EP3791158A1 - Transmissionsvorrichtung und verfahren zum untersuchen von wenigstens einer probe in einer mikrotiterplatte mittels transmission - Google Patents
Transmissionsvorrichtung und verfahren zum untersuchen von wenigstens einer probe in einer mikrotiterplatte mittels transmissionInfo
- Publication number
- EP3791158A1 EP3791158A1 EP19720549.5A EP19720549A EP3791158A1 EP 3791158 A1 EP3791158 A1 EP 3791158A1 EP 19720549 A EP19720549 A EP 19720549A EP 3791158 A1 EP3791158 A1 EP 3791158A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- light
- emission
- emission light
- detector
- microtiter plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0407—Batch operation; multisample devices with multiple optical units, e.g. one per sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0826—Fibre array at source, distributing
Definitions
- the invention relates to a transmission device for examining at least one sample in a microtiter plate, comprising a lighting device and a detection device, between which a gap is formed, which is adapted to receive a microtiter plate, wherein the illumination device at least one Emissive source and the lighting device is adapted to guide generated by the emission source emission light through the gap, wherein the detection device has at least one detector which is adapted to measure from the gap received light signals.
- the invention further relates to a method for examining at least one sample in a microtiter plate by means of transmission.
- microtiter plates are often used in the medical, biological and chemical fields. Such microtiter plates have a number of wells or cavities in which the samples are placed.
- the dimensions of the microtiter plates are standardized according to the ANSI standard. There are also various formats which have a different number of cavities, for example twelve, forty-eight, ninety-six, three-four-eighty and thousand-five hundred and thirty-six.
- a commonly used assay procedure for specimens in microtiter plates is the transmission study, in which light is passed through the cavities and the samples contained therein and the transmitted light is measured. In this way, information about properties of the samples can be obtained.
- enzyme-linked immunosorbent assay (ELISA) studies often employ a transmission method.
- ELISA studies antigens are detected by absorptively binding the antigens via a first antibody, and an enzyme-coupled secondary antibody results in a reaction of a dye substrate. This reaction of the dye substrate can be detected by ELISA.
- these devices often provide shielding of the measuring space in which the microtiter plate is arranged during the measurement. As a result, the detector of the device is protected against the ingress of stray light. Disadvantageously, however, this shielding also takes up installation space, so that these devices have correspondingly large dimensions.
- the object of the invention is to provide a transmission device for examining at least one sample in a microtiter plate and a method for examining at least one sample in a microtiter plate by means of transmission with which these investigations can be performed simply, cost-effectively and / or. or can be carried out in a space-saving manner.
- a transmission device for examining at least one sample in a microtiter plate comprising an illumination device and a detection device, between which a gap is formed, which is adapted to receive a microtiter plate, wherein the illumination device at least one Emission source and the illumination device is designed to guide emission light generated by the emission source through the gap, wherein the detection device has at least one detector, which is designed to receive from the intermediate space.
- the transmission device is further developed in that the detection device comprises an angle-dependent filter which is arranged between the illumination device and the at least one detector in the beam path of the emission light and transmits essentially only such light rays whose angles of incidence are smaller than a predefinable critical angle.
- the angle of incidence and the critical angle are each defined as an angle to the beam path of the emission light in the intermediate space.
- An incidence of light rays along this ray path is called a perpendicular incidence in the angle-dependent filter.
- light signals are to be understood as those light beams which pass from the intermediate space to the detector.
- Lichtsig signals which are due to a reaction of the at least one sample with the emission light, for example due to the reaction of a dye substrate or by fluorescence.
- light beams of the scattered light are included, which reach the at least one detector and can be detected by the detector.
- the critical angle is set in such a way that, on the one hand, the light signals, which are due to a reaction of the at least one sample with the emission light, can pass through the filter unhindered, but on the other hand substantially completely blocks incident stray light.
- the critical angle is specified in particular to a value between 30 ° and 1, more particularly to a value between 10 ° and 5 °.
- the at least one detector is in particular a photodiode which is sensitive in the wavelength range of the emission light or the wavelength of the light signals which are attributable to a reaction of the at least one sample with the emission light.
- the transmitted light passes exclusively through the angle-dependent filter and optionally a lens to the at least one detector.
