EP3600316A1 - Nicotinamide pour le traitement de la dyslipidémie - Google Patents

Nicotinamide pour le traitement de la dyslipidémie

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Publication number
EP3600316A1
EP3600316A1 EP18721044.8A EP18721044A EP3600316A1 EP 3600316 A1 EP3600316 A1 EP 3600316A1 EP 18721044 A EP18721044 A EP 18721044A EP 3600316 A1 EP3600316 A1 EP 3600316A1
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EP
European Patent Office
Prior art keywords
phosphate
nicotinamide
pharmaceutical preparation
levels
patients
Prior art date
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EP18721044.8A
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German (de)
English (en)
Inventor
Richard Ammer
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Salmon Pharma GmbH
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Salmon Pharma GmbH
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Publication date
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Publication of EP3600316A1 publication Critical patent/EP3600316A1/fr
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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/455—Nicotinic acids, e.g. niacin; Derivatives thereof, e.g. esters, amides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/785—Polymers containing nitrogen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A61K33/10—Carbonates; Bicarbonates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/244—Lanthanides; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/26—Iron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Definitions

  • the present invention relates to a pharmaceutical preparation comprising nicotinamide for use in a method of preventing and/or treating dyslipidemia, particularly resulting from renal failure, and a pharmaceutical preparation comprising nicotinamide for use in a method of preventing and/or treating elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, both particularly resulting from renal failure.
  • Hyperphosphatemia defined as super-physiological levels of phosphate, is considered an independent risk factor for patients in chronic kidney disease (CKD) or chronic renal failure (CRF), and adequate therapy is still a challenge for which ca. 50% to 70% of CKD patients do not meet recommended target phosphate levels [KDIGO guideline 2009 (8); K/DOQI clinical practice guidelines 2003 (7)] according to DOPPS III [Young 2004 (1 ), Tentori 2008 (2)].
  • Kidney failure is the main cause of hyperphosphatemia.
  • Chronic renal failure (CRF) is a progressive kidney disease; when the kidney has lost all its ability of clear the blood from extensive fluid volume, electrolytes, metabolic substances, the patients cannot survive and have to be referred to dialysis.
  • CRF Chronic renal failure
  • ESRD End-Stage Renal-Disease
  • One of the most crucial electrolytes is phosphate.
  • CKD disrupts systemic calcium and phosphate homeostasis and affects the bone, gut, and parathyroid glands. This occurs because of decreased renal excretion of phosphate and diminished renal hydroxylation of 25-hydroxyvitamin D to calcitriol (1 ,25 dihydroxyvitamin D) [Levin, 2007 (3)]. Progressive kidney dysfunction results in hyperphosphatemia and calcitriol deficiency. These ultimately can result in hypocalcaemia. These abnormalities directly increase PTH levels via sensing the Calcium-Sensing Receptor (CaSR) as potent stimulus to the release of PTH. I n conseq uence, hyperphosphatemia is also an important factor underlying hyperparathyroidism.
  • CaSR Calcium-Sensing Receptor
  • FGF23 a novel phosphaturic factor
  • ESRD end stage renal disease
  • Hyperphosphatemia also lowers the levels of ionized calcium and interferes with the production of 1 ,25-dihydroxyvitamin D, thereby resulting in increased PTH levels.
  • Hyperphosphatemia and secondary hyperparathyroidism with abnormalities in serum phosphate and calcium levels are associated with morbidity, renal osteodystrophy, and mortality.
  • a number of reports have delineated an increased risk of all-cause and cardiovascular mortality in patients with disorders of mineral metabolism.
  • the association with decreased survival primarily involves increased phosphate, calcium, calcium x phosphate product, and/or parathyroid hormone levels. These in turn are associated with accelerated atherosclerosis, arterial calcification, and an increased risk of adverse cardiovascular outcomes and death [Block, 1998 (5); London, 2003 (6)].
  • Calcium salts should be avoided in patients with sustained intact PTH levels of ⁇ 150 pg/mL, or plasma calcium levels of >9.5 mg/dL (>2.37 mmol/L). Vitamin D compounds should also be avoided or terminated in patients with calcium levels greater 9.5 mg/dL (>2.37 mmol/L).
  • Non-calcium-based phosphate binders are preferred in patients with severe vascular or soft-tissue calcifications.
  • Plasma calcium levels should be maintained at the lower end of the normal range (8.4 to 9.5 mg/dL [2.1 to 2.35 mmol/L]).
  • the calcium-phosphate product should be kept less than 55 mg2/mL2 ( ⁇ 4.4 mmol2/L2) by first focusing on controlling plasma phosphate.
  • cardiovascular complications which are the main cause of death in patients suffering from chronic kidney failure. At local level these complications are manifested by alterations of the endothelium, accumulation of lipids, formation of clots and occlusion of the lumen.
  • calcium based binders i.e. calcium acetate, calcium carbonate, calcium-magnesium-salts, aluminium based binders, i.e. aluminium chloride and aluminium hydrochloride,
  • iron containing phosphate binders iron citrate, sucroferric oxyhydroxide
  • phosphate lowering agents which act by physico-chemical absorption of phosphate taken in by diet and being absorbed by the polymer during the gastro-intestinal passage.
  • active and passive phosphate uptake can be reduced on a physiological mode of action:
  • phosphate lowering agents and "phosphate binders” are used herein interchangeably.
  • Nicotinamide acts in a pharmacological, pharmaco-physiological mode of action by down- regulating NaPi2b cotransporters predominantly expressed in the small intestine.
  • Extracellular phosphate homeostasis is achieved by the regulation of intestinal phosphate absorption as well as by regulation of phosphate excretion via the kidneys. Further, phosphate homeostasis is regulated by an integrated endogenous crosstalk involving kidney, bone and intestine (Ketteler, 201 1 (58)). Extracellular phosphate homeostasis is achieved by the regulation of intestinal phosphate absorption as well as by regulation of phosphate excretion via the kidneys.
  • NaPi2a, NaPi2c and NaPi2b sodium-dependent phosphate cotransporters
  • PiT1 and PiT2 type 3 cotransporters
  • NaPi2b cotransporters are essential for the active up-take of phosphate which contributes to ca. 50% of phosphate uptake into serum (Katai, 1999 (12)).
  • the kidneys express four different phosphate cotransporters.
  • NaPi2a NaPi2c, PiT2
  • CaPi2a NaPi2a, NaPi2c, PiT2
  • Their physiological role is the reabsorption of filtrated phosphate from the primary urine.
