EP4165396A1 - Unité de détection, procédé de mesure et procédé de fabrication - Google Patents

Unité de détection, procédé de mesure et procédé de fabrication

Info

Publication number
EP4165396A1
EP4165396A1 EP21739432.9A EP21739432A EP4165396A1 EP 4165396 A1 EP4165396 A1 EP 4165396A1 EP 21739432 A EP21739432 A EP 21739432A EP 4165396 A1 EP4165396 A1 EP 4165396A1
Authority
EP
European Patent Office
Prior art keywords
sensor unit
sensor
sensor element
membrane
reservoir
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP21739432.9A
Other languages
German (de)
English (en)
Other versions
EP4165396B1 (fr
EP4165396C0 (fr
Inventor
Daniel Riechers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Presens Precision Sensing GmbH
Original Assignee
Presens Precision Sensing GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Presens Precision Sensing GmbH filed Critical Presens Precision Sensing GmbH
Publication of EP4165396A1 publication Critical patent/EP4165396A1/fr
Application granted granted Critical
Publication of EP4165396B1 publication Critical patent/EP4165396B1/fr
Publication of EP4165396C0 publication Critical patent/EP4165396C0/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6434—Optrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/775—Indicator and selective membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7796—Special mountings, packaging of indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/80—Indicating pH value

