EP3317392B1 - Polymeres entferntes verschlusssystem für einwegbehälter - Google Patents

Polymeres entferntes verschlusssystem für einwegbehälter Download PDF

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Publication number
EP3317392B1
EP3317392B1 EP16818476.0A EP16818476A EP3317392B1 EP 3317392 B1 EP3317392 B1 EP 3317392B1 EP 16818476 A EP16818476 A EP 16818476A EP 3317392 B1 EP3317392 B1 EP 3317392B1
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EP
European Patent Office
Prior art keywords
instrument
seal system
remote seal
pressure
coupling
Prior art date
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EP16818476.0A
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English (en)
French (fr)
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EP3317392A4 (de
EP3317392A1 (de
Inventor
Paul R. FADELL
Mark S. Schumacher
Joshua M. PRICE
Fred C. Sittler
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Rosemount Inc
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Rosemount Inc
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Publication of EP3317392A4 publication Critical patent/EP3317392A4/de
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
    • G01L19/00—Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
    • G01L19/0007—Fluidic connecting means
    • G01L19/0046—Fluidic connecting means using isolation membranes
    • G—PHYSICS
    • G08—SIGNALLING
    • G08C—TRANSMISSION SYSTEMS FOR MEASURED VALUES, CONTROL OR SIMILAR SIGNALS
    • G08C17/00—Arrangements for transmitting signals characterised by the use of a wireless electrical link
    • G08C17/02—Arrangements for transmitting signals characterised by the use of a wireless electrical link using a radio link
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
    • G01L19/00—Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
    • G01L19/0007—Fluidic connecting means
    • G01L19/003—Fluidic connecting means using a detachable interface or adapter between the process medium and the pressure gauge
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
    • G01L7/00—Measuring the steady or quasi-steady pressure of a fluid or a fluent solid material by mechanical or fluid pressure-sensitive elements
    • G01L7/02—Measuring the steady or quasi-steady pressure of a fluid or a fluent solid material by mechanical or fluid pressure-sensitive elements in the form of elastically-deformable gauges
    • G01L7/08—Measuring the steady or quasi-steady pressure of a fluid or a fluent solid material by mechanical or fluid pressure-sensitive elements in the form of elastically-deformable gauges of the flexible-diaphragm type

Definitions

  • Single-use containers such as bioreactors, are useful for generating and supporting biological reactions for any number of purposes.
  • Biological reactions can be susceptible to changes in temperature and/or pressure.
  • the reaction itself may change various parameters within the bioreactor, such as the pressure. Accordingly, it may be important to monitor pressure or other variables of the biological reaction.
  • the life sciences industry is moving from large, capital-intensive facilities made of stainless steel with large clean-in-place (CIP) infrastructure to smaller facilities that use polymeric bags or containers functioning as bioreactors.
  • CIP clean-in-place
  • the bioreactor bag is used once and then discarded.
  • This single-use bioreactor technique significantly reduces the capital cost of the plant. For example, in existing facilities that use stainless steel CIP infrastructure, up to 90% of the cost of operating the facility may be due to the clean-in-place infrastructure, including very high end instrumentation designed to withstand a steam cleaning cycle.
  • the CIP portion of the capital can be eliminated and the facility can be more flexible and much smaller, which, in turn, allows the production of the smaller batches that are needed for more targeted drug therapies and other smaller-scale applications.
  • WO20091202269 discloses (claims 1, 5 and 8, p.7, ⁇ 1) a method of interfacing a non-sterile sensor such as a pressure sensor to a sterile flow stream, of a bioreactor for example.
  • a non-sterile sensor such as a pressure sensor
  • a sterile flow stream of a bioreactor for example.
  • the process fluid inside the bioreactor bag and the sensor are separated by the separation membrane and the sensor, no polymeric remote seal is provided therebetween.
  • a polymeric remote seal system for coupling a single-use container to a pressure measurement instrument.