  • NaPi2b was also detected in the kidney of rats (Suyama, 2012 (1 1 )).
  • NaPi2b is expressed at the basolateral side of epithelial cells surrounding the urinary duct and it was suggested that the physiological role is to enhance basal phosphate excretion levels in the kidney.
  • BBM Brush Border Membrane
  • MEPE matrix extracellular phosphoglycoprotein
  • FGF23 Fibroblast growth factor 23
  • Mg 2+ Magnesium
  • NaPi Sodium phosphate cotransporter
  • PFA Phosphonoformic acid
  • PiT Sodium dependent phosphate cotransporter
  • P0 4 Phosphate
  • S Segment
  • VDR Vitamin D receptor
  • PTH Parathormone
  • nicotinamide can be effective in lowering elevated phosphate levels in animals with experimentally induced CKD (Eto, 2005 (18)) and in humans with end stage renal disease on dialysis (Takahashi, 2004 (19), Medice, 2015 (36)).
  • Sodium dependent phosphate cotransporter NaPi2b was shown to be responsible for around 50% of gastrointestinal phosphate absorption (Katai, 1999 (12)). Beneath this transcellular transport mechanism passive phosphate diffusion is also important in intestinal phosphate uptake.
  • intestinal NaPi2b is blocked by a phosphate-rich diet (Hattenhauer, 1999 (16)).
  • a low-phosphate diet (Giral, 2009 (13); Hattenhauer, 1999 (16)) or an increase in serum calcitriol (Xu, 2002 (17)) increases the expression of the cotransporter.
  • FGF23 was shown to exert an indirect inhibitory action on intestinal NaPi2b expression via inhibition of renal l ohydroxylase activity and therefore decreasing Calcitriol levels (Marks, 2010 (10)).
  • Intraperitoneal administration of nicotinamide blocks the expression of NaPi2b (Eto, 2005 (18)) and inhibits the gastrointestinal absorption of phosphate (Katai, 1999 (12)). It has not been established whether the functional cotransporter is also directly inhibited.
  • MR-NA modified release nicotinamide
  • phosphate homeostasis is regulated by an integrated endogenous crosstalk involving kidney, bone and intestine (Ketteler, 201 1 (58)). Decline of kidney function results in a cascade of pathophysiological events that result in mineral and bone disorder (MBD). MBD is characterized by progressive development of secondary hyperparathyroidism, arterial calcification, altered arterial function and abnormal bone metabolism. These changes contribute to further loss of kidney function, bone demineralization, fractures and high cardiovascular morbidity and mortality (KDIGO, 2009 (8)).
  • CKD-MBD CKD-MBD
  • PTH parathyroid hormone
  • FGF23 fibroblast growth factor 23
  • phosphate retention inhibits renal synthesis of 1 ,25 dihydroxyvitamin D (1 ,25 (OH)2D), resulting in reduced intestinal absorption of phosphate (Marks, 2006 (63)).
  • phosphaturic hormones and 1 ,25(OH)2D display characteristic changes in early kidney disease while blood phosphate levels remain in the normal range until CKD stage 3-4 followed by a strong exponential increase in advanced stages, especially in CKD stage 4/5.
  • CKD cardiovascular disease
  • CVD cardiovascular disease
  • Heart, 2004 (64) cardiovascular morbidity and mortality is strongly increased in CKD (Kestenbaum, 2005 (65)) and in patients with end stage renal disease (Block, 2004 (66)).
  • CVD cardiovascular disease
  • the 5 year survival rate of patients on hemodialysis is only 38% (Frei, 2008 (67)).
  • This extremely high mortality is driven by a 30- to 100-fold increase in age-, gender-, and race- adjusted cardiovascular mortality rates (Foley, 1998 (68)).
  • Altered mineral metabolism with raised blood phosphate levels hyperphosphatemia
  • CKD patients exhibit other risk factors for cardiovascular disease.
  • Dyslipidemia is a very common comorbidity of CKD patients.
  • CKD patients typically have high levels of triglycerides and especially patients with nephrotic syndrome exhibit a considerable increase of low-density lipoproteins (LDL) (Mikolasevic, 2017 (23)).
  • LDL lowering was demonstrated to reduce cardiovascular mortality in CKD patients (Baigent, 201 1 (24)) as well as in diabetic patients on hemodialysis (Marz, 201 1 (25) ).
  • Dyslipidemia results in the classical picture of atherosclerosis, defined by the formation of lipid deposits forming fatty streaks in the lumen of blood vessels, growing up to plaques of variable size that result in occlusion of vessels (Amann, 2008 (26) ).
  • LDL cholesterin lipoprotein(a)
  • LP(a) lipoprotein(a)
  • Apo(a) apolipoprotein(a)
  • Apo(a) competes with plasminogen for plasminogen receptors, fibrinogen, and fibrin.
  • high serum levels of Lp(a) are inversely correlated with all-cause death and acute coronary syndrome, indicating Lp(a) as an independent risk factor for cardiovascular events (Konishi, 2016 (70)).
  • patients with high levels of Lp(a) have a significantly raised risk for the development of CKD over a median follow-up period of 10 years (Yun, 2016 (71 )).
  • Lp(a) trigger both development and progression of CKD as well as elevated cardiovascular morbidity and mortality in patients with advanced CKD.
  • Lp(a) is a low density lipoprotein complexed with Apo(a). Apo(a) is produced almost exclusively in the liver and Lp(a) plasma levels highly correlate with Apo(a) production (Kostner, 2013 (72)). Up to date, pharmacological interventions to lower Lp(a) are very limited. Treatment with an PCSK9 inhibitor reduces Lp(a) by around 35% (Kotani, 2017 (29)). In addition nicotinic acid was shown to reduce Lp(a) also up to 35% (Carlson, 1989 (30)).
  • Nicotinic acid reduces Lp(a) plasma levels probably due to inhibition of hepatic Apo(a) gene expression (Chennamsetty, 2012 (31 )). In addition this pharmacological action is probably linked to binding of nicotinic acid to the G-protein-coupled receptor GPR109A (Digby, 2012 (32)). It is not known whether nicotinamide also has the potential to reduce Lp(a) plasma levels.
  • dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure, as well as preventing and/or treating elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, both particularly resulting from renal failure.
  • dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure.
  • the invention addresses the problem of dyslipidemia, as well as hyperphosphatemia and dyslipidemia, resulting from chronic kidney failure (CKD).
