EP3208333A4 - Verfahren zum nachweis und zur quantifizierung von zielnukleinsäuren in einer testprobe mit einer neuartigen positive-control-nukleinsäure - Google Patents

Verfahren zum nachweis und zur quantifizierung von zielnukleinsäuren in einer testprobe mit einer neuartigen positive-control-nukleinsäure Download PDF

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Publication number
EP3208333A4
EP3208333A4 EP15850481.1A EP15850481A EP3208333A4 EP 3208333 A4 EP3208333 A4 EP 3208333A4 EP 15850481 A EP15850481 A EP 15850481A EP 3208333 A4 EP3208333 A4 EP 3208333A4
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EP
European Patent Office
Prior art keywords
nucleic acid
detecting
test sample
positive control
target nucleic
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EP15850481.1A
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English (en)
French (fr)
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EP3208333B1 (de
EP3208333A1 (de
Inventor
Norio Shimizu
Ken Watanabe
Yasuhiro TOMARU
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Tokyo Medical and Dental University NUC
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Tokyo Medical and Dental University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP15850481.1A 2014-10-17 2015-10-14 Verfahren zum nachweis und zur quantifizierung von zielnukleinsäuren in einer testprobe mit einer neuartigen positive-control-nukleinsäure Active EP3208333B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014212496 2014-10-17
PCT/JP2015/005198 WO2016059798A1 (ja) 2014-10-17 2015-10-14 新規な陽性コントロール核酸を利用した、被検試料中の標的核酸の検出・定量法

Publications (3)

Publication Number Publication Date
EP3208333A1 EP3208333A1 (de) 2017-08-23
EP3208333A4 true EP3208333A4 (de) 2018-03-21
EP3208333B1 EP3208333B1 (de) 2020-12-16

Family

ID=55746358

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15850481.1A Active EP3208333B1 (de) 2014-10-17 2015-10-14 Verfahren zum nachweis und zur quantifizierung von zielnukleinsäuren in einer testprobe mit einer neuartigen positive-control-nukleinsäure

Country Status (4)

Country Link
US (1) US20180087106A1 (de)
EP (1) EP3208333B1 (de)
JP (1) JP6778374B2 (de)
WO (1) WO2016059798A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3746565A4 (de) * 2018-01-30 2021-11-03 BioFire Defense, LLC Verfahren und systeme zur validierung eines nukleinsäureamplifizierungstests
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
JP7418717B2 (ja) * 2019-06-07 2024-01-22 国立大学法人 東京医科歯科大学 微生物の検出方法
IT201900008781A1 (it) * 2019-06-12 2020-12-12 AB Analitica S R L Metodo di amplificazione di acidi nucleici
JP7272202B2 (ja) * 2019-09-20 2023-05-12 株式会社島津製作所 標的核酸の検査方法および検査装置
JPWO2023027073A1 (de) * 2021-08-27 2023-03-02

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
US20070141563A1 (en) * 2005-12-21 2007-06-21 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2010111509A1 (en) * 2009-03-25 2010-09-30 Life Technologies Corporation Discriminatory positive/extraction control dna
EP2660332A1 (de) * 2012-05-01 2013-11-06 F. Hoffmann-La Roche AG Positives Steuerkonzept
WO2014063051A2 (en) * 2012-10-18 2014-04-24 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358679B1 (en) * 2000-08-24 2002-03-19 Pe Corporation (Ny) Methods for external controls for nucleic acid amplification
JP2004329209A (ja) * 2003-04-18 2004-11-25 Hitachi Ltd 遺伝子検出法及び遺伝子検出用プローブキット
JP2007130006A (ja) * 2005-11-11 2007-05-31 Gotaro Watanabe 遺伝子の塩基置換のリアルタイム検査法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
US20070141563A1 (en) * 2005-12-21 2007-06-21 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2010111509A1 (en) * 2009-03-25 2010-09-30 Life Technologies Corporation Discriminatory positive/extraction control dna
EP2660332A1 (de) * 2012-05-01 2013-11-06 F. Hoffmann-La Roche AG Positives Steuerkonzept
WO2014063051A2 (en) * 2012-10-18 2014-04-24 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEHETS J ET AL: "Development and evaluation of a Taqman duplex real-time PCR quantification method for reliable enumeration of Legionella pneumophila in water samples", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 68, no. 1, 1 January 2007 (2007-01-01), pages 137 - 144, XP022731911, ISSN: 0167-7012, [retrieved on 20070110], DOI: 10.1016/J.MIMET.2006.07.002 *
SATOKO NAKANO ET AL: "Establishment of Multiplex Solid-Phase Strip PCR Test for Detection of 24 Ocular Infectious Disease Pathogens", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, vol. 58, no. 3, 10 March 2017 (2017-03-10), US, pages 1553, XP055450489, ISSN: 1552-5783, DOI: 10.1167/iovs.16-20556 *
See also references of WO2016059798A1 *

Also Published As

Publication number Publication date
JPWO2016059798A1 (ja) 2017-08-10
WO2016059798A1 (ja) 2016-04-21
US20180087106A1 (en) 2018-03-29
JP6778374B2 (ja) 2020-11-04
EP3208333B1 (de) 2020-12-16
EP3208333A1 (de) 2017-08-23

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