Definitions

  • the invention relates to a sensor unit for measuring an analyte in a sample, a corresponding measuring method using the sensor unit and a method for producing the sensor unit.
  • Such sensors contain a sensor substance which is sensitive to an analyte of the sample, that is to say a substance to be detected in the sample.
  • the sensor substance can have an optical behavior which is influenced in a direct or indirect manner by the analyte. From the evaluation of the optical behavior, conclusions can be drawn quantitatively about the analyte, that is to say, for example, depending on the type of analyte, the concentration or partial pressure of the analyte can be determined.
  • Such sensors, their structure, sensor substances suitable for the respective analytes and conditions of use, and measurement methods for evaluating the optical behavior of the sensor or the sensor substance are known to the person skilled in the art, for example from the prior art mentioned above.
  • the European patent application EP 0354895 A2 relates to a single-use measuring element in which, prior to a measurement, a sensor is arranged in a measuring area of a measuring channel that is closed on both sides.
  • the measuring channel is filled with a calibration and storage medium.
  • the calibration and storage medium is displaced by a sample flowing into the measurement channel.
  • the sensor can be an optical sensor.
  • the measurement channel can be closed by a pierceable membrane.
  • the European patent application EP 0460343 A2 proposes the storage medium first to be displaced by a separating medium, which can be a calibration medium, and then to displacing the separating medium by the sample.
  • German utility model DE 202010006207 U1 relates to various arrangements with which it is possible in a measuring arrangement to at least temporarily separate an area containing a sensor from a sample area.
  • the purpose is to protect the sensor during sterilization.
  • the sensor is surrounded by an aqueous reducing agent.
  • US patent application US 2012/0267518 A1 discloses a sensor which is arranged in a storage area for protection during sterilization and can be moved from the storage area into a sample area.
  • the object of the invention is to provide a sensor unit in which the effort for moistening the sensor in advance of a measurement is eliminated.
  • Claim 12 relates to a corresponding sample vessel, claim 14 to a corresponding measuring method and claim 15 to a manufacturing method for a sensor unit.
  • the subclaims each relate to advantageous embodiments.
  • the sensor unit according to the invention comprises a sensor element which has an optical behavior that depends on at least one analyte of a sample, as well as a reservoir with an auxiliary medium.
  • a membrane is provided which, in a preparatory state of the sensor unit, enables diffusive contact between the auxiliary medium and the sensor element.
  • the sensor unit By removing the sensor element, on the one hand, and the membrane and the reservoir, on the other hand, from one another, the sensor unit can be put into a measuring state. In the measuring state of the sensor unit, the sensor unit is ready to measure the analyte. In the preparatory state of the sensor unit, the sensor unit can be stored; It is sometimes also possible to carry out procedures in advance of a measurement, for example sterilizing the sensor unit or a sample container in which the sensor unit is located.
  • the optical behavior of the sensor element which depends on the at least one analyte, results for example from the fact that the sensor element contains a sensor substance of the type mentioned at the beginning.
  • the sensor substance can be embedded in a carrier or applied to the surface of a carrier; the carrier can for example consist of a polymer.
  • the optical behavior of the sensor substance can be luminescence, for example.
  • the sensor substance can be a luminescent dye, and an intensity of the luminescent light, a decay period of the intensity of the luminescent light, a polarization of the luminescent light, a decay period of the polarization of the luminescent light or another parameter of the luminescent light can depend directly or indirectly on the analyte. Intensity, decay times, polarization or other parameters of the luminescent light can be measured using known methods.
  • Another example of the optical behavior of a sensor substance or the sensor element is the color; ie the color of the sensor element can depend directly or indirectly on the analyte.
  • the color can be measured by known methods.
  • analytes include oxygen, carbon dioxide, pH.
  • the sensor unit according to the invention has hardly any effects on a measurement with the sensor unit. This is because the reservoir, which may be contaminated with microorganisms, is removed from the sensor element before the measurement.
  • the reservoir and membrane are detachably connected to the sensor unit. The sensor element, on the one hand, and membrane and reservoir, on the other hand, can easily be removed from one another by detaching the reservoir and membrane from the sensor unit, for example by pulling them off.
  • a cap which covers the reservoir and the sensor element.
  • This cap protects the sensor unit during storage and also during sterilization of a sample vessel containing the sensor unit.
  • the sensor element is arranged on a carrier; the cap is releasably connected to the carrier, and the membrane is fastened in the cap and, together with the cap, delimits the reservoir.
  • the reservoir and membrane, together with the cap can simply be pulled off the carrier and thus removed from the sensor element.
  • the sensor unit has a sensor part and a reservoir part different therefrom.
  • the sensor element is attached to the sensor part, the reservoir is formed in the reservoir part and the membrane is attached.
  • the reservoir part and the sensor part can be rotated and / or displaced relative to one another.
  • the sensor element, on the one hand, and the membrane and reservoir, on the other hand, can be removed from one another by a corresponding rotation or displacement.
  • the sensor part has a channel in which a light guide can be guided.
  • the light guide serves to guide light to the sensor element and / or to guide light away from the sensor element.
  • the reservoir part has an opening through which the sensor element is accessible from the surroundings of the sensor unit in the measuring state of the sensor unit. Via this opening, the sensor element can in particular come into contact with a sample in which an analyte is to be measured.