  • the invention provides a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for prophylaxis and/or treatment of dyslipidemia, as well as hyperphosphatemia and dyslipidemia, resulting particularly from chronic kidney failure (CKD) as well as for the treatment and prevention of End-Stage Renal Disease (ESRD).
  • the pharmaceutical preparation is administered preferably via the oral route or the parenteral route.
  • the invention further addresses the problem of limited efficacy of available treatment options in terms of dyslipidemia and dyslipidemia and reduction of blood phosphate levels in patients particularly with CKD 3-5, as dietary modifications of phosphate intake as well as treatment with phosphate binders are inefficient in the reduction of phosphate burden in moderate CKD (Sprague, 2009 (59), Oliveira, 2010 (60)).
  • the invention also provides a pharmaceutical preparation comprising a pharmaceutically effective amount of modified release nicotinamide for prophylaxis and/or treatment of dyslipidemia, as well as dyslipidemia and hyperphosphatemia, resulting particularly from CKD stages 3-5.
  • the inventors particularly also found in a further aspect an efficient reduction of elevated serum phosphate levels in patients with chronic kidney disease due to a dual mode of action.
  • the known pharmacological basis for the reduction of elevated serum phosphate levels is linked to the nicotinamide induced reduction of phosphate cotransporter NaPi2b in the intestine, resulting in reduced absorption of phosphate from food.
  • the invention shows that nicotinamide additionally reduces renal expression of cotransporter NaPi2b in individuals with residual renal function, resulting in enhanced excretion of phosphate via the kidneys.
  • This dual mode of action results in a stronger reduction of elevated phosphate levels compared to the treatment with conventional phosphate binders that act solely by binding of phosphate from ingested food in the intestine.
  • This invention involves the administration of a pharmaceutically effective quantity of nicotinamide.
  • the present invention relates to a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for use in a method of preventing and/or treating of dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure.
  • dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure.
  • Lp(a) serum Lipoprotein(a)
  • a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for use in a method of preventing and/or treating of elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, both particularly resulting from renal failure.
  • elevated serum phosphate levels hyperphosphatemia
  • dyslipidemia particularly dysregulation of lipid metabolism
  • elevation of serum Lipoprotein(a) (Lp(a)) levels both particularly resulting from renal failure.
  • Fig. 1 refers to results obtained in present Example 1 and shows the reduction of Lp(a) plasma levels in transgenic Apo(a) mice treated either with 1 % nicotinic acid (A) or nicotinamide (B). After 1 week of treatment only nicotinamide reduced Lp(a) levels significantly. After 2 weeks of treatment Lp(a) plasma levels were more than 50% lower compared to nicotinic acid and more than 200% lower compared to baseline.
  • Fig. 2a illustrates quantification of NaPi2b protein expression in a mouse model of chronic kidney disease.
  • WT wild type mice
  • adenine induced CKD resulted in small reductions of the NaPi2b phosphate cotransporter while treatment with the phosphate binder sevelamer resulted in a strong upregulation of NaPi2b protein expression.
  • Fig. 2b represents serum phosphate levels in two different strains of mice with experimentally induced CKD.
  • WT wild type mice
  • adenine induced CKD resulted in a significant rise of phosphate levels.
  • Treatment with the phosphate binder sevelamer did not lower elevated serum phosphate.
  • NaPi-KO NaPi2b-Knock out mice
  • Fig. 3 shows results obtained in present Example 2.
  • treatment with the phosphate binder magnesium carbonate (Mg) resulted in a strong enhancement of NaPi2b protein expression. This upregulation was completely abolished under combined treatment with nicotinamide (NA) and phosphate binder.
  • NA nicotinamide
  • Fig. 4 depicts further results obtained in Example 2.
  • treatment with nicotinamide resulted in a strong increase of NaPi2b protein expression in the kidneys.
  • Combined treatment of nicotinamide and the phosphate binder magnesium carbonate further enhanced renal NaPi2b.
  • Fig. 5 shows a schematic of the supposed mode of action of nicotinic acid in reduction of Lp(a). Nicotinic acid binds specifically to the nicotinic acid receptor GRP109A (Tunaru 2005 (33)). After ligand binding the G-protein-coupled receptor inhibits intracelluar adenylatcylases that catalyze cyclic adenosine monophosphate generation (cAMP) from adenosine triphosphate (ATP). The translation of the apoprotein A gene is inhibited as the promotor region of the gene contains c- AMP response elements (cAMP-RE) (Gouni-Berthold, 2013 (34)).
  • Fig. 6 shows differences of serum Lp(a) concentration compared to screening in the ITT population in Example 4.
  • Fig. 7 depicts the course of serum phosphate concentrations during the trial of Example 5 in patients that completed the study.
  • Fig. 8 shows the phosphate levels (mmol/l) in CKD patients on hemodialysis in response to conventional phosphate binders or tenapanor in combination with nicotinamide (NA) (dosing in mg, oral, once daily) over time (weeks).
  • NA nicotinamide
  • the phosphate binders of the invention are also named phosphate lowering agents and are known in the art per se. According to the invention, also other phosphate binders acting in lowering the phosphate level can be used within the scope of the invention.
  • phosphate lowering agents and “phosphate binders” are used herein, within the scope of the invention, interchangeably.
  • a pharmaceutical preparation comprising modified release nicotinamide is a pharmaceutical preparation comprising nicotinamide in which the whole dose of the nicotinamide contained in the pharmaceutical preparation is not released directly upon taking of the pharmaceutical preparation, but is released upon and/or over a certain time, i.e. is in an extended release preparation/ a sustained release preparation. It shows a slower release of the nicotinamide than a conventional-release dosage form administered by the same route, i.e. an immediate release preparation.
  • a pharmaceutically effective amount of nicotinamide e.g. also modified release nicotinamide, can be an amount of nicotinamide in the pharmaceutical preparation that can achieve a therapeutic response or desired effect in some fraction of the subjects taking the pharmaceutical preparation.
  • elevated phosphate levels are phosphate levels which exceed those recommended by medical guidelines, e.g. serum phosphate levels exceeding about 5.5 mg/dl and/or with serum phosphate levels about ⁇ 1.78mmol/l.
  • dyslipidemia is represented by an abnormal amount of lipids (e.g. triglycerides, cholesterol, fat phospholipids) or substances derived thereof, e.g. lipoproteins, in the patient, particularly in the blood. According to certain embodiments, it refers to a dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels.