  • the polymeric remote seal system includes a process-side coupling, an instrument-side coupling and a fluidic coupling therebetween.
  • the process-side coupling is configured to couple to the single-use container and is formed of a radiation sterilizable polymer.
  • the process-side coupling has a process-side deflectable diaphragm that is configured to deflect in response to pressure of the single-use container.
  • the instrument-side coupling is configured to couple to the pressure measurement instrument and is formed of a radiation sterilizable polymer.
  • the instrument-side coupling is configured to fluidically convey fluid pressure to an isolation diaphragm of the pressure measurement instrument. Tubing fluidically couples the process-side coupling to the instrument-side coupling.
  • Embodiments of the present invention leverage a remote seal system in order to couple pressure within single-use container such as a bioreactor to a high-precision pressure measuring instrument. Accordingly, the actual sensor that measures the pressure within the bioreaction vessel is disposed within the high-precision pressure measurement instrument.
  • the remote seal system is formed of a polymeric material that is pre-sterilized, and, in some embodiments, physically coupled to a pre-sterilized single-use bioreactor. Accordingly, the bioreactor as well as the remote seal system itself are disposable. This allows the use of an accurate and precise reusable pressure transmitter but still provides the end user with a pre-sterilized connection to the bioreaction vessel.
  • the field instrument-side and the process-side couplings will be described separately below. Embodiments of the present invention include any combination of the various process-side configurations with any of the various instrument-side configurations.
  • FIG. 1 is a diagrammatic view of a polymeric remote seal system used with a single-use bioreactor in accordance with an embodiment of the present invention.
  • Bioreaction system 100 includes bioreactor 102 coupled to pressure measuring instrument 104 via fluidic coupling 106.
  • Bioreactor 102 generally includes an outer support container 108 that has a wall that is relatively solid such that it forms a shell for single-use bioreaction bag 110 disposed therein.
  • Outer shell 108 is generally matched to the dimensions and functionality of single-use bioreaction bag 110. However outer shell 108 is typically a reusable item.
  • Single-use bioreactor bag 110 is generally a polymeric bag that is configured to support a biological reaction occurring within sample 112.
  • Polymeric remote seal system 114 couples the pressure within single-use bioreactor bag 110 to pressure measuring instrument 104.
  • This coupling is a fluidic coupling such that pressure acting against a diaphragm disposed proximate process connection 116 generates movement of fluid within coupling 106 to cause associated movement at a diaphragm proximate instrument coupling 118.
  • Such movement conveys the fluid pressure from bioreactor bag 110 to a pressure sensor within instrument 104 such that the pressure can be measured very accurately.
  • instrument 104 generally includes characterization and/or calibration information in order to compensate for variations in temperature and/or other environmental variables.
  • instrument 104 may also perform diagnostics relative to the device itself and/or the biological reaction in order to provide additional information instead of simply reporting the pressure within single-use bioreactor bag 110. Further still, instrument 104 may also be configured to convey the pressure information to one or more additional devices via a process communication loop or segment, such as that in accordance with the Highway Addressable Remote Transducer (HART ® ) protocol or the FOUNDATION TM Fieldbus protocol. Moreover, embodiments described herein may also include wirelessly transmitting such pressure information to any suitable device via antenna 120 in accordance with a wireless process communication protocol, such as IEC62591. In one embodiment, instrument 104 is a commercially-available hygienic pressure transmitter sold under the trade designation Model 3051 HT available from Emerson Process Management of Shakopee, Minnesota.
  • Model 3051 HT available from Emerson Process Management of Shakopee, Minnesota.
  • FIG. 2A is a diagrammatic cross-sectional view of a process-side connection of a remote seal system to a single-use bioreactor in accordance with an embodiment of the present invention.
  • Flange 130 includes flange body 132 that is fluidically coupled to tubing 134.
  • a deformable isolation diaphragm 136 is coupled to flange body 132 in order to provide a fluid-tight seal.
