastic alteration of epithelial cells covering the nasal polyps (e.g., from respiratory epithelium to squamous epithelium), the number of squamous epithelial cells covering the nasal polyps, the area of squamous epithelium covering the nasal polyps, the ratio between the area covered by squamous epithelium to the area covered by respiratory epithelium of the nasal polyps, or any combination thereof, as disclosed herein, or any value at range therebetween.
  • Each possibility represents a separate embodiment of the invention.
  • the glucocorticoid and the pharmaceutical compositions are coadministered sequentially or concomitantly.
  • the method comprises a first administering of the pharmaceutical composition comprising the at least one cannabinoid to the subject, and a second administering of the glucocorticoid to the subject.
  • the second administering comprises administering the glucocorticoid and the pharmaceutical composition of the invention.
  • the method comprises a first administering of the glucocorticoid to the subject, and a second administering of a pharmaceutical composition comprising at least one cannabinoid the to the subject.
  • the first administering comprises administering the glucocorticoid and the pharmaceutical composition of the invention.
  • a pharmaceutical composition comprising a first dose of at least one cannabinoid, e.g., CBD and/or CBG, is administered to the subject, and at a later period the glucocorticoid, e.g., dexamethasone, either solely or in conjunction with a second dose of the pharmaceutical composition as described above, is administered to the subject.
  • cannabinoid e.g., CBD and/or CBG
  • a glucocorticoid e.g., dexamethasone
  • a pharmaceutical composition comprising a first or a second dose of at least one cannabinoid, e.g., CBD and/or CBG, is administered to the subject.
  • the co-administering sequentially comprises first administering the pharmaceutical composition of the invention and a second administering comprising: (i) a glucocorticoid; or (ii) a glucocorticoid and the pharmaceutical composition of the invention.
  • the co-administering sequentially comprises first administering: (i) a glucocorticoid; or (ii) a glucocorticoid and the pharmaceutical composition of the invention, and a second administering of the pharmaceutical composition of the invention.
  • sequentially comprises at least 10 minutes apart, at least 20 minutes apart, at least 30 minutes apart, at least 45 minutes apart, at least 50 minutes apart, at least 1 hour apart, at least 2 hours apart, at least 3 hours apart, at least 5 hours apart, at least 8 hours apart, or at least 12 hours apart, or any value and range therebetween.
  • Each possibility represents a separate embodiment of the invention.
  • sequentially comprises 10-60 minutes apart, 20-90 minutes apart, 30-120 minutes apart, 1-3 hours apart, 2-6 hours apart, 3-9 hours apart, or 2-12 hours apart.
  • Each possibility represents a separate embodiment of the invention.
  • the at least one cannabinoid, the at least one terpene, or both are present in the pharmaceutical composition in an amount of at least: 0.1 ng, 0.5 ng, 1 ng, 10 ng, 50 ng, 100 ng, 500 ng, 1 mg, 2 mg, 3 mg, 4 mg, 5 mg, 10 mg, 20 mg, 50 mg, 100 mg, 500 mg, or any value and range therebetween.
  • 0.1 ng, 0.5 ng, 1 ng, 10 ng, 50 ng, 100 ng, 500 ng, 1 mg, 2 mg, 3 mg, 4 mg, 5 mg, 10 mg, 20 mg, 50 mg, 100 mg, 500 mg, or any value and range therebetween Each possibility represents a separate embodiment of the invention.
  • the at least one cannabinoid, the at least one terpene, or both are present in the pharmaceutical composition in an amount of ranging from: 0.1-100 ng, 1-1,000 ng, 100 ng-l,000 ng, lOO ng-lO mg, 1-100 mg, or 1-1,000 mg.
  • Each possibility represents a separate embodiment of the invention.
  • the subject is afflicted with or at increased risk of developing an inflammatory disease. In some embodiments, the subject is afflicted with or at increased risk of developing an IgE-related disease.
  • IgE-related disease encompasses any disorder involving immunoglobulin E as part of the disorder pathogenesis, pathophysiology, or both. In some embodiments, IgE-related disease comprises any symptom or disorder associated therewith.
  • the inflammatory disease comprises chronic rhinosinusitis.
  • the inflammatory disease comprises chronic rhinosinusitis with nasal polyps.