  • lipids e.g. triglycerides, cholesterol, fat phospholipids
  • lipoproteins e.g. lipoproteins
  • a first aspect of the present invention relates to a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for use in a method of preventing and/or treating of dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure.
  • dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure.
  • Lp(a) serum Lipoprotein(a)
  • a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for use in a method of preventing and/or treating of elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, both particularly resulting from renal failure.
  • elevated serum phosphate levels hyperphosphatemia
  • dyslipidemia particularly dysregulation of lipid metabolism
  • Lp(a) serum Lipoprotein(a)
  • the present invention is directed to a method of preventing and/or treating elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, particularly resulting from renal failure, using a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide.
  • the present invention relates to a method of preventing and/or treating dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, both particularly resulting from renal failure, using a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide.
  • said dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, or said hyperphosphatemia and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, result from chronic kidney failure, from of end- stage renal disease, and/or from hemodialysis.
  • the pharmaceutical preparations of the first and/or second aspect and/or in the third and/or fourth aspect are administered parenterally or orally, preferably orally.
  • the pharmaceutical preparations comprising a pharmaceutically effective amount of nicotinamide for use in a method of the first or second aspect, as well as the pharmaceutical preparation used in the third or fourth aspect, can comprise further constituents which are not particularly restricted, like e.g. at least one pharmaceutically acceptable carrier and/or excipients like anti-adherents; binders, like saccharides and their derivatives, e.g.
  • disaccharides like sucrose, lactose; polysaccharides and their derivatives like starches, cellulose or modified cellulose like microcrystalline cellulose and cellulose ethers such as hydroxypropyl cellulose; sugar alcohols like xylitol, sorbitol and maltitol; proteins like gelatin; or synthetic polymers like polyvinyl pyrrolidone or polyethylene glycol, etc., e.g. microcrystalline cellulose; softening agents like dibutyl sebacate, tributyl citrate, triethyl citrate, acetyl triethyl citrate, etc., e.g.
  • dibutyl sebacate, and/or separating agents and/or flow aids like glycerolmonostearate, talc and/or colloidal anhydrous silica; coatings; network forming excipients; colours; disintegrants; flavors; fillers; diluents; glidants like fumed silica, talc, magnesium stearate and/or magnesium carbonate; lubricants like talc, silica and/or fats; preservatives like antioxidants, e.g. vitamin A, vitamin E, vitamin C, etc., the amino acids cysteine and methionine, citric acids and salts thereof, e.g.
  • sodium citrate and/or synthetic preservatives; sorbents, like desiccants; sweeteners; water stabilizers; antifungals and/or vehicles which preferably do not interact with the nicotinamide and/or at least one phosphate binder.
  • the pharmaceutical preparations of the first and/or second aspect and/or used in the third and/or fourth aspect are in the form of tablets, capsules, oral preparations, powders, granules, lozenges, reconstitutable powders, syrups, solutions or suspensions.
  • the pharmaceutical preparation comprises a formulation comprising nicotinamide which can be in the form of pellets, i.e. comprises one or more pellets, e.g. a multitude of pellets.
  • the pharmaceutical preparations are in the form of a capsule comprising pellets of nicotinamide.
  • the material of the capsule is not particularly restricted. According to certain embodiments the material of the capsule does not lead to an extended release of the pellets of nicotinamide and preferably dissolved immediately at a pH of about 3 or less, e.g. about 2 or less or about 1.5 or less. According to certain embodiments, the capsule dissolves independently of the pH.
  • the capsule can be a hard capsule or a soft capsule, e.g. a hard capsule, e.g. formed of a capsule cap and a capsule body, both of which are not particularly restricted and which can e.g. contain pharmaceutically acceptable excipients as listed above regarding the pharmaceutical preparation.
  • the capsule cap can contain materials like gelatin; colors, e.g. titanium dioxide, indigo carmine, black iron oxide, and/or erythrosine; sodium lauryl sulfate; and/or purified water
  • the capsule body can contain materials like gelatin; titanium dioxide; sodium lauryl sulfate; and/or purified water.
  • the subject is a mammal, particularly a human.
  • the nicotinamide is to be administered in unit doses up to about 2000 mg per day, preferably in unit doses ranging from about 100 to about 2000 mg per day, e.g. from about 200 or about 250 to about 2000 mg per day, further preferably from about 400 to about 1700 mg per day, even further preferably from about 500 to about 1500 mg per day.
  • the unit doses can be e.g. administered in 2 to 3 separate doses according to certain embodiments.
  • the nicotinamide is to be administered before, with and/or after meals, e.g. within 1 hour or within 30 minutes after meals, and/or before going to bed, e.g. within 1 hour or within 30 minutes before going to bed, independently from food intake and before and/or after hemodialysis or peritoneal dialysis treatment.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is administered once or twice daily independently from food intake, preferably once daily, further preferably before going to bed. Particularly with an administration once before going to bed a simultaneous taking of a phosphate binder can be avoided which might otherwise negatively affect the taking of the nicotinamide as an add-on.
  • further at least one phosphate binder is administered.
  • the phosphate binder is not particularly restricted in this regard and those usually applied for the treatment of hyperphosphatemia and/or dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, can be applied.
  • the phosphate binder is at least one selected from the group comprising
  • calcium based binders e.g. calcium acetate, calcium carbonate, calcium-magnesium-salts, aluminium based binders, e.g. aluminium chloride and aluminium-hydrochloride, aluminium chloride hydroxide complex,
  • iron containing phosphate binders e.g. iron citrate, sucroferric oxyhydroxide, and / or sevelamer, sevelamer carbonate or sevelamer HCI (polymers),
  • the at least one phosphate binder is at least one selected from the group comprising calcium acetate, calcium-magnesium-salts, tenapanor, sevelamer, sevelamer carbonate, calcium carbonate, magnesium carbonate, lanthanum carbonate, aluminium chloride hydroxide complex, and mixtures thereof. Also complexes and/or adducts of these phosphate binders are possible, e.g. with water.
  • the phosphate binder comprises or is magnesium carbonate.
  • the phosphate binder comprises or is calcium- magnesium salts.
  • the phosphate binder comprises or is tenapanor.
  • the phosphate binder is selected from the phosphate binders given in Table 7 or in Table 8.