  • flange body 132 is formed of a radiation-sterilizable polymer.
  • a radiation-sterilizable polymer is polyvinyl chloride. However any suitable radiation-sterilizable polymer can be used in accordance with embodiments of the present invention.
  • isolation diaphragm 136 is also formed of a radiation-sterilizable polymer of the same type as flange body 132.
  • Diaphragm 136 may be coupled to flange body 132 in accordance with various techniques. For example, diaphragm 136 may be welded to flange body 132 at annular weld 137 using known welding techniques such as thermal, ultrasonic, or a combination thereof. Accordingly, the plastic wall of single-use bioreactor will bear against isolation diaphragm 136 and pressure within the bioreactor will deform diaphragm 136 thereby forcing fill fluid through tubing 134.
  • the fill fluid can be any suitable fluid that is substantially incompressible at the pressures and temperatures of the application.
  • the fill fluid may be silicone oil, water, or any other suitable fluid. As set forth above, this will generate associated movement of a similar diaphragm at the pressure measuring instrument, which deflection is then measured or otherwise characterized by a high-accuracy, precision pressure sensor within instrument 104.
  • FIG. 2B is a diagrammatic cross-sectional view of a process-side coupling of a remote seal system in accordance with another embodiment of the present invention.
  • Remote seal system 140 includes a flange body 142 that is attached, such as via annular weld 145 to wall 144 of a single-use bioreactor bag. Accordingly, the embodiment illustrated with respect to FIG. 2B eliminates any seals and provides a simple connection for headspace and level measurements.
  • bag wall 144 functions to not only contain the bioreaction, but also to deflect in response to pressure therein such that the deflection of wall 144 causes movement of fluid within region 146, which ultimately causes fluid flow through tubing 134.
  • Flange body 142 is generally formed of a radiation-sterilizable polymer. However, the selection of material for flange body 142 can also be tailored such that it is readily weldable or otherwise attachable to materials that are commonly used for single-use bioreactors.
  • FIG. 2C is a diagrammatic cross-sectional view of a process-side coupling of a polymeric remote seal system in accordance with another embodiment of the present invention.
  • Process-side coupling 150 includes tubing connector 152 coupled to tubing 134.
  • Tubing connector 152 is generally formed of a radiation-sterilizable polymer.
  • Connector 152 includes one or more stepped portions 154, 156 that are configured to retain flexible tubing that is passed over the outside diameter of connector 152. In the embodiment shown in FIG. 2C , the flexible tubing that passes over stepped portions 154, 156 couples region 158 to the interior of the bioreactor.
  • Process-side coupling 150 includes a small collapsible bellows structure 159 that is attached, for example via weld 161, to connector 152.
  • bioreactor fluid pressure P acts upon surface 160 to collapse bellows 162 and thereby reduce the volume within region 164. This reduction in volume forces fluid therein through tubing 134, which fluid movement is detected as pressure by instrument 104.
  • FIG. 2D is a diagrammatic cross-sectional view of a process-side coupling of a polymeric remote seal system in accordance with another embodiment of the present invention.
  • Coupling 170 includes tubing connector 172 that is, in one embodiment, formed of a radiation-sterilizable polymer.
  • Connector 172 includes at least one stepped portion 174 that is configured to retain tubing 134 as tubing 134 is slipped over portion 174.
  • connector 172 includes another stepped portion 176 sized and configured to receive and retain tubing 178 which is coupled to a single-use bioreactor.
  • Connector 172 includes a deflectable isolation diaphragm 180 attached, such as by thermal or ultrasonic welding, to connector 172 at location 182. Accordingly, pressure within tubing 178 acts upon diaphragm 180 to cause movement of diaphragm 180 which changes the volume within region 184 thereby causing fluid flow through tubing 134.
  • FIG. 3A is a cross-sectional diagrammatic view of an instrument-side coupling of a polymeric remote seal system in accordance with an embodiment of the present invention.
