EP3480319A4 - METHOD OF PRODUCING DNA BANK AND METHOD OF ANALYZING GENOMIC DNA USING DNA BANK - Google Patents

METHOD OF PRODUCING DNA BANK AND METHOD OF ANALYZING GENOMIC DNA USING DNA BANK Download PDF

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Publication number
EP3480319A4
EP3480319A4 EP17819589.7A EP17819589A EP3480319A4 EP 3480319 A4 EP3480319 A4 EP 3480319A4 EP 17819589 A EP17819589 A EP 17819589A EP 3480319 A4 EP3480319 A4 EP 3480319A4
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EP
European Patent Office
Prior art keywords
dna
dna library
producing
library
analyzing genomic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17819589.7A
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German (de)
English (en)
French (fr)
Other versions
EP3480319A1 (en
Inventor
Hiroyuki Enoki
Yoshie Takeuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyota Motor Corp
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Toyota Motor Corp
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Publication date
Application filed by Toyota Motor Corp filed Critical Toyota Motor Corp
Priority claimed from PCT/JP2017/013965 external-priority patent/WO2018003220A1/ja
Publication of EP3480319A1 publication Critical patent/EP3480319A1/en
Publication of EP3480319A4 publication Critical patent/EP3480319A4/en
Pending legal-status Critical Current

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/179Modifications characterised by incorporating arbitrary or random nucleotide sequences
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/204Modifications characterised by specific length of the oligonucleotides
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    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/143Concentration of primer or probe
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    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
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    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/125Electrophoretic separation
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP17819589.7A 2016-06-29 2017-04-03 METHOD OF PRODUCING DNA BANK AND METHOD OF ANALYZING GENOMIC DNA USING DNA BANK Pending EP3480319A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2016129048 2016-06-29
JP2016178528 2016-09-13
JP2017071020A JP7343264B2 (ja) 2016-06-29 2017-03-31 Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法
PCT/JP2017/013965 WO2018003220A1 (ja) 2016-06-29 2017-04-03 Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法

Publications (2)

Publication Number Publication Date
EP3480319A1 EP3480319A1 (en) 2019-05-08
EP3480319A4 true EP3480319A4 (en) 2019-05-08

Family

ID=61693451

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17819589.7A Pending EP3480319A4 (en) 2016-06-29 2017-04-03 METHOD OF PRODUCING DNA BANK AND METHOD OF ANALYZING GENOMIC DNA USING DNA BANK

Country Status (11)

Country Link
US (1) US20190233889A1 (ja)
EP (1) EP3480319A4 (ja)
JP (1) JP7343264B2 (ja)
KR (1) KR102298586B1 (ja)
CN (1) CN109715798B (ja)
AU (1) AU2017287059B2 (ja)
CA (1) CA3029167C (ja)
IL (1) IL263960A (ja)
MX (1) MX2018015860A (ja)
PH (1) PH12018502739A1 (ja)
SG (1) SG11201811735WA (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106676099B (zh) * 2016-12-21 2019-07-02 中国水稻研究所 构建简化基因组文库的方法及试剂盒
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたdnaライブラリーの作製方法
JP7047373B2 (ja) 2017-12-25 2022-04-05 トヨタ自動車株式会社 次世代シーケンサー用プライマー並びにその製造方法、次世代シーケンサー用プライマーを用いたdnaライブラリー並びにその製造方法、及びdnaライブラリーを用いたゲノムdna解析方法
CN110256566A (zh) * 2019-05-10 2019-09-20 江苏苏博生物医学科技南京有限公司 Taq DNA聚合酶单链免疫球蛋白IgG抗体、制备方法及其在基因分型检测中的应用
JP7522431B2 (ja) 2020-04-01 2024-07-25 国立大学法人北海道大学 動物試料からのウイルス検出方法
WO2024159179A1 (en) * 2023-01-27 2024-08-02 Ultima Genomics, Inc. Systems and methods for nucleic acid mismatch error detection

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130085083A1 (en) * 2003-03-07 2013-04-04 Rubicon Genomics Substantially non-self complementary primers

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
JP4255630B2 (ja) 2001-09-10 2009-04-15 独立行政法人農業・食品産業技術総合研究機構 米のdna食味判定技術及び籾/玄米半粒による良食味米選抜方法
DE03808546T1 (de) * 2002-12-23 2006-01-26 Agilent Technologies, Inc., Palo Alto Vergleichende genomischehybridisierungstests unter verwendung von merkmalen immobilisierteroligonukleotide sowie zusammensetzungen zur durchführung davon
WO2004081225A2 (en) * 2003-03-07 2004-09-23 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US20050053980A1 (en) * 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
JP3972106B2 (ja) * 2004-03-03 2007-09-05 大学共同利用機関法人情報・システム研究機構 ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー
ES2645661T3 (es) 2006-04-04 2017-12-07 Keygene N.V. Detección de alto rendimiento de marcadores moleculares basada en fragmentos de restricción

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130085083A1 (en) * 2003-03-07 2013-04-04 Rubicon Genomics Substantially non-self complementary primers

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DIETMAIER W ET AL: "MULTIPLE MUTATION ANALYSES IN SINGLE TUMOR CELLS WITH IMPROVED WHOLE GENOME AMPLIFICATION", THE AMERICAN JOURNAL OF PATHOLOGY; [10640], ELSEVIER INC, US, vol. 154, no. 1, 1 January 1999 (1999-01-01), pages 83 - 95, XP001097963, ISSN: 0002-9440 *
PINARD ROBERT ET AL: "Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing", BMC GENOMICS, BIOMED CENTRAL LTD, LONDON, UK, vol. 7, no. 1, 23 August 2006 (2006-08-23), pages 216, XP021014593, ISSN: 1471-2164, DOI: 10.1186/1471-2164-7-216 *
See also references of WO2018003220A1 *
ZHANG L ET AL: "Whole genome amplification from single cell: Implications for genetic analysis", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 89, 1 July 1992 (1992-07-01), pages 5847 - 5851, XP002130909, ISSN: 0027-8424, DOI: 10.1073/PNAS.89.13.5847 *

Also Published As

Publication number Publication date
CN109715798A (zh) 2019-05-03
IL263960A (en) 2019-01-31
BR112018077489A2 (pt) 2019-09-03
MX2018015860A (es) 2019-03-28
CA3029167A1 (en) 2018-01-04
CA3029167C (en) 2022-06-21
EP3480319A1 (en) 2019-05-08
NZ749198A (en) 2021-01-29
JP7343264B2 (ja) 2023-09-12
SG11201811735WA (en) 2019-01-30
KR102298586B1 (ko) 2021-09-07
KR20190020338A (ko) 2019-02-28
AU2017287059B2 (en) 2021-06-10
US20190233889A1 (en) 2019-08-01
JP2018042548A (ja) 2018-03-22
PH12018502739A1 (en) 2019-09-30
CN109715798B (zh) 2023-04-28

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