EP3286296A4 - Microfluidic cell culture - Google Patents

Microfluidic cell culture Download PDF

Info

Publication number
EP3286296A4
EP3286296A4 EP16783924.0A EP16783924A EP3286296A4 EP 3286296 A4 EP3286296 A4 EP 3286296A4 EP 16783924 A EP16783924 A EP 16783924A EP 3286296 A4 EP3286296 A4 EP 3286296A4
Authority
EP
European Patent Office
Prior art keywords
cell culture
microfluidic cell
microfluidic
culture
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP16783924.0A
Other languages
German (de)
French (fr)
Other versions
EP3286296A1 (en
Inventor
Randall D. LOWE, Jr.
Kristin BEAUMONT
Aathavan KARUNAKARAN
Natalie MARKS
Jason M. MCEWEN
Mark P. White
J. Tanner Nevill
Gang F. WANG
Andrew W. Mcfarland
Daniele Malleo
Keith J. Breinlinger
Xiao GUAN
Kevin T. Chapman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bruker Cellular Analysis Inc
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Priority claimed from PCT/US2016/028808 external-priority patent/WO2016172454A1/en
Publication of EP3286296A1 publication Critical patent/EP3286296A1/en
Publication of EP3286296A4 publication Critical patent/EP3286296A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP16783924.0A 2015-04-22 2016-04-22 Microfluidic cell culture Pending EP3286296A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562151325P 2015-04-22 2015-04-22
PCT/US2016/028808 WO2016172454A1 (en) 2015-04-22 2016-04-22 Microfluidic cell structure

Publications (2)

Publication Number Publication Date
EP3286296A1 EP3286296A1 (en) 2018-02-28
EP3286296A4 true EP3286296A4 (en) 2019-01-09

Family

ID=58055846

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16783924.0A Pending EP3286296A4 (en) 2015-04-22 2016-04-22 Microfluidic cell culture

Country Status (4)

Country Link
EP (1) EP3286296A4 (en)
CN (2) CN114085730A (en)
HK (1) HK1253068A1 (en)
TW (2) TWI744230B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3576814A4 (en) * 2017-01-31 2020-02-19 Alphinity, LLC Bioprocess vessels with integrated pump
CN108485972B (en) * 2018-03-28 2021-06-25 东南大学 Microfluidic chip for cell tissue culture and real-time monitoring and use method thereof
WO2020014664A1 (en) * 2018-07-12 2020-01-16 Berkeley Lights, Inc. Screening plant protoplasts for disease resistant traits
CN114126762B (en) * 2019-04-30 2023-01-03 伯克利之光生命科技公司 Methods for encapsulating and assaying cells
WO2021038996A1 (en) * 2019-08-29 2021-03-04 ファナック株式会社 Cell production device and cell production method
CA3155946A1 (en) * 2019-10-25 2021-04-29 Angel Navas ANGELES Systems for operating microfluidic devices
JP7339138B2 (en) * 2019-12-02 2023-09-05 株式会社日立製作所 Cell culture device
US20220348863A1 (en) * 2021-04-28 2022-11-03 A.U. Vista, Inc. Tft-based cell isolation device and cell manipulation panel thereof
CN113996355B (en) * 2021-10-28 2023-06-06 上海浚真生命科学有限公司 Sampling device
TWI832371B (en) * 2022-08-10 2024-02-11 醫華生技股份有限公司 Biological particle culture apparatus and microstructure thereof
TWI849811B (en) * 2023-03-21 2024-07-21 好孕行生醫股份有限公司 Heating culture device for providing vertical temperature difference environment and use method thereof
TWI848840B (en) * 2023-10-25 2024-07-11 財團法人金屬工業研究發展中心 Cell modification system and device
CN117262436B (en) * 2023-11-21 2024-01-26 山东大学齐鲁医院 Tumor section storage device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226012A1 (en) * 2005-04-08 2006-10-12 Kanagasabapathi Thirukumaran T Integrated microfluidic transport and sorting system
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
WO2016090295A1 (en) * 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground

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TW550066B (en) * 2000-12-01 2003-09-01 Ind Tech Res Inst Micro bio-cultivation device
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US6984485B2 (en) * 2002-04-23 2006-01-10 Beckman Coulter, Inc. Polymer-coated substrates for immobilization of biomolecules and cells
US7790443B2 (en) * 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
US20080257735A1 (en) * 2004-09-24 2008-10-23 Regents Of The University Of California Microfluidic Device for Enabling the Controlled Growth of Cells and Methods Relating to Same
US20060252087A1 (en) * 2005-01-18 2006-11-09 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
US8475886B2 (en) * 2005-08-05 2013-07-02 Corning Incorporated Methods for producing surfaces that resist non-specific protein binding and cell attachment
US8148111B2 (en) * 2006-03-17 2012-04-03 Sanyo Chemical Industries, Ltd. Cell culture carrier comprising poly(meth)acrylic (salt) particle and artificial polypeptide
KR100903969B1 (en) * 2007-06-12 2009-06-25 삼성전자주식회사 Microarray, substrate for microarray and methods of fabricating the same
CN101275114A (en) * 2008-04-22 2008-10-01 北京大学 Microflow cell culture array and application thereof
TW201144805A (en) * 2010-06-08 2011-12-16 Academia Sinica Microfluidic device
CN102296029B (en) * 2010-06-28 2013-01-30 裴国献 Perfusion bioreactor system
US20140154703A1 (en) * 2011-01-06 2014-06-05 Alison Skelley Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
EP2664581B1 (en) * 2011-01-11 2019-10-09 Nippon Sheet Glass Company, Limited Flake-like mesoporous particles and manufacturing method therefor
WO2012109068A2 (en) * 2011-02-11 2012-08-16 Corning Incorporated Enzyme cleavable cell release polymeric surface
JP6122388B2 (en) * 2011-02-28 2017-04-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Cell culture system
TWI438273B (en) * 2011-03-08 2014-05-21 Univ Chang Gung High-throughput perfusative microfluidic cell culture wafers for miniaturized three-dimensional cell culture
TW201310016A (en) * 2011-08-26 2013-03-01 Chin-Feng Wan Biochip and fabricating method thereof
US10626435B2 (en) * 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
CN204079986U (en) * 2014-05-30 2015-01-07 南京农业大学 A kind of micro-nano-fluidic control device for cell migration research

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226012A1 (en) * 2005-04-08 2006-10-12 Kanagasabapathi Thirukumaran T Integrated microfluidic transport and sorting system
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
WO2016090295A1 (en) * 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2016172454A1 *

Also Published As

Publication number Publication date
CN114085730A (en) 2022-02-25
TW202233824A (en) 2022-09-01
EP3286296A1 (en) 2018-02-28
TWI819383B (en) 2023-10-21
HK1253068A1 (en) 2019-06-06
CN107849505A (en) 2018-03-27
CN107849505B (en) 2021-09-07
TWI744230B (en) 2021-11-01
TW201643241A (en) 2016-12-16

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