  • the subject is being treated with anti -chronic rhinosinusitis therapy or medication. In some embodiments, the subject is being treated with anti-chronic rhinosinusitis with nasal polyps.
  • the subject is being treated with a steroid or any medication comprising same.
  • the subject is being treated for chronic rhinosinusitis or chronic rhinosinusitis with nasal polyps with a steroid or any medication comprising same.
  • the IgE-related disease comprises asthma. In some embodiments, the IgE-related disease comprises allergy.
  • the subject is characterized by having increased serum levels of: Interleukin (IL)-4, IL-5, IL-13, high blood eosinophil cells’ number and/or high blood eosinophil cells’ number expressing eosinophil cationic protein (ECP), endocannabinoid/endocannabinoid-like/prostaglandin inflammatory profile, or any combination thereof.
  • IL Interleukin
  • IL-13 high blood eosinophil cells
  • ECP eosinophil cationic protein
  • ECP eosinophil cationic protein
  • the subject is a mammal subject, such as, but not limited to a human.
  • the subject is a human subject.
  • CRSwNP chronic rhinosinusitis with nasal polyps
  • the method comprises reducing the expression levels, serum levels, or both, of: IL-4, IL-5, IL-13, ECP, and blood eosinophil cells’ number, or any combination thereof, of the subject.
  • the method comprises reducing: cell proliferation, cell survival, cell functionally, or any combination thereof, of an immune cell, of the subject.
  • immune cell refers to any cell of the host defense system within an organism which protects against disease, pathogens, other pathological agents or abnormalities, or divergence from homeostasis.
  • the cell is a T lymphocyte. In some embodiments, the cell is an eosinophil. In some embodiments, the cell is an epithelial cell. In some embodiments, the cell is a macrophage cell. In some embodiments, the cell is a monocytic cell. In some embodiments, the cell is dendritic cell.
  • cell functionality of an eosinophil comprises eosinophil cationic protein (ECP) expression, secretion, or both.
  • ECP eosinophil cationic protein
  • expression refers to the biosynthesis of a gene product, including the transcription and/or translation of the gene product.
  • expression of a nucleic acid molecule may refer to transcription of the nucleic acid fragment (e.g., transcription resulting in mRNA or other functional RNA) and/or translation of RNA into a precursor or mature protein (polypeptide).
  • “reducing” or “reduction” is at least: 2.5%, 3%, 4%, 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% reduction, or any value and range therebetween. Each possibility represents a separate embodiment of the invention. In some embodiments, “reducing” or “reduction” is 1-20%, 5-50%, 20-90%, 60-99%, 30-75%, 15-95%, or 85-100% reduction. Each possibility represents a separate embodiment of the invention.
  • the method further comprises administering to the subject a therapeutically effective amount of a steroid.
  • the steroid comprises a low-dose steroid.
  • “low-dose steroid” is compared to a control or standard dose of steroid being administered to a subject treated solely with a steroid.
  • low-dose steroid is compared to a control or standard dose of steroid being administered to a subject not treated with the composition of the invention, not treated according to the herein disclosed method, or both.
  • the herein disclosed composition enables to reduce the therapeutic effective amount of a steroid administered to a subject in need thereof, or completely save or spare a subject from steroid therapy.
  • the subject is afflicted with asthma.
  • the method further comprises a step of selecting a subject afflicted with asthma. In some embodiments, the method further comprises a step of determining a subject is afflicted with or has increased predisposition to developing asthma.
  • a subject determined to be afflicted with asthma or having increased predisposition to developing asthma has increased suitability for treatment according to the herein disclosed method, compared to a control.
  • treatment encompasses alleviation of at least one symptom thereof, a reduction in the severity thereof, or inhibition of the progression thereof. Treatment need not mean that the disease, disorder or condition is totally cured.
  • a useful composition herein needs only to reduce the severity of a disease, disorder, or condition, reduce the severity of symptoms associated therewith, or provide improvement to a patient or subject’s quality of life.
  • alleviated symptoms of the disease, disorder or condition include reduced cell viability, inhibited cell proliferation, reduced protein expression, secretion, or both.
  • reduced protein expression, secretion, or both relates to IL-4, IL-5, IL-13, ECP, or any combination thereof.
