EP3997339A1 - Dispositif de commande et procédé de commande d'une pompe à membrane exempte de capteurs - Google Patents

Dispositif de commande et procédé de commande d'une pompe à membrane exempte de capteurs

Info

Publication number
EP3997339A1
EP3997339A1 EP20731145.7A EP20731145A EP3997339A1 EP 3997339 A1 EP3997339 A1 EP 3997339A1 EP 20731145 A EP20731145 A EP 20731145A EP 3997339 A1 EP3997339 A1 EP 3997339A1
Authority
EP
European Patent Office
Prior art keywords
control fluid
control
sensor
diaphragm pump
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20731145.7A
Other languages
German (de)
English (en)
Inventor
Matthias Arnold
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP3997339A1 publication Critical patent/EP3997339A1/fr
Pending legal-status Critical Current

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
    • F04B43/06—Pumps having fluid drive
    • F04B43/073—Pumps having fluid drive the actuating fluid being controlled by at least one valve
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/0009—Special features
    • F04B43/0081—Special features systems, control, safety measures
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
    • F04B43/06—Pumps having fluid drive
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
    • F04B49/06—Control using electricity
    • F04B49/065—Control using electricity and making use of computers
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
    • F04B49/22—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00 by means of valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B2205/00—Fluid parameters
    • F04B2205/09—Flow through the pump
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B2205/00—Fluid parameters
    • F04B2205/18—Pressure in a control cylinder/piston unit