  • Usual unit doses may vary according to phosphate binder applied, while, at least for some patients, the recommended daily dose (KDIGO 2009, DIMDI and WHO ATC defined daily doses) can be as follows,
  • calcium based binders e.g. calcium acetate (about 5600 - 6300 mg/d, e.g. ca. 6000 mg/d), calcium carbonate (ca. 4000 mg/d), calcium-magnesium-salts (about 4000 - 4500 mg/d, e.g. ca- 4226 mg/d), not exceeding the recommended daily unit dose of ca. 1500 mg elementary calcium per day
  • aluminium-based binders e.g. aluminium chloride, AI 9 Cl 8 (OH) 19 (about 900 - 1800 mg/d) and aluminium hydrochloride (about 1800 - 12000 mg/d)
  • daily dose is e.g. ca. 1800 mg/d
  • daily dose is e.g. about 3708 mg/d, and/or average daily dose is e.g. about 2250 mg/d
  • iron containing phosphate binders e.g. iron citrate, sucroferric oxyhydroxide
  • daily dose is ca. 7200 - 7500 mg/d
  • sevelamer carbonate or sevelamer HCI polymers
  • daily dose is ca. 5600 - 6400 mg/d
  • tenapanor daily dose is ca. 1 - 100mg/d.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is administered at a time different from the administration of the at least one phosphate binder, particularly if the at least one phosphate binder negatively affects the nicotinamide uptake, preferably with a time difference of at least one hour, further preferably at least two hours, even further preferably at least three hours. It was found that phosphate binders like sevelamer can negatively affect the intestinal absorption of nicotinamide, presumably by complexing it.
  • the phosphate binder and the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide are given at different times, particularly if the at least one phosphate binder negatively affects the nicotinamide uptake.
  • the time difference to the next taking of phosphate binder after the taking of the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is at least one hour, preferably at least two hours, particularly preferably at least three hours, so that the nicotinamide can be released without an interference of phosphate binder.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is taken before sleeping so that the time difference to the next taking of phosphate binder, which is usually taken together with a meal, is maximized, e.g. also when nicotinamide is not immediately released from the pharmaceutical preparation, i.e using a modified release pharmaceutical preparation.
  • a pharmaceutically effective amount of nicotinamide with sufficient difference to the time of taking phosphate binder is suitable.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is taken together with the phosphate binder if the phosphate binder does essentially not negatively affect the nicotinamide uptake.
  • the at least one phosphate binder is not sevelamer and/or a derivative thereof, e.g. sevelamer hydrochloride and/or sevelamer carbonate, particularly when administered concomitantly with the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide.
  • the at least one phosphate binder is calcium acetate, calcium carbonate and/or lanthanum carbonate and/or an aluminium containing phosphate binder and/or a phosphate binder containing iron, as e.g. given above.
  • a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide, e.g. modified release nicotinamide, for use in a method of preventing and/or treating of dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, or elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, in patients in phases 4 and/or 5 of chronic kidney disease, excluding patients undergoing dialysis treatment, both particularly resulting from renal failure.
  • nicotinamide e.g. modified release nicotinamide
  • the patients have a glomerular filtration rate of 30 ml/min/1 .73 m 2 or less and 10 ml/min/1 .73 m 2 or more, preferably less than 30 ml/min/1 .73 m 2 and more than 10 ml/min/1 .73 m 2 , and/or do not undergo dialysis treatment.
  • a method of preventing and/or treating dyslipidemia particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, or elevated serum phosphate levels (hyperphosphatemia) and dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, both particularly resulting from renal failure, using a pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide, e.g. modified release nicotinamide, e.g. as defined above with regard to the second aspect.
  • nicotinamide e.g. modified release nicotinamide
  • the methods is applied to patients having a glomerular filtration rate of 30 ml/min/1 .73 m 2 or less and 10 ml/min/1 .73 m 2 or more, preferably less than 30 ml/min/1 .73 m 2 and more than 10 ml/min/1 .73 m 2 , and/or do not undergo dialysis treatment.
  • the pharmaceutical preparation of the fifth aspect and/or in the sixth aspect is administered parenterally or orally, preferably orally.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide for use in a method of the fifth aspect, as well as the pharmaceutical preparation used in the sixth aspect, can comprise further constituents which are not particularly restricted, like e.g. at least one pharmaceutically acceptable carrier and/or excipients like antiadherents; binders, like saccharides and their derivatives, e.g.
  • disaccharides like sucrose, lactose; polysaccharides and their derivatives like starches, cellulose or modified cellulose like microcrystalline cellulose and cellulose ethers such as hydroxypropyl cellulose; sugar alcohols like xylitol, sorbitol and maltitol; proteins like gelatin; or synthetic polymers like polyvinyl pyrrolidone or polyethylene glycol, etc., e.g. microcrystalline cellulose; softening agents like dibutyl sebacate, tributyl citrate, triethyl citrate, acetyl triethyl citrate, etc. , e.g.
  • dibutyl sebacate, and/or separating agents and/or flow aids like glycerolmonostearate, talc and/or colloidal anhydrous silica; coatings; network forming excipients; colours; disintegrants; flavors; fillers; diluents; glidants like fumed silica, talc, magnesium stearate and/or magnesium carbonate; lubricants like talc, silica and/or fats; preservatives like antioxidants, e.g. vitamin A, vitamin E, vitamin C, etc., the amino acids cysteine and methionine, citric acids and salts thereof, e.g.
  • sodium citrate and/or synthetic preservatives; sorbents, like desiccants; sweeteners; water stabilizers; antifungals and/or vehicles which preferably do not interact with the nicotinamide and/or at least one phosphate binder.
  • the pharmaceutical preparation of the fifth aspect and/or used in the sixth aspect is in the form of tablets, capsules, oral preparations, powders, granules, lozenges, reconstitutable powders, syrups, solutions or suspensions.
  • the pharmaceutical preparation comprises a formulation comprising nicotinamide which can be in the form of pellets, i.e. comprises one or more pellets, e.g. a multitude of pellets.
  • the pharmaceutical preparation is in the form of a capsule comprising pellets of nicotinamide.
  • the material of the capsule is not particularly restricted. According to certain embodiments the material of the capsule does not lead to an extended release of the pellets of nicotinamide and preferably dissolved immediately at a pH of about 3 or less, e.g. about 2 or less or about 1 .5 or less. According to certain embodiments, the capsule dissolves independently of the pH.
  • the capsule can be a hard capsule or a soft capsule, e.g. a hard capsule, e.g. formed of a capsule cap and a capsule body, both of which are not particularly restricted and which can e.g. contain pharmaceutically acceptable excipients as listed above regarding the pharmaceutical preparation.
