EP3894541A1 - Mélange de micro-organismes fluide à durée de conservation prolongée pour éliminer les composants atp contenus dans les eaux usées et son procédé de production - Google Patents

Mélange de micro-organismes fluide à durée de conservation prolongée pour éliminer les composants atp contenus dans les eaux usées et son procédé de production

Info

Publication number
EP3894541A1
EP3894541A1 EP20744557.8A EP20744557A EP3894541A1 EP 3894541 A1 EP3894541 A1 EP 3894541A1 EP 20744557 A EP20744557 A EP 20744557A EP 3894541 A1 EP3894541 A1 EP 3894541A1
Authority
EP
European Patent Office
Prior art keywords
bacterial
bacterial culture
bacteria
fluid form
nitrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20744557.8A
Other languages
German (de)
English (en)
Other versions
EP3894541A4 (fr
Inventor
Guven OZDEMIR
Tayyibe ALPAY
Didem EROGLU
Burcin SAYGILI
Caner Vural
Ayhan Ezdesir
Hursel CAY
Bike PASHAYEVA OGUNLU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Petkim Petrokimya Holding Anonim Sirekti
Original Assignee
Petkim Petrokimya Holding Anonim Sirekti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Petkim Petrokimya Holding Anonim Sirekti filed Critical Petkim Petrokimya Holding Anonim Sirekti
Publication of EP3894541A1 publication Critical patent/EP3894541A1/fr
Publication of EP3894541A4 publication Critical patent/EP3894541A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/343—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of grease, fat, oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/32—Hydrocarbons, e.g. oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
    • C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
    • C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)

Definitions

  • the invention is related to the formation of bacterial culture products in fluid form which has high biodegradation efficiency, is environmentally friendly, in the presence of an appropriate substrate relative to the hydrocarbon types in order to be used in biological treatment applications of various petrochemical oil and refinery plants.
  • the invention is particularly related to production of adapted bacterial cultures in fluid form and with high efficiency and performance by means of reproduction thereof in the presence of appropriate substrate in reactor systems, which will be used in the treatment of pure terephthalic acid (PTA) waste waters with a hard-to-degrade xenobiotic characteristic.
  • PTA terephthalic acid
  • the microorganisms are able to degrade the hydrocarbons which are both naturally formed and are included in the structure of the environmental pollutants. It is found in the studies conducted so far that the microorganisms are able to degrade various pollutants which can be found in the structure of phenol, benzoate, eicosane, 2,4- dichlorofenoxyacetic acid (2,4-D), polyaromatic hydrocarbons (PAH), xenobiotic hydrocarbons and crude oil under appropriate conditions.
  • the patent document No“KR0000034035” is analyzed as a result of the preliminary search made about the state of the art.
  • the most important aim of the invention is to provide a fluid bacterial culture which is adaptable for PTA wastewater treatment, has high biodegradation efficiency and transform thereof into a stabilized bacterial culture with extended shelf life by means of reproduction. Another important aim of the invention is to adapt them easily to the system on which they have effect and thus to provide the quick efficiency of the bacterial cultures.
  • Another important aim of the invention is to reduce costs for said process by means of the high biodegradation efficiency and system adaptation (that the production has low cost)
  • FIGURE -1 is a graph which gives change of the living microorganism concentrations per month in relation of the shelf life studies.
  • the invention is particularly related to production of adapted bacterial cultures in fluid form and with high efficiency and performance by means of reproduction thereof in the presence of appropriate substrate in reactor systems, which will be used in the treatment of pure terephthalic acid PTA waste waters with a hard-to-degrade xenobiotic characteristic.
  • the stabilized bacterial culture in fluid form with extended shelf life which has high biodegradation efficiency mainly consists of; bacterial mixture isolated from the active sludge, distilled water and various nitrate salts.
  • Pseudomonadaceae In the content of the specific bacterial mixture isolated from the active sludge, at least one of the following families exist; Pseudomonadaceae, Comamonadaceae.
  • Pseudomonadaceae Bacteria is a family within the domain and bacteria species such as Azomonas, Azomonotrichon, Azorhizophilus, Azotobacter, Cellvibrio, Mesophilobacter, Pseudomonas, Rhizobacter, Rugamonas, and Serpens etc. are included within this family.
  • Comamonadaceae is a wide and various bacterial family.
  • Bacteria species such as Acidovorax, Albidiferax, Alicycliphilus, Brachymonas, Caenimonas, Comamonas, Curvibacter, Delftia, Diaphorobacter, Extensimonas, Giesbergeria, Hydrogenophaga, Hylemonella etc. are included within this family.
  • Pseudomonadaceae, Comamonadaceae families are isolated from the current wastewater treatment system which is their natural habitats, thus their adaptation to the system is very easy and rapid.
  • nitrate salts Nitrogen ions in the nitrate salts content are converted to nitrogen gas by a number of microorganisms to produce energy. This event is called denitrification. Denitrification bacteria obtain the required energy for their growth under anoxic conditions in this manner and therefore their viability and amount, thus their shelf life is protected. It is known that numerous types of bacteria such as Pseudomonas stutzeri etc. make denitrification by using nitrate or nitrite as the main electron for anaerobic respiration.
  • the nitrate salts are the salts of nitric acid and among its types there is sodium nitrate, potassium nitrate, ammonium nitrate.
  • the distilled water is obtained by means of boiling the normal tap water at a predetermined temperature and separating the pathogenic substances therein and thus re-condensing thereof and forming water again.
  • Table 1 A table showing the components and amounts of the reproduced bacteria isolates
  • the components included in the above table are used, the final product stabilized bacterial culture in fluid form is obtained produced by means of the below described method.
  • Production of bacterial culture in fluid form consists of the following process steps,
  • the shelf life studies of the product which is obtained after said process steps were made at 20-35°C temperature, acid degree of pH 6-8.
  • the shelf life studies were started with at least 10 10 kob/ml microorganism initial microorganism and it is detected that at the end of 6 moths the number was 10 4 -10 8 kob/ml. Accordingly, at least month 6 shelf life is determined with optimum temperature of 20-35°C, and pH 6-8.
  • the graph showing the change in the number of the living microorganisms according to months in shelf life studies is shown in Figure 1 .
  • Different storage conditions are determined in a manner such that the degradation of bacterial cultures and viability efficiency is optimum by means of making pre-tests in obtaining fluid product with high bio-degradation for PTA wastewater subject to the invention.
  • the efficiency of these microorganisms produced in this context in the fractionation of chemicals which constitutes PTA wastewater such as terephthalic acid (TA), 4-carboxybenzaldehyde (4-CBA) and paratoluic acid (P-tol) etc. at the end of the shelf life is evaluated.
  • Optimum rates pertaining to the product content were determined by making preliminary tests in the study. Therefore, these percentage rates are important in terms of bacterial viability concentration of the final product and in case there are changes in the rates, when we consider the bacterial viability, this can lead to unexpected decreases in application of both final product and in environmental system. In this case, there will be no improvement in the performance of the treatment process which is the final target.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne la production d'une culture bactérienne sous forme fluide qui a une efficacité de biodégradation élevée et qui est respectueuse de l'environnement pour une utilisation dans le traitement des eaux usées de production d'acide téréphtalique purifié (ATP).
EP20744557.8A 2019-01-23 2020-01-20 Mélange de micro-organismes fluide à durée de conservation prolongée pour éliminer les composants atp contenus dans les eaux usées et son procédé de production Pending EP3894541A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2019/01053A TR201901053A2 (fr) 2019-01-23 2019-01-23
PCT/TR2020/050037 WO2020153928A1 (fr) 2019-01-23 2020-01-20 Mélange de micro-organismes fluide à durée de conservation prolongée pour éliminer les composants atp contenus dans les eaux usées et son procédé de production