Definitions

  • the invention relates to a control arrangement for controlling a sensor-free diaphragm pump.
  • the invention also relates to a method for controlling a sensor-free diaphragm pump.
  • the invention also relates to a
  • Diaphragm pump arrangement with a diaphragm pump and a control arrangement for controlling the diaphragm pump also relates to a diaphragm pump and a bioreactor with a diaphragm pump arrangement or a diaphragm pump and the use of a diaphragm pump arrangement with a bioreactor or a diaphragm pump in a bioreactor.
  • diaphragm pumps are used to convey media.
  • Media to be conveyed in bioprocess engineering are, in particular, fluids in which, for example, biological cells and / or other components can be contained that can be the subject of studies or further use.
  • a membrane pump and its use in bioreactors is described, for example, in EP 2 379 889 B1.
  • a control arrangement and a method for controlling a diaphragm pump and a diaphragm pump which improve process reliability and / or represent a particularly cost-effective solution.
  • a control arrangement for controlling a sensor-free diaphragm pump comprising a control fluid inlet for connection to a first control fluid reservoir with a pressure level that is higher than a reference pressure, a control fluid outlet for connection to a second control fluid reservoir with a pressure level that is reduced compared to a reference pressure, a control fluid connection for connecting a control fluid line for the sensor-free diaphragm pump, the control fluid inlet being connected to a collecting point via a first proportional valve and the control fluid outlet being connected to the collecting point via a second proportional valve, a mass flow sensor being arranged between the collecting point and the control fluid connection, and wherein the control arrangement further comprises a control unit which, for signaling purposes, communicates with the first proportional valve, the second proportional valve and the mass flow sensor is bound.
  • the invention is based, inter alia, on the knowledge that existing diaphragm pumps and control arrangements as well as methods rely on a distance sensor for determining the position of the diaphragm. On the one hand, this leads to an increase in the price of the diaphragm pump itself. On the other hand, this solution is subject to inaccuracies, since the displaced volume is only determined indirectly by means of the distance sensor and the spatial deformation of the elastic diaphragm cannot be described reproducibly. In addition, the activation of the membrane and the conveying of the medium to be conveyed often result in high loads on the media components.
  • the control arrangement described here makes it possible to dispense with a distance sensor for determining the position of the diaphragm of the diaphragm pump and at the same time ensure precise and gentle conveyance of the medium to be conveyed.
  • this makes the use of sensor-free and thus significantly more cost-effective diaphragm pumps - also as disposable diaphragm pumps in disposable bioreactors.
  • it is ensured that the medium conveyed with the diaphragm pump and its components are conveyed volumetrically precisely and so gently that the process reliability is increased and further examination and further processing are improved.
  • a sensor-free diaphragm pump is understood here to mean, in particular, a diaphragm pump that does not have a distance sensor for detecting the position of the diaphragm.
  • a sensor-free membrane pump also preferably does not contain any further sensors.
  • Such designs of a diaphragm pump have the advantage that the costs for a distance sensor for detecting the position of the diaphragm and, if necessary, for further sensors can be saved, and the diaphragm pump can be manufactured significantly more cost-effectively and is therefore also available for single-use applications.
  • control arrangement described here is designed so that it can control such a sensor-free diaphragm pump.
  • control arrangement comprises the elements described below.
  • a control fluid inlet and a control fluid outlet are used to connect the
  • Control arrangement to a first and second control fluid reservoir, the control fluid inlet being connected to a first control fluid reservoir with a pressure level that is higher than a reference pressure and the control fluid outlet being connected to a second control fluid reservoir with a pressure level that is reduced compared to a reference pressure.
  • control arrangement can be connected to a sensor-free diaphragm pump by means of a control fluid line via a control fluid connection.
  • control fluid from the first control fluid reservoir can pass from the control fluid inlet to the control fluid connection and from there via the control fluid line into the control fluid-side chamber of the sensor-free diaphragm pump and from there via the
  • the first and second control fluid reservoirs can also be part of an overall control fluid reservoir, which provides the increased or reduced pressure levels required for conveying the control fluid in and out of the control fluid-side chamber of the diaphragm pump via the control arrangement compared to a reference pressure.
  • Both the control fluid inlet and the control fluid outlet are connected to a collection point, which in turn is connected to the control fluid connection.
  • a first proportional valve is located between the control fluid inlet and the collection point and a second proportional valve is located between the control fluid outlet and the collection point.
  • the control arrangement can control the control fluid flow from the control fluid inlet to the control fluid connection and from the control fluid connection to the control fluid outlet via these proportional valves.
  • the control arrangement has a control unit which is connected in terms of signaling to the first proportional valve and the second proportional valve.
  • the control arrangement also has a mass flow sensor which is arranged between the collection point and the control fluid connection.
  • the mass flow sensor is preferably designed as a thermal mass flow sensor.
  • the mass flow of the control fluid between the collection point and the control fluid connection can be determined via the mass flow sensor.
  • the control unit of the control arrangement is also connected to the mass flow sensor for signaling purposes. In this way it is possible to use signals from the mass flow sensor in the control of the first and / or second proportional valve.
  • the control fluid inlet is preferably connected to the collection point via a control fluid line
  • the control fluid outlet is likewise preferably connected to the collection point via a control fluid line.
  • the collection point is also preferably connected to the control fluid connection via a control fluid line.
  • a control fluid line which connects the control arrangement to the sensor-free diaphragm pump, can also be connected to the control fluid connection.