  • the auxiliary medium is a storage medium in order to store the sensor element in a moisturized state in the sensor unit.
  • the storage medium can for example Be water or contain a lye, such as caustic soda.
  • a lye can be used, for example, to neutralize acids that can arise when the sensor unit is irradiated. Irradiation of the sensor unit may be necessary in the course of sterilization, but the acids formed in the process can damage the sensor element; the neutralization of these acids ensures the functionality of the sensor element despite irradiation.
  • the storage medium can also contain an antimicrobial, preferably non-volatile, preservative such as benzethonium chloride.
  • Such a preservative suppresses the growth of microorganisms in the reservoir and thus avoids falsification of measurement results as a result of the presence of these microorganisms, for example due to their metabolism, even more reliably than, as explained above, is already given by the structure of the sensor unit alone.
  • the storage medium is used to establish a defined state for calibrating the sensor element.
  • the sensor unit is intended to measure the partial pressure of CO2
  • a phosphate buffer with a few millimoles of bicarbonate and an exactly adjusted pH value can be used as a storage medium.
  • Such a buffer is radiation-stable and develops a reproducible CO 2 partial pressure. This can be used for a one-point calibration of the sensor element.
  • a sample vessel according to the invention has a sensor unit according to the invention as described above.
  • the shape of the sample vessel is not restricted. It can be a disposable or a reusable sample container. Beakers, bags, bioreactors and flow elements may be mentioned as an incomplete list of examples.
  • the sample vessel can be sterilized. Since the sensor element is protected from irradiation or irradiation products in the preparation state, the sample vessel with the attached sensor unit can be sterilized by irradiation without impairing the sensor element of the sensor unit.
  • the protection of the sensor element in the sensor unit also makes it possible, during the production of the sample vessel, to take less account of the influence on the sensor element by materials of the sample vessel or by auxiliary substances such as about to take adhesives.
  • the sensor element in the sensor unit is protected, for example from evaporation of the materials and auxiliary substances. When measuring, such vapors are displaced by the sample.
  • a method according to the invention for measuring an analyte in a sample comprises at least the following steps:
  • a sample vessel is provided with a sensor unit according to the invention as described above.
  • the sample vessel is then sterilized, for example by irradiation.
  • the sensor unit is still in the preparatory state, so the sensor element is still protected.
  • the sensor unit is then put into the measuring state by removing the sensor element on the one hand and the reservoir and membrane on the other hand from one another. This makes the sensor element accessible from the sample vessel.
  • the sample vessel is also filled with the sample. It depends on the details of the measurement carried out, which is evident to the person skilled in the art, as to whether the sensor unit is first put into the measuring state or the sample is first filled into the sample vessel; sometimes the order of these two steps is arbitrary.
  • the measurement of the analyte is carried out using the sensor element of the sensor unit according to a known method.
  • a method for producing a sensor unit comprises at least the following steps: A cavity is produced in a precut film. A sensor element of the type discussed above, which has an optical behavior dependent on an analyte, is attached to a transparent carrier. The cavity is filled with an auxiliary medium. A membrane through which the auxiliary medium or components thereof can diffuse is placed on the filled cavity and attached to the film. Finally, the carrier with the sensor element is placed on the membrane; this is done so that the sensor element faces the membrane. The carrier is attached to the foil.
  • FIG. 1 shows an embodiment of a sensor unit according to the invention in a sectional view.
  • FIG. 2 shows the embodiment of the sensor unit according to the invention from FIG. 1 in a top view.
  • FIG. 3 shows a further embodiment of a sensor unit according to the invention in a sectional view in the preparatory state.
  • FIG. 4 shows the embodiment from FIG. 3 in a sectional view in the measuring state.
  • FIG. 5 illustrates the preparation for a measurement using a sensor unit according to the invention as shown in FIGS. 1 and 2.
  • FIG. 1 shows an embodiment of a sensor unit 1 according to the invention in a sectional view.
  • a sensor element 2 is attached to a transparent carrier 3, in the example shown with a double-sided adhesive tape 21.
  • the sensor element 2 is in diffusive contact with a filling medium 51 located in a reservoir 5 via a membrane 4.
  • the reservoir 5 is delimited by a cap 6, which is formed here as a cavity in a film 61.
  • the film 61 ends in a handle 62, which is only partially shown here.
  • the membrane 4 is attached to the film 61 by a welding ring 41. Furthermore, the film 61 is fastened to the carrier 3 via a welding ring 31.
  • the sensor unit 1 is in the preparatory state.
  • the sensor element 2 is protected by the cap 6, in particular when irradiated for the purpose of
  • the diffusive contact between the auxiliary medium 51 and the sensor element 2 provided by the membrane 4 ensures that the sensor element 2 is moistened in the preparatory state, in which the sensor unit 1 can, for example, be stored.
  • the foil 61 can be an aluminum foil or a plastic-aluminum composite foil; Low-density polyethylene (LDPE), for example, is suitable as a plastic.
  • the carrier 3 can for example be made of a cycloolefin copolymer (COC).
  • the membrane 4 can be, for example, microporous polypropylene (PP).
  • the transparent carrier 3 allows light to be guided through the carrier 3 to the sensor element 2 and / or light from the sensor element 2 to be detected through the carrier 3 for the purpose of evaluating the optical behavior of the sensor element 2.
  • transparent here means that the carrier 3 is permeable to the relevant light wavelengths to such an extent that the analyte can be measured with a desired accuracy.