  • Coupling 200 includes flange 202 attached or otherwise coupled to instrument 104 via any suitable methods, such as clamping, bolts, or any combination thereof.
  • a seal 204 is sandwiched between flange 202 and instrument 104 in order to create a fluid-tight chamber 206 therein.
  • Chamber 206 is bounded, on one side, by deflectable isolation diaphragm 208 of instrument 104. Accordingly, deflection of diaphragm 208 will cause an associated deflection of a measuring diaphragm or structure of a pressure sensor within instrument 104 such that the pressure is detectable.
  • Tubing 134 that is operably coupled to a single-use container such as bioreaction vessel is attached to flange 202.
  • Flange 202 is, in one embodiment, formed of a radiation-sterilizable polymer.
  • the remote seal system is filled with a substantially incompressible fluid prior to use. Accordingly, a syringe, such as syringe 210 is inserted into flange 202 at port 212. Port 212 is valved, using tubing valve 214, while a diaphragm pump or other suitable device is operably coupled to flange body valve 216.
  • the first step of filling the remote seal system with fluid is to evacuate air from the disposable process connection.
  • valve 216 is opened in order to evacuate all of the air from the system.
  • valve 216 is closed and tubing valve 214 is opened.
  • a predetermined volume of fill fluid is pushed into the remote seal system using syringe 210.
  • valve 214 is closed.
  • the pressure measuring instrument can be zeroed and the system is ready for use.
  • the disposable remote seal system can be removed, the refill assembly can be drained, and a new process connection can be installed for the next application.
  • FIG. 3B is a diagrammatic cross-sectional view of an instrument-side coupling of a polymeric remote seal system in accordance with another embodiment of the present invention.
  • Coupling 230 includes a flange body 232 that, in one embodiment, is formed of a radiation-sterilizable polymer.
  • Coupling 230 also includes a deformable polymeric membrane 234 operably coupled to flange body 232.
  • Deformable polymeric membrane 234 is also, in one embodiment, formed of a radiation-sterilizable polymer.
  • Membrane 234 may be coupled to flange body 232 in accordance with any suitable techniques. In one example, membrane 234 is welded to flange body 232.
  • embodiments of the present invention can include a polymeric remote seal system that is pre-filled with fill fluid such that no filling is required by the user prior to use.
  • embodiments of the present invention also include structures that allow a user thereof to fill the remote seal system with fill fluid prior to use.
  • flange body 232 is urged against instrument 104 to such an extent that seal 236 forms a fluid-tight connection.
  • fluid pressure received through tubing 134 causes movement of deformable polymeric membrane 234, which causes similar movement of membrane 238 of instrument 104. Movement of membrane 238 conveys fluid pressure to a high accuracy pressure sensor within instrument 104 in order to accurately measure the pressure.
  • FIG. 3C is a diagrammatic cross-sectional view of an instrument-side coupling of a polymeric remote seal system in accordance with another embodiment of the present invention.
  • Coupling 250 includes plastic body 252 coupled to tubing 134 such that bioreaction fluid pressure is conveyed through fill fluid within tubing 134 into chamber 254.
  • a deformable polymeric diaphragm 256 is sealingly coupled to plastic body 232 such that fluid pressure within region 254 generates a deflection of plastic diaphragm 256.
  • diaphragm 256 can be attached to flange 252 in a variety of ways, including ultrasonic or thermal welding. As indicated in FIG. 3C , flange 252 is urged against instrument 104 by instrument flange 258.
  • Instrument flange 258 includes an aperture 260 that is sized to pass flange 252.
  • flange 252 includes a shoulder 261 that receives the clamping pressure of instrument flange 258 and bears against seal 262.
  • a fluid tight seal is generated when instrument flange 258 is urged against shoulder 261.
  • One way in which is bias is accomplished is via mounting bolts, clamps, or the like.
