EP3877388B1 - 5-[6-[[3-(4,5,6,7-tetrahydropyrazolo[4,3-c]pyridin-1-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile derivatives and similar compounds as tlr7-9 antagonists for treating systemic lupus erythematosus - Google Patents

5-[6-[[3-(4,5,6,7-tetrahydropyrazolo[4,3-c]pyridin-1-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile derivatives and similar compounds as tlr7-9 antagonists for treating systemic lupus erythematosus Download PDF

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Publication number
EP3877388B1
EP3877388B1 EP19805549.3A EP19805549A EP3877388B1 EP 3877388 B1 EP3877388 B1 EP 3877388B1 EP 19805549 A EP19805549 A EP 19805549A EP 3877388 B1 EP3877388 B1 EP 3877388B1
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Prior art keywords
methyl
tetrahydropyrazolo
morpholin
quinoline
carbonitrile
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German (de)
English (en)
French (fr)
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EP3877388A1 (en
EP3877388C0 (en
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Haixia Liu
Hong Shen
Wei Zhu
Fabian Dey
Xiaoqing Wang
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B43/00—Formation or introduction of functional groups containing nitrogen
    • C07B43/06—Formation or introduction of functional groups containing nitrogen of amide groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems

Definitions

  • the present invention relates to organic compounds useful for therapy and/or prophylaxis in a mammal, and in particular to antagonist of TLR7 and/or TLR8 and/or TLR9 useful for treating systemic lupus erythematosus or lupus nephritis.
  • Autoimmune connective tissue disease include prototypical autoimmune syndromes such as Systemic Lupus Erythematosus (SLE), primary Sjögren's syndrome (pSjS), mixed connective tissue disease (MCTD), Dermatomyositis/Polymyositis (DM/PM), Rheumatoid Arthritis (RA), and systemic sclerosis (SSc).
  • SLE represents the prototypical CTD with a prevalence of 20-150 per 100,000 and causes broad inflammation and tissue damage in distinct organs, from commonly observed symptoms in the skin and joints to renal, lung, or heart failure.
  • SLE has been treated with nonspecific anti-inflammatory or immunosuppressive drugs.
  • immunosuppressive drug e.g. corticosteroids
  • corticosteroids e.g. corticosteroids
  • Belimumab is the only FDA-approved drug for lupus in the last 50 years, despite its modest and delayed efficacy in only a fraction of SLE patients ( Navarra, S. V. et al Lancet 2011, 377, 721 .).
  • Other biologics such as anti-CD20 mAbs, mAbs against or soluble receptors of specific cytokines, have failed in most clinical studies.
  • novel therapies are required that provide sustained improvement in a greater proportion of patient groups and are safer for chronic use in many autoimmune as well as autoinflammation diseases.
  • TLR Toll Like Receptors
  • PRR pattern recognition receptors
  • endosomal TLRs 7, 8 and 9 recognize nucleic acids derived from viruses, bacteria; specifically, TLR7/8 and TLR9 recognize single-stranded RNA (ssRNA) and single-stranded CpG-DNA, respectively.
  • ssRNA single-stranded RNA
  • CpG-DNA single-stranded CpG-DNA
  • Anti-RNA and anti-DNA antibodies are well established diagnostic markers of SLE, and these antibodies can deliver both self-RNA and self-DNA to endosomes. While self-RNA complexes can be recognized by TLR7 and TLR8, self-DNA complexes can trigger TLR9 activation. Indeed, defective clearance of self-RNA and self-DNA from blood and/or tissues is evident in SLE (Systemic Lupus Erythematosus) patients. TLR7 and TLR9 have been reported to be upregulated in SLE tissues, and correlate with chronicity and activity of lupus nephritis, respectively.
  • TLR7 expression correlates with anti-RNP antibody production, while TLR9 expression with IL-6 and anti-dsDNA antibody levels. Consistently, in lupus mouse models, TLR7 is required for anti-RNA antibodies, and TLR9 is required for anti-nucleosome antibody. On the other hand, overexpression of TLR7 or human TLR8 in mice promotes autoimmunity and autoinflammation. Moreover, activation of TLR8 specifically contributes to inflammatory cytokine secretion of mDC/macrophages, neutrophil NETosis, induction of Th17 cells, and suppression of Treg cells.
  • TLR9 In addition to the described role of TLR9 in promoting autoantibody production of B cells, activation of TLR9 by self-DNA in pDC also leads to induction of type I IFNs and other inflammatory cytokines. Given these roles of TLR9 in both pDC and B cells, both as key contributors to the pathogenesis of autoimmune diseases, and the extensive presence of self-DNA complexes that could readily activate TLR9 in many patients with autoimmune diseases, it may have extra benefit to further block self-DNA mediated TLR9 pathways on top of inhibition of TLR7 and TLR8 pathways.
  • TLR7, 8, and 9 pathways represent new therapeutic targets for the treatment of autoimmune and auto-inflammatory diseases, for which no effective steroid-free and non-cytotoxic oral drugs exist, and inhibition of all these pathways from the very upstream may deliver satisfying therapeutic effects.