- Another light guide is thus unnecessary in the detection device, whereby the equipment cost is significantly reduced.
- the angle-dependent filter is designed as a film.
- a normal of the film plane is parallel to the beam path of the emission light in the intermediate space.
- the angle-dependent filter is a component of the detection device, which in particular closes off the intermediate space or defines it to one side.
- the angle-dependent filter is preferably used as a blinding filter film. ter formed with mutually parallel slats.
- Such privacy filters consist of a large number of these slats, which have a very small width in the direction of a perpendicular to the filter, so that vertically incident light passes through the filter almost unhindered.
- the height of the lamellae ie the extent of the lamellae in the direction of the vertical, and the distance of the lamellae from one another define a critical angle in the transverse direction of the lamellae. Beams whose angle of incidence is above the critical angle are blocked.
- the gap is closed in the longitudinal direction of the slats.
- stray light can not enter the privacy film in the longitudinal direction of the slats.
- angle dependent filters may be used, such as e.g. Interference filter.
- the critical angle is wavelength-dependent
- the interference filter is designed such that the critical angle in the entire wavelength range of the at least one detector lies in the range between 30 ° and 1 °, in particular between 10 ° and 1 ° ,
- the intermediate space is formed as a rectangular opening in the transmission device, so that the transmission device is designed as an open measurement setup and the microtiter plate that can be introduced or located in the intermediate space is accessible without the need for confirmation of a closure element.
- an open measuring structure is understood to mean that the intermediate space is provided as an opening and is connected to a closure element, for example a closure element. flap or a shutter, is omitted for this opening.
- the design of the transmission device as an open measurement setup advantageously allows a space-saving design. Since incident stray light is blocked by the angle-dependent filter, a closure element is superfluous.
- a microtiter plate can be inserted at any time and removed at any time through the open measuring setup, whereby a quick and easy handling of the transmission device is achieved.
- the intermediate space is preferably substantially complementary in shape to the microtiter plate which can be introduced or located in the intermediate space.
- the intermediate space is designed such that a microtiter plate can be received in an exact fit, so that the dimensions of the transmission device are kept small.
- the illumination device is set up to divide the emission light generated by the emission source into a plurality of partial beam paths, wherein a plurality of the partial beam paths run as transmission beam paths through the gap to a respective detector unit of the detection device, each detector unit each comprising at least one detector.
- the transmission beam paths are in the intermediate space in particular parallel to each other.
- the transmission device in this way, for example, to Examination of microtiter plates with twelve, forty eight, ninety six, or three hundred eighty four wells.
- the distribution of the emission light to a plurality of transmission beam paths realizes a compact and cost-effective design of the transmission device.
- the detector units comprise, for example, a plurality of photodiodes, each of which is sensitive in different wavelength ranges, so that light signals can be detected at different wavelengths. This provides a compact and at the same time flexible transmission device.
- At least one of the partial beam paths is a reference beam path, which is set up to guide the emission light to a reference detector unit which is arranged in the illumination device.
- the reference beam path is a partial beam path which is formed in addition to the transmission beam paths passing through the gap.
- the illumination device preferably comprises a light mixer, which is designed to homogenize the emission light generated by the emission source and to distribute it with uniform intensity to the partial beam paths, wherein in particular the light mixer has a rectangular cross section.
- the light mixer is, for example, an elongated body with a rectangular cross section, in which the emission light of the emission source is homogenized.
- the partial beam paths in the illumination device extend in each case into a light conductor which is bundled with its entrance sides against the light mixer, wherein the light guides, in which the transmission beam paths extend, are arranged to occupy a portion of the emission - Onslichts of the light mixer to each lead to an emission opening of the illumination device, in particular the emission openings are formed as recesses in a holding plate, wherein in particular in the emission openings spherical lenses are arranged.
- the light guides are flexible cables, such as fiber optic cables or polymeric optical fibers. These light guides are bundled at their entrance side to the light mixer, so that the emission light is transmitted evenly to all optical fibers.
- the exit sides of the light guides of the transmission beam passages are located at the emission openings.
- These emission openings are preferably arranged centrally above the cavities, so that the emission light is conducted through the light guides and enters the cavities from the emission openings.