  • Methods for determining protein expression, secretion, or both are common and would be apparent to one of ordinary skill in the art.
  • Non-limiting examples for methods of determining expression and/or secretion include, but are not limited to, RT-PCR, real time RT-PCR, next generation sequencing, western blot, dot blot, enzyme linked immunosorbent assay (ELISA), and other, some of which are exemplified hereinbelow (in the Example section).
  • RT-PCR real time RT-PCR
  • next generation sequencing western blot
  • dot blot dot blot
  • enzyme linked immunosorbent assay ELISA
  • the term “prevention” of a disease, disorder, or condition encompasses the delay, prevention, suppression, or inhibition of the onset of a disease, disorder, or condition.
  • prevention relates to a process of prophylaxis in which a subject is exposed to the presently described compositions or composition prior to the induction or onset of the disease/disorder process. This could be done where an individual has a genetic pedigree indicating a predisposition toward occurrence of the disease/disorder to be prevented. For example, this might be true of an individual whose ancestors show a predisposition toward certain types of, for example, inflammatory disorders.
  • suppression is used to describe a condition wherein the disease/disorder process has already begun but obvious symptoms of the condition have yet to be realized.
  • the cells of an individual may have the disease/disorder, but no outside signs of the disease/disorder have yet been clinically recognized.
  • prophylaxis can be applied to encompass both prevention and suppression.
  • treatment refers to the clinical application of active agents to combat an already existing condition whose clinical presentation has already been realized in a patient.
  • treating comprises ameliorating and/or preventing.
  • the subject is refractory to other nasal polyps’ treatment/therapy .
  • other nasal polyp’s treatment/therapy comprises steroid treatment/therapy .
  • refractory nasal polyps is steroid refractory nasal polyps — the steroid treatment does not treat, ameliorate or improve a clinical outcome or a clinical score in a subject afflicted with nasal polyps.
  • steroid refractory nasal polyps is characterized by deterioration in at least one nasal polyps’ symptom, or a condition associated with nasal polyps.
  • a pharmaceutical composition comprising at least one cannabinoid for use in the reduction, prevention, or inhibition of growth of nasal polyps in a subject in need thereof.
  • the subject is afflicted with or is at increased risk of developing an inflammatory disease.
  • composition comprising: (a) at least one cannabinoid; (b) at least one terpene; or (c) a combination of (a) and (b), for use in the prevention or treatment of a chronic rhinosinusitis with nasal polyps (CRSwNP), in a subject in need thereof.
  • CRMwNP chronic rhinosinusitis with nasal polyps
  • the composition further comprises a pharmaceutically acceptable carrier.
  • the composition is a pharmaceutical composition.
  • the cannabinoid is not a psychoactive cannabinoid. In one embodiment, the composition does not comprise or is devoid of a psychoactive cannabinoid. [0159] In some embodiments, the composition of the invention comprises at least 0.01%, 0.1%, 0.5%, 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99% cannabinoids by weight of the composition, or any value and range therebetween. Each possibility represents a separate embodiment of the invention.
  • the composition comprises or consists of a plant extract.
  • the at least one cannabinoid is a synthetic cannabinoid.
  • extract comprises the whole extract, a fraction thereof, a portion thereof, an isolated compound therefrom, or any combination thereof.
  • the extract is derived from a plant material.
  • the plant material is first dried and then extracted. In some embodiments, the plant material is air-dried. In some embodiments, the plant material is further heat treated (e.g., hot drying) and then extracted.
  • treatment before extraction comprises, for example, sifting, freezing, drying, lyophilizing, or any combination thereof.
  • the plant material is further processed prior to the extraction procedure in order to facilitate the extraction procedure.
  • processing methods prior to extraction include but are not limited to sifting, crushing, slicing, or shredding, such as by using a grinder or other devices to fragment the plant parts into small pieces or powder.
  • the extraction is a solvent-based extraction.
  • the solvent is a polar solvent.
  • a polar solvent may be selected from the group including, but not limited to, ethanol and Isopropyl alcohol.
  • the solvent is a non-polar solvent.
  • the extraction is a solvent-less-based extraction.
  • synthetic cannabinoid refers to a compound that has a cannabinoid, endocannabinoid, cannabinoid-like, or endocannabinoid-like structure, and is manufactured using chemical means rather than by a plant or any other type of extraction apparent to one of ordinary skill in the art.