  • the present invention relates to novel compounds of formula (I) or (Ia), wherein
  • Another object of the present invention is related to novel compounds of formula (I) or (Ia), their manufacture, medicaments based on a compound in accordance with the invention and their production as well as the use of compounds of formula (I) or (Ia) as TLR7 and/or TLR8 and/or TLR9 antagonist, and for the treatment or prophylaxis of systemic lupus erythematosus or lupus nephritis.
  • the compounds of formula (I) or (Ia) show superior TLR7 and/or TLR8 and/or TLR9 antagonism activity.
  • the compounds of formula (I) or (Ia) also show good cytotoxicity, solubility, hPBMC, human microsome stability and SDPK profiles, as well as low CYP inhibition.
  • C 1-6 alkyl denotes a saturated, linear or branched chain alkyl group containing 1 to 6, particularly 1 to 4 carbon atoms, for example methyl, ethyl, n -propyl, isopropyl, n -butyl, isobutyl, tert -butyl and the like.
  • Particular “C 1-6 alkyl” groups are methyl, ethyl and n -propyl.
  • halogen and "halo” are used interchangeably herein and denote fluoro, chloro, bromo, or iodo.
  • haloC 1-6 alkyl denotes a C 1-6 alkyl group wherein at least one of the hydrogen atoms of the C 1-6 alkyl group has been replaced by same or different halogen atoms, particularly fluoro atoms.
  • haloC 1-6 alkyl include monofluoro-, difluoro- or trifluoro-methyl, - ethyl or -propyl, for example 3,3,3-trifluoropropyl, 2-fluoroethyl, trifluoroethyl, fluoromethyl, difluoromethyl, difluoroethyl or trifluoromethyl.
  • LG denotes a leaving group, which is a molecular fragment that departs with a pair of electrons in heterolytic bond cleavage. Leaving groups can be anions or neutral molecules, but in either case it is crucial that the leaving group be able to stabilize the additional electron density that results from bond heterolysis. Common anionic leaving groups are halides, and sulfonate esters such as OTf, OTs and OMs.
  • PG denotes a protecting group, which is introduced into a molecule by chemical modification of a functional group to obtain chemo selectivity in a subsequent chemical reaction.
  • Typical protecting groups are Boc, Cbz and Bn.
  • diastereomer denotes a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, and reactivities.
  • pharmaceutically acceptable salts denotes salts which are not biologically or otherwise undesirable.
  • Pharmaceutically acceptable salts include both acid and base addition salts.
  • pharmaceutically acceptable acid addition salt denotes those pharmaceutically acceptable salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, carbonic acid, phosphoric acid, and organic acids selected from aliphatic, cycloaliphatic, aromatic, araliphatic, heterocyclic, carboxylic, and sulfonic classes of organic acids such as formic acid, acetic acid, propionic acid, glycolic acid, gluconic acid, lactic acid, pyruvic acid, oxalic acid, malic acid, maleic acid, maloneic acid, succinic acid, fumaric acid, tartaric acid, citric acid, aspartic acid, ascorbic acid, glutamic acid, anthranilic acid, benzoic acid, cinnamic acid, mandelic acid, embonic acid, phenylacetic acid, methanesulfonic acid, ethanesulfonic acid, p -toluen
  • pharmaceutically acceptable base addition salt denotes those pharmaceutically acceptable salts formed with an organic or inorganic base.
  • acceptable inorganic bases include sodium, potassium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, and aluminum salts.
  • Salts derived from pharmaceutically acceptable organic nontoxic bases includes salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-diethylaminoethanol, trimethamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperizine, piperidine, N -ethylpiperidine, and polyamine resins.
  • substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, trie
  • a pharmaceutically active metabolite denotes a pharmacologically active product produced through metabolism in the body of a specified compound or salt thereof. After entry into the body, most drugs are substrates for chemical reactions that may change their physical properties and biologic effects. These metabolic conversions, which usually affect the polarity of the compounds of the invention, alter the way in which drugs are distributed in and excreted from the body. However, in some cases, metabolism of a drug is required for therapeutic effect.
  • therapeutically effective amount denotes an amount of a compound or molecule of the present invention that, when administered to a subject, (i) treats or prevents the particular disease, condition or disorder, (ii) attenuates, ameliorates or eliminates one or more symptoms of the particular disease, condition, or disorder, or (iii) prevents or delays the onset of one or more symptoms of the particular disease, condition or disorder described herein.
  • the therapeutically effective amount will vary depending on the compound, the disease state being treated, the severity of the disease treated, the age and relative health of the subject, the route and form of administration, the judgement of the attending medical or veterinary practitioner, and other factors.
  • composition denotes a mixture or solution comprising a therapeutically effective amount of an active pharmaceutical ingredient together with pharmaceutically acceptable excipients to be administered to a mammal, e.g., a human in need thereof.
