Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
  • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
  • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01C—PLANTING; SOWING; FERTILISING
  • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
  • A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
  • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
  • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
  • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
  • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
  • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
  • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
  • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
  • A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms

Definitions

• the invention relates to a seed treatment, and more specifically to a combination of active ingredients for treating seeds before their germination, with a view to conferring on them, as well as on the plants which will result from them, improved properties.
• pre-germination treatment Treatments of seeds after their harvest and before the start of the germination process, called pre-germination treatment, have proven to be effective in improving germination performance, in particular for seeds to be optimized with a view to their subsequent use or for seeds with germination defects.
• a seed priming treatment is known, which generally consists in soaking seeds, or seeds, in a controlled manner, in order to initiate the preliminary phase of germination, then in drying the seeds, this dehydration resulting in the blocking of the development process, and this, just before the exit of the radicle which characterizes germination.
• the seeds are then marketed, this phase involving the storage of the seeds thus treated under very variable conditions, and their germination can resume its cycle as soon as they are sown and rehydrated.
• priming methods make it possible to significantly increase the speed of germination, to improve the homogeneity of emergence of young seedlings after sowing the seeds thus treated and to increase the tolerance of plants to abiotic stresses, such as climatic hazards. . Although inducing a significant increase in germination vigour, which is particularly beneficial in market gardening sectors, they do not make it possible to stimulate the mechanisms of resistance of plants to biotic stresses. In addition, priming has the disadvantage of significantly affecting the life of the seeds thus treated, preventing carryovers of seed batches from one year to another.
• this medium can be supplemented with various active ingredients to apply chemical, osmotic, hormonal or other type ingredients to the seeds.
• active ingredients are chosen without limitation from germination activators, in particular nitrate and phosphate salts; osmotic agents such as polyethylene glycol, salts such as sodium and potassium chlorides, polyols; growth regulators; growth hormones; hormone regulators.
• the imbibition medium can also be exposed to physical treatments such as ultraviolet or gamma radiation or else to plasma treatments.
• these primings do not have little or no favorable impact on plant resistance to biotic stresses; they also have an unfavorable effect on the lifespan of the seeds. This is an important parameter, a loss of vigor and viability of the seeds during their storage which can lead to the loss of considerable volumes of seeds for marketing.
• the present invention provides a solution combining the advantages of the priming methods of the prior art and the resolution of the problems encountered with the known methods.
• the invention provides a kit for preparing an imbibition medium for a seed priming treatment, said kit comprising and being configured for a combined application of at least two different agents, said agents being chosen from: a) one or more germination activators for said seeds, the concentration of the or each of said activators in the imbibition medium being 0.001-10 mM, b) one or more agents capable of providing protection for said seeds and/or plants which are derived therefrom, the concentration in the imbibition medium of the or each of said agents being 0.01-10 mM, and c) one or more regulators of the cellular oxidative mechanisms of said seeds.
• agents being chosen from: a) one or more germination activators for said seeds, the concentration of the or each of said activators in the imbibition medium being 0.001-10 mM, b) one or more agents capable of providing protection for said seeds and/or plants which are derived therefrom, the concentration in the imbibition medium of the or each of said agents being 0.01-10 mM, and c
• seed and grain are used to define the stage of development of the plant before germination but also covering the beginnings of germination, and the term “plant” to define any stage of development of a plant commencing with the rupture of the seed coat and the elongation of the radicle.
• a treatment which acts on the embryo, the albumen and/or on the envelope(s), directly or indirectly, this action being able to have an effect on the seedling and/or the plant that comes from it.
• Such an action makes it possible to control the physiology of the embryo to promote its growth during the germination and post-germination phases, to weaken the seed coats, to increase the adaptation of the seed and/or that of the plant to vis-à-vis unfavorable physical, chemical and/or biological conditions, such as biotic stress such as aggression by pathogenic organisms, or abiotic stress such as osmotic stress or thermal stress.
• This treatment comprises comprising at least one step of soaking the seeds and one step of drying said seeds thus treated.
• This treatment can be carried out by any technique provided that it exerts an effect, direct or indirect, on the embryo, the albumen and/or on the envelope(s).
• the imbibition of the seeds can be carried out by soaking in a solution for a determined period and environment, or by any other appropriate imbibition technique, the seeds thus soaked then being dried.
• Such a definition thus excludes a surface treatment of the seeds by coating or filming with a protective agent, this type of treatment not acting on the embryo, nor on the albumen and the envelopes.