  • the capsule cap can contain materials like gelatin; colors, e.g. titanium dioxide, indigo carmine, black iron oxide, and/or erythrosine; sodium lauryl sulfate; and/or purified water
  • the capsule body can contain materials like gelatin; titanium dioxide; sodium lauryl sulfate; and/or purified water.
  • the subject is a mammal, particularly a human.
  • the nicotinamide is to be administered in unit doses up to about 2000 mg per day, preferably in unit doses ranging from about 250 to about 2000 mg per day, further preferably from about 400 to about 1700 mg per day, even further preferably from about 500 to about 1500 mg per day.
  • the nicotinamide is to be administered before, with and/or after meals, e.g. within 1 hour or within 30 minutes after meals, and/or before going to bed, e.g. within 1 hour or within 30 minutes before going to bed, independently from food intake and before and/or after hemodialysis or peritoneal dialysis treatment.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is administered once or twice daily independently from food intake, preferably once daily, further preferably before going to bed. Particularly with an administration once before going to bed a simultaneous taking of a phosphate binder can be avoided which might otherwise negatively affect the taking of the nicotinamide as an add-on.
  • further at least one phosphate binder is administered.
  • the phosphate binder is not particularly restricted in this regard and those usually applied for the treatment of hyperphosphatemia and/or dyslipidemia, particularly dysregulation of lipid metabolism, particularly elevation of serum Lipoprotein(a) (Lp(a)) levels, can be applied.
  • the phosphate binder is at least one selected from the group comprising
  • calcium based binders e.g. calcium acetate, calcium carbonate, calcium- magnesium-salts,
  • aluminium based binders e.g. aluminium chloride and aluminium-hydrochloride, aluminium chloride hydroxide complex
  • iron containing phosphate binders e.g. iron citrate, sucroferric oxyhydroxide, and / or
  • the at least one phosphate binder is at least one selected from the group comprising calcium acetate, calcium-magnesium-salts, sevelamer, sevelamer carbonate, calcium carbonate, magnesium carbonate, lanthanum carbonate, aluminium chloride hydroxide complex, and mixtures thereof. Also complexes and/or adducts of these phosphate binders are possible, e.g. with water.
  • the phosphate binder comprises or is magnesium carbonate.
  • the phosphate binder comprises or is calcium-magnesium salts.
  • the phosphate binder comprises or is tenapanor.
  • the phosphate binder is selected from the phosphate binders given in Table 7 or in Table 8.
  • Usual unit doses may vary according to phosphate binder applied, while, at least for some patients, the recommended daily dose (KDI GO 2009, DI MDI and WHO ATC defined daily doses) can be as follows,
  • calcium based binders e.g. calcium acetate (about 5600 - 6300 mg/d, e.g. ca. 6000 mg/d), calcium carbonate (ca. 4000 mg/d), calcium-magnesium-salts (about 4000 - 4500 mg/d, e.g. ca- 4226 mg/d), not exceeding the recommended daily unit dose of ca. 1500 mg elementary calcium per day
  • aluminium-based binders e.g. aluminium chloride, AI 9 Cl 8 (OH) 19 (about 900 - 1800 mg/d) and aluminium hydrochloride (about 1800 - 12000 mg/d)
  • daily dose is e.g. ca. 1800 mg/d
  • daily dose is e.g. about 3708 mg/d, and/or average daily dose is e.g. about 2250 mg/d
  • iron containing phosphate binders e.g. iron citrate, sucroferric oxyhydroxide
  • daily dose is ca. 7200 - 7500 mg/d
  • sevelamer carbonate or sevelamer HCI polymers
  • daily dose is ca. 5600 - 6400 mg/d
  • tenapanor, a sodium hydrogen exchange blocking agent, daily dose is ca. 1 - 100mg/d.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is administered at a time different from the administration of the at least one phosphate binder, particularly if the at least one phosphate binder negatively affects the nicotinamide uptake, preferably with a time difference of at least one hour, further preferably at least two hours, even further preferably at least three hours. It was found that phosphate binders like sevelamer can negatively affect the intestinal absorption of nicotinamide, presumably by complexing it.
  • the phosphate binder and the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide are given at different times, particularly if the at least one phosphate binder negatively affects the nicotinamide uptake.
  • the time difference to the next taking of phosphate binder after the taking of the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is at least one hour, preferably at least two hours, particularly preferably at least three hours, so that the nicotinamide can be released without an interference of phosphate binder.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is taken before sleeping so that the time difference to the next taking of phosphate binder, which is usually taken together with a meal, is maximized, e.g. also when nicotinamide is not immediately released from the pharmaceutical preparation.
  • the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide is taken together with the phosphate binder if the phosphate binder does essentially not negatively affect the nicotinamide uptake.
  • the at least one phosphate binder is not sevelamer and/or a derivative thereof, e.g. sevelamer hydrochloride and/or sevelamer carbonate, particularly when administered concomitantly with the pharmaceutical preparation comprising a pharmaceutically effective amount of nicotinamide.
  • the at least one phosphate binder is calcium acetate, calcium carbonate and/or lanthanum carbonate and/or an aluminium containing phosphate binder and/or a phosphate binder containing iron, as e.g. given above.
  • Example 1 The present invention will now be described in detail with reference to several examples thereof. However, these examples are illustrative and do not limit the scope of the invention.
  • Example 1 Example 1 :
  • Nicotinamide for the improvement of Lp(a) levels in patients with CKD Nicotinamide for the improvement of Lp(a) levels in patients with CKD.
  • Lp(a) is biosynthesized only in humans and old world monkeys which complicates the use of animal models to investigate Lp(a) metabolism directly (Kostner, 2013 (72)).
  • Lp(a) consists of LDL covalently bound to Apo(a).
  • Plasma levels of Lp(a) highly correlate with Apo(a) synthesis in man.
  • Lp(a) synthesis is limited to primates, a transgenic mouse model was used where the entire Apo(a) gene including the promotor region was introduced to the mouse genome (Chennamsetty, 2012 (31 )).
  • Apo(a) is expressed mainly in female mice. These female mice were shown to exhibit a 43% reduction in plasma Apo(a) protein as well as a 65% reduction in Apo(a) mRNA transcript in liver cells in response to oral treatment with 1 % nicotinic acid in the chow (Chennamsetty, 2012 (31 )).