  • the present invention relates to a compound of formula (I), wherein
  • Another embodiment of present invention is (ii) a compound of formula (Ia), wherein
  • a further embodiment of present invention is (iii) a compound of formula (I) or (Ia) according to (i) or (ii), wherein
  • a further embodiment of present invention is (iv) a compound of formula (I) or (Ia), or a pharmaceutically acceptable salt thereof, according to any one of (i) to (iii), wherein R 1 is wherein R 4 is cyano.
  • a further embodiment of present invention is (v) a compound of formula (I) or (Ia), or a pharmaceutically acceptable salt thereof according to any one of (i) to (iv), wherein L is azetidinyl.
  • a further embodiment of present invention is (vi) a compound of formula (I) or (Ia), or a pharmaceutically acceptable salt thereof, according to any one of (i) to (v), wherein R 2 is 4,5,6,7-tetrahydropyrazolo[3,4-c]pyridinyl substituted by C 1-6 alkyl; or 4,5,6,7-tetrahydropyrazolo[4,3-c]pyridinyl substituted by C 1-6 alkyl.
  • a further embodiment of present invention is (vii) a compound of formula (I) or (Ia), or a pharmaceutically acceptable salt thereof, according to any one of (i) to (vi), wherein R 2 is 4,5,6,7-tetrahydropyrazolo[3,4-c]pyridinyl substituted by methyl; or 4,5,6,7-tetrahydropyrazolo[4,3-c]pyridinyl substituted by methyl.
  • Another embodiment of present invention is (viii) a compound of formula (I) or (Ia) according to (i) or (ii), wherein
  • a further embodiment of present invention is (ix) a compound of formula (I) or (Ia) according to (viii), wherein
  • TLR7 and TLR9 potency data summarized in table 1 (TLR8 data is not available).
  • Compounds in Table 1 are all characterized with an aromatic ring at the terminal position (phenyl or pyridinyl).
  • TLR7 potency phenyl or pyridinyl
  • the compounds of this invention significantly improved TLR9 potency (>8 folds compared to ER-888286) while keeping excellent TLR7 and TLR8 potency.
  • hERG profile and safety ratio were greatly improved as compared with reference compounds from US20150105370 and reference compounds R1 and R2 synthesized herein (see table 3).
  • the compounds of formula (I) or (Ia) also showed good hPBMC, cytotoxicity, solubility, human microsome stability and SDPK profiles, as well as low CYP inhibition. Table 1.
  • TLR7 and TLR9 potency of compounds disclosed in US20150105370 Compound Structure HEK/hTLR7 IC50 ( ⁇ M) HEK/hTLR9 IC50 ( ⁇ M) ER-887258 0.0852 >2.0 ER-888285 0.120 >2.0 ER-888286 1.370 >2.0 ER-894544 0.043 >6.2 ER-894160 0.1990 >10.0 ER-894155 0.2820 >10.0
  • the compounds of the present invention can be prepared by any conventional means. Suitable processes for synthesizing these compounds as well as their starting materials are provided in the schemes below and in the examples. All substituents, in particular, R 1 to R 4 are as defined above unless otherwise indicated. Furthermore, and unless explicitly otherwise stated, all reactions, reaction conditions, abbreviations and symbols have the meanings well known to a person of ordinary skill in organic chemistry.
  • LG is a leaving group, such as halogen, OTf, OTs and OMs
  • PG is a protecting group, such as Boc and Cbz.
  • the coupling of compound of formula (II) with R 1 -X can be achieved by direct coupling at elevated temperature in the presence of a base, such as DIPEA or K 2 CO 3 , or under Buchwald-Hartwig amination conditions (ref: Acc. Chem. Res. 1998, 31, 805-818 ; Chem. Rev. 2016, 116, 12564-12649 ; Topics in Current Chemistry, 2002, 219, 131-209 ; and references cited therein) with a catalyst, such as RuPhos Pd G2, and a base, such as Cs 2 CO 3 or K 2 CO 3 , to provide compound of formula (IV).
  • a base such as DIPEA or K 2 CO 3
  • Buchwald-Hartwig amination conditions ref: Acc. Chem. Res. 1998, 31, 805-818 ; Chem. Rev. 2016, 116, 12564-12649 ; Topics in Current Chemistry, 2002, 219, 131-209 ; and references cited therein
  • a catalyst such as Ru
  • the hydroxy group of compound of formula (IV) is converted to a leaving group, such as OTf, OTs, or OMs, under basic condition, such as DIPEA, TEA, K 2 CO 3 or 2,6-dimethylpyridine, with Tf 2 O, TsCl or MsCl.
  • the coupling of compound of formula (VI) with (VII) can be achieved by direct coupling at high temperature in the presence of a base, such as NaH or Cs 2 CO 3 to provide compound of formula (VIII).
  • the protecting group of compound of formula (VIII) can be removed at high temperature or under acidic condition, such as TFA, or under hydrogenation condition with a catalyst, such as Pd/C or Pd(OH) 2 /C.
  • Compound of formula (IX) is further coupled with compound of formula (V) in the presence of a base, such as K 2 CO 3 , DIPEA or Cs 2 CO 3 , to afford compound of formula (I).