  • a control fluid line can preferably also be connected to the control fluid inlet and / or to the control fluid outlet in order to connect the control fluid inlet or the control fluid outlet to the first or second control fluid reservoir.
  • the sensor-free membrane pump has a chamber on the control fluid side and a chamber on the media side.
  • the control fluid is supplied to or removed from the control fluid-side chamber by the control arrangement via a control fluid line.
  • the medium to be conveyed flows through the media-side chamber.
  • An elastic membrane fluidly separates the two chambers from one another, but couples them volumetrically.
  • the media-side chamber has one or more media connections via which the medium to be conveyed flows into and out of the media-side chamber.
  • the connections can preferably be provided with a valve, for example with an automatically acting or with a controlled valve, in particular with a check valve.
  • the media-side chamber of the sensor-free membrane has exactly one media inlet for the inflow and exactly one media outlet for the outflow of the medium.
  • the media inlet and the media outlet preferably each have a check valve, in particular an automatically acting check valve, which is arranged according to the direction of flow through the respective media inlet or media outlet.
  • a bioreactor is understood here in particular as a flexible or dimensionally stable container that forms a reaction space in its interior in which a bioprocess can take place.
  • a media mixture in the bioreactor, which can also be referred to as culture broth.
  • Bioreactors often have one or more connections via which media can be added and removed, samples can be taken or sensors can be connected for various measurements.
  • Diaphragm pumps are used in bioreactors, for example, in order to be able to remove or supply media or media samples or to move media and thereby mix them, for example.
  • Bioreactors usually have to be provided sterile for bioprocesses. Since the sterilization of already used bioreactors is complex and expensive and always represents a process risk, single-use bioreactors are increasingly being used which are only intended for single use and are disposed of after use. On the one hand, this requires inexpensive constructions and materials, at the same time materials that are as resource-efficient and / or environmentally friendly as possible, but which at the same time meet the requirements of bioprocess engineering, for example the United States Pharmacopeia (USP) Class VI. In this context, disposable diaphragm pumps are also preferred, which can form part of a disposable bioreactor and after use can also be disposed of, if necessary together with the single-use bioreactor. For this, too, inexpensive, resource- and environmentally friendly materials are preferred, which at the same time meet the high requirements for bioprocess security.
  • USP United States Pharmacopeia
  • the volume flow of the control fluid can be determined via the mass flow of the control fluid detected with the mass flow sensor, which correlates with the molar mass flow. By integrating over time, the molar number of particles and the corresponding conveyed volume of the control fluid can be determined. In this way, volumetric control of the sensor-free diaphragm pump with the control arrangement described here is made possible.
  • the signaling connection of the mass flow sensor on the one hand and the first and second proportional valve on the other hand to the control unit of the control arrangement also makes it possible to control the control fluid flow from the control fluid inlet to the control fluid connection and back to the control fluid outlet according to the data recorded with the mass flow sensor and thereby the absence of a To compensate for the distance sensor for detecting the position of the membrane.
  • control arrangement described here makes it possible through its structure and the provision of the mass flow sensor between the collection point and the control fluid connection to combine all the elements required for controlling the sensor-free diaphragm pump in the control arrangement. Only simple connections to the control fluid reservoirs on the one hand and to the sensor-free diaphragm pump on the other hand are required. Furthermore, all control-relevant sensors and other components are combined in the control arrangement in this way.
  • the preferably reusable control arrangement can contain all complex and possibly expensive components and be arranged separately, possibly also spatially spaced from, for example, a disposable bioreactor with a sensor-free disposable membrane pump used therein.
  • the control arrangement is preferably designed to control more than one sensor-free diaphragm pump.
  • control fluid lines or a branching control fluid line can be connected to the control fluid connection to form several sensor-free diaphragm pumps.
  • the multiple sensorless diaphragm pumps would preferably all be the same be controlled. This is particularly suitable for parallel bioprocesses that run in parallel in a large number of identically structured arrangements.
  • the control arrangement can also preferably be designed to control several diaphragm pumps differently.
  • the components required in each case are provided correspondingly multiple times in the control arrangement.
  • the corresponding components for the control of several diaphragm pumps, also for different applications with different controls can be bundled and made available centrally in a common control arrangement.
  • the control arrangement can also, in particular if it is designed to control several diaphragm pumps in different control types, be distributed, for example also spatially spaced apart, and can be a coherent control arrangement through appropriate connections (for example signaling and / or fluid technology and / or wired and / or wired) form.
  • the mass flow sensor is designed for a measurement in both flow directions.
  • the mass flow sensor is thus preferably designed to detect the mass flow of the control fluid between the collection point and the control fluid connection, regardless of the direction of flow of the control fluid, i.e. regardless of whether the control fluid flows from the control fluid inlet to the control fluid connection or flows from the control fluid connection to the control fluid outlet.
  • a pressure sensor in particular an absolute pressure sensor, is arranged between the collecting point and the control fluid connection.
  • the pressure sensor is arranged between the mass flow rate sensor and the control fluid connection.
  • the control unit is connected to the pressure sensor for signaling purposes.
  • Providing a pressure sensor, and in particular its signaling connection with the control unit has the advantage that the control fluid pressure can also be taken into account in the control of the sensor-free diaphragm pump.