  • movement of diaphragm 256 When so coupled, movement of diaphragm 256 generates an associated movement of the isolation diaphragm of instrument 104 which movement is then detected or otherwise measured by instrument 104 in order to provide a high quality pressure measurement.
  • embodiments of the present invention have generally provided a direct fluidic coupling extending from a bioreactor all the way to a high-precision process fluid pressure measurement instrument.
  • embodiments of the present invention can also leverage known remote seal systems in order to reduce the amount of material employed for the disposable portion of the remote seal system.
  • FIG. 4 is a diagrammatic cross-sectional view of a process-side coupling of a polymeric remote seal system in accordance with another embodiment of the present invention.
  • Coupling 400 includes seal body 402 which, in one embodiment, is formed of a radiation-sterilizable polymer.
  • Body 402 has a deflectable diaphragm 404 that is also, in one embodiment, formed of a radiation-sterilizable polymer.
  • Diaphragm 404 is attached, via welding, or other suitable techniques, to body 402.
  • Body 402 also includes a tubing connector 406 that is configured to receive tubing 134.
  • a process coupler 408 is operably coupleable to a single-use bioreactor via any suitable techniques.
  • Coupler 408 includes mounting region 410 that receives seal ring 412.
  • seal ring 412 has a pair of annular projections 414, 416 that extend into respective grooves 418, 420 in coupler 408 and body 402, respectively.
  • Each of coupler 408 and body 402 have tapered portions 422, 424, respectively, such that a circumferential clamp encircling portions 422, 424 generates a force biasing coupler 408 and body 402 together. In this way, a fluid-tight seal is generated between coupler 408 and body 402.
  • Body 402 also, in one embodiment, includes fill port 426 that allows fill fluid to be introduced into body 402 prior to use.
  • a single-use bioreactor can be provided with coupler 400 shown in FIG. 4 without any fluid present in the system.
  • bioreactor/coupler system can be pre-sterilized using radiation prior to use. Then, the user need only introduce fill fluid into port 426 and couple the system to a process fluid pressure measurement instrument in order to monitor the pressure of the bioreaction.
  • fluid can be introduced into port 426 without destroying the sterilization since the fluid introduced in port 426 is on an opposite side of plastic diaphragm 404 from the sterilized interior of the bioreactor bag.
  • FIG. 5 is a flow diagram of a method of using a polymeric remote seal system to monitor bioreaction pressure in accordance with an embodiment of the present invention.
  • Method 500 begins at block 502 where the polymeric remote seal system is coupled to a bioreactor bag.
  • the bioreactor bag/remote seal system is sterilized. In one embodiment, such sterilization employs a radiation process, such as a gamma radiation process.
  • the sterilized bioreactor/remote seal system is operably coupled to a pressure measurement instrument, such as that illustrated with respect to FIG. 1 .
  • method 500 may proceed directly to block 510 where the bioreaction sample is introduced into the bioreaction bag.
  • block 508 is executed to provide the requisite fill fluid.
  • one way in which the fill fluid can be introduced is by first evacuating the remote seal system and then coupling the evacuated remote seal system to a source of fill fluid, such as via a syringe or other suitable instrument.
  • the process measurement instrument is employed to measure the pressure within the bioreaction bag. This pressure may be measured continuously, periodically, intermittently, or in response to certain events.
  • method 500 continues to block 514 where the polymeric remote seal system is decoupled from the instrument and discarded.
  • various embodiments of the present invention employ a polymeric remote seal system that is filled (either on-site, or pre-filled) with a fill fluid.
  • the polymeric remote seal system can be made of plastic that can be sterilized.
  • the seal system allows the use of an accurate, and relatively expensive, pressure measuring instrument. However, while the remote seal system is disposable, the pressure measuring instrument is reused.
  • the polymeric remote seal system provides the user with a pre-sterilized connection to the process and a connection to the pressure measuring instrument. The polymeric remote seal system can be removed from the pressure measuring instrument and disposed of when the single-use bioreactor is disposed.