- ball lenses are preferably provided in the emission openings.
- the emission source comprises at least two, in particular at least three, in particular at least four light-emitting diodes, wherein the emission light of the light-emitting diodes is combined in the light mixer, where an interference filter is arranged between each light-emitting diode and the light mixer, wherein in particular a ball lens is arranged in front of and in particular a further ball lens behind each interference filter, wherein in particular a first light-emitting diode for emitting emission light having a wavelength of 405 nm, a second light-emitting diode for emitting emission light having a wavelength of 450 nm, a third light-emitting diode for emitting emission light having a wavelength of 540 nm and a fourth light-emitting diode for emission of emission light with a wavelength of 630 nm.
- the interference filters limit the spectra of the wavelengths of the emission light of the light-emitting diodes, so that the emission spectra are each narrow-band.
- the ball lenses which are arranged between the LEDs and the interference filters, the emission light is parallelized before entering the interference filter.
- the spherical lenses arranged between the interference filters and the light mixer couple the light into the light mixer.
- the transmission device can be used to carry out different tests on the samples in the microtiter plate without the need for a further transmission device or for the LEDs to be replaced.
- the LEDs are arranged horizontally next to one another.
- the light mixer preferably has, for each light-emitting diode, a separate arm, which together in the direction of propagation of the light. men convinced.
- the base of the light mixer has a substantially triangular shape, in which case one side of the triangle is provided for coupling the emission light and the two other sides converge in the propagation direction of the light.
- the transmission device comprises status lights, which are arranged on the outside of the transmission device and light up when the light emitting diodes light. In particular, a portion of the emission light of each light emitting diode is used to illuminate each one status light.
- the object is further achieved by a method for examining at least one sample in a microtiter plate by means of transmission, wherein emission light during a first period in a lighting device by means of an emission source he testifies and the emission light through at least one cavity of the microtiter plate in which the at least one sample is passed, wherein light signals received from the at least one cavity are measured during the first period by means of at least one detector arranged in a detection device, wherein the method is further developed by the at least one detector being developed by means of a angle-dependent filter is protected against the onset of stray light, wherein the angle-dependent filter between the illumination device and the at least one detector in the beam path of the emission light is arranged and essentially only passes such light rays, whose angles of incidence are smaller than a predefinable critical angle.
- the at least one sample is examined with an open measurement setup.
- the microtiter plate is thus arranged in a space designed as an opening, which is not closed during the examination with a closure element.
- the light signals measured during the first period represent a light measurement, wherein during a second period of time no emission light is passed through the at least one cavity and the light signals measured during the second period represent a dark measurement, the dark measurement of the light measurement is subtracted.
- the light measurement is performed during the first period and the dark measurement is performed during the second period.
- the microtiter plate is arranged in the intermediate space both during the first time period and during the second time period. Since no emission light is passed through the cavities during the second period, the light signals measured during the second period correspond to a background caused, for example, by stray light. By subtracting the dark measurement from the light measurement, the background is thus removed from the measurements and the quality of the examinations increased.
- the first period and the second period are the same length. In this way, the dark measurement can be subtracted from the light measurement without further conversion.
- measuring cycles are run through, at least one light measurement and at least one dark measurement are carried out in each measurement cycle, and a dark measurement measured in the same measurement cycle is subtracted from each light measurement measured in a measurement cycle.
- a measurement cycle consists of a single light measurement and a single dark measurement, wherein the first period and the second period are each 5 ms.
- This measuring cycle is repeated many times, whereby in each case the dark signal measured in a measuring cycle is subtracted from the light signal measured in the same measuring cycle.
- the duration of a measurement cycle is as small as possible, in particular between 5 ms and 50 ms, so that even high-frequency changes in the scattered light incidence can be taken into account in the measurement.
- the emission light is split and at the same time passed through a plurality of cavities of the microtiter plate, the light signals of each cavity being measured by one detector unit each having at least one detector.
- the light measurements and the dark measurements are advantageously measured separately for all cavities.
- different scattered light intensities are taken into account.
- Detector units which are arranged closer to the opening of the transmission device, are exposed to increased stray light.