  • the volumetric coupling of the control fluid-side chamber and the media-side chamber of the sensor-free diaphragm pump not only the volume flow and correspondingly the volume of the medium to be conveyed is correlated with the control fluid, but also the pressure of the medium to be conveyed with the pressure of the control fluid.
  • the medium to be conveyed in particular its components, such as biological cells, are often pressure-sensitive and / or can be negatively influenced or even destroyed by shear forces, it is advantageous to be able to take the pressure into account in the control, and in particular limit values, pressure ranges, for example and / or pressure gradients, in particular time-related gradients, and to be adhered to in the control in order to achieve a particularly gentle conveyance of the medium to be conveyed through the sensor-free diaphragm pump.
  • the accuracy of the control of the sensor-free diaphragm pump can be further improved by including the control fluid pressure.
  • control arrangement further comprises a temperature sensor and / or a temperature sensor interface for a
  • the control unit is preferably connected to the temperature sensor and / or the temperature sensor interface for signaling purposes.
  • the temperature sensor can be designed, for example, to detect the ambient temperature.
  • the temperature sensor can be designed, for example, to detect the temperature of the medium to be conveyed, for example in a bioreactor.
  • the detection of the temperature or the use of a signal from a temperature sensor helps to further improve the control.
  • the accuracy of the control can in particular be further improved.
  • control unit has a communication interface for an exchange with an external communication unit.
  • Communication interface comprises or is a data interface for an exchange of data.
  • control algorithms, limit values, ranges, or gradients, for example for the pressure, flow velocities, mass or volume flows or volumes to be maintained can be transmitted and thus specified via the communication interface and / or values and / or evaluations generated in the control can be sent to a external communication unit are transferred and processed there.
  • control unit is designed to control a first proportional valve of a control arrangement for releasing flow and conveying a control fluid, for closing the first proportional valve when a volume to be conveyed is reached, for activating the second proportional valve for releasing flow and conveying the control fluid, and for Closing the second proportional valve when the volume to be conveyed is reached.
  • control unit is preferably designed to determine the volume of the control fluid delivered, the determination of the volume of the control fluid delivered preferably comprising: receiving a mass flow signal from the mass flow sensor, deriving a volume flow from the mass flow signal, deriving a volume of the control fluid delivered the volume flow.
  • control unit is preferably designed to determine end positions of the membrane of the sensor-free membrane pump, wherein the determination of end positions of the membrane of the sensor-free membrane pump preferably comprises: controlling the first and / or second proportional valve for conveying control fluid with a predetermined initialization volume flow, receiving a Pressure gradient signal, determining the presence of an end position of the membrane when the pressure gradient signal changes.
  • the object mentioned at the beginning is achieved by a diaphragm pump arrangement with a diaphragm pump, in particular a sensor-free diaphragm pump, and a control arrangement described above.
  • the object mentioned at the beginning is achieved by a diaphragm pump for use with a control arrangement described above characterized in that the diaphragm pump is free of a distance sensor for detecting the position of the diaphragm.
  • the above-mentioned object is achieved by a bioreactor with a previously described membrane pump arrangement or with a previously described membrane pump.
  • the object mentioned at the outset is achieved by using a previously described membrane pump arrangement with a bioreactor or a previously described membrane pump in a bioreactor.
  • the above-mentioned object is achieved by a method for controlling a sensor-free diaphragm pump, the method comprising: controlling a first proportional valve of a control arrangement for releasing flow and conveying a control fluid, closing the first proportional valve when a volume to be conveyed is reached, controlling the second Proportional valve for releasing the flow and conveying the control fluid, closing the second proportional valve when the volume to be conveyed is reached.
  • the method described here enables volumetric control of a sensor-free diaphragm pump.
  • the control fluid is initially conveyed from the first control fluid reservoir to the control fluid connection by appropriate activation of a first proportional valve, preferably with a predetermined mass flow and / or a predetermined volume flow.
  • a first proportional valve preferably with a predetermined mass flow and / or a predetermined volume flow.
  • the first proportional valve is closed.
  • the conveyed volume of the control fluid is preferably determined and compared with the desired volume to be conveyed.
  • the second proportional valve After reaching the volume to be conveyed and closing the first proportional valve, the second proportional valve is activated to release the flow and the control fluid is conveyed, preferably with a predetermined mass flow and / or with a predetermined volume flow, from the control fluid connection to the second control fluid reservoir. After the volume to be conveyed has been reached, the second proportional valve is closed.
  • the delivered volume of the control fluid is preferably determined and compared with the desired volume to be delivered.
  • the delivery of the control fluid through the first proportional valve and delivery of the control fluid through the second proportional valve take place in different ways Flow directions, in the first case towards the diaphragm pump and in the second case away from the diaphragm pump towards the second control fluid reservoir.
  • the determination of the conveyed volume of the control fluid preferably comprises recording a mass flow of the control fluid, deriving a volume flow from the mass flow, deriving a volume of the conveyed control fluid from the volume flow.
  • the mass flow of the control fluid is preferably recorded with the mass flow sensor of the control arrangement before the control fluid leaves the control fluid connection in the direction of the sensor-free diaphragm pump.
  • approximately isothermal processes can be assumed at low speeds and differential pressures or adiabatic processes at faster changes, depending on the framework conditions.