  • Nicotinic acid binds to the specific nicotinic acid receptor GPR109A.
  • GPR109A inhibits adenylatcyclase responsible for the synthesis of adenosine- triphosphate (ATP) to cyclic adenosine-monophosphate (cAMP).
  • ATP adenosine- triphosphate
  • cAMP-RE nuclear cAMP response elements
  • heterozygote tg-Apo(a) female mice received either 1 % nicotinamide or 1 % nicotinic acid orally as food supplements in a crossover design for two weeks.
  • Levels of Apo(a) were determined by means of ELISA and were expressed as Lp(a) in mg/dl as each molecule Apo(a) binds one molecule of LDL to form Lp(a). Nicotinamide treatment resulted in a 67% reduction of Lp(a) after week 1 and a 77% reduction after two weeks of treatment compared to baseline levels (p ⁇ 0.0001 ; t-Test and Wilcox), as also shown in Fig. 1.
  • Figure 1 shows the reduction of serum levels Lp(a) in transgenic Apo(a) female mice treated with standard chow (Maintenance Diet, Altromin Spezialfutter GmbH & Co. KG, Germany: crude protein 191970.400 [mg/kg]; crude fat 40803.010 [mg/kg]; crude fiber 60518.480 [mg/kg]; crude ash 69364.890 [mg/kg]; moisture 1 12946.890 [mg/kg]; disaccharide(s) 49464.050 [mg/kg]; polysaccharides 358852.330 [mg/kg]; metab.
  • Lp(a) As discussed above, high levels of Lp(a) trigger both the development and progression of CKD as well as elevated cardiovascular morbidity and mortality in patients with advanced CKD. As dyslipidemia and hyperphosphatemia on one hand both synergistically trigger development of cardiovascular calcifications (see above) and on the other hand progression of CKD is an independent risk factor for incidence and severity of hyperphosphatemia, lowering of elevated levels of Lp(a) might be beneficial in CKD patients both in terms of reducing the risk and frequency of hyperphosphatemic periods as well as lowering the risk for cardiovascular outcomes related to hyperphosphatemia.
  • nicotinamide reduces risk and strength of hyperphosphatemia in patients with CKD by dual action on (Kettler, 201 1 (58)) the inhibition of the intestinal NaPi-2b cotransporter as well as by lowering serum levels of Lp(a). Moreover, as Lp(a) and hyperphosphatemia both promote cardiovascular calcification, both action may be beneficial in improvement of clinical outcomes.
  • a combination therapy approach using nicotinamide and a phosphate binder is especially promising in the treatment of particularly hyperphosphatemia because the pharmacological target of nicotinamide, the cotransporter NaPi2b is strongly regulated up under treatment with phosphate binders.
  • a phosphate binder that negatively affects the nicotinamide uptake e.g. like sevelamer and/or a derivative thereof, can alleviate these negative effects of such phosphate binder when given concomitantly.
  • Pi was significantly elevated in uremic (Adenine) WT mice. This effect was attenuated in uremic NaPi-KO mice. Phosphate binder treatment (Adenine + Sevelamer) normalized Pi in NaPi-KO mice while elevated Pi levels remained unaffected in WT mice, indicating that phosphate binder treatment was counteracted by compensatory regulation of the NaPi2b cotransporter.
  • adenine treatment reduced intestinal NaPi2b protein expression by 50% while these animals experienced a 6-fold increase of the cotransporter under phosphate binder treatment (Fig. 2a), while the cotransporter protein was not detectable in NaPi-KO mice.
  • Adenine treatment generally resulted in hyperphosphatemia although phosphate levels in WT mice were significantly higher than in NaPi-KO mice (Fig. 2b).
  • phosphate binder treatment did not affect hyperphosphatemia while NaPi-KO mice were normophosphatemic under binder treatment (Fig 2b) indicating that in WT mice the upregulation of NaPi2b completely counteracted the lower phosphate availability due to binder treatment. Phosphate binder triggered upregulation of NaPi2b is completely abolished under combination therapy with nicotinamide.
  • mice 5/6 nephrectomized DBA/2 mice were used as model for vascular calcification.
  • the mice were treated with nicotinamide (NA) in a concentration of 600 ⁇ g/mL in the drinking water.
  • NA nicotinamide
  • Figure 3 shows NaPi2b immune fluorescence in a mouse model of chronic kidney disease (CKD).
  • CKD Mg intestinal NaPi2b protein density
  • CKD NA Mg nicotinamide and phosphate binder
  • nicotinamide treatment for lowering of phosphate burden is especially effective in combination therapy with therapeutic approaches intended for the restriction of intestinal phosphate availability, namely dietary phosphate restriction as well as oral phosphate binder treatment by
  • the kidneys express three different phosphate cotransporters (NaPi2a, NaPi2c, PiT2). They are located in the proximal part of the tubule apparatus, at the apical side of kidney epithelial cells (Forster, 2013 (56)). Their physiological role is the reabsorption of filtrated phosphate from the primary urine. Recently, the phosphate cotransporter NaPi2b was detected in the kidney of rats (Suyama, 2012 (1 1 )). In contrast to the cotransporters mentioned above, NaPi2b is expressed at the basolateral side of epithelial cells surrounding the urinary duct and it was suggested that the physiological role is to enhance basal phosphate excretion levels in the kidney.
  • renal NaPi2b expression is strongly enhanced under high phosphorus diet (Suyama, 2012 (1 1 )).
  • renal expression of NaPi2b was also significantly enhanced, while expression of NaPi2a and NaPi2c was reduced (Pulskens, 2015 (57)).
  • the present inventors explored the effect of nicotinamide treatment on renal NaPi2b cotransporter expression in 5/6 nephrectomized DBA/2 mice.
  • CKD induced an enhancement of renal NaPi2b RNA as well as renal protein expression.
  • nicotinamide treatment resulted in a strong and significant enhancement of renal NaPi2b expression, as shown in Figure 4.
  • FIG. 4 shows therein the quantification of renal expression of NaPi2b protein. Shown are the amount and standard deviation of NaPi2b protein immune fluorescence in control DBA/2 mice treated with nicotinamide (ctrl + NA), 5/6 nephrectomized mice (CKD), nicotinamide treated CKD mice (CKD + NA), mice treated with the phosphate binder magnesium carbonate (CKD + Mg) and animals treated both with nicotinamide and phosphate binder (CKD + NA + Mg). CKD resulted in slightly enhanced expression of NaPi2b in the remaining kidney tissue. Treatment of CKD mice with nicotinamide strongly increased NaPi2b protein signal.