Publications (2)

Publication Number Publication Date
EP3894541A1 true EP3894541A1 (fr) 2021-10-20
EP3894541A4 EP3894541A4 (fr) 2023-06-28

Family

ID=71736508

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20744557.8A Pending EP3894541A4 (fr) 2019-01-23 2020-01-20 Mélange de micro-organismes fluide à durée de conservation prolongée pour éliminer les composants atp contenus dans les eaux usées et son procédé de production

Country Status (3)

Country Link
EP (1) EP3894541A4 (fr)
TR (1) TR201901053A2 (fr)
WO (1) WO2020153928A1 (fr)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09174089A (ja) * 1995-12-26 1997-07-08 Toray Ind Inc テレフタル酸含有廃液の高温好気的処理方法
US6365397B1 (en) 1999-11-12 2002-04-02 Shell Oil Company Bacterial culture which degrades methyl-tert-butyl ether to carbon dioxide
GB0407329D0 (en) * 2004-03-31 2004-05-05 Danisco Process
ITMI20061841A1 (it) * 2006-09-27 2008-03-28 Mofin S R L Colture liquide microbiche ad elevata stabilita' ed attivita' fermentativa
CN100537746C (zh) 2006-10-13 2009-09-09 北京工商大学 具有好氧反硝化性能的戴尔福特菌及其处理废水的方法
CA2839383C (fr) 2011-01-12 2021-03-02 Inocucor Technologies, Inc. Composition renfermant un microorganisme destinee a la biomediation et a un usage agricole
NL2014777B1 (en) * 2015-05-07 2017-01-26 Ibema Biezenmortel B V Nitrifying micro-organisms for fertilisation.

Also Published As

Publication number Publication date
TR201901053A2 (fr) 2020-08-21
WO2020153928A1 (fr) 2020-07-30
EP3894541A4 (fr) 2023-06-28

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