  • the volume flow F of a gaseous control fluid can be determined, for example, on the basis of the law of ideal gases or on the basis of a functional relationship that is individually adapted to the gaseous fluid used.
  • the molar mass flow n and, if appropriate, a recorded pressure p and / or a recorded temperature T and the general gas constant R are taken into account.
  • F (n * R * T) / p.
  • the molar number of particles is preferably determined from the mass flow, which correlates with the molar mass flow, by integration over time, from which then with the help of the general gas law or a functional relationship individually adapted to the gaseous fluid and, if necessary, taking other into account Parameters such as pressure and / or temperature, the volume of the control fluid that has been conveyed through the control fluid connection can be determined.
  • the volume conveyed into the control fluid-side chamber of the diaphragm pump can in turn be derived from this.
  • a dead volume is preferably taken into account, which extends between a control fluid inlet of the control fluid-side chamber of the sensor-free membrane and the proportional valves of the control arrangement and includes all the volumes of the control fluid-carrying components in between.
  • the method further comprises detecting a pressure of the control fluid, activating the first and / or the second proportional valve in such a way that a predetermined target pressure of the control fluid is not exceeded and / or a predetermined target pressure range is complied with and / or a specified pressure gradient is maintained.
  • the pressure of the control fluid is preferably determined with a pressure sensor of the control arrangement, in particular within the control arrangement before the control fluid leaves the control fluid connection in the direction of the sensor-free diaphragm pump.
  • the sensor-free membrane pump is preferably controlled in such a way that predetermined pressure parameters are maintained.
  • a pressure gradient in particular with regard to a pressure change over time, must be observed in order to enable the medium to be conveyed as gently as possible for cells.
  • Another preferred development of the method comprises the determination of end positions of the membrane of the sensor-free membrane pump.
  • the volumetric end positions of the membrane of the sensor-free membrane pump are preferably recorded here. These volumetric end positions of the membrane correspond in particular to a maximum or minimum filling volume of the control fluid-side chamber and a maximum or minimum filling volume of the media-side chamber of the sensor-free membrane pump.
  • Determining the length of the membrane pump along the membrane of the sensor-free membrane pump preferably includes conveying control fluid with a predetermined initialization volume flow, detecting the pressure gradient of the control fluid, determining the presence of an end position of the membrane when the pressure gradient changes.
  • control fluid in particular with a predetermined initialization volume flow, is preferably conveyed and the pressure gradient of the control fluid is detected in parallel until it is established that the pressure gradient changes significantly, in particular increases. From this it can be concluded that the membrane has reached one of its end positions.
  • a clear change in the pressure gradient can also be used to determine the presence of the second end position of the membrane.
  • the total differential volume of the sensor-free diaphragm pump can be derived. Accordingly, membrane reference points and associated delivery volumes can be determined between the two end positions.
  • the neutral position of the membrane for example, corresponds to half the total differential volume.
  • control arrangement described here and its further developments have features which make them particularly suitable for being used with a method described here and its further developments or are designed accordingly.
  • Figure 1 a schematic representation of a diaphragm pump arrangement with a
  • FIG. 2 a schematic flow diagram of a method for controlling a sensor-free diaphragm pump.
  • 1 shows a schematic illustration of a diaphragm pump arrangement 100 with a control arrangement 13 and a sensor-free diaphragm pump 14, and
  • FIG. 2 shows a schematic flow chart of a method 1000 for controlling a sensor-free diaphragm pump 14.
  • FIG. 1 shows a diaphragm pump arrangement 100 with a sensor-free diaphragm pump 14 and a control arrangement 13.
  • a first and a second control fluid reservoir 1A, 2A, a bioreactor 40 and a sample container 41 are shown.
  • the sensor-free diaphragm pump 14 has an elastic diaphragm 15 which fluidly separates a chamber 16 on the control fluid side from a chamber 17 on the media side, but couples it volumetrically. Via a control fluid inlet 18 of the diaphragm pump 14, control fluid, which is provided by the control arrangement 13 via the control fluid line 10, can flow into the control fluid-side chamber 16 of the diaphragm pump 14 and flow out therefrom.
  • the media-side chamber 17 of the diaphragm pump 14 has a media inlet 20 with an automatically acting check valve 22 and a media outlet 19 likewise with an automatically acting check valve 21. Via this media inlet and outlet 20, 19, the medium to be conveyed can be fed from a bioreactor 40 to the membrane pump 14 and from the membrane pump 14 to a sample container 41, for example.
  • the sensor-free membrane pump 14 does not have a distance sensor for determining the position of the membrane 15.
  • the sensor-free membrane pump 14 also preferably has no further sensors. This has the advantage that the sensor-free diaphragm pump 14 can be produced in a cost-effective manner and can therefore also be used as a disposable diaphragm pump, in particular together with a disposable bioreactor.
  • the control fluid required for operating the diaphragm pump 14 is provided via the control fluid reservoirs 1A, 2A.
  • the first control fluid reservoir 1A has a pressure level that is higher than a reference pressure, for example a compressed air supply in the range from 200 to 10,000 hPa.
  • the second control fluid reservoir has a pressure level that is reduced compared to a reference pressure, for example in the form of a vacuum system in the range from -200 to -900 hPa.
  • the control arrangement 13 preferably contains all of the components required for the, in particular volumetric, control of the sensor-free diaphragm pump 14.
  • the control intelligence contained in the control arrangement and the components required for it can be arranged separately from the sensor-free diaphragm pump, in particular also spatially separate from it, so that, for example, the sensor arrangement 13 can be designed to be reusable and the sensor-free diaphragm pump 14 can be designed as single-use components can.
  • the control arrangement 13 has a control fluid inlet 1 for connection to the first control fluid reservoir 1A and a control fluid outlet 2 for connection to the second control fluid reservoir 2A.