• germination activator or agent a) is meant an agent which will trigger and/or stimulate the germination conditions of a batch of seeds which, in the absence of such an activator, would have germinated less and /or germinated less quickly and heterogeneously; thus, it can act by direct or indirect signaling by promoting the synthesis of molecules necessary for germination and/or promoting the catabolism of molecules that inhibit germination
• agent capable of ensuring protection of said seeds and/or plants resulting therefrom or agent b) according to the invention is meant an agent which will trigger and/or stimulate the natural defenses of seeds and young plants which, in the absence of such an agent, would not have been activated or activated to a lesser extent; thus, it can promote the adaptation of seeds or plants to a wide spectrum of pathogens or pests.
• regulator of the cellular oxidative mechanisms of said seeds or agent c) is meant an agent which will trigger and/or stimulate the cellular detoxification mechanisms of seeds or young plants which, in the absence of such a regulator , would not have been activated or less activated; thus, it can promote the conservation of treated seeds and/or the germination of seeds under environmental conditions that are unfavorable for the species.
• the invention is described below in detail and its implementation variants exposed.
• the representative molecules may be of different chemical natures, their function as defined above possibly resulting from different biological mechanisms. However, it is important to specify that these functions are known to those skilled in the art and that by using their general knowledge, they are able to choose one or more molecules as agents a), b) and c) , respectively. Molecules representative of each of these agents are given in the description, it being understood that the invention is not restricted thereto. It is effectively the combination of such agents according to the invention which can bring about, in addition to the benefits of the priming, an improvement in the physiological properties of adaptation of the plants resulting from these seeds or of the seeds themselves.
• the drying step in the context of a treatment according to the invention can be carried out by any means allowing the treated seed to return to substantially the same state of dehydration as before the treatment. Thus, it can be carried out in the absence or under a flow of air or of another inert gas, at ambient temperature or at a temperature compatible with the seed, that is to say not affecting its properties. It is part of the general knowledge of those skilled in the art to determine, depending on the type of seed, the drying conditions, as well as to measure the water or humidity content of a seed, for example by means of a moisture meter. .
• kit according to the invention it is understood that the agents a), b) and/or c) are each placed in solid or flexible containers, for example of the ampoule or pouch type. They are in liquid or solid form, directly usable in a treatment according to the invention or requiring dilution or solubilization. This the latter is carried out in any solvent compatible with the imbibition of the seeds, water being preferred.
• a kit of the invention comprises at least two different agents chosen respectively from: a) one or more germination activators of said seeds, the concentration of the or each of said activators in the imbibition medium being 0.001-10 mM, b) one or more agents capable of providing protection for said seeds and/or the plants resulting therefrom, the concentration of the or each of said agents in the imbibition medium being 0.1-10 mM, and c) one or more regulators of the cellular oxidative mechanisms of said seeds.
• a kit of the invention is in particular dedicated to the treatment of seeds intended to be stored before being sown.
• it comprises: a) one or more germination activators of said seeds, the concentration of the or each of said activators in the imbibition medium being 0.01-10 mM, and at least one of the two agents b) and c) following: b) one or more agents capable of ensuring protection of the said seeds and/or of the plants resulting therefrom, the concentration of the or each of the said agents in the imbibition medium being 0.1 -10 mM, c) one or more regulators of the cellular oxidative mechanisms of said seeds.
• it comprises three different agents chosen respectively from germination activators a) in a concentration as indicated above, agents b) in a concentration as indicated above, and regulators c) .
• the germination activator(s) a) can be chosen from dormancy inhibitors. In particular, they may be abscisic acid catabolism activators and/or hormone activators such as gibberellins. Thus, a germination activator a) can be chosen from stimulators, precursors and donors of nitric oxide production, such as arginine, citrulline, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), as well as mixtures thereof.
• stimulators, precursors and donors of nitric oxide production such as arginine, citrulline, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), as well as mixtures thereof.
• a kit of the invention comprises one or more germination activators a) chosen from nitrates, nitrites, arginine, citrulline, sodium nitroprusside (SNP), S-nitrosoglutathione (GSNO), phosphates, sulfates, ethylene, ethylene precursors, ammonium salts, allantoin, and mixtures thereof.
• germination activators a) chosen from nitrates, nitrites, arginine, citrulline, sodium nitroprusside (SNP), S-nitrosoglutathione (GSNO), phosphates, sulfates, ethylene, ethylene precursors, ammonium salts, allantoin, and mixtures thereof.