  • carrier refers to any component of a pharmaceutical composition that is not the active agent.
  • pharmaceutically acceptable carrier refers to non-toxic, inert solid, semi-solid liquid filler, diluent, encapsulating material, formulation auxiliary of any type, or simply a sterile aqueous medium, such as saline.
  • sugars such as lactose, glucose and sucrose, starches such as com starch and potato starch, cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt, gelatin, talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, com oil and soybean oil; glycols, such as propylene glycol, polyols such as glycerin, sorbitol, mannitol and polyethylene glycol; esters such as ethyl oleate and ethyl laurate, agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline, Ringer's solution; ethyl oleate, Ringer's solution;
  • substances which can serve as a carrier herein include sugar, starch, cellulose and its derivatives, powered tragacanth, malt, gelatin, talc, stearic acid, magnesium stearate, calcium sulfate, vegetable oils, polyols, alginic acid, pyrogen-free water, isotonic saline, phosphate buffer solutions, cocoa butter (suppository base), emulsifier (e.g. carbomer, hydroxypropyl cellulose, sodium lauryl sulfate) as well as other non-toxic pharmaceutically compatible substances used in other pharmaceutical formulations.
  • sugar, starch, cellulose and its derivatives powered tragacanth, malt, gelatin, talc, stearic acid, magnesium stearate, calcium sulfate, vegetable oils, polyols, alginic acid, pyrogen-free water, isotonic saline, phosphate buffer solutions, cocoa butter (suppository base), emulsifier (
  • wetting agents and lubricants such as sodium lauryl sulfate, as well as coloring agents, flavoring agents, excipients, stabilizers, antioxidants, and preservatives may also be present.
  • Any non-toxic, inert, and effective carrier may be used to formulate the compositions contemplated herein.
  • Suitable pharmaceutically acceptable carriers, excipients, and diluents in this regard are well known to those of skill in the art, such as those described in The Merck Index, Thirteenth Edition, Budavari et al., Eds., Merck & Co., Inc., Rahway, N.J.
  • compositions examples include distilled water, physiological saline, Ringer's solution, dextrose solution, Hank's solution, and DMSO.
  • the presently described composition may also be contained in artificially created structures such as liposomes, ISCOMS, slow-releasing particles, and other vehicles which increase the half-life of the peptides or polypeptides in serum.
  • Liposomes include emulsions, foams, micelles, insoluble monolayers, liquid crystals, phospholipid dispersions, lamellar layers and the like.
  • Liposomes for use with the presently described peptides are formed from standard vesicle-forming lipids which generally include neutral and negatively charged phospholipids and a sterol, such as cholesterol. The selection of lipids is generally determined by considerations such as liposome size and stability in the blood.
  • the carrier may comprise, in total, from about 0.1% to about 99.99999% by weight of the pharmaceutical compositions presented herein.
  • a pharmaceutical composition may take any physical form necessary for proper administration.
  • the composition comprising an encapsulated one or more cannabinoid compounds can be administered in any suitable form, including but not limited to a liquid form, a gel form, a semi- liquid (e.g., a liquid, such as a viscous liquid, containing some solid) form, a semi-solid (a solid containing some liquid) form, or a solid form.
  • Compositions can be provided in, for example, a tablet form, a capsule form, a liquid form, a food form a chewable form, a non-chewable form, a transbuccal form, a sublingual form, a nasal form, a slow-release form, a non-slow-release form, a sustained release form, or a non-sustained- release form.
  • a pharmaceutically acceptable carrier suitable for the preparation of unit dosage form of a composition as described herein for peroral administration is well-known in the art.
  • compositions further comprise binders (e.g. acacia, cornstarch, gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, povidone), disintegrating agents (e.g. cornstarch, potato starch, alginic acid, silicon dioxide, croscarmellose sodium, crospovidone, guar gum, sodium starch glycolate), additives such as albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween 20, Tween 80, Pluronic F68, bile acid salts), protease inhibitors, surfactants (e.g.