  • a base such as K 2 CO 3 , DIPEA or Cs 2 CO 3
  • the coupling of compound of formula (IX) and (V) may give a product containing a protecting group, e.g. Boc or Cbz, originated from (IX), which will be removed before affording the final compound of formula (I).
  • the compound of formula (I) or (Ia) with a terminal secondary amine is further introduced with an alkyl group, such as methyl, ethyl or isopropyl, through reductive amination reaction under reductive condition, such as NaBH 3 CN or NaBH(OAc) 3 with formaldehyde, acetaldehyde or acetone to afford the final compound of formula (I) or (Ia).
  • an alkyl group such as methyl, ethyl or isopropyl
  • reductive amination reaction under reductive condition such as NaBH 3 CN or NaBH(OAc) 3 with formaldehyde, acetaldehyde or acetone
  • This invention also relates to a process for the preparation of a compound of formula (I) or (Ia) comprising the following step:
  • a compound of formula (I) or (Ia) when manufactured according to the above process is also an object of the invention.
  • the present invention provides compounds that can be used as TLR7 and/or TLR8 and/or TLR9 antagonist, which inhibits pathway activation through TLR7 and/or TLR8 and/or TLR9 as well as respective downstream biological events including, but not limited to, innate and adaptive immune responses mediated through the production of all types of cytokines and all forms of auto-antibodies. Accordingly, the compounds of the invention are useful for blocking TLR7 and/or TLR8 and/or TLR9 in all types of cells that express such receptor(s) including, but not limited to, plasmacytoid dendritic cell, B cell, T cell, macrophage, monocyte, neutrophil, keratinocyte, epithelial cell. As such, the compounds can be used as a therapeutic or prophylactic agent for systemic lupus erythematosus and lupus nephritis.
  • the present invention provides methods for treatment or prophylaxis of systemic lupus erythematosus and lupus nephritis in a patient in need thereof.
  • Another embodiment includes a method of treating or preventing systemic lupus erythematosus and lupus nephritis in a mammal in need of such treatment, wherein the method comprises administering to said mammal a therapeutically effective amount of a compound of formula (I), a stereoisomer, tautomer, or pharmaceutically acceptable salt thereof.
  • Waters AutoP purification System (Sample Manager 2767, Pump 2525, Detector: Micromass ZQ and UV 2487, solvent system: acetonitrile and 0.1% ammonium hydroxide in water; acetonitrile and 0.1% FA in water or acetonitrile and 0.1% TFA in water).
  • Or Gilson-281 purification System (Pump 322, Detector: UV 156, solvent system: acetonitrile and 0.05% ammonium hydroxide in water; acetonitrile and 0.225% FA in water; acetonitrile and 0.05% HCl in water; acetonitrile and 0.075% TFA in water; or acetonitrile and water).
  • LC/MS spectra of compounds were obtained using a LC/MS (Waters TM Alliance 2795-Micromass ZQ, Shimadzu Alliance 2020-Micromass ZQ or Agilent Alliance 6110-Micromass ZQ), LC/MS conditions were as follows (running time 3 or 1.5 mins):
  • the microwave assisted reactions were carried out in a Biotage Initiator Sixty microwave synthesizer. All reactions involving air-sensitive reagents were performed under an argon or nitrogen atmosphere. Reagents were used as received from commercial suppliers without further purification unless otherwise noted.
  • Step 1 preparation of [(2 R ,6 R )-6-methylmorpholin-2-yl]methanol;2,2,2-trifluoroacetic acid (compound A1)
  • Step 2 preparation of 5-[(2 R ,6 R )-2-(hydroxymethyl)-6-methyl-morpholin-4-yl]quinoline-8-carbonitrile (compound A3)
  • Step 3 preparation of [(2 R ,6 R )-4-(8-cyano-5-quinolyl)-6-methyl-morpholin-2-yl]methyl trifluoromethanesulfonate (Intermediate A)
  • Step 1 preparation of tert -butyl 4-(dimethylaminomethylene)-2-methyl-5-oxo-piperidine-1-carboxylate (compound C2)
  • Step 2 preparation of tert -butyl 5-methyl-2,4,5,7-tetrahydropyrazolo[3,4-c]pyridine-6-carboxylate (Intermediate C)
  • Intermediate F and G were prepared in analogy to the preparation of Intermediate C by using tert -butyl (2 R )-2-methyl-4-oxo-piperidine-1-carboxylate (CAS: 790667-43-5 , Vendor: Pharmablock) instead of tert -butyl 2-methyl-5-oxo-piperidine-1-carboxylate (compound C1 ).
  • Intermediate F (slower eluting) and Intermediate G (faster eluting) were separated by chiral SFC (Gradient: 20% MeOH in CO 2 , Column: ChiralPak AD, 300 ⁇ 50mm I.D., 10 ⁇ m).
  • Intermediate F (700 mg) was obtained as a yellow oil.
  • Intermediate G (580 mg) was obtained as a yellow oil.
