EP3481945A4 - Rekombinante polypeptide mit selenocystein und verfahren zur herstellung davon - Google Patents

Rekombinante polypeptide mit selenocystein und verfahren zur herstellung davon Download PDF

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Publication number
EP3481945A4
EP3481945A4 EP17828247.1A EP17828247A EP3481945A4 EP 3481945 A4 EP3481945 A4 EP 3481945A4 EP 17828247 A EP17828247 A EP 17828247A EP 3481945 A4 EP3481945 A4 EP 3481945A4
Authority
EP
European Patent Office
Prior art keywords
selenocysteine
producing
same
recombinant polypeptides
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17828247.1A
Other languages
English (en)
French (fr)
Other versions
EP3481945A1 (de
Inventor
Andrew D. Ellington
Ross Thyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Publication of EP3481945A1 publication Critical patent/EP3481945A1/de
Publication of EP3481945A4 publication Critical patent/EP3481945A4/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Oncology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
EP17828247.1A 2016-07-11 2017-07-10 Rekombinante polypeptide mit selenocystein und verfahren zur herstellung davon Pending EP3481945A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662360745P 2016-07-11 2016-07-11
PCT/US2017/041373 WO2018013481A1 (en) 2016-07-11 2017-07-10 Recombinant polypeptides comprising selenocysteine and method for producing the same

Publications (2)

Publication Number Publication Date
EP3481945A1 EP3481945A1 (de) 2019-05-15
EP3481945A4 true EP3481945A4 (de) 2020-07-29

Family

ID=60953318

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17828247.1A Pending EP3481945A4 (de) 2016-07-11 2017-07-10 Rekombinante polypeptide mit selenocystein und verfahren zur herstellung davon

Country Status (6)

Country Link
US (1) US11155804B2 (de)
EP (1) EP3481945A4 (de)
JP (2) JP7269648B2 (de)
CN (1) CN109844105A (de)
CA (1) CA3030163A1 (de)
WO (1) WO2018013481A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11492650B2 (en) * 2016-08-30 2022-11-08 Board Of Regents, The University Of Texas System Production of seleno-biologics in genomically recoded organisms
CN109486802B (zh) * 2018-12-26 2020-08-04 广州白云山拜迪生物医药有限公司 一种酶活提高的青霉素酶突变体及其构建方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012657A2 (en) * 1999-08-16 2001-02-22 Karolinska Innovations Ab Methods and means for selenoprotein expression
WO2013009868A1 (en) * 2011-07-11 2013-01-17 Yale University Compositions and methods for making selenocysteine containing polypeptides
US20160060301A1 (en) * 2014-08-30 2016-03-03 Northwestern University Methods for improved in vitro protein synthesis with proteins containing non standard amino acids

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10309193A (ja) 1997-05-12 1998-11-24 Sankyo Co Ltd 哺乳類セレノシステイン含有タンパク質の大腸菌での製造法
US6849417B1 (en) 1998-04-06 2005-02-01 The United States Of America As Represented By The Department Of Health And Human Services Mammalian selenoprotein differentially expressed in tumor cells
EP1437410A4 (de) 2001-09-13 2005-01-26 Juridical Foundation Neue selenocystein enthaltende proteine
WO2007075438A2 (en) 2005-12-15 2007-07-05 Codon Devices, Inc. Polypeptides comprising unnatural amino acids, methods for their production and uses therefor
WO2008140477A2 (en) 2006-11-02 2008-11-20 Capon Daniel J Hybrid immunoglobulins with moving parts
WO2009043051A2 (en) 2007-09-27 2009-04-02 Biogen Idec Ma Inc. Cd23 binding molecules and methods of use thereof
DK2217265T3 (en) 2007-11-20 2017-07-03 Ambrx Inc Modified insulin polypeptides and their use
RU2013139260A (ru) 2011-01-28 2015-03-10 Нэшнл Рисеч Каунсил Оф Канада Конструирование иммуноглобулиновых доменов
WO2012170969A2 (en) 2011-06-10 2012-12-13 Biogen Idec Ma Inc. Pro-coagulant compounds and methods of use thereof
US10876142B2 (en) 2011-07-11 2020-12-29 Yale University Compositions and methods for making selenocysteine containing polypeptides
US10240158B2 (en) 2011-07-11 2019-03-26 Yale University Compositions and methods for making selenocysteine containing polypeptides
WO2014153164A1 (en) 2013-03-14 2014-09-25 The California Institute For Biomedical Research Targeting agent antibody conjugates and uses thereof
US20150050682A1 (en) 2013-08-15 2015-02-19 University Of Vermont And State Agricultural College Direct assay of thioredoxin reductase activity
US10519247B2 (en) 2013-11-01 2019-12-31 Board Of Regents,The University Of Texas System Targeting HER2 and HER3 with bispecific antibodies in cancerous cells
WO2016172269A2 (en) 2015-04-20 2016-10-27 University Of Utah Research Foundation Insulin analogs having shortened b chain peptides and associated methods
US10557160B2 (en) 2015-12-15 2020-02-11 Board Of Regents, The University Of Texas System Transgenic bacteria with expanded amino acid usage and nucleic acid molecules for use in the same
CA3030930A1 (en) 2016-07-22 2018-01-25 University Of Utah Research Foundation Insulin analogs
US11492650B2 (en) 2016-08-30 2022-11-08 Board Of Regents, The University Of Texas System Production of seleno-biologics in genomically recoded organisms