  • the control fluid inlet 1 and the control fluid outlet 2 are connected to the collecting point 6 via control fluid lines.
  • the collecting point 6 is in turn connected to the control fluid connection 8 via a control fluid line.
  • the control arrangement 13 can be connected to a control fluid inlet 18 of the diaphragm pump 14 via a control fluid line 10 via the control fluid inlet 8.
  • the control fluid inlet 1 is connected to the collection point 6 via a first proportional valve 3 and the control fluid outlet 2 is connected to the collection point 6 via a second proportional valve 4.
  • signals are connected to a control unit 12.
  • a thermal mass flow sensor 5 and an absolute pressure sensor 7 are arranged between the collection point 6 and the control fluid connection 8.
  • the mass flow sensor 5 and the pressure sensor 7 are also connected to the control unit 12 for signaling purposes.
  • a temperature sensor 11 is provided which measures the ambient temperature and is also connected to the control unit 12 for signals via a temperature sensor interface 13B.
  • the control arrangement 13 also has a communication interface 13A which is designed for an exchange, in particular of data, with an external communication unit.
  • the communication interface 13A is preferably designed as a digital interface.
  • control fluid is conveyed from the first control fluid reservoir 1A via the first proportional valve 3 to the collecting point 6 and from there via the control fluid connection 8 to the diaphragm pump 14 in order to fill the control fluid-side chamber 16 there and in this way in the media-side chamber 17 of the diaphragm pump 14 to displace the medium located through the media outlet 19 of the membrane pump 14 and, for example, to dispense it into a sample container 41.
  • Control fluid is withdrawn from the control fluid connection 8 via the control fluid inlet 18 from the control fluid side chamber 16 of the diaphragm pump 14 via the collection point 6 and the second proportional valve 4 is drawn from the control fluid outlet 2 into the second control fluid reservoir 2A.
  • control fluid thus flows between the collecting point 6 and the control fluid connection 8 - depending on the conveying direction - in different flow directions.
  • the mass flow sensor 5 is therefore preferably designed to detect the mass flow of the control fluid independently of its direction of flow or to detect a detection of the mass flow both in a flow direction from the collection point 6 to the control fluid connection 8 and a flow direction from the control fluid connection 8 to the collection point 6.
  • the pressure sensor 7 is also preferably designed to detect the pressure of the control fluid independently of its direction of flow.
  • the mass flow sensor 5 is preferably designed as a thermal mass flow sensor for gaseous media, the measurement signal of which correlates with the molar mass flow of the gas.
  • the sensor-free diaphragm pump 14 can be activated by means of the control arrangement 13 in order to move the medium to be conveyed in the media-side chamber 17 of the diaphragm pump from the media inlet 20 to the media outlet 19 by the diaphragm 15 being volumetrically controlled by the control arrangement 13 by means of the control fluid line 10. and outflowing control fluid is moved.
  • control unit 12 of the control arrangement 13 is preferably designed to carry out the method 1000 shown in FIG. 2 for controlling the sensor-free diaphragm pump 14.
  • the first proportional valve 3 is first activated in step 1001 to release the flow and control fluid is conveyed in the direction of the diaphragm pump 14.
  • the first proportional valve 3 is closed as soon as a volume to be conveyed has been reached.
  • the second proportional valve 4 is activated to release the flow and control fluid is pumped from the diaphragm pump 14 to the second control fluid reservoir 2A, until finally in step 1004 the second proportional valve 4 is closed as soon as the volume to be pumped is reached.
  • the actually conveyed volume of the control fluid is preferably determined. This is preferably done as set out below.
  • the mass flow which correlates with the molar mass flow, is recorded with the mass flow sensor 5. In the case of a liquid control fluid, correlate this directly with the volume flow.
  • the output signal of the thermal mass flow sensor 5 preferably correlates with the molar mass flow of the control fluid.
  • the volume flow can be derived or calculated from this. The respective conditions, in particular the respective properties of the control fluid, and boundary parameters must be taken into account.
  • the volume flow of the control fluid can be calculated on the basis of the law of ideal gases or on the basis of a functional relationship adapted to the gaseous control fluid used, using the molar mass flow determined with the mass flow sensor 5, the absolute pressure p measured with the pressure sensor 7 and the the temperature sensor 1 1 detected temperature and the general gas constant are taken into account.
  • an approximately isothermal process can be used as a basis, or an adiabatic process. From the molar mass flow n determined with the mass flow sensor 5, first the molar number of particles N can be determined by integration over time.
  • the current volume V in the control fluid-side chamber 16 of the diaphragm pump 14 and thus also the corresponding displaced volume of the medium to be conveyed can preferably be calculated from this using the general gas law, taking into account the parameters measured by the pressure sensor 7 and the temperature sensor 11 .
  • the volume V determined in this way is therefore in direct correlation to the volumetric movement of the medium to be conveyed.
  • the method for controlling the sensor-free diaphragm pump 14 preferably also includes the detection of a pressure of the control fluid and the activation of the first and / or the second proportional valve 3, 4 in such a way that a predetermined target pressure of the control fluid is not exceeded and / or a predetermined target -The pressure range is complied with and / or a predetermined pressure gradient is complied with.
  • a volume difference between a first position of the membrane 15 with a correlating volume of the control fluid-side chamber 16 and a second position of the membrane with a second correlating volume of the control fluid-side chamber 16 can also be referred to as the difference volume dV. If the first and second positions of the membrane are their end positions, the total difference in volume results.
  • the Initialization of the integrator for calculating the total number of particles to be taken into account with a defined initial value is described below.
  • a volumetric control with a defined volume flow can take place, for example, as follows.
  • a volume dV of the medium to be conveyed to be conveyed should be conveyed with a predetermined volume flow.