- the separate light measurements and dark measurements for each cavity take account of the different scattered light conditions at the location of the different cavities or detector units.
- the emission light is generated with at least two, in particular at least three, in particular at least four different wavelengths by means of a respective light emitting diode of the emission source, wherein the bandwidth of the emission light of each light emitting diode is limited by means of an interference filter, in particular a first light emitting diode emission light with a wavelength of 405 nm, a second light emitting diode emitting light having a wavelength of 450 nm, a third light emitting diode emitting light having a wavelength of 540 nm and a fourth light emitting light having a wavelength of 630 nm.
- an interference filter in particular a first light emitting diode emission light with a wavelength of 405 nm, a second light emitting diode emitting light having a wavelength of 450 nm, a third light emitting diode emitting light having a wavelength of 540 nm and a fourth light emitting light having a wavelength of 630 nm.
- the examination of the at least one sample is performed sequentially for each wavelength. For example, it is provided that first a light measurement with a first wavelength is measured, then a dark measurement is performed. This is repeated for each wavelength, so that at four wavelengths a total of eight measurements are performed, which together form one measurement cycle. It is likewise possible for the light measurements with different wavelengths to be carried out one after the other and then a single dark measurement is carried out so that the four light measurements and the dark measurement form one measurement cycle.
- a measuring cycle with the the first wavelength and a dark measurement is repeated many times and then the measuring cycles with the other wavelengths and each one dark measurement are repeated many times.
- all these methods are used to investigate at least one sample having different wavelengths, while at the same time minimizing the influence of scattered light on the examination.
- an aging of the emission source and / or a change in the intensity of the emission light of the emission source is measured by means of a reference measurement, wherein the emission light is conducted via a reference beam path to a reference detector unit arranged in the illumination device which measures the intensity of the emission light detects where the intensity of the emission light is compared with previously measured and / or predefined values for the intensity of the emission light.
- Such a reference measurement can be carried out, for example, before and / or after the examination of the samples, in order to monitor the aging of the light-emitting diodes and to check the quality of the examination.
- Embodiments of the invention may be individual features or a Combine several features.
- 1 is a schematic representation of a transmission device for examining at least one sample in a microtiter plate
- FIG. 2 shows a schematic representation of a microtiter plate with ninety-six cavities
- FIG. 3 is a schematic representation of a lighting device
- FIG. 4 is a schematic representation of the internal structure of a lighting device comprising an emission source
- Fig. 5 shows the illustration of Fig. 4 with additionally shown
- FIG. 6 is a schematic representation of a detection device
- Fig. 8 is a representation of the operation of a privacy filter.
- the same or similar elements and / or parts are provided with the same reference numerals, so that apart from a new idea each.
- the transmission device 1 schematically shows an exemplary embodiment of a transmission device 1.
- the transmission device 1 comprises an illumination device 2 and a detection device 4, between which there is an intermediate space 6 designed as a rectangular opening.
- the intermediate space 6 is designed such that a microtiter plate 8, as shown in FIG. 2, can be inserted with an exact fit.
- the dimensions of the intermediate space 6 therefore essentially correspond to the dimensions of the microtiter plate 8, as a result of which the transmission device 1 has a compact construction.
- the transmission device 1 comprises a plurality of status lamps 3. These status lamps 3 are each assigned to a light-emitting diode arranged in the lighting device 2, which are concealed in FIG. 1. If one of the light emitting diodes emits light, the associated status light 3 also lights up. For reasons of clarity, only one of the status lights 3 is provided with a reference symbol.
- the microtiter plate 8 shown by way of example in FIG. 2 is a format with ninety-six cavities 80, of which in turn only one cavity 80 is provided with a reference numeral. In these cavities 80, the samples to be examined are arranged before the microtiter plate 8 is inserted into the intermediate space 6. Since the dimensions of microtiter plates 8 satisfy an ANSI standard, the gap 6 can be designed in a shape-complementary manner to these dimensions.
- FIG. 3 shows a schematic representation of the illumination device. tion 2, wherein in Fig. 3, a representation was selected from an angle from obliquely below.
- the illumination device 2 has an ejection device 29, with which the microtiter plate 8 can be ejected quickly and easily from the intermediate space 6.