• Nitrates, nitrites, phosphates and sulfates, and any mixture thereof may each be present in a concentration in the imbibition medium of 0.1 to 10 mM, alone or in combination.
• the agent or agents a) are chosen from potassium nitrate at a concentration in the imbibition medium of 0.1-10 mM, calcium nitrate at a concentration in the medium of imbibition of 0.1-10 mM, magnesium sulfate at a concentration in the imbibition medium of 0.1-10 mM, monobasic potassium phosphate at a concentration in the imbibition medium of 0.1-10 mM, potassium hydrogen phosphate at a concentration in the imbibition medium of 0.1-10 mM, gibberellic acid at a concentration in the imbibition medium of 0.01-1 mM and sodium nitroprusside at a concentration in the imbibition medium of 0.01-1 mM.
• the agent(s) capable of providing protection for said seeds and/or the plants resulting from them b) may be chosen from stimulators of the natural defenses of seeds and stimulators of the natural defenses of plants resulting from said seeds, in response to a biotic stress and/or abiotic stress.
• Preferred agents b) are selected from molecules of the salicylates family, such as salicylic acid at a concentration in the imbibition medium of 0.05-3 mM, derivatives of salicylic acid, in particular esters, and acibenzolar-S-methyl.
• Preferred salicylic acid derivatives are sulfosalicylic acid and methyl salicylate when present in the imbibition medium at a concentration of 0.1-5 mM.
• the regulator or regulators of cellular oxidative mechanisms c) can be chosen from osmoprotectors and antioxidants.
• Preferred osmoprotectants are chosen from proline present in a concentration in the imbibition medium of 0.1-30 mM, trehalose, aminobutyric acid, reducing sugars, as well as mixtures thereof.
• Preferred antioxidants are selected from ascorbic acid and its salts, such as its alkali metal and alkaline earth metal salts, in a concentration in the imbibition medium of 0.05-150 mM, glutathione reduced to a concentration in the imbibition medium of 0.01-5 mM, tocopherols in a concentration in the imbibition medium of 0.05-30 mM and tocopherol acetate in a concentration in the imbibition medium of 0 .05-30 mM.
• ascorbic acid and its salts such as its alkali metal and alkaline earth metal salts
• the invention further relates to a seed treatment method comprising the following steps: the seeds are soaked in an imbibition medium obtainable from a kit as defined above and in particular in any of its variants, until saturation, then the seeds thus soaked are dehydrated.
• the imbibition phase corresponds to absorption of the imbibition medium by the seed.
• the amount of water or medium that a seed must absorb during this imbibition phase is a concept well known to those skilled in the art and depends on the seed. Generally, it is preferred that the seed absorb at least 75% by weight of the amount of water or imbibition medium required by the seed to enter the germination stage (Phase II of the germination process).
• the invention also relates to the use of a kit as defined above, to carry out a seed priming treatment.
• a seed priming treatment These are generally intended to be stored before being sown, but of course they can be used directly after a priming treatment according to the invention.
• This comprises at least one step of soaking the seeds with at least one aqueous solution of the agents a), b) and/or c) defined in the kit and is followed by a step of drying or dehydration.
• This imbibition can be carried out by any means and in particular by impregnation or spraying with one or more liquid solutions of said agents a), b) and/or c).
• dried seeds are obtained which have undergone a priming treatment which can be stored for several months, and which have remarkable germination properties.
• kits of the invention also lies in the fact that it is effective on most seeds and in particular the seeds of cultivated or cultivable plant species.
• vegetable seeds, aromatic seeds, floral seeds and field seeds can be treated with the prospect of activating germination but also of strengthening the unborn plant, in particular with regard to biotic and abiotic stresses.
• FIG. 1 represents a comparison of the germination kinetics of untreated and treated tomato seeds according to the invention in Example 1.
• FIG. 2 represents a comparison of the kinetics of germination of untreated and treated mint seeds according to the invention in Example 2.
• FIG. 3 represents a comparison of the germination kinetics of untreated and treated tomato seeds according to the invention in Example 3.
• FIG. 4 represents a comparison of the germination kinetics of lettuce seeds that are untreated and treated according to a treatment outside the invention illustrated in example 4, by way of comparison.
• FIG. 5 represents a comparison of the germination kinetics of untreated and treated lettuce seeds according to the invention in Example 5.
• FIG. 6 represents a comparison of the germination kinetics of untreated and treated lettuce seeds according to the invention in Example 6.