  • binders e.g. acacia, cornstarch, gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, povidone
  • disintegrating agents e.g. cornstarch, potato starch, alg
  • sodium lauryl sulfate permeation enhancers
  • solubilizing agents e.g., glycerol, polyethylene glycerol
  • stabilizers e.g. hydroxypropyl cellulose, hydroxypropylmethyl cellulose
  • viscosity increasing agents e.g. carbomer, colloidal silicon dioxide, ethyl cellulose, guar gum lubricants (e.g. stearic acid, magnesium stearate, polyethylene glycol, sodium lauryl sulfate), flow-aids (e.g. colloidal silicon dioxide), plasticizers (e.g.
  • diethyl phthalate, triethyl citrate polymer coatings (e.g., poloxamers or poloxamines), and/or coating and film forming agents (e.g. ethyl cellulose, acrylates, polymethacrylates).
  • polymer coatings e.g., poloxamers or poloxamines
  • coating and film forming agents e.g. ethyl cellulose, acrylates, polymethacrylates.
  • preparation of effective amount or dose can be estimated initially from in vitro and/or ex-vivo assays.
  • a dose can be formulated in animal models and such information can be used to more accurately determine useful doses in humans.
  • toxicity and therapeutic efficacy of the active ingredients described herein can be determined by standard pharmaceutical procedures in vitro, in cell cultures, tissue culture or experimental animals.
  • the data obtained from these in vitro and/or ex-vivo and/or cell culture assays and/or animal studies can be used in formulating a range of dosage for use in human.
  • the dosages vary depending upon the dosage form employed and the route of administration utilized.
  • the exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. [See e.g., Fingl, et al., (1975) "The Pharmacological Basis of Therapeutics", Ch. 1 p.l],
  • compositions for use in the methods of this invention comprise solutions or emulsions, which in some embodiments are aqueous solutions or emulsions comprising a safe and effective amount of the cannabinoids of the present invention and optionally, other compounds as described herein, including excipients intended for topical intranasal administration.
  • the composition is administered by oral administration of a liquid preparation.
  • liquid formulations include solutions, suspensions, dispersions, emulsions, oils, aerosols, and the like.
  • the composition is administered nasally, and is thus formulated in a form suitable for nasal administration.
  • the composition is administered topically to body surfaces, and is thus formulated in a form suitable for topical administration.
  • suitable topical formulations include gels, ointments, creams, lotions, drops and the like.
  • the active ingredients disclosed herein e.g., one or more cannabinoids
  • an additional appropriate therapeutic agent or agents prepared and applied as solutions, suspensions, or emulsions in a physiologically acceptable diluent with or without a pharmaceutical carrier.
  • the composition is the form of a powder.
  • the composition is dissolved in or comprises a lipophilic liquid and/or solvent .
  • the lipophilic liquid and/or solvent comprises or is oil.
  • the oil is selected from: olive oil, sesame oil, medium chain triglyceride (MCT) oil, soy oil, sunflower oil, safflower oil, avocado oil, or any combination thereof.
  • polar liquid or solvent is based on or comprises water (an aqueous solution or solvent).
  • the composition is aseptic. In some embodiments, the composition is sterile or sterilized. In some embodiments, the composition is filtered.
  • the composition is formulated as a spray or for spraying.
  • the composition is administered nasally as a spray, a nasal wash, nasal drops, or any combination thereof.
  • the method comprises nasally administering the composition being formulated as a spray or a nasal wash.
  • the administering comprises spraying the composition of the invention to the nasal cavity of a subject in need thereof.
  • the administering comprises washing the nasal cavity of a subject in need thereof with the composition of the invention.
  • composition of the invention further comprises at least one preserving agent.
  • a composition for parenteral administration includes aqueous solution of the active preparation in water-soluble form.
  • suspensions of the active ingredients are prepared as appropriate oily or water-based injection suspensions.
  • Suitable lipophilic solvents or vehicles include, in some embodiments, fatty oils such as sesame oil, or synthetic fatty acid esters such as ethyl oleate, triglycerides or liposomes.
  • Aqueous injection suspensions contain, in some embodiments, substances, which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol or dextran.
  • the suspension also contains suitable stabilizers or agents which increase the solubility of the active ingredients to allow for the preparation of highly concentrated solutions.