- a holding plate 28 is arranged, which has a number of emission openings 27, of which only one is provided with a reference numeral.
- the number of emission openings 27 corresponds to the number of cavities 80 of the microtiter plate 8.
- the emission openings 27 are arranged such that in the inserted state of the microtiter plate 8, each emission opening 27 is arranged centrally above a cavity 80.
- the internal structure of the illumination device 2 is shown in FIGS. 4 and 5.
- the view chosen in FIGS. 4 and 5 corresponds to the view in FIG. 1, so that the lower side of the holding plate 28 concealed in FIGS. 4 and 5 corresponds to the underside of the holding plate 28 shown in FIG.
- the illumination device 2 has an emission source 20 which, in the example shown in FIG. 4, comprises four light-emitting diodes 21 a, 21 b, 21 c, 21 d.
- the emission light of the light emitting diode 21a has a wavelength of 405 nm
- the emission light of the light emitting diode 21b has a wavelength of 450 nm
- the emission light of the light emitting diode 21c has a wavelength of 540 nm
- the emission light of the light emitting diode 21d has a wave length of 630
- a spherical lens 23 is arranged in each case, which parallels the emerging emission light , For reasons of clarity again only one of the ball lenses 23 is provided with a reference numeral. Behind everyone Ball lens 23, an interference filter 22 is arranged in each case, which restricts the wavelength spectrum of the emission light of the light emitting diodes 21 a to 21 d. According to another embodiment, not shown in FIG. 4, behind each interference filter 22, another ball lens is arranged, which focuses the emission light.
- a light mixer 24 is arranged.
- This light mixer 24 homogenizes the incident emission light, so that it distributes itself with a uniform intensity in the cross section of the light mixer 24.
- the light mixer 24 according to the embodiment shown in FIG. 4 advantageously has a rectangular cross-section. If only a single light emitting diode 21 a is provided, the light mixer 24 has, for example, the shape of a rod with a rectangular cross section. If, however, a plurality of light emitting diodes 21 a to 21 d provided, as shown in Fig. 4, the light mixer 24, the emission light of the LEDs 21 a to 21 d together. This can, for example, as shown in Fig. 4, happen by means of four arms together.
- the light mixer 24 may have a substantially triangular base area in which, compared to the embodiment shown in Fig. 4, the area between the arms is filled.
- partial beam paths 25 are schematically illustrated, to which the light emerging from the light guide 24 emission light of the light emitting diodes 21 a to 21 d is divided.
- a bundle of light guides 26 is arranged at the output of the light mixer 24, in each of which a portion of the emission light is coupled in uniformly. From the output of the light mixer 24, these light guides 26 each lead to an emission opening 27, in which arranged for further focusing of the emission light in each case a ball lens, not shown is.
- Those partial beam paths 25 that run in the light guides 26 to the emission openings 27 are transmission beam paths.
- a further light guide 26 leads as a reference beam path 30 back to a reference detector unit 32, which is arranged next to the light emitting diodes 21 a to 21 d.
- FIG. 6 schematically shows a detection device 4 which is arranged below the illumination device 2 and the inserted microtiter plate 8.
- the detection device 4 has an angle-dependent filter 42, which in the illustration in FIG. 6 is designed as a foil.
- This angle-dependent filter 42 is designed in such a way that it transmits essentially only those light beams whose angles of incidence are smaller than a predefinable critical angle.
- the critical angle is related to the transmission beam paths of the emission light in the intermediate space 6, which corresponds to a perpendicular to the angle-dependent filter 42. In this way it is prevented that stray light, which obliquely enters the gap 6, can pass through the angle-dependent filter 42, so that only the light signals of the samples pass from the gap 6.
- FIG. 7 shows an exploded view of the detection device 4 from FIG. 6.
- FIG. 7 shows that below the angle-dependent filter 42 a detector plate 49 is arranged, which has a row of detector openings 41, each centrally located below the detector Emission openings 27 and the cavities 80 are arranged.
- a detector unit In each of these detector openings 41 is a detector unit with each arranged at least one detector 40, which are hidden by the perspective in Fig. 7.
- the detectors 40 are, for example, photodiodes with sensitivities in different wavelength ranges.