  • Step 1 preparation of tert -butyl 4-acetyl-3-oxo-piperidine-1-carboxylate (compound J2)
  • Step 2 preparation of tert -butyl 3-methyl-2,4,5,7-tetrahydropyrazolo[3,4-c]pyridine-6-carboxylate (Intermediate J)
  • Step 1 preparation of tert -butyl 3-methyl-2,4,6,7-tetrahydropyrazolo[4,3-c]pyridine-5-carboxylate
  • Step 1 preparation of tert -butyl 1-(1-benzyloxycarbonylazetidin-3-yl)-6,7-dihydro-4 H -pyrazolo[4,3-c]pyridine-5-carboxylate (compound 1c) and tert -butyl 2-(1-benzyloxycarbonylazetidin-3-yl)-6,7-dihydro-4 H -pyrazolo[4,3-c]pyridine-5-carboxylate (compound 2c)
  • benzyl 3-iodoazetidine-1-carboxylate (compound 1b , CAS: 939759-26-9 , Vendor: PharmaBlock, 284 mg, 896 ⁇ mol) was added and the mixture was stirred at 60 °C for 2 hrs. After being cooled down, the reaction was quenched by addition of water (10 mL).
  • Step 2 preparation of tert -butyl 1-(azetidin-3-yl)-6,7-dihydro-4 H -pyrazolo[4,3-c]pyridine-5-carboxylate (compound 1d) and tert -butyl 2-(azetidin-3-yl)-6,7-dihydro-4 H- pyrazolo[4,3-c]pyridine-5-carboxylate (compound 2d)
  • Step 3 preparation of 5-[(2 R ,6 S )-2-methyl-6-[[3-(4,5,6,7-tetrahydropyrazolo[4,3-c]pyridin-1-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile (Example 1) and 5-[(2 R ,6 S )-2-methyl-6-[[3-(4,5,6,7-tetrahydropyrazolo[4,3-c]pyridin-2-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile (Example 2)
  • Example 1 slower eluting, 16 mg
  • Example 2 faster eluting, 21 mg
  • Example 1 MS: calc'd 444 (MH + ), measured 444 (MH + ).
  • Example 2 MS: calc'd 444 (MH + ), measured 444 (MH + ).
  • Example 3 and Example 4 were separated by prep-HPLC (Gradient: 20-65% ACN in Water (0.1% NH 3 ), Column: Waters X-Bridge C18, 30 ⁇ 100mm, 5 ⁇ m). Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • Step 1 preparation of 5-[(2 R ,6 S )-2-methyl-6-[[3-(6-methyl-5,7-dihydro-4 H- pyrazolo[3,4-c]pyridin-2-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile (Example 5)
  • Example 6 and Example 7 were separated by prep-HPLC (Gradient: 5-25% ACN in Water (0.1% FA), Column: Waters CSH C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 8 and Example 9 were separated by prep-HPLC (Gradient: 5-25% ACN in Water (0.1% FA), Column: Waters CSH C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 10 and Example 11 were separated by prep-HPLC (Gradient: 20-65% ACN in Water (0.1% NH 3 ), Column: Waters X-Bridge C18, 30 ⁇ 100mm, 5 ⁇ m). Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • Example 12 and Example 13 were separated by prep-HPLC (Gradient: 10-25% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m). Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • Example 14 and Example 15 were separated by prep-HPLC (Gradient: 10-30% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 16 and Example 17 were separated by chiral SFC (Gradient: 45% Isopropanol (0.1% NH 3 H 2 O) in CO 2 , Column: Daicel chiralcel OD 250 ⁇ 30mm, 5 ⁇ m). Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • Example 18 and Example 19 were separated by prep-HPLC (Gradient: 5-25% ACN in Water (0.1% TFA), Column: Waters X-Bridge C8, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 20 and Example 21 were separated by prep-HPLC (Gradient: 10-25% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 20 (slower eluting, 29 mg) was obtained as a yellow solid.
  • Example 22 and Example 23 were separated by prep-HPLC (Gradient: 10-25% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 24 (108 mg) was obtained as a light yellow solid. MS: calc'd 472 (MH + ), measured 472 (MH + ).
  • Example 25 and Example 26 were prepared in analogy to the preparation of Example 17 by using Intermediate E instead of Intermediate F.
  • Example 25 and Example 26 were separated by chiral SFC (Gradient: 50% Isopropanol (0.1% NH 3 H 2 O) in CO 2 , Column: ID, 250 ⁇ 20 mm I.D., 5 ⁇ m).
  • Example 27 and Example 28 were prepared in analogy to the preparation of Example 16 and Example 17 by using Intermediate C instead of Intermediate F.
  • the mixture of Example 27 and Example 28 and the mixture of Example 29 and Example 30 were separated by flash column in step 1.
  • Example 27 and Example 28 were separated by chiral SFC (Gradient: 35% Isopropanol (0.1% NH 3 H 2 O) in CO 2 , Column: AS, 250 ⁇ 20 mm I.D., 5 ⁇ m).
  • Example 29 and Example 30 were separated by chiral SFC (Gradient: 40% Isopropanol (0.1% NH 3 H 2 O) in CO 2 , Column: Daicel chiralcel AD 250 ⁇ 30mm, 5 ⁇ m).