• FIG. 7 represents a comparison of the germination kinetics of tomato seeds treated according to the invention in Example 7 and treated by a treatment outside the invention (hydro-priming) for comparison, after 18 months of storage.
• FIG. 8 represents a comparison of the germination capacity of seeds 7 days after the germination of lettuce seeds treated according to the invention in Example 8 and treated by a treatment outside the invention (hydro-priming) for comparison, after a controlled deterioration treatment.
• KNO3 potassium nitrate
• Ca(NOs)2 calcium nitrate
• MgSO4 magnesium sulphate
• KH2PO4 monobasic potassium phosphate
• K2HPO4 potassium hydrogen phosphate
• Each example 1-6 illustrates the implementation of a kit according to the invention in a seed priming treatment comprising imbibition and drying, then the germination of the seeds thus treated, and the comparison with seeds not processed.
• Example 7 illustrates the implementation of a kit according to the invention in a seed priming treatment comprising imbibition and drying, their storage for a period of 18 months and then the germination of the seeds as well treated, and the comparison with seeds treated by hydro-priming (excluding invention) in an imbibition medium consisting only of water and having undergone the same storage conditions.
• Example 8 illustrates the implementation of a kit according to the invention in a seed priming treatment comprising imbibition and drying, their controlled deterioration to assess their storage performance and then the germination of the seeds thus treated, and the comparison in seeds treated by hydro-priming (outside the invention) in an imbibition medium consisting only of water and having undergone the same conditions of controlled deterioration.
• the general treatment protocol followed in the examples consists in preparing the imbibition medium by dissolving the agents a), b) and/or c) of a kit of the invention in water and allowing the seeds for a period of 4h to 336h at temperatures between 4 and 50°C.
• the seeds are then dried under a constant air flow for a period of 2h to 96h in temperatures between 4 and 50°C and a relative humidity between 15% and 90%.
• the germination protocol for the seeds, whether treated or not, is the same for all the examples. 100 seeds are germinated on a filter paper of the AnchorBlue type (10 ⁇ 16 cm) soaked to saturation with reverse osmosis water. Germination tests are conducted for each condition with 4 replicates, representing at least 400 seeds tested per condition. The germination rate is determined by the ratio of the number of germinated seeds to the number of total seeds.
• This step is carried out with an aqueous solution comprising at least two of the agents a), b) and c) as specified in each of the examples.
• the duration of the treatment is greater than 4 hours; for tomatoes, it is generally 104 hours.
• the temperature is maintained between 2°C and 40°C.
• the seeds After soaking in the aqueous solution, the seeds are rinsed with water and then dried under a stream of air at ambient temperature for 3 days.
• the seeds thus dried are stored, before/after treatment, at a temperature below 10°C.
• the protocol for the controlled deterioration of seeds treated according to a process of the invention or treated by hydro-priming is composed of three phases and is carried out on re-dried treated seeds.
• the first phase called the equilibration phase, consists in putting the treated seeds dry at a temperature of 20°C and a relative humidity of 76% for 3 days.
• the second phase called the deterioration phase, consists of transferring the seeds that have undergone the first phase to a temperature of 40°C and a relative humidity of 76% for 0, 2, 3 or 6 days.
• the third phase called the rebalancing phase, consists of transferring the seeds that have undergone the second phase to a temperature of 20°C and a relative humidity of 32% for 3 days.
• the resulting seeds are stored at a temperature of 10°C and then germinated.
• the seeds annotated “tO” correspond to seeds having only undergone the equilibration and re-equilibration phase of the controlled deterioration protocol.
• the seeds annotated “t2”, “t3” and “t6” correspond to seeds having undergone respectively 2, 3 and 6 days of deterioration phase.
• Example 1 Use of a kit according to the invention for a priming treatment of tomato seeds, comprising agents a) and c)
• the imbibition medium is an aqueous solution of the following germination activating agents a) 0.5-5 mM KNO3 + 0.1-5 mM MgSO4 + 0.1-5 mM NH4SO4 + 0.1- 5 mM KH2PO4, and the following cellular oxidative metabolism regulators c) 1-50 mM ASA + 1-5 mM GSH + 1-20 mM Pro.
• the seeds thus pretreated and untreated seeds are germinated according to the protocol described above carried out at 25° C. in continuous light.
• Example 2 Use of a kit according to the invention for a priming treatment of mint seeds, comprising agents a) and c)
• the imbibition medium is a solution of the following germination activating agents a) 0.5-5 mM KNO3 + 0.1-5 mM MgSO4 + 0.1-5 mM NH4SO4 + 0.1-5 mM KH2PO4 + 0 0.05-0.5 mM GA4 + 0.05-0.5 mM SNP, and the next regulator of cellular oxidative metabolisms, 1-20 mM Pro.