  • the adhesive tape 21 must then also be transparent.
  • the membrane 4 is attached to the film 61 by means of a welding ring 41. It would also be conceivable, for example, to fasten the membrane 4 by means of a clamping ring, in which case the clamping ring is fastened to the film 61, e.g. by means of a welding ring.
  • FIG. 2 shows a top view of the sensor unit 1 shown in FIG. 1.
  • the handle 62 is shown in its entirety here.
  • the sensor element 2 is indicated by a circle. 1, the cap 6, including the reservoir 5 and membrane 4, can be removed from the carrier 3 with the handle 62, the connection provided by the welding ring 31 being released, more precisely the welding ring 31 being released from the carrier 3 and remaining on the foil 61, while the connection given by the welding ring 41 remains; the sensor element 2 is then accessible from outside the sensor unit 1.
  • the sensor unit 1 is thus put into the measuring state.
  • the sensor unit 1 shows a sectional view of a further embodiment of the sensor unit 1 according to the invention.
  • the sensor unit 1 is shown in the preparatory state.
  • the sensor unit 1 comprises a sensor part 20 to which the sensor element 2 is attached, here by means of a carrier 3.
  • the sensor unit 1 furthermore comprises a reservoir part 50 in which the reservoir 5 is formed.
  • the reservoir 5 is delimited on one side by the membrane 4, which is also attached to the reservoir part 50, otherwise by a metallized film 52; the film 52 acts as a diffusion barrier.
  • a channel 22 for a light guide is formed in the sensor part 20.
  • a light guide can be brought up to the carrier 3 via the channel 22 in order to guide light through the light guide and via the carrier 3 to the sensor element 2 and / or to guide light away from the sensor element 2.
  • the reservoir part 50 also has an opening 54 through which contact between a sample and the sensor element 2 is possible in the measurement state. In the preparatory state shown, there is diffusive contact between the sensor element 2 and the reservoir 5 through the membrane 4, so that the auxiliary medium (not shown here) in the reservoir 5 or components thereof can reach the sensor element 2 by diffusion through the membrane 4.
  • Sensor part 20 and reservoir part 50 can be rotated relative to one another about an axis 200; By such a rotation, the sensor unit 1 can be put into the measurement state shown in FIG. 4. Sensor part 20 and reservoir part 50 are sealed from one another by O-rings 53. Flange 56 on reservoir part 50 is used to attach sensor unit 1 to a sample vessel. Depending on the configuration of the sample vessel and the sensor unit 1, this attachment can take place in different ways. If, for example, the sample vessel is a plastic bag and the flange 56 is also made of plastic, the bag and flange can be welded or glued. If the sample vessel has a solid wall, the flange can be connected to the wall, for example, by a screw connection or a bayonet lock. In the embodiment shown, a retaining clip 70 ensures that the reservoir part 50 and sensor part 20 are held together.
  • FIG. 4 shows the sensor unit 1 from FIG. 3 in the measuring state. All of the elements shown have already been discussed in relation to FIG. 3. Here, a sample can now reach the sensor element 2 through the opening 54. In contrast, there is no longer any diffusive contact between the reservoir 5 and the sensor element 2.
  • FIG. 5 shows a sequence of three stages A, B, C before a measurement with a sensor unit 1 according to the invention.
  • the sensor unit 1 which corresponds to an embodiment shown in FIGS Sample vessel 100 stuck on.
  • cap 6 covers Sensor element and reservoir (not shown here, see FIGS. 1 and 2), and the handle 62 is angled and guided out of the sample vessel 100.
  • the sample vessel 100 together with the sensor unit 1 can be irradiated for the purpose of sterilization; the sensor element is protected by the cap 6.
  • the sample vessel 100 together with the sensor unit 1 can also be stored in this way.
  • Stage B is pulled off the cap 6 including the reservoir and membrane (see Figures 1 and 2) by a force 65 on the handle 62 from the sensor unit 1, one arrives at stage C.
  • the sensor element 2 attached to the carrier 3 is from The inside of the sample vessel 100 is accessible.
  • the sample vessel 100 can now be filled with a sample. The measurement can then be carried out using the sensor element 2.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Dans une unité de détection (1), un élément de détection (2) qui présente un comportement optique qui dépend d'au moins un analyte, est en contact par diffusion, au moyen d'une membrane (4), avec un milieu auxiliaire (51) qui est présent dans un réservoir (5) dans l'unité de détection (1). Le réservoir (5) et la membrane (4) d'une part et l'élément de détection (2) d'autre part peuvent être éloignés l'un de l'autre pour amener l'unité de détection (1) dans un état de mesure. Le milieu auxiliaire (51) peut servir au mouillage de l'élément de détection (2) lors du stockage de l'unité de détection (1) ou également à l'étalonnage de l'élément de détection (2).
EP21739432.9A 2020-06-16 2021-06-07 Unité de détection, procédé de mesure et procédé de fabrication Active EP4165396B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102020115791.6A DE102020115791A1 (de) 2020-06-16 2020-06-16 Sensoreinheit, messverfahren und herstellungsverfahren
PCT/IB2021/054959 WO2021255574A1 (fr) 2020-06-16 2021-06-07 Unité de détection, procédé de mesure et procédé de fabrication

Publications (3)

Publication Number Publication Date
EP4165396A1 true EP4165396A1 (fr) 2023-04-19
EP4165396B1 EP4165396B1 (fr) 2024-04-24
EP4165396C0 EP4165396C0 (fr) 2024-04-24

Family

ID=76829583

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21739432.9A Active EP4165396B1 (fr) 2020-06-16 2021-06-07 Unité de détection, procédé de mesure et procédé de fabrication

Country Status (5)

Country Link
US (1) US20230119514A1 (fr)
EP (1) EP4165396B1 (fr)
CN (1) CN115715364A (fr)
DE (1) DE102020115791A1 (fr)
WO (1) WO2021255574A1 (fr)

Family Cites Families (15)

* Cited by examiner, † Cited by third party
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US4739645A (en) * 1986-10-17 1988-04-26 Kelsius Inc. Apparatus for calibrating a sensor for detecting the presence of a gas in a liquid
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US20230119514A1 (en) 2023-04-20
EP4165396C0 (fr) 2024-04-24
WO2021255574A1 (fr) 2021-12-23
CN115715364A (zh) 2023-02-24
DE102020115791A1 (de) 2021-12-16

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