  • Example 31 and Example 32 were prepared in analogy to the preparation of Example 16 and Example 17 by using Intermediate D instead of Intermediate F.
  • the mixture of Example 31 and Example 32 (slower eluting) and the mixture of Example 33 and Example 34 (faster eluting) were separated by prep-HPLC (Gradient: 10-30% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 31 and Example 32 were separated by chiral SFC (Gradient: 30% Methanol (0.1% NH 3 H 2 O) in CO 2 , Column: AD, 250 ⁇ 20 mm I.D., 5 ⁇ m).
  • Example 33 and Example 34 were separated by chiral SFC (Gradient: 45% Isopropanol (0.1% NH 3 H 2 O) in CO 2 , Column: ID, 250 ⁇ 20 mm I.D., 5 ⁇ m).
  • Example 35 and Example 36 were separated by prep-HPLC (Gradient: 25-70% ACN in Water (0.1% NH 3 ), Column: Waters RPC18, 30 ⁇ 100mm, 5 ⁇ m).
  • Example 1 The title compounds were prepared in analogy to the preparation of Example 1 and Example 2 by using tert -butyl 3-(trifluoromethyl)-2,4,5,7-tetrahydropyrazolo[3,4-c]pyridine-6-carboxylate (CAS: 733757-89-6 , Vendor: Shuya) instead of tert-butyl 2,4,6,7-tetrahydropyrazolo[4,3-c]pyridine-5-carboxylate (compound 1a ).
  • Example 37 and Example 38 were separated by prep-HPLC (Gradient: 15-35% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • Example 37 (slower eluting, 57 mg) was obtained as a yellow solid.
  • Example 42 and Example 43 were separated by prep-HPLC (Gradient: 10-30% ACN in Water (0.1% TFA), Column: Waters SunFire C18, 30 ⁇ 100mm, 5 ⁇ m).Similar to Example 1 and Example 2, the two products were also confirmed by NOESY.
  • a stable HEK293-Blue-hTLR-7 cell line was purchased from InvivoGen (Cat.#: hkb-htlr7, San Diego, California, USA). These cells were originally designed for studying the stimulation of human TLR7 by monitoring the activation of NF- ⁇ B.
  • a SEAP (secreted embryonic alkaline phosphatase) reporter gene was placed under the control of the IFN- ⁇ minimal promoter fused to five NF- ⁇ B and AP-1-binding sites. The SEAP was induced by activating NF- ⁇ B and AP-1 via stimulating HEK-Blue hTLR7 cells with TLR7 ligands.
  • the reporter expression was declined by TLR7 antagonist under the stimulation of a ligand, such as R848 (Resiquimod), for incubation of 20 hrs.
  • a ligand such as R848 (Resiquimod)
  • the cell culture supernatant SEAP reporter activity was determined using QUANTI-Blue TM kit (Cat.#: rep-qb1, Invivogen, San Diego, Ca, USA) at a wavelength of 640 nm, a detection medium that turns purple or blue in the presence of alkaline phosphatase.
  • HEK293-Blue-hTLR7 cells were incubated at a density of 250,000-450,000 cells/mL in a volume of 170 ⁇ L in a 96-well plate in Dulbecco's Modified Eagle's medium (DMEM) containing 4.5 g/L glucose, 50 U/mL penicillin, 50 mg/mL streptomycin, 100 mg/mL Normocin, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum with addition of 20 ⁇ L test compound in a serial dilution in the presence of final DMSO at 1% and 10 ⁇ L of 20uM R848 in above DMEM, perform incubation under 37 °C in a CO 2 incubator for 20 hrs.
  • DMEM Dulbecco's Modified Eagle's medium
  • a stable HEK293-Blue-hTLR-8 cell line was purchased from InvivoGen (Cat.#: hkb-htlr8, San Diego, California, USA). These cells were originally designed for studying the stimulation of human TLR8 by monitoring the activation of NF- ⁇ B.
  • a SEAP (secreted embryonic alkaline phosphatase) reporter gene was placed under the control of the IFN- ⁇ minimal promoter fused to five NF- ⁇ B and AP-1-binding sites. The SEAP was induced by activating NF- ⁇ B and AP-1 via stimulating HEK-Blue hTLR8 cells with TLR8 ligands.
  • the reporter expression was declined by TLR8 antagonist under the stimulation of a ligand, such as R848, for incubation of 20 hrs.
  • the cell culture supernatant SEAP reporter activity was determined using QUANTI-Blue TM kit (Cat.#: rep-qb1, Invivogen, San Diego, Ca, USA) at a wavelength of 640 nm, a detection medium that turns purple or blue in the presence of alkaline phosphatase.
  • HEK293-Blue-hTLR8 cells were incubated at a density of 250,000-450,000 cells/mL in a volume of 170 ⁇ L in a 96-well plate in Dulbecco's Modified Eagle's medium (DMEM) containing 4.5 g/L glucose, 50 U/mL penicillin, 50 mg/mL streptomycin, 100 mg/mL Normocin, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum with addition of 20 ⁇ L test compound in a serial dilution in the presence of final DMSO at 1% and 10 ⁇ L of 60uM R848 in above DMEM, perform incubation under 37 °C in a CO 2 incubator for 20 hrs.
