EP3980558A4 - METHODS AND REAGENTS FOR THE AMPLIFICATION AND/OR DETECTION OF NUCLEIC ACIDS - Google Patents

METHODS AND REAGENTS FOR THE AMPLIFICATION AND/OR DETECTION OF NUCLEIC ACIDS Download PDF

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Publication number
EP3980558A4
EP3980558A4 EP20819192.4A EP20819192A EP3980558A4 EP 3980558 A4 EP3980558 A4 EP 3980558A4 EP 20819192 A EP20819192 A EP 20819192A EP 3980558 A4 EP3980558 A4 EP 3980558A4
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EP
European Patent Office
Prior art keywords
reagents
amplification
detection
methods
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20819192.4A
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German (de)
English (en)
French (fr)
Other versions
EP3980558A1 (en
Inventor
Peter J. UNRAU
Amir ABDOLAHZADEH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Simon Fraser University
Original Assignee
Simon Fraser University
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Publication date
Application filed by Simon Fraser University filed Critical Simon Fraser University
Publication of EP3980558A1 publication Critical patent/EP3980558A1/en
Publication of EP3980558A4 publication Critical patent/EP3980558A4/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP20819192.4A 2019-06-07 2020-06-07 METHODS AND REAGENTS FOR THE AMPLIFICATION AND/OR DETECTION OF NUCLEIC ACIDS Pending EP3980558A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962858874P 2019-06-07 2019-06-07
PCT/IB2020/055348 WO2020245808A1 (en) 2019-06-07 2020-06-07 Methods and reagents for nucleic acid amplification and/or detection

Publications (2)

Publication Number Publication Date
EP3980558A1 EP3980558A1 (en) 2022-04-13
EP3980558A4 true EP3980558A4 (en) 2023-07-19

Family

ID=73652122

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20819192.4A Pending EP3980558A4 (en) 2019-06-07 2020-06-07 METHODS AND REAGENTS FOR THE AMPLIFICATION AND/OR DETECTION OF NUCLEIC ACIDS

Country Status (6)

Country Link
US (1) US20220307080A1 (https=)
EP (1) EP3980558A4 (https=)
JP (2) JP2022535584A (https=)
KR (1) KR20220018036A (https=)
CA (1) CA3142670A1 (https=)
WO (1) WO2020245808A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021202504A1 (en) * 2020-04-29 2021-11-18 Teleflex Medical Incorporated System for identifying severe acute respiratory syndrome corona virus 2 (sars-cov-2) ribonucleic acid (rna)
WO2022040355A2 (en) * 2020-08-19 2022-02-24 City Of Hope Lamp detection of sars-cov-2 in saliva for the rapid diagnosis of covid-19
WO2022178313A1 (en) * 2021-02-18 2022-08-25 The Johns Hopkins University Detecting diseases in subjects using extracelluar vesicles
CN113061650B (zh) * 2021-02-19 2024-07-16 中国科学院深圳先进技术研究院 一种病原体核酸的即时检测系统及方法
CN113234798B (zh) * 2021-04-26 2023-04-11 重庆医科大学 基于体外转录和G4-ThT的荧光传感器及其制备与在HBV DNA检测上的应用
WO2022236020A1 (en) * 2021-05-07 2022-11-10 Prime Discoveries, Inc. An isothermal amplification system and method of use of the same for detecting pathogenic infection in a subject
CN113308575B (zh) * 2021-06-10 2022-04-01 安徽医科大学 一种检测筛查新冠病毒n439k突变的方法及试剂盒
GB202110391D0 (en) 2021-07-19 2021-09-01 Tissue Click Ltd A detection kit and methods of detection of infectious agents
WO2023152481A1 (en) * 2022-02-09 2023-08-17 Imperial College Innovations Limited Nucleic acid detection
WO2025206261A1 (ja) * 2024-03-29 2025-10-02 国立大学法人 東京大学 デジタルアッセイ方法及びデジタルアッセイキット
WO2025240876A1 (en) * 2024-05-17 2025-11-20 Board Of Regents, The University Of Texas System Optimized fluorogenic aptamers and methods of identification and use thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7723078B2 (en) * 2001-03-07 2010-05-25 Biomerieux, B.V. Method for the amplification and detection of HBV DNA using a transcription based amplification
KR20140029825A (ko) * 2012-08-30 2014-03-11 한국과학기술원 표적물질의 결합에 의한 압타머 구조 변화 특성을 이용한 등온 핵산 증폭방법 및 이를 이용한 초고감도 생체물질 검출방법
WO2018075502A1 (en) * 2016-10-17 2018-04-26 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection
WO2018112350A1 (en) * 2016-12-15 2018-06-21 Arizona Board Of Regents On Behalf Of Arizona State University Molecular fuses for real-time, label-free, multiplexed imaging of rnas in living cells
WO2018198013A1 (en) * 2017-04-24 2018-11-01 Centre National De La Recherche Scientifique Fluorogen-binding rna aptamers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7723078B2 (en) * 2001-03-07 2010-05-25 Biomerieux, B.V. Method for the amplification and detection of HBV DNA using a transcription based amplification
KR20140029825A (ko) * 2012-08-30 2014-03-11 한국과학기술원 표적물질의 결합에 의한 압타머 구조 변화 특성을 이용한 등온 핵산 증폭방법 및 이를 이용한 초고감도 생체물질 검출방법
WO2018075502A1 (en) * 2016-10-17 2018-04-26 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection
WO2018112350A1 (en) * 2016-12-15 2018-06-21 Arizona Board Of Regents On Behalf Of Arizona State University Molecular fuses for real-time, label-free, multiplexed imaging of rnas in living cells
WO2018198013A1 (en) * 2017-04-24 2018-11-01 Centre National De La Recherche Scientifique Fluorogen-binding rna aptamers

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AMIR ABDOLAHZADEH ET AL: "RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA", RNA, vol. 25, 24 September 2019 (2019-09-24), pages 1806 - 1813, XP055766724, DOI: 10.1261/rna *
S. FUKUDA ET AL: "Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 12, 15 June 2008 (2008-06-15), US, pages 3912 - 3914, XP055418123, ISSN: 0099-2240, DOI: 10.1128/AEM.00127-08 *
See also references of WO2020245808A1 *
ZHANG LIU LI HOU XUE XIA GENG ZHEN LOU YONG LIANG WAN KANG LIN HAO QIN ET AL: "Combination of Loop-Mediated Isothermal Amplification Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples", ?????????????, 1 January 2015 (2015-01-01), United States, pages 312 - 315, XP055320897, Retrieved from the Internet <URL:http://www.besjournal.com/Articles/Archive/2015/No4/201505/P020150512322006188037.pdf> DOI: 10.3967/bes2015.044 *

Also Published As

Publication number Publication date
JP2025162555A (ja) 2025-10-27
US20220307080A1 (en) 2022-09-29
WO2020245808A1 (en) 2020-12-10
CA3142670A1 (en) 2020-12-10
EP3980558A1 (en) 2022-04-13
KR20220018036A (ko) 2022-02-14
JP2022535584A (ja) 2022-08-09

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