  • Both sides of the polymeric remote seal system may use similar constructions. Both sides will typically have a polymeric membrane with a gas permeation-inhibiting layer that is bonded to a polymeric seal. Fluid pressure from the process connection is passed through the fluid-filled system to the pressure measuring instrument.
  • the process-side connection generally includes a plastic body that has a membrane bonded thereto which connects with a bioreactor bag or vessel.
  • the instrument-side connection also generally includes a polymeric body with a membrane bonded thereto that passes the pressure to the instrument.
  • the system can be filled with a variety of pressure transmission media and uses either a fill screw or plastic-welded fill connection.
  • the system can be decoupled from the instrument and disposed of along with the single-use bioreactor.
  • a new fluid-filled polymeric remote seal system can then be attached to the instrument and connected to a new single-use bioreaction bag.
  • Embodiments of the present invention have, thus far, generally been described with respect to a polymeric remote seal system being used in conjunction with a bioreactor. However, embodiments of the present invention are practicable with any single-use container. Another example of a single use container is an intermediate bulk container or chemical tote.
  • FIG. 6 is a diagrammatic view of a polymeric remote seal system being used on an intermediate bulk container (IBC) in accordance with an embodiment of the present invention.
  • IBC 600 contains a quantity of liquid that may be dispensed from port 602 using valve 604.
  • IBC 600 is coupled to polymeric remote seal system 606 such that the pressure of the liquid within IBC 600 acts against a polymeric isolation diaphragm of system 606 to fluidically convey the fluid pressure to measuring instrument 608.
  • polymeric remote seal system 606 includes optional temperature sensor 610 disposed in thermal contact with the contents of IBC 600.
  • Temperature sensor 600 may be any suitable device that has an electrical characteristic that varies with temperature.
  • Temperature sensor 610 is electrically coupled to measuring instrument 608 via two or more conductors illustrated diagrammatically in phantom at reference numeral 612. Accordingly, in some embodiments, measuring instrument is able to measure not only pressure, but temperature as well. Additionally, in one embodiment, measuring instrument 608 can be provided with an indication of liquid density of the contents of IBC 608. Once liquid density is known, measuring instrument 608 is able to provide an indication of liquid level within IBC 608 based on the measured pressure and the known density.
  • measuring instrument 608 may, in some embodiments, provide a local indication relative to the contents of the single-use container to which it is coupled.
  • a face 614 is provided with an indicator, such as a needle 616 that provides information relative to the contents.
  • indicator 616 may provide a local indication of liquid level within IBC 600.
  • other variables such as pressure and/or temperature of the contents may be displayed on face 614.
  • Measuring instrument 608 also, in some embodiments, provides a digital indication of one or more variable to other devices wirelessly, as indicated at reference numeral 618.
  • FIG. 7 is a method of using a polymeric remote seal system with an intermediate bulk container in accordance with an embodiment of the present invention.
  • Method 700 begins at block 702 where a polymeric remote seal system is used to couple a single-use container to a measuring instrument, such as instrument 608.
  • the polymeric remote seal system includes a temperature sensor
  • the temperature sensor is electrically couple to the measuring instrument, as indicated at block 704.
  • the measuring instrument can receive liquid density information
  • optional block 706 is executed.
  • the liquid density information is received by the measuring instrument. This provision of density information to the measuring instrument can be locally at the measuring device, as indicated at reference numeral 708 or via wireless communication with the measuring instrument, as indicated at reference numeral 710.
  • the measuring instrument measures pressure and optionally temperature of the contents of the single-use container.
  • one or more outputs related to the measurements are provided.
  • the output(s) can be provided locally, as indicated at reference numeral 716 and/or wirelessly as indicated at reference numeral 718.
  • the output(s) provided at block 714 may be the measured pressure, a calculated level based on the pressure and known density, the temperature or any combination thereof.
  • the local output 716 and/or wireless output 718 can include alarm indications if, for example, the level is below a threshold or the temperature is above a threshold.