  • DMEM Dulbecco's Modified Eagle's medium
  • a stable HEK293-Blue-hTLR-9 cell line was purchased from InvivoGen (Cat.#: hkb-htlr9, San Diego, California, USA). These cells were originally designed for studying the stimulation of human TLR9 by monitoring the activation of NF- ⁇ B.
  • a SEAP (secreted embryonic alkaline phosphatase) reporter gene was placed under the control of the IFN- ⁇ minimal promoter fused to five NF- ⁇ B and AP-1-binding sites. The SEAP was induced by activating NF- ⁇ B and AP-1 via stimulating HEK-Blue hTLR9 cells with TLR9 ligands.
  • TLR9 antagonist under the stimulation of a ligand, such as ODN2006 (Cat.#: tlrl-2006-1, Invivogen, San Diego, California, USA), for incubation of 20 hrs.
  • a ligand such as ODN2006 (Cat.#: tlrl-2006-1, Invivogen, San Diego, California, USA)
  • the cell culture supernatant SEAP reporter activity was determined using QUANTI-Blue TM kit (Cat.#: rep-qb1, Invivogen, San Diego, California, USA) at a wavelength of 640 nm, a detection medium that turns purple or blue in the presence of alkaline phosphatase.
  • HEK293-Blue-hTLR9 cells were incubated at a density of 250,000-450,000 cells/mL in a volume of 170 ⁇ L in a 96-well plate in Dulbecco's Modified Eagle's medium (DMEM) containing 4.5 g/L glucose, 50 U/mL penicillin, 50 mg/mL streptomycin, 100 mg/mL Normocin, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum with addition of 20 ⁇ L test compound in a serial dilution in the presence of final DMSO at 1% and 10 ⁇ L of 20uM ODN2006 in above DMEM, perform incubation under 37 °C in a CO 2 incubator for 20 hrs.
  • DMEM Dulbecco's Modified Eagle's medium
  • the compounds of formula (I) or (Ia) have human TLR7 and/or TLR8 inhibitory activities (IC 50 value) ⁇ 0.5 ⁇ M. Moreover, some compounds also have human TLR9 inhibitory activity ⁇ 0.5 ⁇ M. Activity data of the compounds of the present invention were shown in Table 2. Table 2.
  • the hERG channel inhibition assay is a highly sensitive measurement that identifies compounds exhibiting hERG inhibition related to cardiotoxicity in vivo.
  • the hERG K + channels were cloned in humans and stably expressed in a CHO (Chinese hamster ovary) cell line.
  • CHO hERG cells were used for patch-clamp (voltage-clamp, whole-cell) experiments. Cells were stimulated by a voltage pattern to activate hERG channels and conduct I KhERG currents (rapid delayed outward rectifier potassium current of the hERG channel). After the cells were stabilized for a few minutes, the amplitude and kinetics of I KhERG were recorded at a stimulation frequency of 0.1 Hz (6 bpm).
  • test compound was added to the preparation at increasing concentrations. For each concentration, an attempt was made to reach a steady-state effect, usually, this was achieved within 3-10 min at which time the next highest concentration was applied.
  • the amplitude and kinetics of I KhERG are recorded in each concentration of the drug which were compared to the control values (taken as 100%). (references: Redfern WS, Carlsson L, Davis AS, Lynch WG, MacKenzie I, Palethorpe S, Siegl PK, Strang I, Sullivan AT, Wallis R, Camm AJ, Hammond TG.
  • results of hERG are given in Table 3.
  • a safety ratio (hERG IC 20 /EC 50 ) > 30 suggests a sufficient window to differentiate the pharmacology by inhibiting TLR7/8/9 pathways from the potential hERG related cardiotoxicity.
  • hERG IC 20 / TLR7/8/9 IC 50 which serves as early selectivity index to assess hERG liability, obviously reference compounds ER-887258, ER-888285, ER-888286, R1 and R2 have much narrower safety window compared to the compounds of this invention.
  • hERG and safety ratio results Example No hERG IC 20 ( ⁇ M) hERG IC 50 ( ⁇ M) hERG IC 20 / TLR7 IC 50 hERG IC 20 / TLR8 IC 50 hERG IC 20 / TLR9 IC 50 ER-887258 0.687 2.784 8.1 N.A. 0.3 ER-888285 1.006 3.105 8.4 N.A. 0.5 ER-888286 0.348 1.297 0.3 N.A.
  • the compounds would be desirable to have minimal DDI (drug-drug interaction) liabilities. Therefore, the effects of compounds of formula (I) or (Ia) on CYP2D6 are determined.
  • the assay plate then was placed back in the 37 °C incubator. After 10 minutes incubation, incubates were quenched by addition of 50 ⁇ L 100% acetonitrile containing internal standards (20 ng/mL D3-Dextrorphan). The supernatants were collected for RapidFire/MS/MS analysis.