  • control fluid is flown in a regulated manner into the control fluid side 16 of the diaphragm pump 14 from the control fluid reservoir 1A, the first proportional valve 3 being controlled by the control unit 12 as described above to determine the volume flow and the volume to be conveyed in such a way that the volume flow F corresponds to the predetermined values is adjusted and the process is completed after reaching the volume to be conveyed dV.
  • the medium to be conveyed is then sucked into the medium-side chamber 17 of the diaphragm pump 14 by a volume dV to be conveyed with a predetermined volume flow F, in that control fluid is flowed out of the control-fluid-side chamber 16 of the diaphragm pump 14 to the control fluid reservoir 2A, the second proportional valve 4 through the control unit 12 is controlled as described above to determine the volume flow and volume in such a way that the volume flow is regulated according to the predetermined values and the process is completed after the volume dV to be conveyed has been reached. Furthermore, it is preferred to additionally monitor the pressure acting on the control fluid and, in a correlating manner, also on the medium to be conveyed.
  • the pressure or pressure gradient of the control fluid is limited to predetermined limit values by measuring the pressure by the pressure sensor 7 and suitable interventions by the control unit 12 on the proportional valves 3, 4.
  • volumetric control with predetermined pressure and / or pressure gradients can be achieved.
  • a control method can preferably proceed as follows. First, the medium to be conveyed is displaced by a volume dV to be conveyed from the medium-side chamber 17 of the diaphragm pump 14 with a predetermined pressure profile, in particular a predetermined pressure curve over time, by a controlled inflow of the Control fluid into the control fluid-side chamber 16 of the diaphragm pump 14 from the control fluid reservoir 1A.
  • the first proportional valve 3 is controlled by the control unit 12, taking into account the detected absolute pressure p of the control fluid and the previously described method for determining the conveyed volume, in such a way that the control fluid pressure p is adjusted according to the predetermined values, and the process after reaching the promotional volume dV is completed.
  • the medium to be conveyed is then sucked into the medium-side chamber 17 of the diaphragm pump 14 by a controlled flow of the control fluid from the control-fluid-side chamber 16 of the diaphragm pump 14 to the control fluid reservoir by a volume dV to be conveyed with a predetermined pressure profile, in particular a predetermined pressure profile over time 2A.
  • the proportional valve 4 is controlled by the control unit 12 using the detected absolute pressure p and the previously described method for detecting the conveyed volume in such a way that the control fluid pressure p is regulated according to the specifications and the process is completed after the volume dV has been reached .
  • control arrangement 13 can also be used to determine the volumetric end positions as well as the intermediate reference points of the membrane 15 of the membrane pump 14. For this purpose, a predetermined low initialization volume flow is first impressed on the control fluid via the proportional valve 3 and thereby by means of the
  • Pressure sensor 7 continuously monitors the course of the pressure over time until its gradient becomes significantly steeper. This makes it possible to determine that the diaphragm 15 of the diaphragm pump 14 has reached one of its mechanical stops, i.e. one of its maximum positions or end positions. After this process has been carried out for the first end position by the control fluid flowing into the control fluid-side chamber 16 via the proportional valve 3 from the control fluid reservoir 1A, the process can be started in the opposite direction by drawing the control fluid from the control fluid-side chamber 16 of the diaphragm pump 14 also flows out via the proportional valve 4 to the control fluid reservoir 2A with a predetermined initialization volume flow.
  • the time profile of the pressure is continuously monitored by means of the pressure sensor 7 and the second end position is detected when the gradient rises significantly.
  • the total differential volume dVtotal which results between the two end positions of the membrane 15 of the membrane pump 14, is also determined using the method described above. Based on these Information can also be defined and controlled at any intermediate reference points of the membrane 15, for example the neutral position with half the total differential volume dVtotal.
  • the dead volume is preferably also determined as the initial value, which is formed by the sum of all volumes of the interconnected control fluid system elements from the two proportional valves 3, 4 to the diaphragm pump 14 in the end position of the diaphragm, which is a minimum volume of the control fluid-side chamber 16 corresponds.
  • This dead volume is due to the construction of the control arrangement and the control fluid line connecting the control arrangement to the diaphragm pump and is therefore easy to determine.
  • the corresponding end position of the membrane is activated and based on the determined dead volume and the conditions currently measured by the pressure sensor 7 and the temperature sensor 11 using the law of ideal gases or a functional relationship adapted to the control fluid used, the starting value of the particle number N of the integrator is calculated.
  • the control arrangement described here and the method for controlling a sensor-free diaphragm pump have a number of advantages over existing solutions.
  • Existing solutions that rely in particular on a distance sensor of a diaphragm pump to detect the position of the diaphragm are in principle associated with greater tolerances, since here the displaced volume is only determined indirectly and the spatial deformation of the elastic diaphragm cannot be reproducibly described.
  • the distance sensor for detecting the position of the diaphragm makes the diaphragm pump more expensive.
  • the handling effort by the user is increased and a system structure with only a low degree of integration is achieved.
  • the control arrangement described here and the method for controlling a sensor-free diaphragm pump enable position control of the diaphragm based on a volumetric determination and avoid the arrangement of sensor components in the immediate vicinity of the diaphragm pump. This enables a real or volumetric determination of the moving fluid volume instead of just a distance measurement of the membrane with non-reproducible deformation behavior.
  • a high degree of integration is achieved, since all the sensors required for the control can be accommodated in the control system (where, if necessary, a temperature sensor can also be connected via a temperature sensor interface).
  • the diaphragm pump to be controlled is sensor-free.