- ball lenses 43 are respectively arranged in the openings.
- the transmission beam paths each proceed from the light mixer 24 through a light guide 26, an emission opening 27, the intermediate space 6 or a cavity 80, the angle-dependent filter 42 to a detector 40 or a detector unit. After the exit from the emission openings 27, the transmission beam paths run parallel to one another.
- a privacy filter can be used for example as an angle-dependent filter 42.
- the privacy filter comprises a number of lamellae 44 arranged parallel to one another. If light falls along a vertical 47 in the privacy filter, it can pass through the small width of the fins 44 unhindered the privacy filter. If, however, the angle of incidence 46 of a light beam 45 in the transverse direction of the slats is greater than a critical angle 48, the light beam 45 can not pass the privacy filter.
- the privacy filter is preferably arranged in the transmission device 1 that the transverse direction of the fins 44 corresponds to the direction of the opening of the gap 6.
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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DE102018111032.4A DE102018111032A1 (de) | 2018-05-08 | 2018-05-08 | Transmissionsvorrichtung und Verfahren zum Untersuchen von wenigstens einer Probe in einer Mikrotiterplatte mittels Transmission |
PCT/EP2019/060746 WO2019214970A1 (de) | 2018-05-08 | 2019-04-26 | Transmissionsvorrichtung und verfahren zum untersuchen von wenigstens einer probe in einer mikrotiterplatte mittels transmission |
Publications (1)
Publication Number | Publication Date |
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EP3791158A1 true EP3791158A1 (de) | 2021-03-17 |
Family
ID=66334462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP19720549.5A Pending EP3791158A1 (de) | 2018-05-08 | 2019-04-26 | Transmissionsvorrichtung und verfahren zum untersuchen von wenigstens einer probe in einer mikrotiterplatte mittels transmission |
Country Status (4)
Country | Link |
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US (1) | US20210055208A1 (de) |
EP (1) | EP3791158A1 (de) |
DE (1) | DE102018111032A1 (de) |
WO (1) | WO2019214970A1 (de) |
Families Citing this family (1)
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US20220099679A1 (en) * | 2020-09-27 | 2022-03-31 | Sylvester Tumusiime | Portable diagnostic device for viewing biological entities and structures |
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JPS6040955A (ja) * | 1983-08-17 | 1985-03-04 | Japan Spectroscopic Co | 自動マイクロプレ−ト分光分析装置及び方法 |
JPS63298137A (ja) * | 1987-05-29 | 1988-12-05 | Soken:Kk | イメ−ジファイバを用いた検体分析装置 |
US5073029A (en) * | 1990-02-16 | 1991-12-17 | Eqm Research, Inc. | Multisource device for photometric analysis and associated chromogens |
US5271079A (en) * | 1991-11-08 | 1993-12-14 | Finisar Corporation | Light mixing device with fiber optic output |
JPH06324054A (ja) * | 1993-05-11 | 1994-11-25 | J T Sci:Kk | タイタプレートリーダ |
AT3085U1 (de) * | 1998-10-23 | 1999-09-27 | Tecan Austria Gmbh | Messkopf |
US6645737B2 (en) * | 2001-04-24 | 2003-11-11 | Dade Microscan Inc. | Method for maintaining test accuracy within a microbiological test array |
EP2240613B1 (de) * | 2008-02-06 | 2013-09-11 | Ludwig-Maximilians-Universität München | Thermooptische charakterisierung von nukleinsäuremolekülen |
JP2013033008A (ja) * | 2011-08-03 | 2013-02-14 | Sony Corp | 光学分析装置及び光学分析方法 |
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2018
- 2018-05-08 DE DE102018111032.4A patent/DE102018111032A1/de active Pending
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2019
- 2019-04-26 EP EP19720549.5A patent/EP3791158A1/de active Pending
- 2019-04-26 WO PCT/EP2019/060746 patent/WO2019214970A1/de unknown
-
2020
- 2020-11-06 US US17/090,966 patent/US20210055208A1/en active Pending
Also Published As
Publication number | Publication date |
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WO2019214970A1 (de) | 2019-11-14 |
US20210055208A1 (en) | 2021-02-25 |
DE102018111032A1 (de) | 2019-11-14 |
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