  • compositions are formulated, in some embodiments, for atomization and inhalation administration. In another embodiment, compositions are contained in a container with attached atomizing means. [0189] In some embodiments, the composition is formulated for oral administration. In some embodiments, the composition is formulated for sublingual administration. In some embodiments, the composition is formulated for intranasal administration.
  • administration comprises the use of a vaporizer. In some embodiments, administration comprises the use of a humidifier. In some embodiments, administration comprises the use of an inhaler or an inhalation device.
  • a vaporizer, a humidifier, an inhalator may receive a composition in a liquid form.
  • a vaporizer, a humidifier, an inhalator may convert the composition into a vapor and/or an aerosol to be inhaled, or otherwise received by a subject.
  • a vaporizer may include a cartridge, an atomizer, and a battery.
  • the cartridge may include a reservoir to hold a liquid to be vaporized.
  • the atomizer may include a heating element to convert the liquid into a vapor and/or aerosol. Optimally, the atomizer can vaporize the liquid without initiating combustion.
  • the battery may have an electrical charge to power the atomizer and other accessories, for example, an indicator light that illuminates while the electronic cigarette is operating.
  • Vaporization provides many advantages over traditional combustion based methods of administering cannabinoids. For example, since a vaporizer can convert a composition into a vapor, the levels of each ingredient in the composition, including the cannabinoid, are controllable. Also, since vaporization does not involve combustion, a user may inhale or otherwise receive a bio-active ingredient, for example via inhalation, without being required to receive high levels of tar and various toxins associated with smoking. Vaporization also benefits from advantages such as rapid intake, direct delivery to the bloodstream, enhanced control of over- and under-dosage, and avoidance of respiratory disadvantages associated with combustion-based smoking. Vaporization may occur at approximately 180-190 °C, which may significantly reduce pyrolytic smoke compound generation. Additionally, vaporization may occur below the typical point of combustion where smoke and associated toxins are generated (e.g., 230 °C).
  • the amount of a composition to be administered will be dependent on the subject being treated, the severity of the affliction, the manner of administration, the judgment of the prescribing physician, etc. [0195] The dosage administered will be dependent upon the age, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.
  • the present invention provides combined preparations.
  • a combined preparation defines especially a “kit of parts” in the sense that the combination partners as defined above can be dosed independently or by use of different fixed combinations with distinguished amounts of the combination partners i.e., simultaneously, concurrently, separately or sequentially.
  • the parts of the kit of parts can then, e.g., be administered simultaneously or chronologically staggered, that is at different time points and with equal or different time intervals for any part of the kit of parts.
  • the ratio of the total amounts of the combination partners in some embodiments, can be administered in the combined preparation.
  • the combined preparation can be varied, e.g., in order to cope with the needs of a patient subpopulation to be treated or the needs of the single patient which different needs can be due to a particular disease, severity of a disease, age, sex, or body weight as can be readily made by a person skilled in the art.
  • kits comprising: (a) at least one cannabinoid selected from: CBD, CBG, THC, CBDV, and any combination thereof; and (b) at least one glucocorticoid.
  • the kit further comprises instructions for administering the at least one cannabinoid and the at least one glucocorticoid in a mole per mole (m/m) ratio ranging from 15: 1 (m/m) to 1: 1 (m/m), to a subject in need thereof.
  • kits comprising: (a) at least one cannabinoid selected from the group consisting of: CBD, A 9 -THC , CBG, CBDV, CBC, and any combination thereof; and (b) at least one terpene selected from the group consisting of: Incensole acetate, Fenchol, Linalool, Humulene, Guaiol, Pinene, Carvacrol, Eucalyptol, 6- gingerol, Glycyrrhizin acid, Geraniol, Limonene, Lycopene, Terpineol, and any combination thereof.
  • the kit further comprises instructions for administering the at least one cannabinoid CBD and the at least one terpene in a weight per weight (or molar) ratio ranging from 100: 1 to 1 : 100, to a subject in need thereof.
  • the at least one cannabinoid, the at least one terpene, or both are packaged within a container.
  • the container is made of a material selected from: thin-walled film or plastic (transparent or opaque), paperboard-based, foil, rigid plastic, metal (e.g., aluminum), glass, etc.
  • the content of the kit is packaged, as described below, to allow for storage of the components until they are needed.
  • kits may be packaged in suitable packaging to maintain sterility.