Landscapes

  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Hardware Design (AREA)
  • Reciprocating Pumps (AREA)
  • Control Of Positive-Displacement Pumps (AREA)

Abstract

L'invention concerne un dispositif de commande (13) servant à commander une pompe à membrane exempte de capteurs (14). L'invention concerne en outre un procédé de commande d'une pompe à membrane exempte de capteurs. Le dispositif de commande (13) comprend une entrée de fluide de commande (1) servant au raccord à un premier réservoir de fluide de commande (1A) présentant un niveau de pression augmenté par rapport à une pression de référence, une sortie de fluide de commande (2) servant au raccord à un second réservoir de fluide de commande (2A) comportant un niveau de pression réduit par rapport à une pression de référence, un raccord de fluide de commande (8) servant au raccord d'une conduite de fluide de commande destinée à la pompe à membrane exempte de capteurs (14), l'entrée de fluide de commande (1) étant reliée à un point de collecte (6) par l'intermédiaire d'une première soupape proportionnelle (3) et la sortie de fluide de commande (2) étant reliée au point de collecte (6) par l'intermédiaire d'une seconde soupape proportionnelle (4), un capteur de débit de masse (5) étant agencé entre le point de collecte (6) et le raccord de fluide de commande (8), et le dispositif de commande (13) comprenant en outre une unité de commande (12) qui est reliée par transmission de signaux à la première soupape proportionnelle (3), à la seconde soupape proportionnelle (4) et au capteur de débit de masse (5).
EP20731145.7A 2019-07-12 2020-06-12 Dispositif de commande et procédé de commande d'une pompe à membrane exempte de capteurs Pending EP3997339A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
LU101302 2019-07-12
PCT/EP2020/066354 WO2021008788A1 (fr) 2019-07-12 2020-06-12 Dispositif de commande et procédé de commande d'une pompe à membrane exempte de capteurs

Publications (1)

Publication Number Publication Date
EP3997339A1 true EP3997339A1 (fr) 2022-05-18

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Application Number Title Priority Date Filing Date
EP20731145.7A Pending EP3997339A1 (fr) 2019-07-12 2020-06-12 Dispositif de commande et procédé de commande d'une pompe à membrane exempte de capteurs

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Country Link
US (1) US20240209847A1 (fr)
EP (1) EP3997339A1 (fr)
JP (1) JP2022539428A (fr)
CN (1) CN114127419B (fr)
WO (1) WO2021008788A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3199616B1 (fr) 2016-01-29 2024-08-21 Eppendorf SE Dispositif de connexion a une voie
CN115489213B (zh) * 2022-11-21 2023-03-24 季华实验室 一种oled喷印墨路流量控制方法及系统

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6604908B1 (en) * 1999-07-20 2003-08-12 Deka Products Limited Partnership Methods and systems for pulsed delivery of fluids from a pump
WO2010069319A2 (fr) 2008-12-19 2010-06-24 Stobbe Tech A/S Procédé et dispositif pour la culture industrielle sur biocouches
WO2014076519A1 (fr) * 2012-10-29 2014-05-22 Debiotech S.A. Dispositif de traitement extra corporel du sang
FR3021074B1 (fr) * 2014-05-14 2016-05-27 Saint Gobain Performance Plastics France Pompe a membrane
DE102016015207A1 (de) * 2016-12-21 2018-06-21 Fresenius Medical Care Deutschland Gmbh Betätigungseinrichtung und Verfahren zum Betreiben einer Betätigungseinrichtung sowie Membranpumpe mit einer Betätigungseinrichtung und einer Membranpumpeneinrichtung und eine Blutbehandlungsvorrichtung mit einer Membranpumpe
US11306709B2 (en) * 2016-12-21 2022-04-19 Fresenius Medical Care Deutschland Gmbh Diaphragm pump device and diaphragm pump having a diaphragm pump device and an actuation device

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JP2022539428A (ja) 2022-09-08
CN114127419B (zh) 2024-04-26
WO2021008788A1 (fr) 2021-01-21
US20240209847A1 (en) 2024-06-27
CN114127419A (zh) 2022-03-01

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