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012657A2 (en) * 1999-08-16 2001-02-22 Karolinska Innovations Ab Methods and means for selenoprotein expression
WO2013009868A1 (en) * 2011-07-11 2013-01-17 Yale University Compositions and methods for making selenocysteine containing polypeptides
US20160060301A1 (en) * 2014-08-30 2016-03-03 Northwestern University Methods for improved in vitro protein synthesis with proteins containing non standard amino acids

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE ENA [online] 10 April 2013 (2013-04-10), "Escherichia coli p0305293.4 cysteine synthase A", XP002796700, retrieved from EBI Database accession no. ENG64632 *
DATABASE ENA [online] 12 May 2016 (2016-05-12), "Escherichia coli glutamyl tRNA reductase", XP002796709, retrieved from EBI Database accession no. KJJ77964 *
DATABASE UniProt [online] 20 May 2008 (2008-05-20), "RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094}; Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};", XP002796711, retrieved from EBI accession no. UNIPROT:B1ITB1 Database accession no. B1ITB1 *
DATABASE UniProt [online] 25 January 2012 (2012-01-25), "RecName: Full=Aerobic respiration control sensor protein {ECO:0000256|PIRNR:PIRNR003182}; EC=2.7.13.3 {ECO:0000256|PIRNR:PIRNR003182};", XP002796708, retrieved from EBI accession no. UNIPROT:G5QQB8 Database accession no. G5QQB8 *
DATABASE UniProt [online] 28 June 2011 (2011-06-28), "RecName: Full=7-carboxy-7-deazaguanine synthase {ECO:0000256|HAMAP-Rule:MF_00917}; Short=CDG synthase {ECO:0000256|HAMAP-Rule:MF_00917}; EC=4.3.99.3 {ECO:0000256|HAMAP-Rule:MF_00917}; AltName: Full=Queuosine biosynthesis protein QueE {ECO:0000256|HAMAP-Rule:MF_00917};", XP002796710, retrieved from EBI accession no. UNIPROT:F4VHX6 Database accession no. F4VHX6 *
O. RENGBY ET AL: "Titration and Conditional Knockdown of the prfB Gene in Escherichia coli: Effects on Growth and Overproduction of the Recombinant Mammalian Selenoprotein Thioredoxin Reductase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 2, 1 January 2007 (2007-01-01), US, pages 432 - 441, XP055654785, ISSN: 0099-2240, DOI: 10.1128/AEM.02019-06 *
T. K. MCDANIEL ET AL: "A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 92, no. 5, 28 February 1995 (1995-02-28), pages 1664 - 1668, XP055654992, ISSN: 0027-8424, DOI: 10.1073/pnas.92.5.1664 *

Also Published As

Publication number Publication date
CA3030163A1 (en) 2018-01-18
EP3481945A1 (de) 2019-05-15
CN109844105A (zh) 2019-06-04
JP7269648B2 (ja) 2023-05-09
JP2023060001A (ja) 2023-04-27
WO2018013481A1 (en) 2018-01-18
US20190316110A1 (en) 2019-10-17
US11155804B2 (en) 2021-10-26
JP2019520083A (ja) 2019-07-18

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