  • the at least one cannabinoid and the at least one terpene are stored in separate containers within the main kit containment element e.g., box or analogous structure, may or may not be an airtight container, e.g., to further preserve the sterility of some or all of the components of the kit.
  • the main kit containment element e.g., box or analogous structure
  • the airtight container e.g., to further preserve the sterility of some or all of the components of the kit.
  • the dosage amount of the at least one cannabinoid and the at least one terpene provided in a kit may be sufficient for a single application or for multiple applications.
  • the kit may have multiple dosage amounts of the at least one cannabinoid and the at least one terpene packaged in a single container, e.g., a single tube, bottle, vial, Eppendorf, and the like.
  • the kit may have multiple dosage amounts of the at least one cannabinoid and the at least one terpene antagonist individually packaged such that certain kits may have more than one container of the at least one cannabinoid and the at least one terpene.
  • multiple dosage amounts of the at least one cannabinoid and the at least one terpene may be packed in single separate containers.
  • the kit contains instructions for preparing the composition used therein and for how to practice the methods of the invention.
  • the kit further comprises a measuring utensil such as syringe, measuring spoon or a measuring cup.
  • the kit further comprises an inhalator.
  • the kit further comprises a device configured to administer the composition of the at least one cannabinoid and the at least one terpene by inhalation.
  • the instructions may be recorded on a suitable recording medium or substrate.
  • the instructions may be printed on a substrate, such as paper or plastic, etc.
  • the instructions may be present in the kit as a package insert, in the labeling of the container of the kit or components thereof (i.e., associated with the packaging or sub-packaging) etc.
  • the instructions are present as an electronic storage data file present on a suitable computer readable storage medium, e.g. CD- ROM, diskette, etc.
  • the actual instructions are not present in the kit, but means for obtaining the instructions from a remote source, e.g. via the internet, are provided.
  • An example of this embodiment is a kit that includes a web address where the instructions can be viewed and/or from which the instructions can be downloaded. As with the instructions, this means for obtaining the instructions is recorded on a suitable substrate.
  • a length of about 1,000 nanometers (nm) refers to a length of 1,000 nm ⁇ 100 nm.
  • NP nasal polyps
  • DNPCs Dispersed Nasal Polyps Cells
  • DNPCs are prepared from nasal polyps by means of enzymatic digestion.
  • Nasal Polyps are incubated for 2 hours at 37 °C in RPMI 1640 (1 g of tissue per 4 mL) containing 2.0 mg/mL protease, 1.5 mg/mL collagenase, 0.75 mg/mL hyaluronidase, and 0.05 mg/mL DNase.
  • the cell suspension is then filtered through a 70-mm cell strainer to remove any undigested tissue and washed 2 times with washing medium (RPMI 1640 supplemented with 2% FCS, 2 mmol/L glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin).
  • the cell pellet is resuspended in erythrocyte lysis buffer and washed with washing medium. After washing, DNPCs are suspended in culture medium (RPMI 1640 supplemented with 10% Foetal Calf Serum (FCS), 2 mmol/L glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin).
  • FCS Foetal Calf Serum
  • PI Propidium Iodate
  • FACS flow cytometry
  • ELISA enzymatic-linked immunoassay
  • NP samples are cut in pieces (10-90 mg tissue weight each) and incubated in RPMI medium (0-10% FCS) with different concentrations and combinations of cannabinoids, terpenes, or dexamethasone for 3 h, 24 h and 72 h. Tissue weight is recorded at time zero, and again after incubation with vehicle or treatments. In addition, cell death is quantified by PI and flow cytometry following tissue enzymatic digestion.
  • Human T cells line (Jurkat) is cultivated with RPMI medium and treated with different concentrations and combinations of cannabinoids for 2 h. T cell death is quantified by PI and flow cytometry analysis.
  • Cytokine Determination [0232] Cell cytokine production of typical Type II interleukins (IL-4, IL-5 and IL- 13) is determined in culture supernatant by ELISA.
  • PI Propidium Iodate
  • Proliferation of eosinophils and T cells is determined by labeling using the membrane proliferation marker carboxyfluorescein succinimidyl ester (CFSE).