  • Embodiments described herein provide a convenient way to measure variables within a single-use container while still ensuring that high quality measurements are used.
  • the polymeric remote seal system can be disposed of or left with the single-use container once the container is empty or replaced. This allows the use and re-use of an accurate, complex wireless measurement instrument with different single-use containers without cleaning as the instrument is isolated from the single-use container.
  • the polymeric remote seal system can be disconnected from the measuring instrument or gauge and disposed or left with the single-use container. Then, a new polymeric remote seal system can be placed in the next single-use container and coupled to the same measuring instrument or gauge.

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Claims (14)

  1. Polymeres Fernabdichtungssystem zum Verbinden eines Einwegbehälters mit einem Druckmessinstrument, das System Folgendes umfassend:
    ein prozessseitiges Verbindungsstück konfiguriert, mit dem Einwegbehälter verbunden zu werden, wobei das prozessseitige Verbindungsstück aus einem strahlungssterilisierbaren Polymer gebildet ist und eine prozessseitige auslenkbare Membran aufweist, die konfiguriert ist, in Reaktion auf Druck des Einwegbehälters auszulenken;
    ein instrumentenseitiges Verbindungsstück konfiguriert, mit dem Druckmessinstrument verbunden zu werden, wobei das instrumentenseitige Verbindungsstück aus einem strahlungssterilisierbaren Polymer gebildet ist und konfiguriert ist, fluidisch Fluiddruck auf eine Isolationsmembran des Druckmessinstruments zu übertragen; und
    Schläuche, die prozessseitiges Verbindungsstück mit instrumentenseitigem Verbindungsstück fluidisch verbinden.
  2. Polymeres Fernabdichtungssystem nach Anspruch 1, wobei das prozessseitige Verbindungsstück konfiguriert ist, mit den Einwegbehälter verbunden zu werden.
  3. Polymeres Fernabdichtungssystem nach Anspruch 2, wobei das prozessseitige Verbindungsstück einen mit dem Einwegbehälter verbindbaren Flanschkörper umfasst, wobei der Flanschkörper mit der prozessseitigen auslenkbaren Membran verschweißt ist.
  4. Polymeres Fernabdichtungssystem nach Anspruch 2, wobei das prozessseitige Verbindungsstück einen Flanschkörper umfasst, der mit einer Wand des Einwegbehälters verbunden ist, so dass ein Teil der Einwegbehälterwand die prozessseitige auslenkbare Membran bildet.
  5. Polymeres Fernabdichtungssystem nach Anspruch 2, wobei das prozessseitige Verbindungsstück einen Schlauchverbinder mit mindestens einem abgestuften Abschnitt aufweist, konfiguriert, einen mit dem Einwegbehälter verbundenen Schlauch zu halten, und wobei die prozessseitige auslenkbare Membran aus einer faltbaren Balgstruktur gebildet ist, die innerhalb des Schlauchverbinders angeordnet ist.
  6. Polymeres Fernabdichtungssystem nach Anspruch 1, wobei das instrumentenseitige Verbindungsstück einen Flansch umfasst, der mit dem Druckmessinstrument koppelbar ist, wobei der Flansch eine Vielzahl von Öffnungen darin aufweist, wobei jede Öffnung durch ein entsprechendes Ventil verschlossen ist.
  7. Polymeres Fernabdichtungssystem nach Anspruch 1, wobei das instrumentenseitige Verbindungsstück einen Flansch und eine instrumentenseitige verbundene, auslenkbare Membran aufweist, wobei Druck von dem Einwegbehälter bewirkt, dass die instrumentenseitige auslenkbare Membran des instrumentenseitigen Verbindungsstücks an der Isolationsmembran des Druckmessgeräts anliegt.
  8. Polymeres Fernabdichtungssystem nach Anspruch 7, wobei die instrumentenseitige auslenkbare Membran mit dem Flansch verschweißt ist.