  • RapidFire online solid phase extraction/sample injection system (Agilent) coupled with API4000 triple quadrupole mass spectrometer (AB Sciex) were used for sample analysis.
  • the mobile phase composed of acetonitrile and water supplemented with 0.1% formic acid.
  • a C4 solid phase extraction cartridge is used for sample separation. MS detection is achieved in positive ion MRM mode.
  • Peak areas for substrate, metabolite and internal standard are determined using the RapidFire integrator software (version 3.6.12009.12296). Peak area ratios (PAR) of metabolite and internal standard (stable-labelled metabolite) are then calculated. The measurement window for each experiment is then defined:
  • the compounds of present invention were found to have low CYP inhibition for CYP2D6 determined in the assays described above. Table 5. CYP inhibition of the compounds of this invention for CYP2D6 Example No CYP 2D6 inhibition % @ 10 ⁇ M ER-888286 52.5 2 1.0 3 10.5 4 7.5 5 -1.5 6 8.5 7 -19.5 8 11.0 9 15.0 11 -6.0 12 6.0 13 3.0 15 -10.5 16 8.0 17 1.5 18 -2.5 19 -2.5 20 -6.0 21 10.5 22 5.5 23 3.0 26 20.0 27 22.0 28 0.5 30 3.5 31 -3.5 32 11.0 34 -11.5 36 20.3 42 17.5 percentage inhibition ⁇ 0: not or weak inhibitor
  • human peripheral blood mononuclear cell represents primary human immune cells in blood mainly consisting of lymphocytes, monocytes, and dendritic cells. These cells express TLR7, TLR8, or TLR9, and therefore are natural responders to respective ligand stimulation.
  • TLR7, TLR8, or TLR9 Upon activation of these TLRs, PBMCs secrete similar cytokines and chemokines in vitro and in vivo, and therefore the in vitro potency of a TLR7/8/9 antagonist in human PBMC is readily translatable to its pharmacodynamics response in vivo.
  • PBMC Human peripheral blood mononuclear cells
  • PBMC PBMC were resuspended at a final concentration of 2 ⁇ 10 6 cells/mL in RPMI-1640 media with GlutaMAXTM (Gibco) supplemented with 10% Fetal Bovine Serum (Sigma) and plated at 150 ⁇ L/well (3 ⁇ 10 5 cells/well) in tissue culture treated round bottom 96-well plates (Corning Incorporated).
  • Antagonist compounds solubilized and serial diluted in 100% DMSO were added in duplicate to cells to yield a final concentration of 1% DMSO (v/v).
  • PBMC were incubated with antagonist compounds for 30 minutes at 37°C, 5% CO 2 before adding various TLR agonist reagents in 48 ⁇ L complete media per well as follows (final concentrations indicated): CpG ODN 2216 (InvivoGen) at 1 ⁇ M for TLR9, ORN 06/LyoVec (InvivoGen) at 1 ⁇ g/mL for TLR8 and R848 (InvivoGen) at 1 ⁇ g/mL for TLR7 and TLR8. PBMC were incubated overnight at 37°C with 5% CO 2 .
  • Luminex assay ProcartaPlexTM Multiplex Immunoassay, Invitrogen
  • ELISA procedure according to the manufacturer's recommended protocol (eBioscience, ThermoFisher Scientific). Viability of the cells was also checked with Cell Viability Assay (CellTiter Glo ® Luminescent Cell Viability Assay, Promega). Table 6.
  • hPBMC results Example No hPBMC/TLR9 IC 50 ( ⁇ M) ER-888286 1.629 3 0.118 4 0.198 5 0.318 7 0.195 12 0.092 13 0.119 15 0.110 16 0.084 17 0.113 20 0.097 21 0.092 23 0.143 25 0.346 27 0.081 28 0.214 29 0.115 30 0.162 31 0.235 32 0.145 33 0.072 34 0.202
  • the human microsomal stability assay is used for early assessment of metabolic stability of a test compound in human liver microsomes.
  • Human liver microsomes (Cat.NO.: 452117, Corning, USA;Cat.NO.:H2610, Xenotech, USA) were preincubated with test compound for 10 minutes at 37°C in 100 mM potassium phosphate buffer, pH 7.4. The reactions were initiated by adding NADPH regenerating system. The final incubation mixtures contained 1 ⁇ M test compound, 0.5 mg/mL liver microsomal protein, 1 mM MgCl 2 , 1 mM NADP, 1 unit/mL isocitric dehydrogenase and 6 mM isocitric acid in 100 mM potassium phosphate buffer, pH 7.4.

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EP19805549.3A 2018-11-09 2019-11-07 5-[6-[[3-(4,5,6,7-tetrahydropyrazolo[4,3-c]pyridin-1-yl)azetidin-1-yl]methyl]morpholin-4-yl]quinoline-8-carbonitrile derivatives and similar compounds as tlr7-9 antagonists for treating systemic lupus erythematosus Active EP3877388B1 (en)

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