• the seeds thus pretreated and untreated seeds are germinated according to the protocol described above conducted at 25° C. in the dark.
• Example 3 Use of a kit according to the invention for a priming treatment of tomato seeds, comprising agents a), b) and c)
• the imbibition medium is a solution of the following germination-activating agents a), 0.5-5 mM KNO 3 + 0.1-5 mM MgSO 4 + 0.1-5 mM NH 4 SO 4 + 0.1 -5 mM KH 2 PO 4 , of the agent capable of ensuring protection of the seeds or of the plants resulting therefrom b) according to 0.1-5 mM SA, and of the regulators of cellular oxidative metabolisms c) according to 1-50 mM ASA + 1-5 mM GSH + 1-20 mM Pro.
• Example 4 Use of a kit for a priming treatment of lettuce seeds, comprising agents a)
• the imbibition medium is an aqueous solution of the following germination activators a) 0.5-5 mM KNO 3 + 0.1-5 mM MgSO 4 + 0.1-5 mM NH 4 SO 4 + 0.1-5 mM KH 2 PO 4 .
• Example 5 Use of a kit according to the invention for a priming treatment of lettuce seeds, comprising agents a) and b)
• the imbibition medium is an aqueous solution of the following germination activators a) 0.5-5 mM KNO 3 + 0.1-5 mM MgSO 4 + 0.1-5 mM NH 4 SO 4 + 0.1- 5 mM KH 2 PO 4 , and of the agent capable of ensuring protection of the seeds or of the plants resulting therefrom b), 0.1-5 mM SA.
• Example 6 Use of a kit according to the invention for a priming treatment of lettuce seeds, comprising agents a) and c)
• the imbibition medium is an aqueous solution of the following germination activators a) 0.5-5 mM KNO 3 + 0.1-5 mM MgSO 4 + 0.1-5 mM NH 4 SO 4 + 0.1- 5 mM KH 2 PO 4 , and regulators of cellular oxidative mechanisms c) following 1- 50 mM ASA+ 1-5 mM GSH.
• Example 7 Use of a kit according to the invention for a priming treatment of tomato seeds, comprising agents a) and c)
• the imbibition medium is an aqueous solution of the following germination activators a) 0.5-5 mM KNO3 + 0.1-5 mM MgSO4 + 0.1-5 mM NH4SO4 + 0.1- 5 mM KH2PO4, and regulators of the following cellular oxidative mechanisms c) 1-50 mM ASA + 1-5 mM GSH + 1-20 mM Pro.
• Example 8 Use of a kit according to the invention for a priming treatment of lettuce seeds, comprising agents a), b) and c)
• the imbibition medium is an aqueous solution of the following germination activators a) 0.5-5 mM KNO3 + 0.5-5 mM Ca(NOs)2 + 0.1-5 mM MgSO4 + 0.1- 5 mM NH4SO4 + 0.1-5 mM KH2PO4, of the agent capable of ensuring protection of the seeds or of the plants resulting therefrom b) according to 0.1-5 mM SA, and of the regulators of the cellular oxidative mechanisms c) following 1-50 mM ASA + 1-5 mM GSH.
• the germinative capacities 7 days after the germination of lettuce seeds treated by hydro-priming (grey) and treated by smart priming according to the invention (black) is represented in FIG. 8.
• the germinative capacities correspond to those of seeds treated by hydro-priming and treated by smart priming according to the invention and having undergone a controlled deterioration protocol.
• the results present the germination capacities of seeds deteriorated for 0 days (tO), 2 days (t2), 3 days (t3) or 6 days (t6).

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• Life Sciences & Earth Sciences (AREA)
• Environmental Sciences (AREA)
• Health & Medical Sciences (AREA)
• Dentistry (AREA)
• Agronomy & Crop Science (AREA)
• Pest Control & Pesticides (AREA)
• Plant Pathology (AREA)
• Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Wood Science & Technology (AREA)
• Zoology (AREA)
• Soil Sciences (AREA)
• Physiology (AREA)
• Pretreatment Of Seeds And Plants (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)

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• 2021
  • 2021-08-06 EP EP21759108.0A patent/EP4192223A1/fr active Pending
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  • 2021-08-06 CA CA3187659A patent/CA3187659A1/fr active Pending
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