  9. Polymeres Fernabdichtungssystem nach Anspruch 7, wobei das polymere Fernabdichtungssystem mit Füllflüssigkeit vorgefüllt ist, die das prozessseitige Verbindungsstück mit dem geräteseitigen Verbindungsstück fluidisch verbindet.
  10. Verfahren zum fluidischen Verbinden von Druck eines Bioreaktors als Einwegbehälter mit einem Druckmessinstrument, das Verfahren umfassend:
    Bereitstellen eines polymeren Fernabdichtungssystems mit einem prozessseitigen Anschluss und einem instrumentenseitigen Anschluss;
    Verbinden des prozessseitigen Anschlusses mit dem Bioreaktor;
    Sterilisieren des Bioreaktors und zumindest des prozessseitigen Anschlusses;
    Verbinden des instrumentenseitigen Anschlusses mit dem Druckmessgerät, um fluidisch Fluiddruck auf eine Isolationsmembran des Druckmessgeräts zu übertragen;
    Einführen einer Probe in den Bioreaktor;
    Verwenden des Druckmessinstruments, um Druck innerhalb des Bioreaktors zu messen; und Füllen des polymeren Fernabdichtungssystems mit inkompressibler Füllflüssigkeit nach dem Verbinden des instrumentenseitigen Anschlusses mit dem Druckmessinstrument.
  11. Verfahren nach Anspruch 10 und ferner umfassend Lösen der Verbindung des polymeren Fernabdichtungssystems von dem Druckmessgerät und gemeinsames Verwerfen des Bioreaktors und des polymeren Fernabdichtungssystems.
  12. Flüssigkeitsmesssystem, umfassend:
    ein polymeres Fernabdichtungssystem enthaltend:
    ein prozessseitiges Verbindungsstück konfiguriert, mit einem Einwegbehälter verbunden zu werden, wobei das prozessseitige Verbindungsstück aus einem Polymer gebildet ist und eine prozessseitige auslenkbare Membran aufweist, die konfiguriert ist, sich in Reaktion auf Druck des Einwegbehälters auszulenken;
    ein instrumentenseitiges Verbindungsstück, das mit dem prozessseitigen Verbindungsstück über einen mit einem Isolationsfluid gefüllten Schlauch fluidisch verbunden ist, wobei das instrumentenseitige Verbindungsstück konfiguriert ist, mit einem Messinstrument gekoppelt zu werden, wobei das instrumentenseitige Verbindungsstück aus einem Polymer gebildet ist und konfiguriert ist, fluidisch Fluiddruck zu dem Messinstrument zu übertragen; und
    ein mit dem polymeren Fernabdichtungssystem verbundenes Messinstrument, wobei das Messinstrument eine Isolationsmembran aufweist, die fluidisch mit dem instrumentenseitigen Verbindungsstück des polymeren Fernabdichtungssystems verbunden und konfiguriert ist ein Ausgangssignal basierend auf von dem instrumentenseitigen Verbindungsstück übertragenen Druck zu liefern.
  13. Flüssigkeitsmesssystem nach Anspruch 12, wobei das polymere Fernabdichtungssystem einen mit dem Messinstrument verbundenen Temperatursensor umfasst.
  14. Flüssigkeitsmesssystem nach Anspruch 12, wobei das Messinstrument eine Anzeige des Flüssigkeitspegels innerhalb des Einwegbehälters basierend auf gemessenem Druck und bekannter Flüssigkeitsdichte liefert.
EP16818476.0A 2015-06-30 2016-06-21 Polymeres entferntes verschlusssystem für einwegbehälter Active EP3317392B1 (de)

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US14/788,069 US9562819B2 (en) 2015-06-30 2015-06-30 Polymeric remote seal system for single-use containers
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AU2016288175B2 (en) 2018-11-08
EP3317392A4 (de) 2019-02-20
MX2017017121A (es) 2018-03-09
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