  • CFSE carboxyfluorescein succinimidyl ester
  • Eosinophil functionality is determined based on the secretion profile of the eosinophil cationic protein (ECP). ECP levels are assessed in IL-33 activated-DNPCs supernatant by ELISA test, according to manufacture instructions.
  • PB Peripheral blood
  • PBMCs Peripheral blood mononuclear cells
  • Peripheral blood serum is collected for analysis of endocannabinoids, endocannabinoid-like molecules, prostaglandins, and cytokines.
  • control dexamethasone
  • cannabinoids terpenes, or their combination
  • Dilutions are prepared Dulbecco’s Phosphate Buffered Saline minus Ca 2+ minus Mg 2+ (Biological Industries, Beit Haemek, Israel - Catalog No 02-023-1 A - Lot # 2104085). Patients
  • Patients can present concomitant diagnosis of asthma for a period of at least 6 months prior to screening.
  • Peripheral blood eosinophils level > 300 cells/pL.
  • CBD induces detachment of human nasal polyp cells/tissues ex-vivo
  • NP human nasal polyp
  • the inventors showed that after a 24 h incubation of human NP tissues with CBD (600 pM), DX (150 pM), or vehicle - CBD (600 pM) caused significant detachment of cells/tissues from the main NP whole tissue (Fig. 2).
  • CBD induced significant human NP tissue weight reduction ex-vivo, in a dose-dependent manner (150pM to 1.5 mM; Fig. 5).
  • the effect of DX at 150pM was comparable to CBD 150 pM.
  • No additive or synergistic effect was observed when CBD and DX were co-administered at a molar ratio of 1 : 1.
  • the inventors showed a similar trend of NP weight reduction for CBG (at 150 pM; 13A- 13B)
  • the inventors examined whether an additive effect could be achieved for different molar ratios of CBD to DX. Specifically, the inventors have shown that a high dose CBD (600 pM) co-administered with a low dose DX (75 pM) provides an additive inhibitory effect on human NP tissue growth or size, ex-vivo (Fig. 6).
  • the inventors conclude that a combination of a high-dose cannabinoid, e.g., CBD, with low-dose DX, either provided concomitantly, or sequentially, can provide the desired additive inhibitory effect on human NP tissue growth or size.
  • a high-dose cannabinoid e.g., CBD
  • DX low-dose DX
  • the cannabinoid may be administered first, thus providing a fast-acting activity, which is further complemented by a later administration of DX, hence achieving the desired additive inhibitory effect on human NP tissue growth or size.
  • Cannabinoids, glucocorticoid, or their combination induces cell death of dissociated human NP cells
  • CBD 150 pM
  • THC 10 pM
  • CBG at 150 pM
  • NP cells 13C-13D
  • a combination of a first cannabinoid, e.g., CBD, with a second cannabinoid at a low-dose, e.g., THC, can provide the desired synergistic effect of promoting cell death, thus inhibiting or reducing human NP tissue growth or size.
  • the cannabinoids may be administered concomitantly or sequentially.
  • the inventors have examined the effects of CBD, CBG, DX, or combinations thereof at a concentration of 10 pM, on cell death of dispersed NP cells.
  • Cell were incubated with: (i) CBD; (ii) CBG; (iii) DX; (iv) CBD+DX; (v) CBG+DX; and (vi) CBD+CBG, for 2 h to evaluate the fast acting effect of the compounds (e.g., cannabinoid, and glucocorticoid) or their combinations on NP cell death.
  • the cannabinoids CBD and CBG induced a significant increase in cell death % after 2 h of incubation (Fig. 12).
  • the inventors have further examined the effect of various cannabinoids on the survival of T cells, which are known to be involved in chronic rhinosinusitis with nasal polyposis.
  • the inventors showed that both CBD and CBG had induced a significant cell death compared to control (vehicle; Fig. 11). Further, CBDV was also shown to induce cell death, when provided at a concentration of 20 pM.
  • SEB increases Granulocyte-macrophage colony-stimulating factor (GM-CSF) in dispersed nasal polyp cells.
  • GM-CSF is known to stimulate stem cells to produce granulocytes.
  • CBD, (like DX) was shown to significantly decrease this glycoprotein at different doses (Fig. 8), thus inhibiting granulocyte proliferation. This may reduce the number of accumulating cells in the NP during infection.

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