  • NaPi2b cotransporter was additionally increased under combination treatment with the phosphate binder while treatment with the phosphate binder alone showed no significant difference compared to baseline. Bars between columns indicate significant between groups differences (ANOVA with Tukey Post Test, p ⁇ 0.05).
  • phosphate binders do not specifically react with phosphate but also bind other polar small molecules in the gut and thereby may inhibit their intestinal absorption (see e.g. Neradova, 2016 (55)).
  • nicotinamide is ineffective in the treatment of hyperphosphatemia when given in combination with the phosphate binder sevelamer (see Olivero, 2006 (43)), which is known for its broad intestinal interaction potential.
  • the summary of product characteristics for sevelamer carbonate (Sevemed®) states the following: "Sevemed is not absorbed and may affect the bioavailability of other medicinal products.
  • the medicinal product When administering any medicinal product where a reduction in the bioavailability could have a clinically significant effect on safety or efficacy, the medicinal product should be administered at least one hour before or three hours after Sevemed, or the physician should consider monitoring blood levels.” Due to their mode of action phosphate binders must be taken three times daily with meals and thus consequently can interact with immediate release nicotinamide, which usually must be taken also twice or three times daily.
  • Example 1 it was demonstrated on basis of an animal model of dysplipidemia that oral treatment with nicotinamide resulted in a significant reduction of Lipoprotein a (Lp(a)).
  • MR modified release
  • N number of patients
  • PB Individual Phosphate Binder
  • SEM Standard Error of Means
  • W Treatment Week
  • ITT Intention to Treat Population
  • Lp(a) Lipoprotein a
  • N number of patients
  • PB Individual Phosphate Binder
  • SEM Standard Error of Means
  • W treatment week
  • Figure 6 shows the difference of serum Lp(a) concentration compared to screening in the ITT population of the trial.
  • Lp(a) Lipoprotein a
  • PB individual Phosphate Binder
  • W treatment Week
  • the phosphate cotransporter NaPi2b was shown to be responsible for around 50% of gastrointestinal phosphate absorption (Katai et al. 1999 (12)). It has been shown in animal models, that nicotinamide reduces the intestinal expression of NaPi2b and the intestinal absorption of phosphate (Eto et al. 2005 (18)). In humans with end stage renal disease on dialysis nicotinamide reduced elevated serum phosphate concentrations significantly (Takahashi et al. 2004, (19), further data not shown). Standard approaches for the treatment of enhanced serum phosphate levels like low-phosphate diets increase the expression of the cotransporter NaPi2b (Giral et al. 2009 (13), Hattenhauer et al. 1999 (16)).
  • the treatment with phosphate binders was shown to enhance the intestinal expression of NaPi2b (Schiavi et al. 2012 (39)).
  • the adaptive upregulation of intestinal phosphate absorption via NaPi2b reduces the efficacy of routine approaches to treat hyperphosphatemia and might be responsible for the poor target achievement with these treatment approaches.
  • the inventors undertook a clinical trial to investigate the combined therapy of phosphate binders together with modified release (MR) nicotinamide to reduce serum phosphate levels in patients that were hyperphosphatemic under treatment with one or two phosphate binders.
  • MR modified release
  • Table 7 lists the 10 most commonly used phosphate binders in course of the trial.
  • patients under combination therapy with two different phosphate binders could participate in the trial.
  • Table 8 lists the number of patients for the 10 most common phosphate binder combination therapies.
  • Subgroup analysis for 3 groups of phosphate binders also revealed significant reductions in serum phosphate for the combination therapy of MR nicotinamide with 1 ) calcium containing phosphate binders, 2) lanthanum carbonate and aluminium salts and 3) the remaining phosphate binders (predominantly sevelamer carbonate and sevelamer hydrochloride) and 4) tenapanor, a sodium hydrogen exchange blocking agents, which blocks the passive uptake of phosphate in the intestine and in combination with nicotinamide, which blocks the active uptake of phosphate by blocking NaPi2b receptors located in the intestine which translates into a synergistic mode of action and in consequence, very effective lowering of phosphate serum levels.
  • This enhanced effect using a combination of nicotinamide and tenapanor, and to a lesser degree using a combination of nicotinamide with calcium-magnesium-salts (MagnesiumCalcium) is shown in Fig
  • Table 7 The 10 most common phosphate binders that were combined with either MR nicotinamid or Placebo in course of the trial
  • Table 8 The 10 most common combinations of two phosphate binder that were combined with either MR nicotinamid or Placebo in course of the trial
  • Circulating FGF-23 is regulated by 1 alpha,25-dihydroxyvitamin D3 and phosphorus in vivo. J Biol Chem. 2005;280(4):2543-9.
  • Block GA Hulbert-Shearon TE, Levin NW, Port FK. Association of serum phosphorus and calcium x phosphate product with mortality risk in chronic hemodialysis patients: a national study. Am J Kidney Dis. 1998;31 (4):607-17.
  • K/DOQI Clinical Practice Guidelines for Bone Metabolism and Disease in Chronic Kidney Disease (K/DOQI clinical practise guideline). American Journal of Kidney Diseases. 2003;42(4):s7-s201.
  • KDIGO Clinical Practice Guideline for the Diagnosis, Evaluation, Prevention, and Treatment of Chronic Kidney Disease-Mineral and Bone Disorder (CKD-MBD). Kidney Int 2009;76(Supplement 1 13):S1-S130.
  • Nicotinic acid inhibits hepatic APOA gene expression: studies in humans and in transgenic mice. J Lipid Res. 2012;53(1 1 ):2405-12.

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Abstract

L'invention concerne une préparation pharmaceutique comprenant du nicotinamide destinée à être utilisée dans un procédé de prévention et/ou de traitement de la dyslipidémie, résultant en particulier d'une insuffisance rénale et une préparation pharmaceutique comprenant du nicotinamide destinée à être utilisée dans un procédé de prévention et/ou de traitement de taux élevés de phosphate sérique (hyperphosphatémie) et de la dyslipidémie, tous deux résultant en particulier d'une insuffisance rénale.
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CN110869019A (zh) 2020-03-06
BR112019022889A2 (pt) 2020-05-19
JP2020518615A (ja) 2020-06-25
MX2019012970A (es) 2019-12-16
WO2018202704A1 (fr) 2018-11-08
RU2741426C1 (ru) 2021-01-26

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